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The map label for this gene is pucI [H]

Identifier: 190894814

GI number: 190894814

Start: 516826

End: 518277

Strand: Reverse

Name: pucI [H]

Synonym: RHECIAT_PC0000479

Alternate gene names: 190894814

Gene position: 518277-516826 (Counterclockwise)

Preceding gene: 190894815

Following gene: 190894812

Centisome position: 47.48

GC content: 59.71

Gene sequence:

>1452_bases
ATGAGCATTCGAGATCCGTCTCCCTCGTTGTATAACGAGGACCTTGCACCTGCCGCCGAGCGCAAATGGGGTGCATTCAG
CATTTTCAACGTCTGGACGTCTGACGTCCACAGCCTGTGGGGCTATTATCTGGCGGCGAGCCTGTTTCTGCTGTGCGGCA
GCTTCGTCAATTTCGTCATCGCCATCGGCATCGGTTCCCTGGTCATCTTCATCCTGATGAGTCTGGTCGGCAATGCCGGC
GTGCGCACCGGCGTACCATTTCCGGTGCTGGCCCGTGCCTCCTTCGGCACCTTCGGCGCCAACGTTCCGGCACTGGTCCG
CGCGGTGGTCGCCTGCTTCTGGTACGGGGCGCAGACGGCCGCGGCATCCGGTGCGATCGTTGCCTTGCTGATCAGGAATG
AGAGCCTGCTCGCCTTCCATCAGAACAGTCACATGCTCGGCCACTCGACCCTCGAACTGATCTGCTACGTCATCGTCTGG
GCGCTGCAGCTGCTGATCATTCAGCGCGGCATGGAAACGGTCCGCAAGTTCCAGGATTGGGCCGGCCCGGCCGTGTGGAT
CATGATGCTGATCCTGGCGGTTTATCTGGTTATCAAATCAGGCACTTTCTCATTCGGCGCGGAAATTCCGCGCGACGTGC
TGATCGAAAAAACCAAGGATGCGGGCGTGCCGGGTGAACCGGGCTCGATTGCGGCACTTGCCGCCGTGGCCGCCACCTGG
ATCACCTATTTTGCCGCGCTCTACCTGAACTTCTGCGATTTCTCTCGTTATGCGACGAGTGAAAAAGCGCTCAGGAAAGG
CAATCTCTGGGGCCTGCCGATCAATCTTCTGGCCTTCTGCCTCGTTGCAGGTGTCACGACCACGGCCGCCTTCACCGTCT
ATGGCGAGGTGTTGCTGCATCCGGAAATGATCTCGGCGAAATTCGATAGCTGGTTCCTGGCACTGCTTGCAGCACTGACA
TTTGCGGTCGCGACGCTCGGCATCAACGTCGTGGCCAACTTCGTGTCGCCGGCCTTCGATTTCGCCAATGTCTTCCCGCG
CCAGATCAATTTCAAACGCGGGGGATATGTCGCCGCGCTGATCGCCCTCGTACTCTATCCATTCGCTCCCTGGGAGACGG
GTGCGGCGCATTTCGTCAACTTCATCGGGTCGACGATGGGGCCGATCTTCGGCATCATGATGGTGGACTACTACCTCATC
CGGAAGAGCCAGCTGAACGTTGAAGCGCTCTACCGCGAGAATGGCGAGTTCCGCTTCCAGAACGGCTGGCACGGCAATGC
CTTCATCGCATTCGCGGTCGGCGCGCTGTTCTCCTCGATCCTGCCGACCTTCACCTCTATCCTTCCGGATTGGTGGGGCA
CTTATGGCTGGTTCTTCGGCGTCGCCATCGGCGGAGCGATCTATTTCGTGCTGAGAATGGGCGCGCGGCGCAATCCGGCA
TTCGCCGCATGA

Upstream 100 bases:

>100_bases
AGCATTGTGTACAATGTGACTATTAAATTGTCAGATGCCTAAATGAAGCGCAGACTTCCTCCCGTGGTCGAGTCGCCACA
GGGGATATGGGAGGGCATTC

Downstream 100 bases:

>100_bases
CCAAATAGAAGGCGCCCGGCAACGGCCGGGCGCCTTTTACTTCAGAATTCCTGCAGACCTACGTTCAGCGGCGCGCGGCA
ACCGCAGGGATCGCTGCGCG

Product: putative nucleotide permease

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MSIRDPSPSLYNEDLAPAAERKWGAFSIFNVWTSDVHSLWGYYLAASLFLLCGSFVNFVIAIGIGSLVIFILMSLVGNAG
VRTGVPFPVLARASFGTFGANVPALVRAVVACFWYGAQTAAASGAIVALLIRNESLLAFHQNSHMLGHSTLELICYVIVW
ALQLLIIQRGMETVRKFQDWAGPAVWIMMLILAVYLVIKSGTFSFGAEIPRDVLIEKTKDAGVPGEPGSIAALAAVAATW
ITYFAALYLNFCDFSRYATSEKALRKGNLWGLPINLLAFCLVAGVTTTAAFTVYGEVLLHPEMISAKFDSWFLALLAALT
FAVATLGINVVANFVSPAFDFANVFPRQINFKRGGYVAALIALVLYPFAPWETGAAHFVNFIGSTMGPIFGIMMVDYYLI
RKSQLNVEALYRENGEFRFQNGWHGNAFIAFAVGALFSSILPTFTSILPDWWGTYGWFFGVAIGGAIYFVLRMGARRNPA
FAA

Sequences:

>Translated_483_residues
MSIRDPSPSLYNEDLAPAAERKWGAFSIFNVWTSDVHSLWGYYLAASLFLLCGSFVNFVIAIGIGSLVIFILMSLVGNAG
VRTGVPFPVLARASFGTFGANVPALVRAVVACFWYGAQTAAASGAIVALLIRNESLLAFHQNSHMLGHSTLELICYVIVW
ALQLLIIQRGMETVRKFQDWAGPAVWIMMLILAVYLVIKSGTFSFGAEIPRDVLIEKTKDAGVPGEPGSIAALAAVAATW
ITYFAALYLNFCDFSRYATSEKALRKGNLWGLPINLLAFCLVAGVTTTAAFTVYGEVLLHPEMISAKFDSWFLALLAALT
FAVATLGINVVANFVSPAFDFANVFPRQINFKRGGYVAALIALVLYPFAPWETGAAHFVNFIGSTMGPIFGIMMVDYYLI
RKSQLNVEALYRENGEFRFQNGWHGNAFIAFAVGALFSSILPTFTSILPDWWGTYGWFFGVAIGGAIYFVLRMGARRNPA
FAA
>Mature_482_residues
SIRDPSPSLYNEDLAPAAERKWGAFSIFNVWTSDVHSLWGYYLAASLFLLCGSFVNFVIAIGIGSLVIFILMSLVGNAGV
RTGVPFPVLARASFGTFGANVPALVRAVVACFWYGAQTAAASGAIVALLIRNESLLAFHQNSHMLGHSTLELICYVIVWA
LQLLIIQRGMETVRKFQDWAGPAVWIMMLILAVYLVIKSGTFSFGAEIPRDVLIEKTKDAGVPGEPGSIAALAAVAATWI
TYFAALYLNFCDFSRYATSEKALRKGNLWGLPINLLAFCLVAGVTTTAAFTVYGEVLLHPEMISAKFDSWFLALLAALTF
AVATLGINVVANFVSPAFDFANVFPRQINFKRGGYVAALIALVLYPFAPWETGAAHFVNFIGSTMGPIFGIMMVDYYLIR
KSQLNVEALYRENGEFRFQNGWHGNAFIAFAVGALFSSILPTFTSILPDWWGTYGWFFGVAIGGAIYFVLRMGARRNPAF
AA

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=476, Percent_Identity=27.1008403361345, Blast_Score=155, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6322219, Length=450, Percent_Identity=24.4444444444444, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6319495, Length=440, Percent_Identity=24.5454545454545, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319429, Length=470, Percent_Identity=23.1914893617021, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323266, Length=447, Percent_Identity=24.1610738255034, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6324766, Length=443, Percent_Identity=23.0248306997743, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324645, Length=443, Percent_Identity=21.4446952595937, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 52701; Mature: 52570

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIRDPSPSLYNEDLAPAAERKWGAFSIFNVWTSDVHSLWGYYLAASLFLLCGSFVNFVI
CCCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIGIGSLVIFILMSLVGNAGVRTGVPFPVLARASFGTFGANVPALVRAVVACFWYGAQTA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCHHHH
AASGAIVALLIRNESLLAFHQNSHMLGHSTLELICYVIVWALQLLIIQRGMETVRKFQDW
HCCCCEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGPAVWIMMLILAVYLVIKSGTFSFGAEIPRDVLIEKTKDAGVPGEPGSIAALAAVAATW
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
ITYFAALYLNFCDFSRYATSEKALRKGNLWGLPINLLAFCLVAGVTTTAAFTVYGEVLLH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
PEMISAKFDSWFLALLAALTFAVATLGINVVANFVSPAFDFANVFPRQINFKRGGYVAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCHHHHH
IALVLYPFAPWETGAAHFVNFIGSTMGPIFGIMMVDYYLIRKSQLNVEALYRENGEFRFQ
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEC
NGWHGNAFIAFAVGALFSSILPTFTSILPDWWGTYGWFFGVAIGGAIYFVLRMGARRNPA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
FAA
CCC
>Mature Secondary Structure 
SIRDPSPSLYNEDLAPAAERKWGAFSIFNVWTSDVHSLWGYYLAASLFLLCGSFVNFVI
CCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIGIGSLVIFILMSLVGNAGVRTGVPFPVLARASFGTFGANVPALVRAVVACFWYGAQTA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCHHHH
AASGAIVALLIRNESLLAFHQNSHMLGHSTLELICYVIVWALQLLIIQRGMETVRKFQDW
HCCCCEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGPAVWIMMLILAVYLVIKSGTFSFGAEIPRDVLIEKTKDAGVPGEPGSIAALAAVAATW
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
ITYFAALYLNFCDFSRYATSEKALRKGNLWGLPINLLAFCLVAGVTTTAAFTVYGEVLLH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
PEMISAKFDSWFLALLAALTFAVATLGINVVANFVSPAFDFANVFPRQINFKRGGYVAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCHHHHH
IALVLYPFAPWETGAAHFVNFIGSTMGPIFGIMMVDYYLIRKSQLNVEALYRENGEFRFQ
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEC
NGWHGNAFIAFAVGALFSSILPTFTSILPDWWGTYGWFFGVAIGGAIYFVLRMGARRNPA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
FAA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]