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The map label for this gene is dut

Identifier: 192288516

GI number: 192288516

Start: 92174

End: 92632

Strand: Reverse

Name: dut

Synonym: Rpal_0083

Alternate gene names: 192288516

Gene position: 92632-92174 (Counterclockwise)

Preceding gene: 192288517

Following gene: 192288515

Centisome position: 1.61

GC content: 69.06

Gene sequence:

>459_bases
ATGACCCAAAAGATCACCGTTTCGATTCGCCATCTGCCGCACGGCGAAGGCTTGCCGCTGCCGGAGTATCAGACCGCGCA
CGCCGCCGGGCTCGACCTGATCGCCGCCGTCCCGCAGGATGCGCCGCTGACGCTTCAGCCCGGCCGCTACGTGCTGGTGC
CGACCGGGCTCACCATCGCGCTTCCGGAGAACTACGAGGCGCAGGTGCGGCCGCGTTCGGGCCTCGCCGCCAAGCACGGT
GTCACCGTGCTGAACGCGCCGGGCACGATCGACGCCGATTATCGCGGTGAGATCGGCGTGCTGCTGATTAACCACGGTAC
CGAACCGTTCGCGATCCGCCGCGGCGAACGCATCGCTCAAATGGTGATCGCCCCGGTCAGCCGGGCTCAGTTCGTGGCGG
TCGAGGCGCTGCCGGAAAGCGGCCGTGGCGCCGGCGGTTTCGGCTCGACCGGCCGCTGA

Upstream 100 bases:

>100_bases
ACGATGCCGCCACCGATCTCGAATCGTGGCCGCTGATGACCAAGGATGAAGTCGCGACCGCGCTGGTGGCGCGGATCGCT
CAAGCAATGGCAGTTCACGC

Downstream 100 bases:

>100_bases
GTAGCGGCGTTGCGTTGAGGTGTTACTTCCGCGACTCAACTAGATTTAGTGTCAAGCACGGCGGGAATCGGCTCGGAGAA
ATATAAATCGTCCGTTTGCG

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 152; Mature: 151

Protein sequence:

>152_residues
MTQKITVSIRHLPHGEGLPLPEYQTAHAAGLDLIAAVPQDAPLTLQPGRYVLVPTGLTIALPENYEAQVRPRSGLAAKHG
VTVLNAPGTIDADYRGEIGVLLINHGTEPFAIRRGERIAQMVIAPVSRAQFVAVEALPESGRGAGGFGSTGR

Sequences:

>Translated_152_residues
MTQKITVSIRHLPHGEGLPLPEYQTAHAAGLDLIAAVPQDAPLTLQPGRYVLVPTGLTIALPENYEAQVRPRSGLAAKHG
VTVLNAPGTIDADYRGEIGVLLINHGTEPFAIRRGERIAQMVIAPVSRAQFVAVEALPESGRGAGGFGSTGR
>Mature_151_residues
TQKITVSIRHLPHGEGLPLPEYQTAHAAGLDLIAAVPQDAPLTLQPGRYVLVPTGLTIALPENYEAQVRPRSGLAAKHGV
TVLNAPGTIDADYRGEIGVLLINHGTEPFAIRRGERIAQMVIAPVSRAQFVAVEALPESGRGAGGFGSTGR

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=132, Percent_Identity=43.9393939393939, Blast_Score=106, Evalue=8e-24,
Organism=Homo sapiens, GI4503423, Length=132, Percent_Identity=43.9393939393939, Blast_Score=105, Evalue=1e-23,
Organism=Homo sapiens, GI70906441, Length=132, Percent_Identity=43.9393939393939, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1790071, Length=151, Percent_Identity=45.6953642384106, Blast_Score=120, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI71988561, Length=152, Percent_Identity=43.421052631579, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319729, Length=134, Percent_Identity=41.044776119403, Blast_Score=100, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24583610, Length=147, Percent_Identity=38.0952380952381, Blast_Score=92, Evalue=9e-20,
Organism=Drosophila melanogaster, GI19921126, Length=147, Percent_Identity=38.0952380952381, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_RHOPA (P61911)

Other databases:

- EMBL:   BX572593
- RefSeq:   NP_945436.1
- ProteinModelPortal:   P61911
- GeneID:   2689183
- GenomeReviews:   BX571963_GR
- KEGG:   rpa:RPA0080
- NMPDR:   fig|258594.1.peg.80
- HOGENOM:   HBG436079
- OMA:   HGIALVN
- ProtClustDB:   PRK00601
- BioCyc:   RPAL258594:RPA0080-MONOMER
- BRENDA:   3.6.1.23
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 16002; Mature: 15870

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: NA

Important sites: BINDING 85-85

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQKITVSIRHLPHGEGLPLPEYQTAHAAGLDLIAAVPQDAPLTLQPGRYVLVPTGLTIA
CCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEECCCCEEEEECCEEEE
LPENYEAQVRPRSGLAAKHGVTVLNAPGTIDADYRGEIGVLLINHGTEPFAIRRGERIAQ
ECCCCCEEECCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCEEEECCHHHHH
MVIAPVSRAQFVAVEALPESGRGAGGFGSTGR
HHHCCCCCCEEEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TQKITVSIRHLPHGEGLPLPEYQTAHAAGLDLIAAVPQDAPLTLQPGRYVLVPTGLTIA
CCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEECCCCEEEEECCEEEE
LPENYEAQVRPRSGLAAKHGVTVLNAPGTIDADYRGEIGVLLINHGTEPFAIRRGERIAQ
ECCCCCEEECCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCEEEECCHHHHH
MVIAPVSRAQFVAVEALPESGRGAGGFGSTGR
HHHCCCCCCEEEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA