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The map label for this gene is oppA [H]

Identifier: 190894657

GI number: 190894657

Start: 343260

End: 344897

Strand: Reverse

Name: oppA [H]

Synonym: RHECIAT_PC0000320

Alternate gene names: 190894657

Gene position: 344897-343260 (Counterclockwise)

Preceding gene: 190894662

Following gene: 190894656

Centisome position: 31.6

GC content: 61.84

Gene sequence:

>1638_bases
ATGTCTCTTGATCAATCTGACAAAACCAATTTATCGCGTCGCAATGCATTGAAACTCGGCCTCGCCGCTGGCGTCGGGCT
GACCGTGTTCGGGATGAATGCCCGCATCGTGCTGGCCGATGAAGGCCAGGTCCTGAAGGTCGCCCATCCGGCCTTCGACC
AGGACTGGTCGCCGCTGCGTGGCGGCGGCAGAACCTTCCGCTGGAACTCGATCTGGTGGGCTTCGCCGATGTATTTTGAC
AGTCAGGGCAATATCAAGCCCTACGTCTTCACCAACTGGGAATCGGCCGACAACACGGTCTGGACCTTCAAGATCGACCC
CAAGGCCGTCTTCTCCGACGGCAGCAAGATCACCTCGGCCGACGTCAAGGGATCGTGGGAAGTCGCCTCGATGCCGAACA
CCAAGAGCCAGCGCGCCGACCAGGTGCTGAGCAAGGTCAAGGGCTACGCCGAAATCGCCGCCGGCTCAGGCAACGAACTA
ACCGGGGTCGCGACGCCCGACGAGGGGACGGTGGTCGTGACGCTCGCCGCCGCCGATCCGATCTTCTTCATGCGTCTCGC
AAATCACATTGCACCGATCACCAAGGCGTCGCAGTCGCGCGGCAGCGACGGCGAGGAAATCATCGACTGGTACAAGCCGG
AGAACAAGCCGGTTTTCTCCGGCCCGTTCAAGCTGACGAGCATCGATATCGATGCCGGCAAGATCACCTTCGAGCCAAAT
GAGAAATTCTTCGGGCCGAAGCCCAAGCTTGCCCGCATCGACATCACTTCGATCGAGGACAATGTCACTGCGACGTCGCT
GATCAAGTCGGGCGAGTTCAACGCCCATACGGAGCTCGTCACCTCGACGATCATTCAGGATCTCGGCCCGGAATTCTCGG
CCGGCCCGCTGATCCCGACCAGCCAGCACTTCTGGTTCAACACTTCCCGCGCGCCGATGGACGATCCGAAGGTCCGCCAG
GCGCTGATCATGGCGGTCGATCGCGACGGCCTGTTCAAGGCGTCCTATCCGGATGGCCCCCACAAGAAGGCCGACCAGAT
CCTCAACTCGGTCCCCGGCGCCGACAATTCCGGTTTCGAGCCTTTTCCCTATGATCCGGCAGCCGCCAAGAAGCTGCTTG
CCGAATCGAGCTATGGCGGGCCCGAGCGCCTGCCGAAGCTCCTCTTCGTCGGCATCTCGGCGCCGGCCATTCAGGCCGCT
GCCCAGTTCATCGCCGAGCAATGGCGCCAGAACCTCGGTATCACGGCCGTCGACATGAAGCCGCAGCAGGACGCCTATGC
CGGTCCGGATCAGAACTCGGTCCAGATCTTCCGCGACGACGTCGGCACCCGTGTTCCCGACGCTGTTTCCTATCTGGCGG
GCAGCATCGCCTCGACCTCGTCGAACGCGCAGAACAAGCTCGGCGGATACAAGAACGACAAGGTCGACAGCGCCCTTGCC
GAAGCGGCGACCAAGGCTGCGGACGATCCGCAGCGCGTTACTCTCGCCCAGGAGGCCCAGAAGGCGTTCCGCGACGATTG
GGCCTTCATTCCATGGTATTCTCAGGCGATGTCGCGCTGGGCCACCAAGGAGGTCAAGGGCATGGAGAAGAACCTCGACT
GGCAGATCGCCGAACCCTGGAACATTTCGATCGGCTGA

Upstream 100 bases:

>100_bases
TGTTGACATATTTACAATAACGAGAGAACGATAGGCGATGGATTGTGCTGCGCCTGGCAGCGCAGGGAGAGCGCTGCCGA
TTTCACCTGGGAGGAATGCC

Downstream 100 bases:

>100_bases
TTGGACCTATCGTTTCTGACAATGAGCGCGCGAGGGCCGCAAGGCCTTGGCGCAAGTTCGAGAAGAAGAGATGCGATCTG
CCGGGCTTTAGCAAAGGGCG

Product: putative oligopeptide ABC transporter substrate-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MSLDQSDKTNLSRRNALKLGLAAGVGLTVFGMNARIVLADEGQVLKVAHPAFDQDWSPLRGGGRTFRWNSIWWASPMYFD
SQGNIKPYVFTNWESADNTVWTFKIDPKAVFSDGSKITSADVKGSWEVASMPNTKSQRADQVLSKVKGYAEIAAGSGNEL
TGVATPDEGTVVVTLAAADPIFFMRLANHIAPITKASQSRGSDGEEIIDWYKPENKPVFSGPFKLTSIDIDAGKITFEPN
EKFFGPKPKLARIDITSIEDNVTATSLIKSGEFNAHTELVTSTIIQDLGPEFSAGPLIPTSQHFWFNTSRAPMDDPKVRQ
ALIMAVDRDGLFKASYPDGPHKKADQILNSVPGADNSGFEPFPYDPAAAKKLLAESSYGGPERLPKLLFVGISAPAIQAA
AQFIAEQWRQNLGITAVDMKPQQDAYAGPDQNSVQIFRDDVGTRVPDAVSYLAGSIASTSSNAQNKLGGYKNDKVDSALA
EAATKAADDPQRVTLAQEAQKAFRDDWAFIPWYSQAMSRWATKEVKGMEKNLDWQIAEPWNISIG

Sequences:

>Translated_545_residues
MSLDQSDKTNLSRRNALKLGLAAGVGLTVFGMNARIVLADEGQVLKVAHPAFDQDWSPLRGGGRTFRWNSIWWASPMYFD
SQGNIKPYVFTNWESADNTVWTFKIDPKAVFSDGSKITSADVKGSWEVASMPNTKSQRADQVLSKVKGYAEIAAGSGNEL
TGVATPDEGTVVVTLAAADPIFFMRLANHIAPITKASQSRGSDGEEIIDWYKPENKPVFSGPFKLTSIDIDAGKITFEPN
EKFFGPKPKLARIDITSIEDNVTATSLIKSGEFNAHTELVTSTIIQDLGPEFSAGPLIPTSQHFWFNTSRAPMDDPKVRQ
ALIMAVDRDGLFKASYPDGPHKKADQILNSVPGADNSGFEPFPYDPAAAKKLLAESSYGGPERLPKLLFVGISAPAIQAA
AQFIAEQWRQNLGITAVDMKPQQDAYAGPDQNSVQIFRDDVGTRVPDAVSYLAGSIASTSSNAQNKLGGYKNDKVDSALA
EAATKAADDPQRVTLAQEAQKAFRDDWAFIPWYSQAMSRWATKEVKGMEKNLDWQIAEPWNISIG
>Mature_544_residues
SLDQSDKTNLSRRNALKLGLAAGVGLTVFGMNARIVLADEGQVLKVAHPAFDQDWSPLRGGGRTFRWNSIWWASPMYFDS
QGNIKPYVFTNWESADNTVWTFKIDPKAVFSDGSKITSADVKGSWEVASMPNTKSQRADQVLSKVKGYAEIAAGSGNELT
GVATPDEGTVVVTLAAADPIFFMRLANHIAPITKASQSRGSDGEEIIDWYKPENKPVFSGPFKLTSIDIDAGKITFEPNE
KFFGPKPKLARIDITSIEDNVTATSLIKSGEFNAHTELVTSTIIQDLGPEFSAGPLIPTSQHFWFNTSRAPMDDPKVRQA
LIMAVDRDGLFKASYPDGPHKKADQILNSVPGADNSGFEPFPYDPAAAKKLLAESSYGGPERLPKLLFVGISAPAIQAAA
QFIAEQWRQNLGITAVDMKPQQDAYAGPDQNSVQIFRDDVGTRVPDAVSYLAGSIASTSSNAQNKLGGYKNDKVDSALAE
AATKAADDPQRVTLAQEAQKAFRDDWAFIPWYSQAMSRWATKEVKGMEKNLDWQIAEPWNISIG

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, it binds peptides up to five amino acids long with high affinity [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=454, Percent_Identity=25.5506607929515, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI87081878, Length=359, Percent_Identity=28.133704735376, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1789397, Length=468, Percent_Identity=25.4273504273504, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1787052, Length=452, Percent_Identity=25.6637168141593, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1789887, Length=461, Percent_Identity=23.8611713665944, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59406; Mature: 59275

