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The map label for this gene is rfbE [H]

Identifier: 190894632

GI number: 190894632

Start: 317435

End: 319477

Strand: Reverse

Name: rfbE [H]

Synonym: RHECIAT_PC0000295

Alternate gene names: 190894632

Gene position: 319477-317435 (Counterclockwise)

Preceding gene: 190894633

Following gene: 190894631

Centisome position: 29.27

GC content: 61.18

Gene sequence:

>2043_bases
ATGAACAGGACCGGTTCTCGAGGATCCAGCCAGCGGGAGACTCGGCGCTTCGGCTTCGTCGAATGGTTCAGACCGGGAGA
ACATGAGCGGGCCGAGACACTGCTGCCGGATATTCTGGCGAGCGGCGCCAGTTATCTCAGGACACATCTATCCTGGGCGG
AATATCTCGCGCCGGGCGGCCAGGAATGGTTCGATTGGCTGATCCCGCGCGTCGGCAGCGAAATCGATCTTCTGCCCTGC
ATCCACTACACGCCGCCATCCCTGTCGCGGACGGGCAAGTCCTCCGGCGCGCCGGTCGATCTCAAATCCTATGCCGACTT
CGTCGATCATATCCTGACCCGTTACGGCGGCTATTTTCGTCATATCGAACTCTGGAACGAGCCGAACAATCTGCTCGACT
GGGATTGGCGCCAGGACAGCGATTTTCTGCTCTTCTGCGAGATGATAGGCGCCGCCGCCTATTGGGCCAAGCAGCGAGGC
TACAAACCGGTGCTTGGCGGCCCGTGCCCCTTCGATCCCTACTGGCTCAACCTGATGGGCATACGCGGCGTGCTCGGTGT
CGTCGATGCCGTCGGCTTCCACGGCTTTCCCGGGACTTGGGATAGCGAAGAGGCGACCTGGGGCGGTTGGGACATGCATC
TGGGCGAGATGCGCGGGATTATCGATCGCTATAATGCCCATGCCGAGGTCTGGATTACCGAGGCCGGCTATTCCACATGG
CGCAATGACGAGATCGAGCAGGCGCGACGCTTCATGAAGGCGCTCACTGTCCCAGCCGACCGCATGTATTGGTATTCCTG
GCGCGACGTACCGCCGGATGTTCCGGTTCAGGAAGGCCTGTGGTTCGACCCGCGGCATTATCATCTCGGCGCGGTCACCC
ACGACAACAAGCCGAAGCTGCTTGCCCGTCTCCTGATGGAGGGAGGTGTTAACAGGCTGGAGGCGGTCGCAGCGCTTGCC
ACCCCTCGAATCGCCTCTGGTGCGGCGCCGATCGTTGTCACCGGCGGGAGCGGGTTTGTCGGCTGCAATTTGGCGGACAA
CTTGCTGAACGACGGGGAGGACGTCGTCATTCTGGATAGCCTGGCACGATCGGGCGTCGATCAGAACCTTTCCTGGCTGA
TAGAACGGCACGGGGAGCGGGTGCATCCGGTTCTTGCGGACGTTCGTGATCTGATGGGGATCGAAGCTGCCTTTTGTGAT
GCCAAGGCTGTCTTTCATTATGCTGCCCAGACGGCTGTGACCACGAGCCTCGTCGATCCGCTGGAGGATTTTGAAACGAA
TGCTCGCGGCACCCTCAATGTCCTGGAAGCGGTGCGCAAGGCGGGCAGGCGCGCGCCGGTCATTTTCGCCAGCACCAACA
AGGTCTATGGTGCTCTTGATGATCTTGGCATGATCGAACTCGACGATCGTTACCTTCCCGAGAGCGAAATCGTCAGGGAG
CAAGGTATCGGCGAGGACCGCCCCCTCGATTTCTGCACTCCCTACGGCTGCTCGAAAGGCGTTGCCGACCAATATATTCT
AGACTACGCCAAGTCCTTCGGAATACCGGCCGCGGTGCTGCGCATGAGCTGCATCTATGGGCCTCGCCAATTCGGCACCG
AGGACCAGGGCTGGGTCGCGCATTTCCTGATCCGGGCTCTCGGCGGAGAACCCATTTCCATCTACGGCGACGGTAAGCAG
GTGCGCGACATCCTCCACGTGAACGATGCCGTCGCCGCCTACCGGTCGTTGCTTGCGAATATTGATCGCGTCAGCGGCAA
TGCCTTCAATCTGGGCGGGGGGCCGCGTAACGCCGTCAGCGTGCTGGGGGTGCTGCGCGCGATCGAAGAGCTGACGGAAC
GTCCCGTTGCAACGAGCTTCGGCCCGTGGCGCGCCGGCGACCAGTTCTATTTCGTGGCCAACACGCAAAAGCTGGAACGC
GAAACCGGCTGGGAGGCCCGCATTGGATGGCGAGACGGCCTGCGGCATCTCGCCGAATGGCTGATTGTCAATCGTTTCGG
CGGTCGGCAGATCCCCAGGGAAAAGCGGAAGGTATCGGCATGA

