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The map label for this gene is hslU [H]

Identifier: 192288739

GI number: 192288739

Start: 329807

End: 331108

Strand: Reverse

Name: hslU [H]

Synonym: Rpal_0308

Alternate gene names: 192288739

Gene position: 331108-329807 (Counterclockwise)

Preceding gene: 192288740

Following gene: 192288735

Centisome position: 5.76

GC content: 64.82

Gene sequence:

>1302_bases
ATGACCGACTTTTCCCCCCGTGAAATCGTCTCCGAACTCGACCGCTTCATCGTCGGCCAGGCCGACGCCAAGCGCGCGGT
CGCCATCGCGCTGCGCAATCGCTGGCGGCGGCTGCAGCTCGAAGGTAGCTTGCGCGAAGAGGTGCTGCCGAAAAACATCC
TGATGATCGGCCCGACCGGCGTCGGCAAAACCGAGATCGCCCGGCGACTCGCCAAGCTCGCCGGCGCACCGTTCCTCAAG
GTCGAGGCGACCAAGTTCACCGAGGTCGGCTATGTCGGCCGCGACGTCGAACAGATCATCCGCGATCTGGTCGAGGTCGC
GATCGCGCAGGTGCGCGAGAAGAAGCGCAAGGACGTGCAGGCGCGCGCCCAGGTCGCCGCCGAGGAACGCGTGCTCGATG
CACTGGTCGGGCCCGGCTCGGGTCCGGCGACGCGCGACTCGTTCCGCAAGAAGCTGCGCGCCGGCGAACTCAACGACAAG
GAAATCGAGATCGAGACCCAGGCCGGTTCCGGCTCGCCGATGTTCGAGATTCCCGGCATGCCGGGCGCGCAGATCGGCGC
GGTCTCGCTCGGCGACATCTTCGGCAAGATGGGCGGCCGCACCAAGAAGCGGCGGCTGACAGTAGCCGACTCCCACGAGA
TCCTGGTCAACGAAGAGGCCGACAAGCTGCTCGACACTGATCAGCTGGTGCAGGAAGCGATCGCTGCGGTGGAGAACAAC
GGCATCGTGTTCCTGGACGAGATCGACAAGATCTGCGTGCGCGACGGCCGCAGCGGCGGCGAAGTCTCGCGCGAAGGCGT
GCAGCGCGATCTGTTGCCGCTGATCGAAGGCACCACGGTGTCGACCAAGCACGGGGCAGTGAAGACCGAGCACATCCTGT
TCATCGCTTCGGGCGCGTTCCACATCGCCAAGCCCTCCGACCTGCTGCCCGAGCTGCAGGGACGTCTGCCGATCCGTGTC
GAACTCAATGCGCTCAGCCGAGACGACATGCGCCGCATTCTGACCGAGCCGGAAGCGTCGTTGATCAAGCAATATGTGGC
GCTGATGAAAACCGAGGGCGTGACGCTGGACTTCTCCGACGACGCCATCGACGCACTGGCCGATGTGGCGGTGGCGGTGA
ACTCCACCGTCGAGAACATCGGCGCGCGCCGACTGCAGACCGTGATGGAGCGCGTCCTCGACGAGATCTCGTTCGTCGCC
CCAGACCGCCACGGCGAGACCTTCCAGGTCGATGCAGACTACGTGAAGACCAATGTCGGGGACCTGGCCAAGAACACCGA
TCTGAGCCGGTTTATTCTGTAA

Upstream 100 bases:

>100_bases
TGCTGATTCATCCGTTCGATGCCATCCGGCAATGGCAGGCGGGACCTCCGATCATCTGACCCCGCTTGTTCCCTCGTTCG
ACCGACCTTACGTAGGCCCC

Downstream 100 bases:

>100_bases
CCGGCCGTTTGGGGCAGCTCTGTCGGCTCTGACGACGAAAGAACAGGGCTCATCCTCAAGTACCAACGTCATGCGCGGGC
TCGACCCGCGCATCCATCCT

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MTDFSPREIVSELDRFIVGQADAKRAVAIALRNRWRRLQLEGSLREEVLPKNILMIGPTGVGKTEIARRLAKLAGAPFLK
VEATKFTEVGYVGRDVEQIIRDLVEVAIAQVREKKRKDVQARAQVAAEERVLDALVGPGSGPATRDSFRKKLRAGELNDK
EIEIETQAGSGSPMFEIPGMPGAQIGAVSLGDIFGKMGGRTKKRRLTVADSHEILVNEEADKLLDTDQLVQEAIAAVENN
GIVFLDEIDKICVRDGRSGGEVSREGVQRDLLPLIEGTTVSTKHGAVKTEHILFIASGAFHIAKPSDLLPELQGRLPIRV
ELNALSRDDMRRILTEPEASLIKQYVALMKTEGVTLDFSDDAIDALADVAVAVNSTVENIGARRLQTVMERVLDEISFVA
PDRHGETFQVDADYVKTNVGDLAKNTDLSRFIL

Sequences:

