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The map label for this gene is priA [H]

Identifier: 192288609

GI number: 192288609

Start: 191082

End: 193292

Strand: Reverse

Name: priA [H]

Synonym: Rpal_0176

Alternate gene names: 192288609

Gene position: 193292-191082 (Counterclockwise)

Preceding gene: 192288612

Following gene: 192288607

Centisome position: 3.37

GC content: 67.44

Gene sequence:

>2211_bases
ATGGATGATTTGCCGACCAGCAGCCCGGCCTCCCACGCGCCTGCCAGCGTCGTCGACGTGCTGGTGCCGGTCGCGATCGA
TCAGGCCTATTCGTACCGGGTTCCGCGCGGCCTTTCGCTGAAACCCGGAGACGTCGTGGCTGTGCCGCTGGGCGCTCGTG
AAGTGCTCGGCGTGGTCTGGGCGGAGAATCCGAATCCCGACCCGCGGCTGCACAATCGCCTCAAGGATGTGGCCGAAAAG
CTCGACGTACCGCCGCTGCGCGACGAACTCCGCCGGATGGTCGACTGGGTTTCGACCTACACGCTGTCGGCGCGCGGGAT
GGTGCTGCGGATGTGCTTGCGGATGGGCGAGCATCTCGGCCCGGAGCGGCAGCGCATGGGGGTGCGGCTGGTGGGCTCGC
CGCCGCAGCGGATGACGCCAGCGCGCCGCCGCCTGCTCCAGATCCTGTCGGACGGGCTGCTGCACGGCAAATCCGACGTC
GCCAAGGAAGCCGGCGTCAGCCCCGGCGTGATCGACGGCCTGGTCGACGAGGGCACCCTGCAGGTCGAGCAGATGCCGCG
CGAAAACCCGGCGCCGGAGCCCGATCCGGATTTCGCGCCGGCCGACTTTTCGCCCGAGCAGGCCCGTGCGGCCGAGACGA
TGCGCGGCCTTGTGACCGCCGGCGGCTTTCAGGCGGCGCTGATCGACGGCGTCACCGGCTCCGGCAAGACCGAAGTGTAT
TTCGAGGCGGTCGCCGAGGTCATTCGGCAGGGCCGGCAGAGCCTGATCCTGATGCCGGAGATCGCGCTGACCGGGCAGTT
TCTCGACCGCTTCGCACAGCGATTCGGTGTGCGGCCGCTGGAATGGCATTCCGAACTGACCCCGCGCACCCGCGCGCGCA
ACTGGGCTTCGATCGCCGCCGGCGAAGCCCGCGTCGTGGTCGGCGCGCGCTCGGCGCTGTTTCTGCCTTACGCCGATCTC
GGGCTGATCATCGTCGATGAAGAGCACGACCAGGCCTACAAGCAGGAGGACGGCGCGCATTATCACGCCCGCGACATGGC
GGTGGTGCGGGCTTCGATCGCGAAAATTCCGATCGTGCTGGCGTCGGCGACGCCATCGGTCGAGACCGAGGTCAATGCCC
GTAAGGGGCGCTACACGCGGATTCCGCTGCCGTCACGATTCGGCGGTCAGCACATGCCGCAGATCGAAGCGATCGATCTG
CGGCGCGAGCCGCCGATGCGCGGCCGCTTCATTTCGCCGCGGCTCGCCGAGCAGATCGGTTTTGCGATCGAGCGGCGTGA
ACAGGCGCTGCTGTTTCTCAATCGCCGCGGCTATGCGCCGCTGACGCTGTGCCGCGCCTGCGGCCATCGCTTTGCCTGCA
CCATCTGCGACGCCTGGCTGGTCGATCACCGCTTCCGGCAGCGCCTGGTGTGCCACCATTGCGGGTTCTCGATGCCGCGG
CCGCCAGCCTGTCCGCATTGCGGCGCCGAGGGCTCGCTGGTCGCGGTCGGGCCCGGCGTCGAACGGCTGCAGGAAGAGGC
GGCGAACCTGTTTCCCAATGCCCGCACCATGGTGCTGTCGAGCGATCTGATTACGTCGATCGAGACGATGCGCGCCGAGC
TGAACGAGATAGCGGAGGGCCGGGTCGACATCATCATCGGTACCCAGCTCGTCGCCAAGGGCCATAATTTCCCGCGGCTC
AATCTGGTCGGCGTGGTCGATGCCGACCTCGGCCTGTCCAACGGCGATCCGCGCGCCGCCGAGCGCACCTTCCAGCTGCT
CAATCAGGTGATCGGCCGTGCCGGCCGCGATCAGGGCCGCGGCGTTGGCTTCCTGCAGACCCATCAGCCCGAGCATCCGG
TGATGAAGGCGCTGGTCGCCTGTGACCGCGAGGCGTTTTACGCCAGTGAAATCGATATCCGCGAGCGCACGCTGTATCCG
CCGTTTGGCCGGCTTGCGAGCCTGATCATCTCGGCCGGCGACCGGCCGACCGCAGAAGGCTTCGGCCGCCAGCTCGCCAG
TGCCGCGCCGTTCGATGAGCGCGTGCAAGTGCTGGGCCCGGCCGAAGCGCCGCTCGCGGTGATCAAGGGGCGCTATCGAT
TCCGGCTGCTGGTGAAATCGGTGCGCGGCTTCGACCTGTCGAACTACTTACGCTCTTGGCTGGCCAAAGGACCCAAGACA
AAAGGTAATCTCAAGCTCGAGGTCGACGTCGATCCGCAAAGCTTTCTGTGA

Upstream 100 bases:

>100_bases
TCGTCGCTTTGCTCCTCGCAACGACGCGGAGAGGATGCGGCAATCATAGCTGATTTCCTTTCCCATTGATGAAACCGGGC
TTTGGCGGCACACCGGGCCG

Downstream 100 bases:

>100_bases
TCTTGCGGCTCGGCTGACGCGCAAAAGAAAAGCCTGCCGCTACAATCGGCGGCAGGCTTTTGTCACGTGACGTTAGAGCG
GAGTGCGGCTTGTTGCCGAA

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 736; Mature: 736

Protein sequence:

>736_residues
MDDLPTSSPASHAPASVVDVLVPVAIDQAYSYRVPRGLSLKPGDVVAVPLGAREVLGVVWAENPNPDPRLHNRLKDVAEK
LDVPPLRDELRRMVDWVSTYTLSARGMVLRMCLRMGEHLGPERQRMGVRLVGSPPQRMTPARRRLLQILSDGLLHGKSDV
AKEAGVSPGVIDGLVDEGTLQVEQMPRENPAPEPDPDFAPADFSPEQARAAETMRGLVTAGGFQAALIDGVTGSGKTEVY
FEAVAEVIRQGRQSLILMPEIALTGQFLDRFAQRFGVRPLEWHSELTPRTRARNWASIAAGEARVVVGARSALFLPYADL
GLIIVDEEHDQAYKQEDGAHYHARDMAVVRASIAKIPIVLASATPSVETEVNARKGRYTRIPLPSRFGGQHMPQIEAIDL
RREPPMRGRFISPRLAEQIGFAIERREQALLFLNRRGYAPLTLCRACGHRFACTICDAWLVDHRFRQRLVCHHCGFSMPR
PPACPHCGAEGSLVAVGPGVERLQEEAANLFPNARTMVLSSDLITSIETMRAELNEIAEGRVDIIIGTQLVAKGHNFPRL
NLVGVVDADLGLSNGDPRAAERTFQLLNQVIGRAGRDQGRGVGFLQTHQPEHPVMKALVACDREAFYASEIDIRERTLYP
PFGRLASLIISAGDRPTAEGFGRQLASAAPFDERVQVLGPAEAPLAVIKGRYRFRLLVKSVRGFDLSNYLRSWLAKGPKT
KGNLKLEVDVDPQSFL

Sequences:

>Translated_736_residues
MDDLPTSSPASHAPASVVDVLVPVAIDQAYSYRVPRGLSLKPGDVVAVPLGAREVLGVVWAENPNPDPRLHNRLKDVAEK
LDVPPLRDELRRMVDWVSTYTLSARGMVLRMCLRMGEHLGPERQRMGVRLVGSPPQRMTPARRRLLQILSDGLLHGKSDV
AKEAGVSPGVIDGLVDEGTLQVEQMPRENPAPEPDPDFAPADFSPEQARAAETMRGLVTAGGFQAALIDGVTGSGKTEVY
FEAVAEVIRQGRQSLILMPEIALTGQFLDRFAQRFGVRPLEWHSELTPRTRARNWASIAAGEARVVVGARSALFLPYADL
GLIIVDEEHDQAYKQEDGAHYHARDMAVVRASIAKIPIVLASATPSVETEVNARKGRYTRIPLPSRFGGQHMPQIEAIDL
RREPPMRGRFISPRLAEQIGFAIERREQALLFLNRRGYAPLTLCRACGHRFACTICDAWLVDHRFRQRLVCHHCGFSMPR
PPACPHCGAEGSLVAVGPGVERLQEEAANLFPNARTMVLSSDLITSIETMRAELNEIAEGRVDIIIGTQLVAKGHNFPRL
NLVGVVDADLGLSNGDPRAAERTFQLLNQVIGRAGRDQGRGVGFLQTHQPEHPVMKALVACDREAFYASEIDIRERTLYP
PFGRLASLIISAGDRPTAEGFGRQLASAAPFDERVQVLGPAEAPLAVIKGRYRFRLLVKSVRGFDLSNYLRSWLAKGPKT
KGNLKLEVDVDPQSFL
>Mature_736_residues
MDDLPTSSPASHAPASVVDVLVPVAIDQAYSYRVPRGLSLKPGDVVAVPLGAREVLGVVWAENPNPDPRLHNRLKDVAEK
LDVPPLRDELRRMVDWVSTYTLSARGMVLRMCLRMGEHLGPERQRMGVRLVGSPPQRMTPARRRLLQILSDGLLHGKSDV
AKEAGVSPGVIDGLVDEGTLQVEQMPRENPAPEPDPDFAPADFSPEQARAAETMRGLVTAGGFQAALIDGVTGSGKTEVY
FEAVAEVIRQGRQSLILMPEIALTGQFLDRFAQRFGVRPLEWHSELTPRTRARNWASIAAGEARVVVGARSALFLPYADL
GLIIVDEEHDQAYKQEDGAHYHARDMAVVRASIAKIPIVLASATPSVETEVNARKGRYTRIPLPSRFGGQHMPQIEAIDL
RREPPMRGRFISPRLAEQIGFAIERREQALLFLNRRGYAPLTLCRACGHRFACTICDAWLVDHRFRQRLVCHHCGFSMPR
PPACPHCGAEGSLVAVGPGVERLQEEAANLFPNARTMVLSSDLITSIETMRAELNEIAEGRVDIIIGTQLVAKGHNFPRL
NLVGVVDADLGLSNGDPRAAERTFQLLNQVIGRAGRDQGRGVGFLQTHQPEHPVMKALVACDREAFYASEIDIRERTLYP
PFGRLASLIISAGDRPTAEGFGRQLASAAPFDERVQVLGPAEAPLAVIKGRYRFRLLVKSVRGFDLSNYLRSWLAKGPKT
KGNLKLEVDVDPQSFL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=748, Percent_Identity=34.6256684491979, Blast_Score=365, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD [H]

EC number: NA

Molecular weight: Translated: 80942; Mature: 80942

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDLPTSSPASHAPASVVDVLVPVAIDQAYSYRVPRGLSLKPGDVVAVPLGAREVLGVVW
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHEEEE
AENPNPDPRLHNRLKDVAEKLDVPPLRDELRRMVDWVSTYTLSARGMVLRMCLRMGEHLG
CCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
PERQRMGVRLVGSPPQRMTPARRRLLQILSDGLLHGKSDVAKEAGVSPGVIDGLVDEGTL
CHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHCCCCE
QVEQMPRENPAPEPDPDFAPADFSPEQARAAETMRGLVTAGGFQAALIDGVTGSGKTEVY
EHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHH
FEAVAEVIRQGRQSLILMPEIALTGQFLDRFAQRFGVRPLEWHSELTPRTRARNWASIAA
HHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHCCCCHHHCC
GEARVVVGARSALFLPYADLGLIIVDEEHDQAYKQEDGAHYHARDMAVVRASIAKIPIVL
CCEEEEEECCCEEEECCCCCCEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCEEE
ASATPSVETEVNARKGRYTRIPLPSRFGGQHMPQIEAIDLRREPPMRGRFISPRLAEQIG
ECCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHH
FAIERREQALLFLNRRGYAPLTLCRACGHRFACTICDAWLVDHRFRQRLVCHHCGFSMPR
HHHHHHHHEEEEEECCCCCHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PPACPHCGAEGSLVAVGPGVERLQEEAANLFPNARTMVLSSDLITSIETMRAELNEIAEG
CCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHCC
RVDIIIGTQLVAKGHNFPRLNLVGVVDADLGLSNGDPRAAERTFQLLNQVIGRAGRDQGR
CEEEEECCHHHHCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
GVGFLQTHQPEHPVMKALVACDREAFYASEIDIRERTLYPPFGRLASLIISAGDRPTAEG
CCCEECCCCCCHHHHHHHHHHCCHHHHHCCCCHHHCCCCCCHHHHHHHHHHCCCCCCHHH
FGRQLASAAPFDERVQVLGPAEAPLAVIKGRYRFRLLVKSVRGFDLSNYLRSWLAKGPKT
HHHHHHHCCCHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
KGNLKLEVDVDPQSFL
CCCEEEEEECCHHHCC
>Mature Secondary Structure
MDDLPTSSPASHAPASVVDVLVPVAIDQAYSYRVPRGLSLKPGDVVAVPLGAREVLGVVW
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHEEEE
AENPNPDPRLHNRLKDVAEKLDVPPLRDELRRMVDWVSTYTLSARGMVLRMCLRMGEHLG
CCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
PERQRMGVRLVGSPPQRMTPARRRLLQILSDGLLHGKSDVAKEAGVSPGVIDGLVDEGTL
CHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHCCCCE
QVEQMPRENPAPEPDPDFAPADFSPEQARAAETMRGLVTAGGFQAALIDGVTGSGKTEVY
EHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHH
FEAVAEVIRQGRQSLILMPEIALTGQFLDRFAQRFGVRPLEWHSELTPRTRARNWASIAA
HHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHCCCCHHHCC
GEARVVVGARSALFLPYADLGLIIVDEEHDQAYKQEDGAHYHARDMAVVRASIAKIPIVL
CCEEEEEECCCEEEECCCCCCEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCEEE
ASATPSVETEVNARKGRYTRIPLPSRFGGQHMPQIEAIDLRREPPMRGRFISPRLAEQIG
ECCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHH
FAIERREQALLFLNRRGYAPLTLCRACGHRFACTICDAWLVDHRFRQRLVCHHCGFSMPR
HHHHHHHHEEEEEECCCCCHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PPACPHCGAEGSLVAVGPGVERLQEEAANLFPNARTMVLSSDLITSIETMRAELNEIAEG
CCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHCC
RVDIIIGTQLVAKGHNFPRLNLVGVVDADLGLSNGDPRAAERTFQLLNQVIGRAGRDQGR
CEEEEECCHHHHCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
GVGFLQTHQPEHPVMKALVACDREAFYASEIDIRERTLYPPFGRLASLIISAGDRPTAEG
CCCEECCCCCCHHHHHHHHHHCCHHHHHCCCCHHHCCCCCCHHHHHHHHHHCCCCCCHHH
FGRQLASAAPFDERVQVLGPAEAPLAVIKGRYRFRLLVKSVRGFDLSNYLRSWLAKGPKT
HHHHHHHCCCHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
KGNLKLEVDVDPQSFL
CCCEEEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2861810 [H]