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The map label for this gene is cmoB [H]

Identifier: 190894626

GI number: 190894626

Start: 311197

End: 311931

Strand: Reverse

Name: cmoB [H]

Synonym: RHECIAT_PC0000289

Alternate gene names: 190894626

Gene position: 311931-311197 (Counterclockwise)

Preceding gene: 190894627

Following gene: 190894625

Centisome position: 28.58

GC content: 56.73

Gene sequence:

>735_bases
ATGATGAACCCGTCTTTGAAACGGCGTATCAAAGAATTGGGCCCCTGGTTCCATAATATGCGGCTGGGGGAGACCGAGAC
CGCGCCGGACCATTTTCTCGGCGACTACCCGGCATTCAAATGGGACGGTTTCAAACATGTGGTGCCTGACGATCTTGCAG
GCCGCAGCGTTCTTGATATCGGCTGTAATGCCGGATTTTATACCCTCGAGATGAAACGCCGCAACGCGGGCCGGGTTCTC
GGCATCGATAGTGATCCACGCTATCTCGAACAGGCCCGTTTCGCCGCCGATCATTTTGCCCTCGACATCGAGTTCAGGCA
GATGTCGGTTTATGAGGTTGCACAACTCGGCGAAACCTTCGATCTCGTGCTCTTCATGGGTGTCCTTTACCACCTGCGCC
ATCCGTTGCTGGCACTTGATCTGCTCTACGAACACGTGGTTGGCGATCTCATGCTGTTCCAATGTCTGCAGCGGGGCGAG
GAGCGGATTGCCCCGCTTCAAGATGACTATGAATTTTCGGAATGGAACGTTTTCGACCGGCGGGACTACCCGACGCTGTT
CTTCGTCGAAGAGCGCTATGCGGCCGATCCTACCAACTGGTTCATCCCGAACAAGGCAGCGGTCGAAGCCATGTTGCGAA
GCTCCGGATTTGTGTTGGAGGCAAATCCCGAGCGCGAGGTTTATCTTTGCCGCCGAGGCCGGCGGCCCTACATGGTCGAG
CCGCCGCCCGCTTGA

Upstream 100 bases:

>100_bases
CGATCGCCCACGCAAGCGAACAGGAGCGCCTCGCTCTCGTCGCAGAATGGCGGCCTCGCAGGCCGCCTCCCTGGAGAGCT
GATCGAAAGGCGGATGCACG

Downstream 100 bases:

>100_bases
GGCGATCGCTCGACAGGACAATGCAGGTCCACAATGTTGAAAGGTACGGGACCGTTTTCGTTTGCCATTGTTGCGATCCT
CGTCATCGCGACCTTGGTCT

Product: putative methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MMNPSLKRRIKELGPWFHNMRLGETETAPDHFLGDYPAFKWDGFKHVVPDDLAGRSVLDIGCNAGFYTLEMKRRNAGRVL
GIDSDPRYLEQARFAADHFALDIEFRQMSVYEVAQLGETFDLVLFMGVLYHLRHPLLALDLLYEHVVGDLMLFQCLQRGE
ERIAPLQDDYEFSEWNVFDRRDYPTLFFVEERYAADPTNWFIPNKAAVEAMLRSSGFVLEANPEREVYLCRRGRRPYMVE
PPPA

Sequences:

>Translated_244_residues
MMNPSLKRRIKELGPWFHNMRLGETETAPDHFLGDYPAFKWDGFKHVVPDDLAGRSVLDIGCNAGFYTLEMKRRNAGRVL
GIDSDPRYLEQARFAADHFALDIEFRQMSVYEVAQLGETFDLVLFMGVLYHLRHPLLALDLLYEHVVGDLMLFQCLQRGE
ERIAPLQDDYEFSEWNVFDRRDYPTLFFVEERYAADPTNWFIPNKAAVEAMLRSSGFVLEANPEREVYLCRRGRRPYMVE
PPPA
>Mature_244_residues
MMNPSLKRRIKELGPWFHNMRLGETETAPDHFLGDYPAFKWDGFKHVVPDDLAGRSVLDIGCNAGFYTLEMKRRNAGRVL
GIDSDPRYLEQARFAADHFALDIEFRQMSVYEVAQLGETFDLVLFMGVLYHLRHPLLALDLLYEHVVGDLMLFQCLQRGE
ERIAPLQDDYEFSEWNVFDRRDYPTLFFVEERYAADPTNWFIPNKAAVEAMLRSSGFVLEANPEREVYLCRRGRRPYMVE
PPPA

Specific function: Catalyzes the conversion of 5-hydroxyuridine (ho5U) to 5-methoxyuridine (mo5U) at position 34 in tRNA [H]

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. CmoB family [H]

Homologues:

Organism=Escherichia coli, GI1788178, Length=188, Percent_Identity=30.8510638297872, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010017 [H]

Pfam domain/function: PF08003 Methyltransf_9 [H]

EC number: NA

Molecular weight: Translated: 28461; Mature: 28461

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNPSLKRRIKELGPWFHNMRLGETETAPDHFLGDYPAFKWDGFKHVVPDDLAGRSVLDI
CCCHHHHHHHHHHCHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEE
GCNAGFYTLEMKRRNAGRVLGIDSDPRYLEQARFAADHFALDIEFRQMSVYEVAQLGETF
CCCCCEEEEEEECCCCCCEEECCCCHHHHHHHHHHHHHEEEEEEHHHHHHHHHHHCCCHH
DLVLFMGVLYHLRHPLLALDLLYEHVVGDLMLFQCLQRGEERIAPLQDDYEFSEWNVFDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
RDYPTLFFVEERYAADPTNWFIPNKAAVEAMLRSSGFVLEANPEREVYLCRRGRRPYMVE
CCCCEEEEEECCCCCCCCCEECCCHHHHHHHHHHCCEEEEECCCCEEHEEECCCCCCCCC
PPPA
CCCC
>Mature Secondary Structure
MMNPSLKRRIKELGPWFHNMRLGETETAPDHFLGDYPAFKWDGFKHVVPDDLAGRSVLDI
CCCHHHHHHHHHHCHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEE
GCNAGFYTLEMKRRNAGRVLGIDSDPRYLEQARFAADHFALDIEFRQMSVYEVAQLGETF
CCCCCEEEEEEECCCCCCEEECCCCHHHHHHHHHHHHHEEEEEEHHHHHHHHHHHCCCHH
DLVLFMGVLYHLRHPLLALDLLYEHVVGDLMLFQCLQRGEERIAPLQDDYEFSEWNVFDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
RDYPTLFFVEERYAADPTNWFIPNKAAVEAMLRSSGFVLEANPEREVYLCRRGRRPYMVE
CCCCEEEEEECCCCCCCCCEECCCHHHHHHHHHHCCEEEEECCCCEEHEEECCCCCCCCC
PPPA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA