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The map label for this gene is glpKc [H]

Identifier: 190894662

GI number: 190894662

Start: 349988

End: 351490

Strand: Reverse

Name: glpKc [H]

Synonym: RHECIAT_PC0000325

Alternate gene names: 190894662

Gene position: 351490-349988 (Counterclockwise)

Preceding gene: 190894663

Following gene: 190894657

Centisome position: 32.2

GC content: 64.4

Gene sequence:

>1503_bases
ATGAGCGGCTATATTCTTTCGATCGACCAGGGCACCACGTCTTCGCGCGCGATTATCTTCGATGGCGGCATGAAAATGGC
GGGGTCCGCGCAGGCGGAGATCACCCAATATTATCCGCAGCCCGGATGGGTGGAGCACGACGCGGCCGAAATCTGGCAGT
CCGTCGCCGATACGGCGCGCAAGGCGATCGCCGATGCCGGTTTGAAGCCTACCGATATTTCCGCCGTCGGCATCACCAAC
CAGCGCGAGACGGCGGTCGTCTGGGATCGCCGGTCCGGAACTCCTCTTCACCGCGCGATCGTCTGGCAGGACCGGCGCAC
AGCGGAAATGTGCGAAAGCCTGAAGGCGGACGGATATGAGAAGCTGTTCAGCGCCAAGACCGGGCTGCTGCTCGATCCGT
ATTTTTCGGGGACCAAGCTGCGCTGGCTGCTCGACAATGTCGATGGGCTGCGCGAGAAGGCGGAGGCGGGCGAGGTCTGC
TTCGGTACGATCGACAGCTGGTTGATCTACAAGCTGACCGGCGGCCGCGTGCACGCGACCGATGCTACCAATGCATCGCG
AACGCTGCTCTACAATATCGATGACGGCGCCTGGGACGCGGAACTGCTCGGCATTCTCGGTATTCCCCCTGCCATGCTGC
CCGAGGTCAAGGAATGCGCCGACGATTTCGGCCGCGTCGACAAAGCACTGCTCGGCGCCGAGCTTCCGATCCTCGGGGTC
GCCGGCGACCAGCAGGCAGCCGCGATGGGCAATGCCTGCTTCGAGCCCGGCATGATGAAATCCACCTATGGCACCGGCTG
TTTCGCCTTGCTGAATACCGGCCGGGACCGTGTTTCCTCGTCCAACCGGATGCTGACGACCATCGCCTGCCGGCTCGACG
GCGAGACCACTTATGCCCTCGAAGGCTCGATCTTCATCGCCGGCGCCGCCGTGCAGTGGCTGCGCGATGGGCTCGGCATC
ATCGAGCAGGCGTCCGAGGCAGGCGTGCTCGCGGCCAAGGCTGATCCCGGGCAGCAGGTCTATATCGTCCCCGCCTTCAC
CGGGCTGGGCGCGCCTTATTGGGATCCGGCGGCGCGCGGCGCGATCTTCGGCCTGACGCGCAACAGTGGACCGGCGGAAT
TTGCCCGCGCCGTGCTTGAATCCGTCGCCTATCAGACGCTGGACCTGCTTGTGGCGATGAAGAAGGACTGGGGTGTCAAC
CAGCTGGAAACCGTGCTGCGAGTCGACGGTGGCATGGCGGCTTCGGACTGGACGATGCAATGCCTCGCCGACATGACCGG
AAATCCAGTCGACCGCTCGGCAATCCGCGAGACGACGGCGCTTGGTGCGGCCTGGCTTGCCGGTTCGAAGGCCGGCATCT
GGCCCGGCAGGCGGGAGTTTGCGCAAGCCTGGGTCTGCGACCGCCGCTTCAGCCCTTCGATGGAGGAGGGCGAGCGTCAG
GCCAAGATCATCGGCTGGAAGAATGCCGTATCGCGGATGATCTCGGACGCTTCGGTGGGATAG

Upstream 100 bases:

>100_bases
GCCACCGGCAGCAGGCCGGTGGCACCCACAATCTTTCTCTCATGACATCCCCGCATCCGCCCTCCTGGGCGGAGCGGTTT
CGTGCGAGCCGGAGACGGAT

Downstream 100 bases:

>100_bases
CCGCTCGTCTCGTGGGCAAACTATGGCTGTGCTTCGCCGGCCGGATAGCCTTCACACCTCCATCGTATGATGTGGGCGCA
ATGCAATTGTACAATGATCA

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MSGYILSIDQGTTSSRAIIFDGGMKMAGSAQAEITQYYPQPGWVEHDAAEIWQSVADTARKAIADAGLKPTDISAVGITN
QRETAVVWDRRSGTPLHRAIVWQDRRTAEMCESLKADGYEKLFSAKTGLLLDPYFSGTKLRWLLDNVDGLREKAEAGEVC
FGTIDSWLIYKLTGGRVHATDATNASRTLLYNIDDGAWDAELLGILGIPPAMLPEVKECADDFGRVDKALLGAELPILGV
AGDQQAAAMGNACFEPGMMKSTYGTGCFALLNTGRDRVSSSNRMLTTIACRLDGETTYALEGSIFIAGAAVQWLRDGLGI
IEQASEAGVLAAKADPGQQVYIVPAFTGLGAPYWDPAARGAIFGLTRNSGPAEFARAVLESVAYQTLDLLVAMKKDWGVN
QLETVLRVDGGMAASDWTMQCLADMTGNPVDRSAIRETTALGAAWLAGSKAGIWPGRREFAQAWVCDRRFSPSMEEGERQ
AKIIGWKNAVSRMISDASVG

Sequences:

>Translated_500_residues
MSGYILSIDQGTTSSRAIIFDGGMKMAGSAQAEITQYYPQPGWVEHDAAEIWQSVADTARKAIADAGLKPTDISAVGITN
QRETAVVWDRRSGTPLHRAIVWQDRRTAEMCESLKADGYEKLFSAKTGLLLDPYFSGTKLRWLLDNVDGLREKAEAGEVC
FGTIDSWLIYKLTGGRVHATDATNASRTLLYNIDDGAWDAELLGILGIPPAMLPEVKECADDFGRVDKALLGAELPILGV
AGDQQAAAMGNACFEPGMMKSTYGTGCFALLNTGRDRVSSSNRMLTTIACRLDGETTYALEGSIFIAGAAVQWLRDGLGI
IEQASEAGVLAAKADPGQQVYIVPAFTGLGAPYWDPAARGAIFGLTRNSGPAEFARAVLESVAYQTLDLLVAMKKDWGVN
QLETVLRVDGGMAASDWTMQCLADMTGNPVDRSAIRETTALGAAWLAGSKAGIWPGRREFAQAWVCDRRFSPSMEEGERQ
AKIIGWKNAVSRMISDASVG
>Mature_499_residues
SGYILSIDQGTTSSRAIIFDGGMKMAGSAQAEITQYYPQPGWVEHDAAEIWQSVADTARKAIADAGLKPTDISAVGITNQ
RETAVVWDRRSGTPLHRAIVWQDRRTAEMCESLKADGYEKLFSAKTGLLLDPYFSGTKLRWLLDNVDGLREKAEAGEVCF
GTIDSWLIYKLTGGRVHATDATNASRTLLYNIDDGAWDAELLGILGIPPAMLPEVKECADDFGRVDKALLGAELPILGVA
GDQQAAAMGNACFEPGMMKSTYGTGCFALLNTGRDRVSSSNRMLTTIACRLDGETTYALEGSIFIAGAAVQWLRDGLGII
EQASEAGVLAAKADPGQQVYIVPAFTGLGAPYWDPAARGAIFGLTRNSGPAEFARAVLESVAYQTLDLLVAMKKDWGVNQ
LETVLRVDGGMAASDWTMQCLADMTGNPVDRSAIRETTALGAAWLAGSKAGIWPGRREFAQAWVCDRRFSPSMEEGERQA
KIIGWKNAVSRMISDASVG

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI189409120, Length=499, Percent_Identity=43.8877755511022, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI4504007, Length=499, Percent_Identity=43.8877755511022, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI42794763, Length=504, Percent_Identity=43.452380952381, Blast_Score=381, Evalue=1e-106,
Organism=Homo sapiens, GI41393575, Length=499, Percent_Identity=42.2845691382766, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI88196792, Length=511, Percent_Identity=30.9197651663405, Blast_Score=290, Evalue=3e-78,
Organism=Escherichia coli, GI1790361, Length=493, Percent_Identity=51.7241379310345, Blast_Score=530, Evalue=1e-152,
Organism=Escherichia coli, GI1790005, Length=467, Percent_Identity=25.6959314775161, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1789987, Length=454, Percent_Identity=27.3127753303965, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1787789, Length=502, Percent_Identity=26.0956175298805, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789168, Length=493, Percent_Identity=24.7464503042596, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17535599, Length=495, Percent_Identity=39.3939393939394, Blast_Score=338, Evalue=5e-93,
Organism=Caenorhabditis elegans, GI71986140, Length=518, Percent_Identity=25.2895752895753, Blast_Score=203, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI71986147, Length=298, Percent_Identity=27.5167785234899, Blast_Score=127, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6321755, Length=481, Percent_Identity=36.5904365904366, Blast_Score=268, Evalue=2e-72,
Organism=Drosophila melanogaster, GI17864214, Length=507, Percent_Identity=41.6173570019724, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24655555, Length=508, Percent_Identity=38.7795275590551, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24655560, Length=508, Percent_Identity=38.7795275590551, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24655570, Length=508, Percent_Identity=38.7795275590551, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24655574, Length=508, Percent_Identity=38.7795275590551, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24655565, Length=508, Percent_Identity=38.7795275590551, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24654573, Length=448, Percent_Identity=42.8571428571429, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI19922058, Length=528, Percent_Identity=31.4393939393939, Blast_Score=262, Evalue=4e-70,
Organism=Drosophila melanogaster, GI24652871, Length=494, Percent_Identity=32.1862348178138, Blast_Score=252, Evalue=5e-67,
Organism=Drosophila melanogaster, GI24656367, Length=514, Percent_Identity=29.5719844357977, Blast_Score=213, Evalue=4e-55,
Organism=Drosophila melanogaster, GI21357867, Length=525, Percent_Identity=28.3809523809524, Blast_Score=208, Evalue=9e-54,
Organism=Drosophila melanogaster, GI24656372, Length=348, Percent_Identity=29.3103448275862, Blast_Score=141, Evalue=9e-34,
Organism=Drosophila melanogaster, GI45552909, Length=281, Percent_Identity=27.4021352313167, Blast_Score=102, Evalue=7e-22,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 53759; Mature: 53628

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGYILSIDQGTTSSRAIIFDGGMKMAGSAQAEITQYYPQPGWVEHDAAEIWQSVADTAR
CCCEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
KAIADAGLKPTDISAVGITNQRETAVVWDRRSGTPLHRAIVWQDRRTAEMCESLKADGYE
HHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCCHHHEEEECCCCHHHHHHHHCCCCHH
KLFSAKTGLLLDPYFSGTKLRWLLDNVDGLREKAEAGEVCFGTIDSWLIYKLTGGRVHAT
HHHHCCCCCEECCCCCCCEEEEEHHHHHHHHHHHHCCCEEEECCCCEEEEEEECCEEEEC
DATNASRTLLYNIDDGAWDAELLGILGIPPAMLPEVKECADDFGRVDKALLGAELPILGV
CCCCCCCEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
AGDQQAAAMGNACFEPGMMKSTYGTGCFALLNTGRDRVSSSNRMLTTIACRLDGETTYAL
CCCCHHHHHCCHHCCCCCCCCCCCCCHHHHHHCCHHHHCCCCCEEEEEEEEECCCEEEEE
EGSIFIAGAAVQWLRDGLGIIEQASEAGVLAAKADPGQQVYIVPAFTGLGAPYWDPAARG
CCCEEEEHHHHHHHHHCHHHHHHHCCCCEEEEECCCCCEEEEEEECCCCCCCCCCHHHCC
AIFGLTRNSGPAEFARAVLESVAYQTLDLLVAMKKDWGVNQLETVLRVDGGMAASDWTMQ
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHH
CLADMTGNPVDRSAIRETTALGAAWLAGSKAGIWPGRREFAQAWVCDRRFSPSMEEGERQ
HHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHCCCC
AKIIGWKNAVSRMISDASVG
EEEEEHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SGYILSIDQGTTSSRAIIFDGGMKMAGSAQAEITQYYPQPGWVEHDAAEIWQSVADTAR
CCEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
KAIADAGLKPTDISAVGITNQRETAVVWDRRSGTPLHRAIVWQDRRTAEMCESLKADGYE
HHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCCHHHEEEECCCCHHHHHHHHCCCCHH
KLFSAKTGLLLDPYFSGTKLRWLLDNVDGLREKAEAGEVCFGTIDSWLIYKLTGGRVHAT
HHHHCCCCCEECCCCCCCEEEEEHHHHHHHHHHHHCCCEEEECCCCEEEEEEECCEEEEC
DATNASRTLLYNIDDGAWDAELLGILGIPPAMLPEVKECADDFGRVDKALLGAELPILGV
CCCCCCCEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
AGDQQAAAMGNACFEPGMMKSTYGTGCFALLNTGRDRVSSSNRMLTTIACRLDGETTYAL
CCCCHHHHHCCHHCCCCCCCCCCCCCHHHHHHCCHHHHCCCCCEEEEEEEEECCCEEEEE
EGSIFIAGAAVQWLRDGLGIIEQASEAGVLAAKADPGQQVYIVPAFTGLGAPYWDPAARG
CCCEEEEHHHHHHHHHCHHHHHHHCCCCEEEEECCCCCEEEEEEECCCCCCCCCCHHHCC
AIFGLTRNSGPAEFARAVLESVAYQTLDLLVAMKKDWGVNQLETVLRVDGGMAASDWTMQ
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHH
CLADMTGNPVDRSAIRETTALGAAWLAGSKAGIWPGRREFAQAWVCDRRFSPSMEEGERQ
HHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHCCCC
AKIIGWKNAVSRMISDASVG
EEEEEHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]