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The map label for this gene is treZ [H]

Identifier: 190894791

GI number: 190894791

Start: 482826

End: 484598

Strand: Reverse

Name: treZ [H]

Synonym: RHECIAT_PC0000456

Alternate gene names: 190894791

Gene position: 484598-482826 (Counterclockwise)

Preceding gene: 190894792

Following gene: 190894790

Centisome position: 44.4

GC content: 62.38

Gene sequence:

>1773_bases
ATGCCCTTGACCAACATGACGTTCGGCCCCGCCTTCAGCGAAGACGGCATTCTGTTCCGCCTCTGGGCTCCTCTGCATGA
AAGCGTGTCATTGAGGATCGAAGGCGCGGACCCTCGTCCGATGCAGGCGGGCGAAGATGGCTGGCATCGCTGCCTGGTCG
CGGATGCCGGAGCCGGCATGCGCTACCGTTTCGTGCTGCCCGACGGCCTCGAGGTTCCCGATCCGGCCTCGCGCTTCCAG
CCGCAGGATGTGCATGGCCCGAGCGAAGTGGTCGACCTTTCTTCCTATCGCTGGAACACCAGCGAATGGACCGGCCGGCC
TTGGGAAGAAATGATCATCTACGAACTGCATATCGGCTGCTTCACGCCAGAGGGTACTTTCAAGGCTGCGATTGAGCGGC
TCGACCATCTGCAGGCACTGGGCGTGACGGCGCTGCAGATCATGCCGCTCGCTGAATTCCCTGGTCGCTACAGCTGGGGC
TATGACGGCGTGCTACCCTACGCTCCTGACAGCAGCTACGGCCGGCCGGAAGATCTCATGGCGCTGGTGGACGCTGCACA
TGAGCGCGGCATCTCGGTCTTCCTCGACGCCGTCTATAATCACTTCGGCCCGGACGGGAACTATATCCCATCCTACGCGC
CGCTTTTTACCGATCATCACAAGACCCCCTGGGGCCACGGCGTCAATTACGACGGCGACGGCTCCGGGATGATCCGCGAA
TTCATCATCGAGAACGCCATCTACTGGATTACCGCGTTCCGGCTCGACGGTTTTCGCTTCGATGCCGTCCATGCCATCAA
GGACGACAGCAAGGAACATCTTCTGCATGCGCTCGCCCGCCGTGTTCGGGCAGCCGCCGGCGACAGGCACGTCCATCTGA
TCGTCGAGAACGAGGAGAACGACAGCGAGCTGCTGCAGCGCGATGAAAGCGGCGAGCCGAGCCTGTTCACGGCCCAGTGG
AACGACGACTTGCACCATGTGCTGCATATCGCTGCGACCGGCGAGACCTTCGGCTATTATGCCGACTACGCCGGTGATGC
CGACAAGCTTGGCCGCGCGCTGGCGGAAGGCTTCGTGTTTCAGGGCGAGCATATGCCCTATCGCGGCGGGAGCCGGGGCA
GGCCGAGCGGCCACCTGCCGCCGACTGCCTTCGTGTCCTTCATCCAGAACCACGACCAAATCGGCAACCGGGCGCTCGGC
GACCGCATCATGGCCGCAAGCCCGGCCGATGCCGTCAAGGCAGTCACATCAATCTACCTGCTGGCGCCCGAGATCCCGAT
GCTGTTCATGGGCGAGGAATGGGGCGCCGACGAGCCTTTTCCGTTTTTTTGCGATTTCGACGAGGATCTGAACGAAAAAG
TCCGGAAAGGCCGGCGCGAGGAGCTGTCGCGCCTGCCGGGCTTCGATGCCGGCGAGCTTCTCGACCCGACGGCGCCGTCG
ACCTTCGCTGCTGCAAAGCTCGATTGGGAGCAAATCGCCTCCTCGGAGACCCTCGACTTCTACAGGATGCTTCTCGGCCT
ACGACGTCAACGAATTATTCCCTTGCTGAAAGGAGCCGCCGGACATGCGACTTTCCAGTCGGCTGGAGCAGCGGTTGCGG
TGGCTTGGACGCTTGCCGGCAACCGGCGCCTTCATCTCACCGCCAATCTCAGTGACGAGGCCGCGAGGCTTGGCCATCGC
CATGAGGACGGCGAAACGATCTTCCGTCTCGGTCGCAATGACGATGGAGATCTTGCCCCATGGACGGTGATCTGGAGCAT
CGGCGAAGCGTAA

Upstream 100 bases:

>100_bases
GGCCTGCACGCTGCTGACACCACAGCTTCACACGTCCGCTTGCAGACGAAACTTTTTGCTCCTGCAAAAGTTACCCAGGT
TCCGTCCATTCATCAAAGGC

Downstream 100 bases:

