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The map label for this gene is opuAB [H]

Identifier: 190894908

GI number: 190894908

Start: 626616

End: 627467

Strand: Reverse

Name: opuAB [H]

Synonym: RHECIAT_PC0000573

Alternate gene names: 190894908

Gene position: 627467-626616 (Counterclockwise)

Preceding gene: 190894909

Following gene: 190894907

Centisome position: 57.49

GC content: 64.44

Gene sequence:

>852_bases
ATGAATATTTCGGACTTGCAGTTTTCGCCCGGCGCTTTCCTGGCCCCGGCCGTCGATTGGCTCAACACCAACCTTCATCC
GCTGTTTGCGGCCATCAGCTATGTGGTCGAAACGGTGCTTTCCGCCATCGAGGCCGCACTTCTCTTCGTGCCGCCTTTTG
CGCTGATCGCGATCGTCGTCGCGGCGGCATTCTTTGCCGTCAGCCTGCGCGCCGCCATCGTCGCCGGCCTCTGCCTCGGC
TTCTGCCTGCTTGTCGGACTGTGGACGGCGTCGATGCAGACGCTTGCGCTGGTCACCGTCGCCGTCGTGATCTCCGTTCT
CATCGCCTTTCCGATCGGCGTACTCTGTTCGCGCTATAAGACATTGGAGGCGGCGGTTCGCCCGGTTCTCGACGTGATGC
AGACCGTGCCGCCATGGGTCTATCTCATTCCGGCGGTGATGATCTTCAGCCTCGGACGCGTGCCGGCGATCATCGCCACC
ATCGTCTATGGCATTCCGCCGATGCTGCGTCTGACGACGCTTGCCTTCAACCAGGTGCCGAAGGTGTTCATCGAGCTCGG
CAGCGCCATCGGCGCACCGCCCCGCTCGATCCTGTGGAAGATCGAGATGCCGCTTGCCAAGCAGACGCTGCTGGTCGGGC
TCAACCAATGCATCCTGCTGTCGCTGGCGATGGTTGTGCTCGCCGGTCTCGTGGGTGCGGGCGGGCTCGGCGCGGAAGTG
ACGCGCGGCTTGACGCGCATGGAAATGGGGCTCGGCCTCAGAGCAGGCCTGGCAATCGTTGCCGTCGCCCTGCTGCTCGA
CCGGTTGACCCGCGGCCTGTTCGACCGCGGCCGGCTGGCGAAGGCGCGGTGA

Upstream 100 bases:

>100_bases
TCGCGACTTCTATCAATGCGGCGATCGCCATCGTGGTATTGACCATGGTGATTGACCGAATAACGCAGAGCGCCGCTCGC
TTGGGAACGGGGAGGAAGTC

Downstream 100 bases:

>100_bases
GCGGGGATATGAAAAAATGAGAAACAGCTTCTTCTGCATCGACGCCCATACCTGCGGCAACCCGGTCCGTCTCGTCGCCG
GCGGCGGTCCGCTGCTTCCG

Product: putative glycine betaine/L-proline ABC transporter permease

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MNISDLQFSPGAFLAPAVDWLNTNLHPLFAAISYVVETVLSAIEAALLFVPPFALIAIVVAAAFFAVSLRAAIVAGLCLG
FCLLVGLWTASMQTLALVTVAVVISVLIAFPIGVLCSRYKTLEAAVRPVLDVMQTVPPWVYLIPAVMIFSLGRVPAIIAT
IVYGIPPMLRLTTLAFNQVPKVFIELGSAIGAPPRSILWKIEMPLAKQTLLVGLNQCILLSLAMVVLAGLVGAGGLGAEV
TRGLTRMEMGLGLRAGLAIVAVALLLDRLTRGLFDRGRLAKAR

Sequences:

>Translated_283_residues
MNISDLQFSPGAFLAPAVDWLNTNLHPLFAAISYVVETVLSAIEAALLFVPPFALIAIVVAAAFFAVSLRAAIVAGLCLG
FCLLVGLWTASMQTLALVTVAVVISVLIAFPIGVLCSRYKTLEAAVRPVLDVMQTVPPWVYLIPAVMIFSLGRVPAIIAT
IVYGIPPMLRLTTLAFNQVPKVFIELGSAIGAPPRSILWKIEMPLAKQTLLVGLNQCILLSLAMVVLAGLVGAGGLGAEV
TRGLTRMEMGLGLRAGLAIVAVALLLDRLTRGLFDRGRLAKAR
>Mature_283_residues
MNISDLQFSPGAFLAPAVDWLNTNLHPLFAAISYVVETVLSAIEAALLFVPPFALIAIVVAAAFFAVSLRAAIVAGLCLG
FCLLVGLWTASMQTLALVTVAVVISVLIAFPIGVLCSRYKTLEAAVRPVLDVMQTVPPWVYLIPAVMIFSLGRVPAIIAT
IVYGIPPMLRLTTLAFNQVPKVFIELGSAIGAPPRSILWKIEMPLAKQTLLVGLNQCILLSLAMVVLAGLVGAGGLGAEV
TRGLTRMEMGLGLRAGLAIVAVALLLDRLTRGLFDRGRLAKAR

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=280, Percent_Identity=38.5714285714286, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1788451, Length=202, Percent_Identity=29.2079207920792, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788449, Length=184, Percent_Identity=28.804347826087, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29938; Mature: 29938

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNISDLQFSPGAFLAPAVDWLNTNLHPLFAAISYVVETVLSAIEAALLFVPPFALIAIVV
CCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
AAAFFAVSLRAAIVAGLCLGFCLLVGLWTASMQTLALVTVAVVISVLIAFPIGVLCSRYK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLEAAVRPVLDVMQTVPPWVYLIPAVMIFSLGRVPAIIATIVYGIPPMLRLTTLAFNQVP
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
KVFIELGSAIGAPPRSILWKIEMPLAKQTLLVGLNQCILLSLAMVVLAGLVGAGGLGAEV
HHHHHHHHHHCCCHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
TRGLTRMEMGLGLRAGLAIVAVALLLDRLTRGLFDRGRLAKAR
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]