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The map label for this gene is ykoW [H]

Identifier: 192289279

GI number: 192289279

Start: 900583

End: 902400

Strand: Reverse

Name: ykoW [H]

Synonym: Rpal_0851

Alternate gene names: 192289279

Gene position: 902400-900583 (Counterclockwise)

Preceding gene: 192289281

Following gene: 192289278

Centisome position: 15.71

GC content: 64.52

Gene sequence:

>1818_bases
ATGACGAGCGCCGCACCATCCTCTCCCTCAACTGTGACGACCGACGTCGCCGAGAGCGATGAGGCGCCTTCCACGGACGC
GGCCGGCATCGCGGCTGCAGAAACCGGGGACCTCCGGCTGTTCAAGGAAATCGTGCGCGCGCTGCCTTCGGCCGTCACGG
TCCAGGACGAGCAAGGCCGCTTCATCTTCATCAACGACGCGGCCGCCGCACAATATCGGCTTGCGCCTTCATCGACCGAC
GCCGCCGGCAACCTTCCGCTCGACCGACGCCGTAGCGAGGGCGCCGCGCTGCTCGCGCAGGGCCAGTCCGGCACGCGCGA
GGAACGCATCACCTTCGGCGGCGCGACCCGCGCGCTGCTGACCGCGCACAAGCCGATGGATATCGCGGGGCGCAAACTGC
TGCTGTCGAGCTCCCTCGACGTTACCGAGCAGAAGGTGATCGAGGACGAGCTGTTCCGCCGGGCCTATTACGACGAGCTC
ACCGGCCTGCCCAACCGCGCCGTGATCGAGCGCCACGTCACCGAGCTGCTCGAGGACCACCGGCCGCACCATTTCGCGCT
GGCGTTTCTCGACGTCGACAACTTCAAGCACATCAACGATTACTACGGCCATTCGGTGGGCGACGCCCTGCTGGTCGGCA
TCGCCAAGCGGCTGTCGCTGGAGCTGCGCGACACCGACGTGCTGTCGCGCATCAGCGGCGACGAATTCCTGCTGCTGCTC
GATCCGATCACCAGCGAAGCGGAAGTCGCCGAGCACATCAACTTCCTGCTGCAGCGACTGAAGGCCCCGTTCTACATCGA
CGGCACTGAAGTGTTCGCGTCGGCCTCGATCGGCATTAGCCTGTATCCCGAGCACGGCCGCAGCTACGAGGCGCTGCGGC
AGAACGCCGACATCGCGATGTACCGGGTCAAGAGCGACGGCAAAGGCGCCTCGGCGATCTTCAACATTGCGATGGAGCGC
GAGGCGTTGGAGCGGATGGCGGTGGAGCAGTCGCTGCGCCAGGCCATTCTCGACAGGCGCTTCTGCTGCGCATTCCAGCC
CAAGGTCGACATCCGCACCAACGAAATCAAAGGCGTCGAAGCTCTGGTGCGGCTGCGCGACGATAACGGCGTGATTCAGG
CGCCGGGCACCTTCGTCGATCTCGCGGTCGAACTCGGCCTGATCGACGAGTTGACCCATCTGGTGCTCGCCGAGATCATG
AAATCGATCGACCTGATCAACGCCACCTTCGGCGAGGACGCCAGCATCAGCGTCAATGTCGCCGCCAAGCAGGCCGGCTC
GCCGGACTTCATGCGCTCGTTCTGCGAAGCGCTGGCCGCAACCGAATGTCCGAAGCGGTTCATGGTGGAGATCACCGAGG
ACGCATTCGTCGCCAAGGCACATTTTCAGAAGCAGATCCTGCCGATGCTGCGGGAGATCGGCGTCGGCATTTCGATCGAC
GATTTCGGCATCGGCTACTCGTCGCTGTCGGCGCTTGCCGATATCACCGCCGACGAGATCAAGATCGACCGTTCGTTCAT
CACGGACATTCACAAGCGACCGCGCAGCCAGGGCATTTTGCGCGCGATCGAGTCGCTCAGCGACGCACTCGGCATGACGG
TGATCGCCGAAGGGCTCGAGACCTTCGAAGAGCTCGCTTACCTGCAGGCAATGACACGTATCCGCTACGCGCAGGGCTAC
TACTACTCCAAGCCGATCTTCCTCGAAGAATTGCGGCCGACCATTCCGGTGTTCAGCGGTGGCCGCCCCGGCGGGCCCGG
CCGTCACCTCGAACAAGCGCGTCAGCCGCTGACCCGGGCCACCTACGCGCGGCGCTGA