>100_bases
GGGGGCCGAAATGAAGGCGATCAAATTATGAGGTCCTGGGAGCTCGAAAGTCTCGCCCGCCGGCACGGCATCATCCCGAC
AAGGCCCAGTCCCGACAATC

Product: putative 1,4-alpha-glucan branching protein

Products: NA

Alternate protein names: MTHase; 4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase; Glycosyltrehalose trehalohydrolase; GTHase; Maltooligosyl trehalose trehalohydrolase [H]

Number of amino acids: Translated: 590; Mature: 589

Protein sequence:

>590_residues
MPLTNMTFGPAFSEDGILFRLWAPLHESVSLRIEGADPRPMQAGEDGWHRCLVADAGAGMRYRFVLPDGLEVPDPASRFQ
PQDVHGPSEVVDLSSYRWNTSEWTGRPWEEMIIYELHIGCFTPEGTFKAAIERLDHLQALGVTALQIMPLAEFPGRYSWG
YDGVLPYAPDSSYGRPEDLMALVDAAHERGISVFLDAVYNHFGPDGNYIPSYAPLFTDHHKTPWGHGVNYDGDGSGMIRE
FIIENAIYWITAFRLDGFRFDAVHAIKDDSKEHLLHALARRVRAAAGDRHVHLIVENEENDSELLQRDESGEPSLFTAQW
NDDLHHVLHIAATGETFGYYADYAGDADKLGRALAEGFVFQGEHMPYRGGSRGRPSGHLPPTAFVSFIQNHDQIGNRALG
DRIMAASPADAVKAVTSIYLLAPEIPMLFMGEEWGADEPFPFFCDFDEDLNEKVRKGRREELSRLPGFDAGELLDPTAPS
TFAAAKLDWEQIASSETLDFYRMLLGLRRQRIIPLLKGAAGHATFQSAGAAVAVAWTLAGNRRLHLTANLSDEAARLGHR
HEDGETIFRLGRNDDGDLAPWTVIWSIGEA

Sequences:

>Translated_590_residues
MPLTNMTFGPAFSEDGILFRLWAPLHESVSLRIEGADPRPMQAGEDGWHRCLVADAGAGMRYRFVLPDGLEVPDPASRFQ
PQDVHGPSEVVDLSSYRWNTSEWTGRPWEEMIIYELHIGCFTPEGTFKAAIERLDHLQALGVTALQIMPLAEFPGRYSWG
YDGVLPYAPDSSYGRPEDLMALVDAAHERGISVFLDAVYNHFGPDGNYIPSYAPLFTDHHKTPWGHGVNYDGDGSGMIRE
FIIENAIYWITAFRLDGFRFDAVHAIKDDSKEHLLHALARRVRAAAGDRHVHLIVENEENDSELLQRDESGEPSLFTAQW
NDDLHHVLHIAATGETFGYYADYAGDADKLGRALAEGFVFQGEHMPYRGGSRGRPSGHLPPTAFVSFIQNHDQIGNRALG
DRIMAASPADAVKAVTSIYLLAPEIPMLFMGEEWGADEPFPFFCDFDEDLNEKVRKGRREELSRLPGFDAGELLDPTAPS
TFAAAKLDWEQIASSETLDFYRMLLGLRRQRIIPLLKGAAGHATFQSAGAAVAVAWTLAGNRRLHLTANLSDEAARLGHR
HEDGETIFRLGRNDDGDLAPWTVIWSIGEA
>Mature_589_residues
PLTNMTFGPAFSEDGILFRLWAPLHESVSLRIEGADPRPMQAGEDGWHRCLVADAGAGMRYRFVLPDGLEVPDPASRFQP
QDVHGPSEVVDLSSYRWNTSEWTGRPWEEMIIYELHIGCFTPEGTFKAAIERLDHLQALGVTALQIMPLAEFPGRYSWGY
DGVLPYAPDSSYGRPEDLMALVDAAHERGISVFLDAVYNHFGPDGNYIPSYAPLFTDHHKTPWGHGVNYDGDGSGMIREF
IIENAIYWITAFRLDGFRFDAVHAIKDDSKEHLLHALARRVRAAAGDRHVHLIVENEENDSELLQRDESGEPSLFTAQWN
DDLHHVLHIAATGETFGYYADYAGDADKLGRALAEGFVFQGEHMPYRGGSRGRPSGHLPPTAFVSFIQNHDQIGNRALGD
RIMAASPADAVKAVTSIYLLAPEIPMLFMGEEWGADEPFPFFCDFDEDLNEKVRKGRREELSRLPGFDAGELLDPTAPST
FAAAKLDWEQIASSETLDFYRMLLGLRRQRIIPLLKGAAGHATFQSAGAAVAVAWTLAGNRRLHLTANLSDEAARLGHRH
EDGETIFRLGRNDDGDLAPWTVIWSIGEA

Specific function: Catalyzes The Formation Of The Alpha-1,6-Glucosidic Linkages In Glycogen By Scission Of A 1,4-Alpha-Linked Oligosaccharide From Growing Alpha-1,4-Glucan Chains And The Subsequent Attachment Of The Oligosaccharide To The Alpha-1,6 Position. Has A Preferenc