>Translated_433_residues
MTDFSPREIVSELDRFIVGQADAKRAVAIALRNRWRRLQLEGSLREEVLPKNILMIGPTGVGKTEIARRLAKLAGAPFLK
VEATKFTEVGYVGRDVEQIIRDLVEVAIAQVREKKRKDVQARAQVAAEERVLDALVGPGSGPATRDSFRKKLRAGELNDK
EIEIETQAGSGSPMFEIPGMPGAQIGAVSLGDIFGKMGGRTKKRRLTVADSHEILVNEEADKLLDTDQLVQEAIAAVENN
GIVFLDEIDKICVRDGRSGGEVSREGVQRDLLPLIEGTTVSTKHGAVKTEHILFIASGAFHIAKPSDLLPELQGRLPIRV
ELNALSRDDMRRILTEPEASLIKQYVALMKTEGVTLDFSDDAIDALADVAVAVNSTVENIGARRLQTVMERVLDEISFVA
PDRHGETFQVDADYVKTNVGDLAKNTDLSRFIL
>Mature_432_residues
TDFSPREIVSELDRFIVGQADAKRAVAIALRNRWRRLQLEGSLREEVLPKNILMIGPTGVGKTEIARRLAKLAGAPFLKV
EATKFTEVGYVGRDVEQIIRDLVEVAIAQVREKKRKDVQARAQVAAEERVLDALVGPGSGPATRDSFRKKLRAGELNDKE
IEIETQAGSGSPMFEIPGMPGAQIGAVSLGDIFGKMGGRTKKRRLTVADSHEILVNEEADKLLDTDQLVQEAIAAVENNG
IVFLDEIDKICVRDGRSGGEVSREGVQRDLLPLIEGTTVSTKHGAVKTEHILFIASGAFHIAKPSDLLPELQGRLPIRVE
LNALSRDDMRRILTEPEASLIKQYVALMKTEGVTLDFSDDAIDALADVAVAVNSTVENIGARRLQTVMERVLDEISFVAP
DRHGETFQVDADYVKTNVGDLAKNTDLSRFIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily [H]

Homologues:

Organism=Homo sapiens, GI7242140, Length=109, Percent_Identity=34.8623853211009, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1790366, Length=446, Percent_Identity=59.4170403587444, Blast_Score=511, Evalue=1e-146,
Organism=Escherichia coli, GI1786642, Length=113, Percent_Identity=43.3628318584071, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6319704, Length=108, Percent_Identity=37.037037037037, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24648289, Length=257, Percent_Identity=29.9610894941634, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24648291, Length=257, Percent_Identity=29.9610894941634, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 47461; Mature: 47330

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDFSPREIVSELDRFIVGQADAKRAVAIALRNRWRRLQLEGSLREEVLPKNILMIGPTG
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEECCCHHHHHCCCCEEEECCCC
VGKTEIARRLAKLAGAPFLKVEATKFTEVGYVGRDVEQIIRDLVEVAIAQVREKKRKDVQ
CCHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARAQVAAEERVLDALVGPGSGPATRDSFRKKLRAGELNDKEIEIETQAGSGSPMFEIPGM
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEECCCC
PGAQIGAVSLGDIFGKMGGRTKKRRLTVADSHEILVNEEADKLLDTDQLVQEAIAAVENN
CCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCEEEECCHHHHCCHHHHHHHHHHHHCCC
GIVFLDEIDKICVRDGRSGGEVSREGVQRDLLPLIEGTTVSTKHGAVKTEHILFIASGAF
CEEEEECHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEEEEECCCE
HIAKPSDLLPELQGRLPIRVELNALSRDDMRRILTEPEASLIKQYVALMKTEGVTLDFSD
EECCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEECCC
DAIDALADVAVAVNSTVENIGARRLQTVMERVLDEISFVAPDRHGETFQVDADYVKTNVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHCCHH
DLAKNTDLSRFIL
HHHCCCCHHHHCC
>Mature Secondary Structure 
TDFSPREIVSELDRFIVGQADAKRAVAIALRNRWRRLQLEGSLREEVLPKNILMIGPTG
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEECCCHHHHHCCCCEEEECCCC
VGKTEIARRLAKLAGAPFLKVEATKFTEVGYVGRDVEQIIRDLVEVAIAQVREKKRKDVQ
CCHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARAQVAAEERVLDALVGPGSGPATRDSFRKKLRAGELNDKEIEIETQAGSGSPMFEIPGM
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEECCCC
PGAQIGAVSLGDIFGKMGGRTKKRRLTVADSHEILVNEEADKLLDTDQLVQEAIAAVENN
CCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCEEEECCHHHHCCHHHHHHHHHHHHCCC
GIVFLDEIDKICVRDGRSGGEVSREGVQRDLLPLIEGTTVSTKHGAVKTEHILFIASGAF
CEEEEECHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEEEEECCCE
HIAKPSDLLPELQGRLPIRVELNALSRDDMRRILTEPEASLIKQYVALMKTEGVTLDFSD
EECCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEECCC
DAIDALADVAVAVNSTVENIGARRLQTVMERVLDEISFVAPDRHGETFQVDADYVKTNVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHCCHH
DLAKNTDLSRFIL
HHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA