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The map label for this gene is mdeA [H]

Identifier: 192288806

GI number: 192288806

Start: 399947

End: 401131

Strand: Reverse

Name: mdeA [H]

Synonym: Rpal_0375

Alternate gene names: 192288806

Gene position: 401131-399947 (Counterclockwise)

Preceding gene: 192288808

Following gene: 192288805

Centisome position: 6.98

GC content: 65.99

Gene sequence:

>1185_bases
ATGACCGAATCGTCCCACTCGCCCGGCTTTGCCACCACCGCGATCCACCACGGCTACGACCCGCTGGCGCATCACGGTGC
GCTCAACCCACCGCTGTTCCTGACCTCGACCTACGCGTTCGCCAGCGCCGCTGAGGGAGCGGCGCGGTTCGCCGGCGAGG
CCGAAGGCTTCGTGTATTCGCGGGTCGGCAATCCGACGGTGGCGGTGCTGGAGAGCCGACTGGCGGCGCTGGAAGGCGGC
GAGGCCGCGCTCGCCACCGCCTCGGGCGTCGGGGCCCTGACGGCGCTGATCTGGACGCTGCTGAAGACGGGCGACGGCAT
CATCGCCGACCAGATGCTGTACGGCTGCACCTTCGGTCTGTTCGAGCATCATCTGCCACGGTTCGGCATCGAGGTGGTGT
TCGCCGATCTCACCGATCCGGCCAATCTGCAGGCCGCGCTGCGGCCGAATACCCGGCTGGTGTTCACCGAGACCCCGACC
AATCCGAATATGCGGCTGGTCGACATCGCCGCCTGCGCCGAGATCTGCCGGCGCGCCGACGTTCGCCTCGTCGTCGACAA
CACCTACTGTACGCCGTATCTGCAGCGCCCGCTCGATCTCGGCGCAGATCTCGTCACCCATTCAGCGACGAAGTATCTCG
GCGGCCACGGCGATCTTCTGGCCGGCGCGGTGGTGGGGCCGAAGGAGATCATCGATCAGCTCCGCTTCGTCGGCGTTAAG
GAGCTGAACGGTGCCTGCATCTCGGCGTTCGACGCCTTCCTGATCCTGCGCGGCCTGAAGACGTTGAACCTGCGCATGGA
TCGCCACAGCGCCACCGCCGTAAAGCTCGCTCACGATCTCGAAGCGCATCCCGCCGTCGCCGCCGTGTACTACCCCGGTC
TCGACAGCCACAAGCAGCGCAACCTCGCGCAGCGGCAGATGCGCCAGCCCGGCGGCATGATCGCACTCGAACTCAGAGGC
GGATTAGCAGCCGGCCGCAGCTTCATGGACAAGGTGAAGCTGGCGACGCGAGCGGTGAGTCTCGGCGACGCCGAGACCCT
GATCCAACACCCGGCCAGCATGACCCACGCGACCTACGATCCGGCCGAACGCGCGCGGCACGGATTTACGGACGGATTGA
TCAGGATCTCAGTAGGACTGGAGGATTATGAGGATCTGAAACACGACTTGCTTGGGGCGCTATGA

Upstream 100 bases:

>100_bases
GCAAAGTGAAGAAGATTGCCAATTTGTATGTGTTTGATGGCAATGATTGGCAAGTTTAGAAGCGGGCTTCGGGCCATACT
CGCCAAAAAGGAGCCCCGCC

Downstream 100 bases:

>100_bases
ACTACGCGTTGTTCCGACCATGCGGAACCCGAAGTTCCAACCTCGATCTACTCCGGCGCGGCCACGCCGGTTCCAAGTCG
GTTCACGCAAATTATACCAC

Product: methionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MTESSHSPGFATTAIHHGYDPLAHHGALNPPLFLTSTYAFASAAEGAARFAGEAEGFVYSRVGNPTVAVLESRLAALEGG
EAALATASGVGALTALIWTLLKTGDGIIADQMLYGCTFGLFEHHLPRFGIEVVFADLTDPANLQAALRPNTRLVFTETPT
NPNMRLVDIAACAEICRRADVRLVVDNTYCTPYLQRPLDLGADLVTHSATKYLGGHGDLLAGAVVGPKEIIDQLRFVGVK
ELNGACISAFDAFLILRGLKTLNLRMDRHSATAVKLAHDLEAHPAVAAVYYPGLDSHKQRNLAQRQMRQPGGMIALELRG
GLAAGRSFMDKVKLATRAVSLGDAETLIQHPASMTHATYDPAERARHGFTDGLIRISVGLEDYEDLKHDLLGAL

Sequences:

>Translated_394_residues
MTESSHSPGFATTAIHHGYDPLAHHGALNPPLFLTSTYAFASAAEGAARFAGEAEGFVYSRVGNPTVAVLESRLAALEGG
EAALATASGVGALTALIWTLLKTGDGIIADQMLYGCTFGLFEHHLPRFGIEVVFADLTDPANLQAALRPNTRLVFTETPT
NPNMRLVDIAACAEICRRADVRLVVDNTYCTPYLQRPLDLGADLVTHSATKYLGGHGDLLAGAVVGPKEIIDQLRFVGVK
ELNGACISAFDAFLILRGLKTLNLRMDRHSATAVKLAHDLEAHPAVAAVYYPGLDSHKQRNLAQRQMRQPGGMIALELRG
GLAAGRSFMDKVKLATRAVSLGDAETLIQHPASMTHATYDPAERARHGFTDGLIRISVGLEDYEDLKHDLLGAL
>Mature_393_residues
TESSHSPGFATTAIHHGYDPLAHHGALNPPLFLTSTYAFASAAEGAARFAGEAEGFVYSRVGNPTVAVLESRLAALEGGE
AALATASGVGALTALIWTLLKTGDGIIADQMLYGCTFGLFEHHLPRFGIEVVFADLTDPANLQAALRPNTRLVFTETPTN
PNMRLVDIAACAEICRRADVRLVVDNTYCTPYLQRPLDLGADLVTHSATKYLGGHGDLLAGAVVGPKEIIDQLRFVGVKE
LNGACISAFDAFLILRGLKTLNLRMDRHSATAVKLAHDLEAHPAVAAVYYPGLDSHKQRNLAQRQMRQPGGMIALELRGG
LAAGRSFMDKVKLATRAVSLGDAETLIQHPASMTHATYDPAERARHGFTDGLIRISVGLEDYEDLKHDLLGAL

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=378, Percent_Identity=43.6507936507937, Blast_Score=275, Evalue=8e-74,
Organism=Homo sapiens, GI299473759, Length=378, Percent_Identity=39.4179894179894, Blast_Score=241, Evalue=1e-63,
Organism=Homo sapiens, GI25453487, Length=377, Percent_Identity=37.6657824933687, Blast_Score=202, Evalue=3e-52,
Organism=Escherichia coli, GI1790375, Length=381, Percent_Identity=39.8950131233596, Blast_Score=251, Evalue=5e-68,
Organism=Escherichia coli, GI1789383, Length=371, Percent_Identity=30.188679245283, Blast_Score=149, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17560056, Length=379, Percent_Identity=41.9525065963061, Blast_Score=279, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17538055, Length=378, Percent_Identity=42.3280423280423, Blast_Score=278, Evalue=4e-75,
Organism=Caenorhabditis elegans, GI71980708, Length=383, Percent_Identity=37.3368146214099, Blast_Score=243, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6319307, Length=397, Percent_Identity=39.0428211586902, Blast_Score=235, Evalue=8e-63,
Organism=Saccharomyces cerevisiae, GI6323333, Length=423, Percent_Identity=34.0425531914894, Blast_Score=198, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6321254, Length=396, Percent_Identity=32.5757575757576, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6321170, Length=327, Percent_Identity=29.0519877675841, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6321904, Length=397, Percent_Identity=27.9596977329975, Blast_Score=102, Evalue=7e-23,
Organism=Drosophila melanogaster, GI19922554, Length=398, Percent_Identity=39.9497487437186, Blast_Score=274, Evalue=6e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 42183; Mature: 42051

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESSHSPGFATTAIHHGYDPLAHHGALNPPLFLTSTYAFASAAEGAARFAGEAEGFVYS
CCCCCCCCCCEEHHHHCCCCHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCEEEE
RVGNPTVAVLESRLAALEGGEAALATASGVGALTALIWTLLKTGDGIIADQMLYGCTFGL
CCCCCHHHHHHHHHHHHCCCCEEEEHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FEHHLPRFGIEVVFADLTDPANLQAALRPNTRLVFTETPTNPNMRLVDIAACAEICRRAD
HHHCCCCCCEEEEEEECCCCCCCEEEECCCCEEEEEECCCCCCEEEEEHHHHHHHHHCCC
VRLVVDNTYCTPYLQRPLDLGADLVTHSATKYLGGHGDLLAGAVVGPKEIIDQLRFVGVK
CEEEEECCCCCHHHHCHHHCCHHHHHHHHHHHCCCCCCCHHCCCCCHHHHHHHHHHCCHH
ELNGACISAFDAFLILRGLKTLNLRMDRHSATAVKLAHDLEAHPAVAAVYYPGLDSHKQR
HCCCHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHH
NLAQRQMRQPGGMIALELRGGLAAGRSFMDKVKLATRAVSLGDAETLIQHPASMTHATYD
HHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHCCCCCC
PAERARHGFTDGLIRISVGLEDYEDLKHDLLGAL
HHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TESSHSPGFATTAIHHGYDPLAHHGALNPPLFLTSTYAFASAAEGAARFAGEAEGFVYS
CCCCCCCCCEEHHHHCCCCHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCEEEE
RVGNPTVAVLESRLAALEGGEAALATASGVGALTALIWTLLKTGDGIIADQMLYGCTFGL
CCCCCHHHHHHHHHHHHCCCCEEEEHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FEHHLPRFGIEVVFADLTDPANLQAALRPNTRLVFTETPTNPNMRLVDIAACAEICRRAD
HHHCCCCCCEEEEEEECCCCCCCEEEECCCCEEEEEECCCCCCEEEEEHHHHHHHHHCCC
VRLVVDNTYCTPYLQRPLDLGADLVTHSATKYLGGHGDLLAGAVVGPKEIIDQLRFVGVK
CEEEEECCCCCHHHHCHHHCCHHHHHHHHHHHCCCCCCCHHCCCCCHHHHHHHHHHCCHH
ELNGACISAFDAFLILRGLKTLNLRMDRHSATAVKLAHDLEAHPAVAAVYYPGLDSHKQR
HCCCHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHH
NLAQRQMRQPGGMIALELRGGLAAGRSFMDKVKLATRAVSLGDAETLIQHPASMTHATYD
HHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHCCCCCC
PAERARHGFTDGLIRISVGLEDYEDLKHDLLGAL
HHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]