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLDQSDKTNLSRRNALKLGLAAGVGLTVFGMNARIVLADEGQVLKVAHPAFDQDWSPLR
CCCCCCCCCCCHHHHHHEEHHHCCCCEEEEECCEEEEEECCCCEEEEECCCCCCCCCCCC
GGGRTFRWNSIWWASPMYFDSQGNIKPYVFTNWESADNTVWTFKIDPKAVFSDGSKITSA
CCCCEEEECCEEECCCEEECCCCCCCCEEEECCCCCCCEEEEEEECCHHEECCCCEEEEC
DVKGSWEVASMPNTKSQRADQVLSKVKGYAEIAAGSGNELTGVATPDEGTVVVTLAAADP
CCCCCEEEECCCCCHHHHHHHHHHHHCCHHEEECCCCCCEEEEECCCCCEEEEEEECCCH
IFFMRLANHIAPITKASQSRGSDGEEIIDWYKPENKPVFSGPFKLTSIDIDAGKITFEPN
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEECCCEEEEEEEECCCEEEECCC
EKFFGPKPKLARIDITSIEDNVTATSLIKSGEFNAHTELVTSTIIQDLGPEFSAGPLIPT
CCCCCCCCCEEEEEEECCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
SQHFWFNTSRAPMDDPKVRQALIMAVDRDGLFKASYPDGPHKKADQILNSVPGADNSGFE
CCCEEEECCCCCCCCHHHHHHHHEEECCCCCEECCCCCCCHHHHHHHHHHCCCCCCCCCC
PFPYDPAAAKKLLAESSYGGPERLPKLLFVGISAPAIQAAAQFIAEQWRQNLGITAVDMK
CCCCCHHHHHHHHHHCCCCCHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHCCEEEEECC
PQQDAYAGPDQNSVQIFRDDVGTRVPDAVSYLAGSIASTSSNAQNKLGGYKNDKVDSALA
CCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHH
EAATKAADDPQRVTLAQEAQKAFRDDWAFIPWYSQAMSRWATKEVKGMEKNLDWQIAEPW
HHHHHCCCCCHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHCCCEEECCCE
NISIG
EECCC
>Mature Secondary Structure 
SLDQSDKTNLSRRNALKLGLAAGVGLTVFGMNARIVLADEGQVLKVAHPAFDQDWSPLR
CCCCCCCCCCHHHHHHEEHHHCCCCEEEEECCEEEEEECCCCEEEEECCCCCCCCCCCC
GGGRTFRWNSIWWASPMYFDSQGNIKPYVFTNWESADNTVWTFKIDPKAVFSDGSKITSA
CCCCEEEECCEEECCCEEECCCCCCCCEEEECCCCCCCEEEEEEECCHHEECCCCEEEEC
DVKGSWEVASMPNTKSQRADQVLSKVKGYAEIAAGSGNELTGVATPDEGTVVVTLAAADP
CCCCCEEEECCCCCHHHHHHHHHHHHCCHHEEECCCCCCEEEEECCCCCEEEEEEECCCH
IFFMRLANHIAPITKASQSRGSDGEEIIDWYKPENKPVFSGPFKLTSIDIDAGKITFEPN
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEECCCEEEEEEEECCCEEEECCC
EKFFGPKPKLARIDITSIEDNVTATSLIKSGEFNAHTELVTSTIIQDLGPEFSAGPLIPT
CCCCCCCCCEEEEEEECCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
SQHFWFNTSRAPMDDPKVRQALIMAVDRDGLFKASYPDGPHKKADQILNSVPGADNSGFE
CCCEEEECCCCCCCCHHHHHHHHEEECCCCCEECCCCCCCHHHHHHHHHHCCCCCCCCCC
PFPYDPAAAKKLLAESSYGGPERLPKLLFVGISAPAIQAAAQFIAEQWRQNLGITAVDMK
CCCCCHHHHHHHHHHCCCCCHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHCCEEEEECC
PQQDAYAGPDQNSVQIFRDDVGTRVPDAVSYLAGSIASTSSNAQNKLGGYKNDKVDSALA
CCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHH
EAATKAADDPQRVTLAQEAQKAFRDDWAFIPWYSQAMSRWATKEVKGMEKNLDWQIAEPW
HHHHHCCCCCHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHCCCEEECCCE
NISIG
EECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2821267; 3525163; 11677609; 8202710; 8747465; 10422831; 10438628 [H]