Upstream 100 bases:

>100_bases
AAAATTCGCTCGGTCCTTTCGTCGACTGGGTCCGGCAAACGGGGGCGATCGACCGCGGCGCCGAGATGAAGCGGCAACTG
GAAGAACGGGGGCTGGTTTC

Downstream 100 bases:

>100_bases
TCCGACGGGAGCGACACAAGGGGCATCGCCGCGTGGTGATGACGGTCGATGCCGTGGGCGGCGTCTGGCGCTATGCCATG
GATCTCGCCGCGGCGCTGAG

Product: putative nucleoside diphosphate epimerase

Products: NA

Alternate protein names: CDP-tyvelose 2-epimerase [H]

Number of amino acids: Translated: 680; Mature: 680

Protein sequence:

>680_residues
MNRTGSRGSSQRETRRFGFVEWFRPGEHERAETLLPDILASGASYLRTHLSWAEYLAPGGQEWFDWLIPRVGSEIDLLPC
IHYTPPSLSRTGKSSGAPVDLKSYADFVDHILTRYGGYFRHIELWNEPNNLLDWDWRQDSDFLLFCEMIGAAAYWAKQRG
YKPVLGGPCPFDPYWLNLMGIRGVLGVVDAVGFHGFPGTWDSEEATWGGWDMHLGEMRGIIDRYNAHAEVWITEAGYSTW
RNDEIEQARRFMKALTVPADRMYWYSWRDVPPDVPVQEGLWFDPRHYHLGAVTHDNKPKLLARLLMEGGVNRLEAVAALA
TPRIASGAAPIVVTGGSGFVGCNLADNLLNDGEDVVILDSLARSGVDQNLSWLIERHGERVHPVLADVRDLMGIEAAFCD
AKAVFHYAAQTAVTTSLVDPLEDFETNARGTLNVLEAVRKAGRRAPVIFASTNKVYGALDDLGMIELDDRYLPESEIVRE
QGIGEDRPLDFCTPYGCSKGVADQYILDYAKSFGIPAAVLRMSCIYGPRQFGTEDQGWVAHFLIRALGGEPISIYGDGKQ
VRDILHVNDAVAAYRSLLANIDRVSGNAFNLGGGPRNAVSVLGVLRAIEELTERPVATSFGPWRAGDQFYFVANTQKLER
ETGWEARIGWRDGLRHLAEWLIVNRFGGRQIPREKRKVSA

Sequences:

>Translated_680_residues
MNRTGSRGSSQRETRRFGFVEWFRPGEHERAETLLPDILASGASYLRTHLSWAEYLAPGGQEWFDWLIPRVGSEIDLLPC
IHYTPPSLSRTGKSSGAPVDLKSYADFVDHILTRYGGYFRHIELWNEPNNLLDWDWRQDSDFLLFCEMIGAAAYWAKQRG
YKPVLGGPCPFDPYWLNLMGIRGVLGVVDAVGFHGFPGTWDSEEATWGGWDMHLGEMRGIIDRYNAHAEVWITEAGYSTW
RNDEIEQARRFMKALTVPADRMYWYSWRDVPPDVPVQEGLWFDPRHYHLGAVTHDNKPKLLARLLMEGGVNRLEAVAALA
TPRIASGAAPIVVTGGSGFVGCNLADNLLNDGEDVVILDSLARSGVDQNLSWLIERHGERVHPVLADVRDLMGIEAAFCD
AKAVFHYAAQTAVTTSLVDPLEDFETNARGTLNVLEAVRKAGRRAPVIFASTNKVYGALDDLGMIELDDRYLPESEIVRE
QGIGEDRPLDFCTPYGCSKGVADQYILDYAKSFGIPAAVLRMSCIYGPRQFGTEDQGWVAHFLIRALGGEPISIYGDGKQ
VRDILHVNDAVAAYRSLLANIDRVSGNAFNLGGGPRNAVSVLGVLRAIEELTERPVATSFGPWRAGDQFYFVANTQKLER
ETGWEARIGWRDGLRHLAEWLIVNRFGGRQIPREKRKVSA
>Mature_680_residues
MNRTGSRGSSQRETRRFGFVEWFRPGEHERAETLLPDILASGASYLRTHLSWAEYLAPGGQEWFDWLIPRVGSEIDLLPC
IHYTPPSLSRTGKSSGAPVDLKSYADFVDHILTRYGGYFRHIELWNEPNNLLDWDWRQDSDFLLFCEMIGAAAYWAKQRG
YKPVLGGPCPFDPYWLNLMGIRGVLGVVDAVGFHGFPGTWDSEEATWGGWDMHLGEMRGIIDRYNAHAEVWITEAGYSTW
RNDEIEQARRFMKALTVPADRMYWYSWRDVPPDVPVQEGLWFDPRHYHLGAVTHDNKPKLLARLLMEGGVNRLEAVAALA
TPRIASGAAPIVVTGGSGFVGCNLADNLLNDGEDVVILDSLARSGVDQNLSWLIERHGERVHPVLADVRDLMGIEAAFCD
AKAVFHYAAQTAVTTSLVDPLEDFETNARGTLNVLEAVRKAGRRAPVIFASTNKVYGALDDLGMIELDDRYLPESEIVRE
QGIGEDRPLDFCTPYGCSKGVADQYILDYAKSFGIPAAVLRMSCIYGPRQFGTEDQGWVAHFLIRALGGEPISIYGDGKQ
VRDILHVNDAVAAYRSLLANIDRVSGNAFNLGGGPRNAVSVLGVLRAIEELTERPVATSFGPWRAGDQFYFVANTQKLER
ETGWEARIGWRDGLRHLAEWLIVNRFGGRQIPREKRKVSA

Specific function: Catalyzes the isomeration of CDP-paratose to CDP- tyvelose [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=325, Percent_Identity=27.6923076923077, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI7657641, Length=343, Percent_Identity=24.7813411078717, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI56237023, Length=364, Percent_Identity=26.9230769230769, Blast_Score=89, Evalue=2e-17,
Organism=Homo sapiens, GI56118217, Length=364, Percent_Identity=26.9230769230769, Blast_Score=89, Evalue=2e-17,
Organism=Homo sapiens, GI189083684, Length=364, Percent_Identity=26.9230769230769, Blast_Score=89, Evalue=2e-17,
Organism=Escherichia coli, GI48994969, Length=364, Percent_Identity=27.1978021978022, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788353, Length=360, Percent_Identity=26.3888888888889, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786974, Length=334, Percent_Identity=30.2395209580838, Blast_Score=108, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI115532424, Length=339, Percent_Identity=28.023598820059, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17539532, Length=254, Percent_Identity=30.3149606299213, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71982035, Length=364, Percent_Identity=26.9230769230769, Blast_Score=89, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI71982038, Length=367, Percent_Identity=27.2479564032698, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17568069, Length=344, Percent_Identity=23.8372093023256, Blast_Score=85, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319493, Length=367, Percent_Identity=25.8855585831063, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21356223, Length=338, Percent_Identity=27.5147928994083, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI19923002, Length=345, Percent_Identity=27.8260869565217, Blast_Score=92, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.10 [H]

Molecular weight: Translated: 76048; Mature: 76048

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRTGSRGSSQRETRRFGFVEWFRPGEHERAETLLPDILASGASYLRTHLSWAEYLAPGG
CCCCCCCCCCHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
QEWFDWLIPRVGSEIDLLPCIHYTPPSLSRTGKSSGAPVDLKSYADFVDHILTRYGGYFR
HHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEE
HIELWNEPNNLLDWDWRQDSDFLLFCEMIGAAAYWAKQRGYKPVLGGPCPFDPYWLNLMG
EEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
IRGVLGVVDAVGFHGFPGTWDSEEATWGGWDMHLGEMRGIIDRYNAHAEVWITEAGYSTW
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCC
RNDEIEQARRFMKALTVPADRMYWYSWRDVPPDVPVQEGLWFDPRHYHLGAVTHDNKPKL
CCCHHHHHHHHHHHHCCCCHHEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHH
LARLLMEGGVNRLEAVAALATPRIASGAAPIVVTGGSGFVGCNLADNLLNDGEDVVILDS
HHHHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCEEECCHHHHHHCCCCCEEEEEH
LARSGVDQNLSWLIERHGERVHPVLADVRDLMGIEAAFCDAKAVFHYAAQTAVTTSLVDP
HHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEDFETNARGTLNVLEAVRKAGRRAPVIFASTNKVYGALDDLGMIELDDRYLPESEIVRE
HHHHCCCCCCHHHHHHHHHHCCCCCCEEEEECCCHHCCCCCCCEEEECCCCCCHHHHHHH
QGIGEDRPLDFCTPYGCSKGVADQYILDYAKSFGIPAAVLRMSCIYGPRQFGTEDQGWVA
HCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHCCCCCCHHHH
HFLIRALGGEPISIYGDGKQVRDILHVNDAVAAYRSLLANIDRVSGNAFNLGGGPRNAVS
HHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHH
VLGVLRAIEELTERPVATSFGPWRAGDQFYFVANTQKLERETGWEARIGWRDGLRHLAEW
HHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCHHHHHHCCCCEEECHHHHHHHHHHH
LIVNRFGGRQIPREKRKVSA
HHHHCCCCCCCCHHHHCCCC
>Mature Secondary Structure
MNRTGSRGSSQRETRRFGFVEWFRPGEHERAETLLPDILASGASYLRTHLSWAEYLAPGG
CCCCCCCCCCHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
QEWFDWLIPRVGSEIDLLPCIHYTPPSLSRTGKSSGAPVDLKSYADFVDHILTRYGGYFR
HHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEE
HIELWNEPNNLLDWDWRQDSDFLLFCEMIGAAAYWAKQRGYKPVLGGPCPFDPYWLNLMG
EEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
IRGVLGVVDAVGFHGFPGTWDSEEATWGGWDMHLGEMRGIIDRYNAHAEVWITEAGYSTW
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCC
RNDEIEQARRFMKALTVPADRMYWYSWRDVPPDVPVQEGLWFDPRHYHLGAVTHDNKPKL
CCCHHHHHHHHHHHHCCCCHHEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHH
LARLLMEGGVNRLEAVAALATPRIASGAAPIVVTGGSGFVGCNLADNLLNDGEDVVILDS
HHHHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCEEECCHHHHHHCCCCCEEEEEH
LARSGVDQNLSWLIERHGERVHPVLADVRDLMGIEAAFCDAKAVFHYAAQTAVTTSLVDP
HHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEDFETNARGTLNVLEAVRKAGRRAPVIFASTNKVYGALDDLGMIELDDRYLPESEIVRE
HHHHCCCCCCHHHHHHHHHHCCCCCCEEEEECCCHHCCCCCCCEEEECCCCCCHHHHHHH
QGIGEDRPLDFCTPYGCSKGVADQYILDYAKSFGIPAAVLRMSCIYGPRQFGTEDQGWVA
HCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHCCCCCCHHHH
HFLIRALGGEPISIYGDGKQVRDILHVNDAVAAYRSLLANIDRVSGNAFNLGGGPRNAVS
HHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHH
VLGVLRAIEELTERPVATSFGPWRAGDQFYFVANTQKLERETGWEARIGWRDGLRHLAEW
HHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCHHHHHHCCCCEEECHHHHHHHHHHH
LIVNRFGGRQIPREKRKVSA
HHHHCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2793833; 11677608; 12644504; 12642575 [H]