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The map label for this gene is lepA [H]

Identifier: 192288786

GI number: 192288786

Start: 374910

End: 376721

Strand: Reverse

Name: lepA [H]

Synonym: Rpal_0355

Alternate gene names: 192288786

Gene position: 376721-374910 (Counterclockwise)

Preceding gene: 192288788

Following gene: 192288782

Centisome position: 6.56

GC content: 63.63

Gene sequence:

>1812_bases
ATGACAACCGCCCCCATCGACAACATCCGCAACTTCTCCATCGTCGCCCATATCGACCATGGCAAGTCGACGCTCGCCGA
CCGCCTGATCCAGATCACCGGCGGCATGTCCGACCGTGAAATGGCCGGCAAGGAGCAGGTGCTCGATTCGATGGACATCG
AGCGTGAGCGCGGCATCACCATCAAGGCGCAGACCGTTCGTCTGAAATACCGCGCCCATGACGGCAAGGACTACATCTTC
AACCTGATGGACACGCCCGGCCACGTCGACTTCGCCTACGAAGTGTCGCGGTCGCTGGCGGCGTGCGAAGGCTCGCTGCT
GGTGGTGGACGCCAGCCAGGGCGTCGAAGCGCAGACGCTCGCCAACGTCTATCACGCGCTCGACGCCGGCCACGAGATCG
TGCCCGTGCTCAACAAGGTCGATTTGCCGGCCGCCGAGCCCGAGAAGATCAAGCAGCAGATCGAGGACGTGATCGGCCTC
GACGCCTCCGACGCGGTGATGATCTCGGCCAAGACCGGCCTCGGCGTGCCCGACGTGCTGGAAGCGATCGTCACCCGGCT
GCCGCCGCCCAAGGGCGACCGCGACGCCACCCTGAAGGCGTTGCTGGTCGATAGCTGGTACGATGTCTATCTCGGCGTCG
TGGTGCTGGTGCGGGTCGTCGATGGCGTGCTCAAGAAGGGCCAGCGCATCCGGATGATGGGCACCGGCGCCGCCTACGAC
GTCGAGCGCGTCGGCTATTTCACGCCCAAGATGGTCAACGTCGAAGAACTCGGCCCCGGCGAAGTCGGCTTCATCACCGC
GGCGATCAAGGAAGTTGCCGACACCCGCGTCGGCGACACCATCACTGACGACAAGAAGCCGGTTACCGACATGCTGCCGG
GCTTCAAGCCGGCGATCCCGGTGGTGTTCTGCGGCCTGTTCCCGGTCGACGCCGACGATTTCGAGACGCTCCGCGCCGCG
ATGGGCAAGCTGCGACTGAACGACGCCAGCTTCTCGTTCGAGATGGAGACCTCGGCCGCGCTCGGCTTCGGCTTCCGCTG
CGGCTTCCTCGGCCTGCTGCATCTGGAGATCATCCAGGAGCGGCTCAGCCGCGAGTTCGACCTCGACCTGATCGCGACCG
CGCCGAGCGTGATCTACAAGATGAAGCTCACCGACGGCACCGAGATGGAGATCCACAATCCGGTCGACATGCCGGACGTG
GTGAAGATCGCCGAGATCGAGGAGCCGTGGATCGAAGCCACGATCCTGACGCCCGACGAATACTTGGGCTCGGTTCTGAA
GCTGTGCCAGGATCGCCGCGGCAATCAGAAAGAGCTGACCTACGTCGGCGCCCGCGCGATGGTGAAGTATGACTTGCCGC
TCAACGAGGTGGTGTTCGACTTCTACGACCGGCTGAAGAGCGTGTCGAAGGGCTACGCCTCGTTCGACTATCACCTCACC
GACTACAAGCCGGCCGATCTCGTCAAGATGCAGATCCTGGTCAATGCCGAGCCGGTCGACGCGCTGTCGATGCTGGTGCA
CCGGACGCGCGCCGAAGGCCGCGGCCGCGCCATGGTCGAGAAGATGAAAGAGCTGATCCCGCCGCACATGTTCCAGATCC
CGATCCAGGCGGCGATCGGCGGCAAGGTGATCGCCCGCGAAACCGTCCGCGCCCTGCGCAAGGACGTCACCGCCAAGTGC
TACGGCGGCGACATCACCCGAAAGCGCAAACTTCTGGAGAAACAGAAGGAGGGCAAGAAGAAGATGCGGCAGTTCGGCAA
GGTCGACATCCCACAGGAAGCGTTCATTGCCGCATTGAAGGTGGATAGTTGA

Upstream 100 bases:

>100_bases
TCCGCCGGTCGCGTCAAGCATCGCAAAACCGGCGGTTTTTCGGGAGGTTGCGGCTTCCGTTCGCCTTGCCCGGCCTGAGT
CGCGATGCTATCTGGCCGCA

Downstream 100 bases:

>100_bases
GGGACGTTACGTTTCGTCCTCGTCGTCCTCTGCCTCGGGCGCGGCCAATATTTCGGTTTCGCCCTCCGCCCATTTGAAGG
CGGATTTGTTGCGTGCGTCG

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 603; Mature: 602

Protein sequence:

>603_residues
MTTAPIDNIRNFSIVAHIDHGKSTLADRLIQITGGMSDREMAGKEQVLDSMDIERERGITIKAQTVRLKYRAHDGKDYIF
NLMDTPGHVDFAYEVSRSLAACEGSLLVVDASQGVEAQTLANVYHALDAGHEIVPVLNKVDLPAAEPEKIKQQIEDVIGL
DASDAVMISAKTGLGVPDVLEAIVTRLPPPKGDRDATLKALLVDSWYDVYLGVVVLVRVVDGVLKKGQRIRMMGTGAAYD
VERVGYFTPKMVNVEELGPGEVGFITAAIKEVADTRVGDTITDDKKPVTDMLPGFKPAIPVVFCGLFPVDADDFETLRAA
MGKLRLNDASFSFEMETSAALGFGFRCGFLGLLHLEIIQERLSREFDLDLIATAPSVIYKMKLTDGTEMEIHNPVDMPDV
VKIAEIEEPWIEATILTPDEYLGSVLKLCQDRRGNQKELTYVGARAMVKYDLPLNEVVFDFYDRLKSVSKGYASFDYHLT
DYKPADLVKMQILVNAEPVDALSMLVHRTRAEGRGRAMVEKMKELIPPHMFQIPIQAAIGGKVIARETVRALRKDVTAKC
YGGDITRKRKLLEKQKEGKKKMRQFGKVDIPQEAFIAALKVDS

Sequences:

>Translated_603_residues
MTTAPIDNIRNFSIVAHIDHGKSTLADRLIQITGGMSDREMAGKEQVLDSMDIERERGITIKAQTVRLKYRAHDGKDYIF
NLMDTPGHVDFAYEVSRSLAACEGSLLVVDASQGVEAQTLANVYHALDAGHEIVPVLNKVDLPAAEPEKIKQQIEDVIGL
DASDAVMISAKTGLGVPDVLEAIVTRLPPPKGDRDATLKALLVDSWYDVYLGVVVLVRVVDGVLKKGQRIRMMGTGAAYD
VERVGYFTPKMVNVEELGPGEVGFITAAIKEVADTRVGDTITDDKKPVTDMLPGFKPAIPVVFCGLFPVDADDFETLRAA
MGKLRLNDASFSFEMETSAALGFGFRCGFLGLLHLEIIQERLSREFDLDLIATAPSVIYKMKLTDGTEMEIHNPVDMPDV
VKIAEIEEPWIEATILTPDEYLGSVLKLCQDRRGNQKELTYVGARAMVKYDLPLNEVVFDFYDRLKSVSKGYASFDYHLT
DYKPADLVKMQILVNAEPVDALSMLVHRTRAEGRGRAMVEKMKELIPPHMFQIPIQAAIGGKVIARETVRALRKDVTAKC
YGGDITRKRKLLEKQKEGKKKMRQFGKVDIPQEAFIAALKVDS
>Mature_602_residues
TTAPIDNIRNFSIVAHIDHGKSTLADRLIQITGGMSDREMAGKEQVLDSMDIERERGITIKAQTVRLKYRAHDGKDYIFN
LMDTPGHVDFAYEVSRSLAACEGSLLVVDASQGVEAQTLANVYHALDAGHEIVPVLNKVDLPAAEPEKIKQQIEDVIGLD
ASDAVMISAKTGLGVPDVLEAIVTRLPPPKGDRDATLKALLVDSWYDVYLGVVVLVRVVDGVLKKGQRIRMMGTGAAYDV
ERVGYFTPKMVNVEELGPGEVGFITAAIKEVADTRVGDTITDDKKPVTDMLPGFKPAIPVVFCGLFPVDADDFETLRAAM
GKLRLNDASFSFEMETSAALGFGFRCGFLGLLHLEIIQERLSREFDLDLIATAPSVIYKMKLTDGTEMEIHNPVDMPDVV
KIAEIEEPWIEATILTPDEYLGSVLKLCQDRRGNQKELTYVGARAMVKYDLPLNEVVFDFYDRLKSVSKGYASFDYHLTD
YKPADLVKMQILVNAEPVDALSMLVHRTRAEGRGRAMVEKMKELIPPHMFQIPIQAAIGGKVIARETVRALRKDVTAKCY
GGDITRKRKLLEKQKEGKKKMRQFGKVDIPQEAFIAALKVDS

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=612, Percent_Identity=49.5098039215686, Blast_Score=622, Evalue=1e-178,
Organism=Homo sapiens, GI94966754, Length=134, Percent_Identity=47.0149253731343, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI18390331, Length=163, Percent_Identity=36.8098159509202, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI4503483, Length=145, Percent_Identity=39.3103448275862, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI310132016, Length=111, Percent_Identity=45.945945945946, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI310110807, Length=111, Percent_Identity=45.945945945946, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI310123363, Length=111, Percent_Identity=45.945945945946, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI25306283, Length=140, Percent_Identity=46.4285714285714, Blast_Score=97, Evalue=6e-20,
Organism=Homo sapiens, GI25306287, Length=140, Percent_Identity=46.4285714285714, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI19923640, Length=140, Percent_Identity=46.4285714285714, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI217272894, Length=160, Percent_Identity=33.125, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI217272892, Length=160, Percent_Identity=33.125, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI53729339, Length=222, Percent_Identity=28.8288288288288, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI53729337, Length=222, Percent_Identity=28.8288288288288, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1788922, Length=594, Percent_Identity=59.0909090909091, Blast_Score=719, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=518, Percent_Identity=28.5714285714286, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1789738, Length=162, Percent_Identity=35.1851851851852, Blast_Score=84, Evalue=4e-17,
Organism=Escherichia coli, GI1790835, Length=168, Percent_Identity=31.547619047619, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1789559, Length=224, Percent_Identity=29.4642857142857, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=614, Percent_Identity=39.413680781759, Blast_Score=469, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI71988811, Length=139, Percent_Identity=38.8489208633094, Blast_Score=105, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI71988819, Length=139, Percent_Identity=38.8489208633094, Blast_Score=105, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17556745, Length=464, Percent_Identity=27.1551724137931, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17533571, Length=163, Percent_Identity=34.9693251533742, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=159, Percent_Identity=37.7358490566038, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=33.0827067669173, Blast_Score=87, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71994658, Length=225, Percent_Identity=28.4444444444444, Blast_Score=72, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6323320, Length=605, Percent_Identity=46.7768595041322, Blast_Score=557, Evalue=1e-159,
Organism=Saccharomyces cerevisiae, GI6324707, Length=147, Percent_Identity=41.4965986394558, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320593, Length=147, Percent_Identity=41.4965986394558, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6323098, Length=183, Percent_Identity=34.9726775956284, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324166, Length=171, Percent_Identity=35.672514619883, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6322359, Length=118, Percent_Identity=37.2881355932203, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324761, Length=246, Percent_Identity=28.0487804878049, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI78706572, Length=605, Percent_Identity=45.9504132231405, Blast_Score=564, Evalue=1e-161,
Organism=Drosophila melanogaster, GI28574573, Length=138, Percent_Identity=44.9275362318841, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24582462, Length=163, Percent_Identity=37.4233128834356, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24585711, Length=151, Percent_Identity=37.7483443708609, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24585713, Length=151, Percent_Identity=37.7483443708609, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24585709, Length=151, Percent_Identity=37.7483443708609, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21357743, Length=168, Percent_Identity=34.5238095238095, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI221458488, Length=151, Percent_Identity=39.7350993377483, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 66746; Mature: 66615

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAPIDNIRNFSIVAHIDHGKSTLADRLIQITGGMSDREMAGKEQVLDSMDIERERGIT
CCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHCCHHHCCCE
IKAQTVRLKYRAHDGKDYIFNLMDTPGHVDFAYEVSRSLAACEGSLLVVDASQGVEAQTL
EEEEEEEEEEECCCCCCEEEEECCCCCCEEHHHHHHHHHHHCCCCEEEEECCCCCCHHHH
ANVYHALDAGHEIVPVLNKVDLPAAEPEKIKQQIEDVIGLDASDAVMISAKTGLGVPDVL
HHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHH
EAIVTRLPPPKGDRDATLKALLVDSWYDVYLGVVVLVRVVDGVLKKGQRIRMMGTGAAYD
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
VERVGYFTPKMVNVEELGPGEVGFITAAIKEVADTRVGDTITDDKKPVTDMLPGFKPAIP
HHHHCCCCCCEEEHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCHH
VVFCGLFPVDADDFETLRAAMGKLRLNDASFSFEMETSAALGFGFRCGFLGLLHLEIIQE
HHHHCCCCCCCCHHHHHHHHHCCEEECCCCEEEEECCCHHHCCCHHHHHHHHHHHHHHHH
RLSREFDLDLIATAPSVIYKMKLTDGTEMEIHNPVDMPDVVKIAEIEEPWIEATILTPDE
HHCCCCCCEEEECCCCEEEEEEECCCCCEEECCCCCCCHHEEHHHCCCCCEEEEEECCHH
YLGSVLKLCQDRRGNQKELTYVGARAMVKYDLPLNEVVFDFYDRLKSVSKGYASFDYHLT
HHHHHHHHHHHCCCCCCCEEEECCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEC
DYKPADLVKMQILVNAEPVDALSMLVHRTRAEGRGRAMVEKMKELIPPHMFQIPIQAAIG
CCCCHHHEEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEHHHHCC
GKVIARETVRALRKDVTAKCYGGDITRKRKLLEKQKEGKKKMRQFGKVDIPQEAFIAALK
CHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEE
VDS
ECC
>Mature Secondary Structure 
TTAPIDNIRNFSIVAHIDHGKSTLADRLIQITGGMSDREMAGKEQVLDSMDIERERGIT
CCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHCCHHHCCCE
IKAQTVRLKYRAHDGKDYIFNLMDTPGHVDFAYEVSRSLAACEGSLLVVDASQGVEAQTL
EEEEEEEEEEECCCCCCEEEEECCCCCCEEHHHHHHHHHHHCCCCEEEEECCCCCCHHHH
ANVYHALDAGHEIVPVLNKVDLPAAEPEKIKQQIEDVIGLDASDAVMISAKTGLGVPDVL
HHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHH
EAIVTRLPPPKGDRDATLKALLVDSWYDVYLGVVVLVRVVDGVLKKGQRIRMMGTGAAYD
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
VERVGYFTPKMVNVEELGPGEVGFITAAIKEVADTRVGDTITDDKKPVTDMLPGFKPAIP
HHHHCCCCCCEEEHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCHH
VVFCGLFPVDADDFETLRAAMGKLRLNDASFSFEMETSAALGFGFRCGFLGLLHLEIIQE
HHHHCCCCCCCCHHHHHHHHHCCEEECCCCEEEEECCCHHHCCCHHHHHHHHHHHHHHHH
RLSREFDLDLIATAPSVIYKMKLTDGTEMEIHNPVDMPDVVKIAEIEEPWIEATILTPDE
HHCCCCCCEEEECCCCEEEEEEECCCCCEEECCCCCCCHHEEHHHCCCCCEEEEEECCHH
YLGSVLKLCQDRRGNQKELTYVGARAMVKYDLPLNEVVFDFYDRLKSVSKGYASFDYHLT
HHHHHHHHHHHCCCCCCCEEEECCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEC
DYKPADLVKMQILVNAEPVDALSMLVHRTRAEGRGRAMVEKMKELIPPHMFQIPIQAAIG
CCCCHHHEEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEHHHHCC
GKVIARETVRALRKDVTAKCYGGDITRKRKLLEKQKEGKKKMRQFGKVDIPQEAFIAALK
CHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEE
VDS
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA