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The map label for this gene is menB [H]

Identifier: 192289150

GI number: 192289150

Start: 755704

End: 756486

Strand: Reverse

Name: menB [H]

Synonym: Rpal_0720

Alternate gene names: 192289150

Gene position: 756486-755704 (Counterclockwise)

Preceding gene: 192289151

Following gene: 192289149

Centisome position: 13.17

GC content: 62.96

Gene sequence:

>783_bases
ATGCAGTTCGAAGACCTGATCTATGAGATCCGCAACGGCGTGGCGTGGATCATCATCAATCGTCCGGACAAGATGAACGC
GTTCCGCGGCACCACCTGCGACGAGTTGATCAAGGCACTGTACAAGGCCGGCTACGACAAGGACGTCGGCGCGATCGTGC
TCGCCGGCGCCGGCGACCGCGCCTTCTGCACCGGCGGCGATCAATCGACCCACGACGGCAATTACGATGGCCGAGGCACC
GTCGGGCTGCCGATGGAGGAACTGCACACTGCGATCCGCGACGTGCCGAAGCCGGTGATCGCCCGCGTGCAGGGCTATGC
GATCGGCGGCGGCAACGTGCTGGCAACAATCTGCGATCTGACGATCTGCTCGGAGAAAGCGATCTTCGGCCAGGTCGGCC
CGAAGATGGGCTCGGTCGATCCGGGCTACGGCACCGCGTTCCTCGCCCGCGTCGTCGGCGAGAAGAAGGCCCGCGAGATT
TGGTATATGTGCCGGCGCTACTCGGGCAAAGAAGCCGAGGCAATGGGCCTCGCCAATCTCTGCGTGCCGCACGACGAGCT
CGACGCCGAAGTACAAAAGTGGGGCGAGGAACTGTGCGAGCGGTCACCGACCGCACTCGCCATCGCCAAGCGCAGCTTCA
ACATGGACACCGCGCATCAAGCCGGCATTGCCGGCATGGGGATGTACGCGCTGAAGCTCTATTACGACACCGAGGAATCC
CGCGAAGGCGTCAAGGCGCTGCAGGAGAAGCGCAAACCCGAGTTCCGCAAGTACATCAAATAA

Upstream 100 bases:

>100_bases
GGCAGCGACGACGCCGGCTTCATCACCGGGCAGGTGCTGAGCGTCTCCGGCGGCCTGACGATGAACGGCTGAACCAATCA
ACAACAACGGGAGGATTCCG

Downstream 100 bases:

>100_bases
TTGCTCTCCGCCGACGAACGGCGCCGGCAACGCCCGGCGCTCGAATTCTCAAGGAGGGCGAGGCATGAATCCATATTTGA
ACGAAGACCTCGTGGCGCTG

Product: 2-ketocyclohexanecarboxyl-CoA hydrolase

Products: NA

Alternate protein names: DHNA-CoA synthase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MQFEDLIYEIRNGVAWIIINRPDKMNAFRGTTCDELIKALYKAGYDKDVGAIVLAGAGDRAFCTGGDQSTHDGNYDGRGT
VGLPMEELHTAIRDVPKPVIARVQGYAIGGGNVLATICDLTICSEKAIFGQVGPKMGSVDPGYGTAFLARVVGEKKAREI
WYMCRRYSGKEAEAMGLANLCVPHDELDAEVQKWGEELCERSPTALAIAKRSFNMDTAHQAGIAGMGMYALKLYYDTEES
REGVKALQEKRKPEFRKYIK

Sequences:

>Translated_260_residues
MQFEDLIYEIRNGVAWIIINRPDKMNAFRGTTCDELIKALYKAGYDKDVGAIVLAGAGDRAFCTGGDQSTHDGNYDGRGT
VGLPMEELHTAIRDVPKPVIARVQGYAIGGGNVLATICDLTICSEKAIFGQVGPKMGSVDPGYGTAFLARVVGEKKAREI
WYMCRRYSGKEAEAMGLANLCVPHDELDAEVQKWGEELCERSPTALAIAKRSFNMDTAHQAGIAGMGMYALKLYYDTEES
REGVKALQEKRKPEFRKYIK
>Mature_260_residues
MQFEDLIYEIRNGVAWIIINRPDKMNAFRGTTCDELIKALYKAGYDKDVGAIVLAGAGDRAFCTGGDQSTHDGNYDGRGT
VGLPMEELHTAIRDVPKPVIARVQGYAIGGGNVLATICDLTICSEKAIFGQVGPKMGSVDPGYGTAFLARVVGEKKAREI
WYMCRRYSGKEAEAMGLANLCVPHDELDAEVQKWGEELCERSPTALAIAKRSFNMDTAHQAGIAGMGMYALKLYYDTEES
REGVKALQEKRKPEFRKYIK

Specific function: Converts o-succinylbenzoyl-CoA (OSB-CoA) to 1,4- dihydroxy-2-naphthoyl-CoA (DHNA-CoA) [H]

COG id: COG0447

COG function: function code H; Dihydroxynaphthoic acid synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=261, Percent_Identity=35.2490421455939, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI31542718, Length=254, Percent_Identity=26.7716535433071, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI260274832, Length=213, Percent_Identity=27.2300469483568, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI45643119, Length=213, Percent_Identity=27.2300469483568, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI260275230, Length=213, Percent_Identity=27.2300469483568, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4502327, Length=260, Percent_Identity=29.6153846153846, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI70995211, Length=257, Percent_Identity=27.6264591439689, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI157694516, Length=206, Percent_Identity=24.2718446601942, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI213417737, Length=206, Percent_Identity=24.2718446601942, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI68989263, Length=183, Percent_Identity=28.9617486338798, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1788597, Length=260, Percent_Identity=48.4615384615385, Blast_Score=251, Evalue=3e-68,
Organism=Escherichia coli, GI1787659, Length=256, Percent_Identity=28.515625, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI87082183, Length=253, Percent_Identity=27.2727272727273, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI221142681, Length=264, Percent_Identity=28.4090909090909, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787660, Length=261, Percent_Identity=27.5862068965517, Blast_Score=80, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17554946, Length=248, Percent_Identity=34.6774193548387, Blast_Score=133, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI25145438, Length=260, Percent_Identity=31.1538461538462, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17540714, Length=206, Percent_Identity=29.6116504854369, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17535521, Length=203, Percent_Identity=27.0935960591133, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17549921, Length=209, Percent_Identity=24.8803827751196, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17536985, Length=238, Percent_Identity=25.6302521008403, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17560910, Length=221, Percent_Identity=28.0542986425339, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI20129971, Length=244, Percent_Identity=34.8360655737705, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24653477, Length=244, Percent_Identity=34.8360655737705, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24653139, Length=255, Percent_Identity=29.4117647058824, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19922422, Length=247, Percent_Identity=23.8866396761134, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21357171, Length=259, Percent_Identity=25.8687258687259, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24654903, Length=212, Percent_Identity=25.4716981132075, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24650670, Length=180, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376
- InterPro:   IPR010198 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.1.3.36 [H]

Molecular weight: Translated: 28618; Mature: 28618

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFEDLIYEIRNGVAWIIINRPDKMNAFRGTTCDELIKALYKAGYDKDVGAIVLAGAGDR
CCHHHHHHHHHCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCC
AFCTGGDQSTHDGNYDGRGTVGLPMEELHTAIRDVPKPVIARVQGYAIGGGNVLATICDL
EEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHCCEEECCCHHHHHHHHH
TICSEKAIFGQVGPKMGSVDPGYGTAFLARVVGEKKAREIWYMCRRYSGKEAEAMGLANL
HHHCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
CVPHDELDAEVQKWGEELCERSPTALAIAKRSFNMDTAHQAGIAGMGMYALKLYYDTEES
CCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCHHHHCCCCCCCEEEEEEEECCHHH
REGVKALQEKRKPEFRKYIK
HHHHHHHHHHCCHHHHHHCC
>Mature Secondary Structure
MQFEDLIYEIRNGVAWIIINRPDKMNAFRGTTCDELIKALYKAGYDKDVGAIVLAGAGDR
CCHHHHHHHHHCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCC
AFCTGGDQSTHDGNYDGRGTVGLPMEELHTAIRDVPKPVIARVQGYAIGGGNVLATICDL
EEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHCCEEECCCHHHHHHHHH
TICSEKAIFGQVGPKMGSVDPGYGTAFLARVVGEKKAREIWYMCRRYSGKEAEAMGLANL
HHHCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
CVPHDELDAEVQKWGEELCERSPTALAIAKRSFNMDTAHQAGIAGMGMYALKLYYDTEES
CCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCHHHHCCCCCCCEEEEEEEECCHHH
REGVKALQEKRKPEFRKYIK
HHHHHHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1629163; 8566759; 9387221; 9384377 [H]