Upstream 100 bases:

>100_bases
ACTCGACTGCAATTCAAATCCACTGCGGATACGGGGATTGCGCCGGCATCCAGGGGGCACTTAACCATTATTACAGCGGC
CTTCGCCATCCTTCGCGCGC

Downstream 100 bases:

>100_bases
CCGCGCGATCAGCACGGCGCTAAGCGGTCATAGAACCGCATTCCGCACAGCGAACAAACACCTGATTTTGCTGACTTGTC
GGCCGTTACCTCCGGTGATG

Product: diguanylate cyclase/phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 605; Mature: 604

Protein sequence:

>605_residues
MTSAAPSSPSTVTTDVAESDEAPSTDAAGIAAAETGDLRLFKEIVRALPSAVTVQDEQGRFIFINDAAAAQYRLAPSSTD
AAGNLPLDRRRSEGAALLAQGQSGTREERITFGGATRALLTAHKPMDIAGRKLLLSSSLDVTEQKVIEDELFRRAYYDEL
TGLPNRAVIERHVTELLEDHRPHHFALAFLDVDNFKHINDYYGHSVGDALLVGIAKRLSLELRDTDVLSRISGDEFLLLL
DPITSEAEVAEHINFLLQRLKAPFYIDGTEVFASASIGISLYPEHGRSYEALRQNADIAMYRVKSDGKGASAIFNIAMER
EALERMAVEQSLRQAILDRRFCCAFQPKVDIRTNEIKGVEALVRLRDDNGVIQAPGTFVDLAVELGLIDELTHLVLAEIM
KSIDLINATFGEDASISVNVAAKQAGSPDFMRSFCEALAATECPKRFMVEITEDAFVAKAHFQKQILPMLREIGVGISID
DFGIGYSSLSALADITADEIKIDRSFITDIHKRPRSQGILRAIESLSDALGMTVIAEGLETFEELAYLQAMTRIRYAQGY
YYSKPIFLEELRPTIPVFSGGRPGGPGRHLEQARQPLTRATYARR

Sequences:

>Translated_605_residues
MTSAAPSSPSTVTTDVAESDEAPSTDAAGIAAAETGDLRLFKEIVRALPSAVTVQDEQGRFIFINDAAAAQYRLAPSSTD
AAGNLPLDRRRSEGAALLAQGQSGTREERITFGGATRALLTAHKPMDIAGRKLLLSSSLDVTEQKVIEDELFRRAYYDEL
TGLPNRAVIERHVTELLEDHRPHHFALAFLDVDNFKHINDYYGHSVGDALLVGIAKRLSLELRDTDVLSRISGDEFLLLL
DPITSEAEVAEHINFLLQRLKAPFYIDGTEVFASASIGISLYPEHGRSYEALRQNADIAMYRVKSDGKGASAIFNIAMER
EALERMAVEQSLRQAILDRRFCCAFQPKVDIRTNEIKGVEALVRLRDDNGVIQAPGTFVDLAVELGLIDELTHLVLAEIM
KSIDLINATFGEDASISVNVAAKQAGSPDFMRSFCEALAATECPKRFMVEITEDAFVAKAHFQKQILPMLREIGVGISID
DFGIGYSSLSALADITADEIKIDRSFITDIHKRPRSQGILRAIESLSDALGMTVIAEGLETFEELAYLQAMTRIRYAQGY
YYSKPIFLEELRPTIPVFSGGRPGGPGRHLEQARQPLTRATYARR
>Mature_604_residues
TSAAPSSPSTVTTDVAESDEAPSTDAAGIAAAETGDLRLFKEIVRALPSAVTVQDEQGRFIFINDAAAAQYRLAPSSTDA
AGNLPLDRRRSEGAALLAQGQSGTREERITFGGATRALLTAHKPMDIAGRKLLLSSSLDVTEQKVIEDELFRRAYYDELT
GLPNRAVIERHVTELLEDHRPHHFALAFLDVDNFKHINDYYGHSVGDALLVGIAKRLSLELRDTDVLSRISGDEFLLLLD
PITSEAEVAEHINFLLQRLKAPFYIDGTEVFASASIGISLYPEHGRSYEALRQNADIAMYRVKSDGKGASAIFNIAMERE
ALERMAVEQSLRQAILDRRFCCAFQPKVDIRTNEIKGVEALVRLRDDNGVIQAPGTFVDLAVELGLIDELTHLVLAEIMK
SIDLINATFGEDASISVNVAAKQAGSPDFMRSFCEALAATECPKRFMVEITEDAFVAKAHFQKQILPMLREIGVGISIDD
FGIGYSSLSALADITADEIKIDRSFITDIHKRPRSQGILRAIESLSDALGMTVIAEGLETFEELAYLQAMTRIRYAQGYY
YSKPIFLEELRPTIPVFSGGRPGGPGRHLEQARQPLTRATYARR

Specific function: Probable signaling protein whose physiological role is not yet known [H]

COG id: COG5001

COG function: function code T; Predicted signal transduction protein containing a membrane domain, an EAL and a GGDEF domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1787541, Length=437, Percent_Identity=33.8672768878719, Blast_Score=251, Evalue=1e-67,
Organism=Escherichia coli, GI87081921, Length=418, Percent_Identity=29.4258373205742, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI87081845, Length=242, Percent_Identity=34.7107438016529, Blast_Score=131, Evalue=2e-31,
Organism=Escherichia coli, GI1790496, Length=240, Percent_Identity=31.25, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI226510982, Length=440, Percent_Identity=24.7727272727273, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI87081881, Length=204, Percent_Identity=36.7647058823529, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI87081743, Length=237, Percent_Identity=31.2236286919831, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1788502, Length=250, Percent_Identity=30, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1787055, Length=250, Percent_Identity=32.8, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1788381, Length=408, Percent_Identity=26.4705882352941, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1788956, Length=157, Percent_Identity=39.4904458598726, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI87081980, Length=266, Percent_Identity=27.4436090225564, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI87081977, Length=159, Percent_Identity=36.4779874213836, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788849, Length=439, Percent_Identity=24.6013667425968, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI87082007, Length=175, Percent_Identity=35.4285714285714, Blast_Score=86, Evalue=9e-18,
Organism=Escherichia coli, GI87082096, Length=427, Percent_Identity=25.9953161592506, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1786507, Length=289, Percent_Identity=24.2214532871972, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1786584, Length=160, Percent_Identity=28.75, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787410, Length=229, Percent_Identity=27.5109170305677, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1787816, Length=151, Percent_Identity=31.1258278145695, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI145693134, Length=170, Percent_Identity=31.7647058823529, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1787262, Length=163, Percent_Identity=28.8343558282209, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330
- InterPro:   IPR000014 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT [H]

EC number: NA

Molecular weight: Translated: 66581; Mature: 66450

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSAAPSSPSTVTTDVAESDEAPSTDAAGIAAAETGDLRLFKEIVRALPSAVTVQDEQGR
CCCCCCCCCCCEEECCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCEEEEECCCCC
FIFINDAAAAQYRLAPSSTDAAGNLPLDRRRSEGAALLAQGQSGTREERITFGGATRALL
EEEEECCCCCEEEECCCCCCCCCCCCCHHCCCCCCEEEECCCCCCCCCEEEECCCHHHHH
TAHKPMDIAGRKLLLSSSLDVTEQKVIEDELFRRAYYDELTGLPNRAVIERHVTELLEDH
HCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
RPHHFALAFLDVDNFKHINDYYGHSVGDALLVGIAKRLSLELRDTDVLSRISGDEFLLLL
CCCEEEEEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHEEEECCHHHHHHCCCCCEEEEE
DPITSEAEVAEHINFLLQRLKAPFYIDGTEVFASASIGISLYPEHGRSYEALRQNADIAM
CCCCCHHHHHHHHHHHHHHHCCCEEECCHHHHHEECCCEEEECCCCCCHHHHHCCCCEEE
YRVKSDGKGASAIFNIAMEREALERMAVEQSLRQAILDRRFCCAFQPKVDIRTNEIKGVE
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEECCCCCCCCCCHHHHHH
ALVRLRDDNGVIQAPGTFVDLAVELGLIDELTHLVLAEIMKSIDLINATFGEDASISVNV
HHHEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
AAKQAGSPDFMRSFCEALAATECPKRFMVEITEDAFVAKAHFQKQILPMLREIGVGISID
EECCCCCHHHHHHHHHHHHHHCCCHHHHHEECCHHHHHHHHHHHHHHHHHHHHCCCCEEC
DFGIGYSSLSALADITADEIKIDRSFITDIHKRPRSQGILRAIESLSDALGMTVIAEGLE
CCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TFEELAYLQAMTRIRYAQGYYYSKPIFLEELRPTIPVFSGGRPGGPGRHLEQARQPLTRA
HHHHHHHHHHHHHHHHHCCEEECCCEEHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TYARR
HHCCC
>Mature Secondary Structure 
TSAAPSSPSTVTTDVAESDEAPSTDAAGIAAAETGDLRLFKEIVRALPSAVTVQDEQGR
CCCCCCCCCCEEECCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCEEEEECCCCC
FIFINDAAAAQYRLAPSSTDAAGNLPLDRRRSEGAALLAQGQSGTREERITFGGATRALL
EEEEECCCCCEEEECCCCCCCCCCCCCHHCCCCCCEEEECCCCCCCCCEEEECCCHHHHH
TAHKPMDIAGRKLLLSSSLDVTEQKVIEDELFRRAYYDELTGLPNRAVIERHVTELLEDH
HCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
RPHHFALAFLDVDNFKHINDYYGHSVGDALLVGIAKRLSLELRDTDVLSRISGDEFLLLL
CCCEEEEEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHEEEECCHHHHHHCCCCCEEEEE
DPITSEAEVAEHINFLLQRLKAPFYIDGTEVFASASIGISLYPEHGRSYEALRQNADIAM
CCCCCHHHHHHHHHHHHHHHCCCEEECCHHHHHEECCCEEEECCCCCCHHHHHCCCCEEE
YRVKSDGKGASAIFNIAMEREALERMAVEQSLRQAILDRRFCCAFQPKVDIRTNEIKGVE
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEECCCCCCCCCCHHHHHH
ALVRLRDDNGVIQAPGTFVDLAVELGLIDELTHLVLAEIMKSIDLINATFGEDASISVNV
HHHEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
AAKQAGSPDFMRSFCEALAATECPKRFMVEITEDAFVAKAHFQKQILPMLREIGVGISID
EECCCCCHHHHHHHHHHHHHHCCCHHHHHEECCHHHHHHHHHHHHHHHHHHHHCCCCEEC
DFGIGYSSLSALADITADEIKIDRSFITDIHKRPRSQGILRAIESLSDALGMTVIAEGLE
CCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TFEELAYLQAMTRIRYAQGYYYSKPIFLEELRPTIPVFSGGRPGGPGRHLEQARQPLTRA
HHHHHHHHHHHHHHHHHCCEEECCCEEHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TYARR
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 11728710 [H]