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI189458812, Length=166, Percent_Identity=31.3253012048193, Blast_Score=76, Evalue=1e-13,
Organism=Escherichia coli, GI1789839, Length=279, Percent_Identity=27.5985663082437, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI2367229, Length=410, Percent_Identity=24.6341463414634, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17554896, Length=210, Percent_Identity=30, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI32564391, Length=210, Percent_Identity=30, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320826, Length=176, Percent_Identity=31.25, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- InterPro:   IPR015156
- InterPro:   IPR012768 [H]

Pfam domain/function: PF09071 Alpha-amyl_C; PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: =3.2.1.141 [H]

Molecular weight: Translated: 65623; Mature: 65492

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTNMTFGPAFSEDGILFRLWAPLHESVSLRIEGADPRPMQAGEDGWHRCLVADAGAGM
CCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCCE
RYRFVLPDGLEVPDPASRFQPQDVHGPSEVVDLSSYRWNTSEWTGRPWEEMIIYELHIGC
EEEEECCCCCCCCCCHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHEEEEEEEEEE
FTPEGTFKAAIERLDHLQALGVTALQIMPLAEFPGRYSWGYDGVLPYAPDSSYGRPEDLM
ECCCCHHHHHHHHHHHHHHHCHHHHEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHH
ALVDAAHERGISVFLDAVYNHFGPDGNYIPSYAPLFTDHHKTPWGHGVNYDGDGSGMIRE
HHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCHHHHH
FIIENAIYWITAFRLDGFRFDAVHAIKDDSKEHLLHALARRVRAAAGDRHVHLIVENEEN
HHHHHHEEEEEEEEECCEEEEEEHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
DSELLQRDESGEPSLFTAQWNDDLHHVLHIAATGETFGYYADYAGDADKLGRALAEGFVF
HHHHHHCCCCCCCCEEEEECCCCCEEEEEEEECCCCCCEEECCCCCHHHHHHHHHHCCEE
QGEHMPYRGGSRGRPSGHLPPTAFVSFIQNHDQIGNRALGDRIMAASPADAVKAVTSIYL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCHHHHHHHHHHHE
LAPEIPMLFMGEEWGADEPFPFFCDFDEDLNEKVRKGRREELSRLPGFDAGELLDPTAPS
ECCCCCEEEECCCCCCCCCCCEEECCCHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCC
TFAAAKLDWEQIASSETLDFYRMLLGLRRQRIIPLLKGAAGHATFQSAGAAVAVAWTLAG
CHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEEEEECC
NRRLHLTANLSDEAARLGHRHEDGETIFRLGRNDDGDLAPWTVIWSIGEA
CEEEEEEECCCHHHHHHCCCCCCCCCEEEECCCCCCCCCCEEEEEECCCC
>Mature Secondary Structure 
PLTNMTFGPAFSEDGILFRLWAPLHESVSLRIEGADPRPMQAGEDGWHRCLVADAGAGM
CCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCCE
RYRFVLPDGLEVPDPASRFQPQDVHGPSEVVDLSSYRWNTSEWTGRPWEEMIIYELHIGC
EEEEECCCCCCCCCCHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHEEEEEEEEEE
FTPEGTFKAAIERLDHLQALGVTALQIMPLAEFPGRYSWGYDGVLPYAPDSSYGRPEDLM
ECCCCHHHHHHHHHHHHHHHCHHHHEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHH
ALVDAAHERGISVFLDAVYNHFGPDGNYIPSYAPLFTDHHKTPWGHGVNYDGDGSGMIRE
HHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCHHHHH
FIIENAIYWITAFRLDGFRFDAVHAIKDDSKEHLLHALARRVRAAAGDRHVHLIVENEEN
HHHHHHEEEEEEEEECCEEEEEEHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
DSELLQRDESGEPSLFTAQWNDDLHHVLHIAATGETFGYYADYAGDADKLGRALAEGFVF
HHHHHHCCCCCCCCEEEEECCCCCEEEEEEEECCCCCCEEECCCCCHHHHHHHHHHCCEE
QGEHMPYRGGSRGRPSGHLPPTAFVSFIQNHDQIGNRALGDRIMAASPADAVKAVTSIYL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCHHHHHHHHHHHE
LAPEIPMLFMGEEWGADEPFPFFCDFDEDLNEKVRKGRREELSRLPGFDAGELLDPTAPS
ECCCCCEEEECCCCCCCCCCCEEECCCHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCC
TFAAAKLDWEQIASSETLDFYRMLLGLRRQRIIPLLKGAAGHATFQSAGAAVAVAWTLAG
CHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEEEEECC
NRRLHLTANLSDEAARLGHRHEDGETIFRLGRNDDGDLAPWTVIWSIGEA
CEEEEEEECCCHHHHHHCCCCCCCCCEEEECCCCCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8987868; 10926520 [H]