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The map label for this gene is ntaB [H]

Identifier: 190894726

GI number: 190894726

Start: 413809

End: 414801

Strand: Reverse

Name: ntaB [H]

Synonym: RHECIAT_PC0000391

Alternate gene names: 190894726

Gene position: 414801-413809 (Counterclockwise)

Preceding gene: 190894727

Following gene: 190894725

Centisome position: 38.0

GC content: 64.25

Gene sequence:

>993_bases
ATGGCGGAGACAGCGGCAAGGACGGATGTGCCGATCGACCCGATCGCACTGCGCCGGGCCTTCGGAACCTTCGTGACGGG
TGTGACCGTCATCACCACGCGCGACGAGGACGGGACCCCGCGCGGCATGACCGCGAATTCGTTTACATCAGTGTCGCTCG
ATCCGCCGCTGCTTCTCGTCTGCGTCGCGAAGGCGGCCTCCAGTTACCGGGCCTTCACCAATGCCGGCTGTTTTGCGGTC
AATATCCTTCATGAAGGTCAGGTCGATATCTCAGGCAAATTCGCCTCGAAGTCTCCCGACAAATTCCAGTCGGTCACCCA
CGATCACATCCATACCGGCGCGCCGGTTTTGACCGACAGTCTCACATGGTTCGACTGCACCACCTACAGCGCCGTCGATG
CTGGCGACCATACGGTTCTGATCGGCCAGGTCCGCGCCTTCGGCACTAGCCCGACCGCACCGCTCGGTTTCTGCCGCGGC
CGTTACGCCAGCGTCAAGGATCCGCTGCCCGCGGGCTGGCTCGCCTCGCATGGGATGATCATCGGCTACTTGATCGAGGC
CGGCGACGGCCTGCTGCTGCGCTCGGACGGGAGGGGCGGCTGGGTGTTGCCATCGGCACACCGCCGCAAGGCCGACAGCC
AACTGGTGGTGGAGGGCGGCGATGCGCTGCGCCTCGTACCGGAAGAGACCTTCCTCTATTCCGTCTTCGACGTCGCCGAC
AGCGATCCCGGCTATCTCGTCTATCGCGCCCGATTGGCAGGCGAGGGGGAAGAGGTCAACCTGCCTGCCGATCTCCGCTT
CTTCGGCATCGACGATCTGCCCTACGAGGCGATCGCCACCCATGAACTGCGCTCCATGCTACGCCGCTATGTACGCGAGC
GGCAGGCGGGTCGCTTCGGCATCTACATGGATTCGGACGATGGCGGCCGCGTCGCGATGATCGACGGCGAAGCCCGTGCC
TGGCTTCAAGCCTCCCAAGACCAAGGACAGTGA

Upstream 100 bases:

>100_bases
ATCGAGGCAGAAGTTTTTCACCCCATCCTACGAAGCCTGGGAAGAACGGTCGAAGCGGCAGCGATAGCCTCCTTTCAACA
AGCAGAGGAGGCTCAAAGGC

Downstream 100 bases:

>100_bases
CATGAAGACGACAGTCAGCTCCATCGAAGGTTCGCGACAGGGCCTGTTCATCAATGGCGAATTCGTGGCGCCGAAGGCCG
GCAACTACATTGCCAGCCAC

Product: putative nitrilotriacetate monooxygenase component B protein

Products: NAD(+); reduced flavin [C]

Alternate protein names: NTA monooxygenase component B; NTA-MO B [H]

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MAETAARTDVPIDPIALRRAFGTFVTGVTVITTRDEDGTPRGMTANSFTSVSLDPPLLLVCVAKAASSYRAFTNAGCFAV
NILHEGQVDISGKFASKSPDKFQSVTHDHIHTGAPVLTDSLTWFDCTTYSAVDAGDHTVLIGQVRAFGTSPTAPLGFCRG
RYASVKDPLPAGWLASHGMIIGYLIEAGDGLLLRSDGRGGWVLPSAHRRKADSQLVVEGGDALRLVPEETFLYSVFDVAD
SDPGYLVYRARLAGEGEEVNLPADLRFFGIDDLPYEAIATHELRSMLRRYVRERQAGRFGIYMDSDDGGRVAMIDGEARA
WLQASQDQGQ

Sequences:

>Translated_330_residues
MAETAARTDVPIDPIALRRAFGTFVTGVTVITTRDEDGTPRGMTANSFTSVSLDPPLLLVCVAKAASSYRAFTNAGCFAV
NILHEGQVDISGKFASKSPDKFQSVTHDHIHTGAPVLTDSLTWFDCTTYSAVDAGDHTVLIGQVRAFGTSPTAPLGFCRG
RYASVKDPLPAGWLASHGMIIGYLIEAGDGLLLRSDGRGGWVLPSAHRRKADSQLVVEGGDALRLVPEETFLYSVFDVAD
SDPGYLVYRARLAGEGEEVNLPADLRFFGIDDLPYEAIATHELRSMLRRYVRERQAGRFGIYMDSDDGGRVAMIDGEARA
WLQASQDQGQ
>Mature_329_residues
AETAARTDVPIDPIALRRAFGTFVTGVTVITTRDEDGTPRGMTANSFTSVSLDPPLLLVCVAKAASSYRAFTNAGCFAVN
ILHEGQVDISGKFASKSPDKFQSVTHDHIHTGAPVLTDSLTWFDCTTYSAVDAGDHTVLIGQVRAFGTSPTAPLGFCRGR
YASVKDPLPAGWLASHGMIIGYLIEAGDGLLLRSDGRGGWVLPSAHRRKADSQLVVEGGDALRLVPEETFLYSVFDVADS
DPGYLVYRARLAGEGEEVNLPADLRFFGIDDLPYEAIATHELRSMLRRYVRERQAGRFGIYMDSDDGGRVAMIDGEARAW
LQASQDQGQ

Specific function: Hydroxylation of nitrilotriacetate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081819, Length=159, Percent_Identity=32.0754716981132, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.6.8.- [C]

Molecular weight: Translated: 35692; Mature: 35561

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETAARTDVPIDPIALRRAFGTFVTGVTVITTRDEDGTPRGMTANSFTSVSLDPPLLLV
CCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCEEEECCCCCEEEH
CVAKAASSYRAFTNAGCFAVNILHEGQVDISGKFASKSPDKFQSVTHDHIHTGAPVLTDS
HHHHHHHHHHHHCCCCEEEEEEEECCCEEECCEECCCCCHHHHHHHHHHHCCCCCEEECC
LTWFDCTTYSAVDAGDHTVLIGQVRAFGTSPTAPLGFCRGRYASVKDPLPAGWLASHGMI
CEEEEECCCCEECCCCCEEEEEEEEECCCCCCCCHHHHCCCCCCCCCCCCCCHHHHCCEE
IGYLIEAGDGLLLRSDGRGGWVLPSAHRRKADSQLVVEGGDALRLVPEETFLYSVFDVAD
EEEEEECCCEEEEEECCCCCEECCCHHHCCCCCEEEEECCCEEEEECCHHEEEEEEECCC
SDPGYLVYRARLAGEGEEVNLPADLRFFGIDDLPYEAIATHELRSMLRRYVRERQAGRFG
CCCCEEEEEEEECCCCCEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEE
IYMDSDDGGRVAMIDGEARAWLQASQDQGQ
EEEECCCCCEEEEEECCCCEEEECCCCCCC
>Mature Secondary Structure 
AETAARTDVPIDPIALRRAFGTFVTGVTVITTRDEDGTPRGMTANSFTSVSLDPPLLLV
CCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCEEEECCCCCEEEH
CVAKAASSYRAFTNAGCFAVNILHEGQVDISGKFASKSPDKFQSVTHDHIHTGAPVLTDS
HHHHHHHHHHHHCCCCEEEEEEEECCCEEECCEECCCCCHHHHHHHHHHHCCCCCEEECC
LTWFDCTTYSAVDAGDHTVLIGQVRAFGTSPTAPLGFCRGRYASVKDPLPAGWLASHGMI
CEEEEECCCCEECCCCCEEEEEEEEECCCCCCCCHHHHCCCCCCCCCCCCCCHHHHCCEE
IGYLIEAGDGLLLRSDGRGGWVLPSAHRRKADSQLVVEGGDALRLVPEETFLYSVFDVAD
EEEEEECCCEEEEEECCCCCEECCCHHHCCCCCEEEEECCCEEEEECCHHEEEEEEECCC
SDPGYLVYRARLAGEGEEVNLPADLRFFGIDDLPYEAIATHELRSMLRRYVRERQAGRFG
CCCCEEEEEEEECCCCCEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEE
IYMDSDDGGRVAMIDGEARAWLQASQDQGQ
EEEECCCCCEEEEEECCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; flavin [C]

Specific reaction: NADH + flavin = NAD(+) + reduced flavin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8892809; 9023192; 1735711 [H]