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The map label for this gene is rutB [H]

Identifier: 190894424

GI number: 190894424

Start: 81682

End: 82350

Strand: Reverse

Name: rutB [H]

Synonym: RHECIAT_PC0000086

Alternate gene names: 190894424

Gene position: 82350-81682 (Counterclockwise)

Preceding gene: 190894425

Following gene: 190894423

Centisome position: 7.54

GC content: 62.63

Gene sequence:

>669_bases
ATGGCCGAGATCAAGGCAGAACCCTTCGCTTTCCCGGTAAAGCACGATCAGCTCGCCCTTATTGTCATCGATATGCAGCG
CGATTTTGCAGAGCCCGGCGGCTTCGGCGCAAGCCTCGGCAACGACGTCAGCCGCATCGGCAGGATCGTCCCAGATGTCA
AACGCCTGATCCAGGGCTTCCGATATGCCGGGCTGCCGGTCATCCATACGATGGAATGCCACAGACCCGACCTTTCGGAT
CTGCCGCCGTCAAAACGCGACCGCGGCAATCCCATGCTCAGGATCGGCGACGAAGGCCCCATGGGCCGCATCCTCATAGC
TGGCGAGCCGGGAACGGCGATCCTACCGGAGCTTGCTCCGATCGATGGCGAAGTGGTCATCGAAAAGCCCGGCAAGGGCG
CCTTCTATGCCACCGGACTCGCGGAGGCGCTGCAGCGAAAGGGCATCAGGCAACTGGTCTTTGCTGGCGTGACCACCGAG
GTCTGCGTTCAGACGACCATGCGCGAGGCGAACGACCGCGGCTATGAGTGCTTGCTCGCCGAGGAGGCGACGGAAAGCTA
CTTTCCCGAGTTCAAGGCCGCCGCCATCGCCATGATCCGCGCCCAGGGCGCGATCGTCGGCTGGACCGCCCATGTCGACG
ACATTCTGGAAAGCATTGCCCATGCCTGA

Upstream 100 bases:

>100_bases
ACCGCATCATGGTCATTTCCGAAGGCAGGCTGGTTTACGAGACACCGGCGCGTGCGGCCGATATCGCGGTCATCGGCGCC
CACATGGCGGGGCATCATTG

Downstream 100 bases:

>100_bases
AACCACCCGCGAACTTATGACATCCGGCGGCTGGAAGGAATTGGCATTCGGCCCCTTCCGCGATCAGGTGACCATCCATT
GGATCCGTCCCTTCGAGGAC

Product: putative isochorismatase hydrolase

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MAEIKAEPFAFPVKHDQLALIVIDMQRDFAEPGGFGASLGNDVSRIGRIVPDVKRLIQGFRYAGLPVIHTMECHRPDLSD
LPPSKRDRGNPMLRIGDEGPMGRILIAGEPGTAILPELAPIDGEVVIEKPGKGAFYATGLAEALQRKGIRQLVFAGVTTE
VCVQTTMREANDRGYECLLAEEATESYFPEFKAAAIAMIRAQGAIVGWTAHVDDILESIAHA

Sequences:

>Translated_222_residues
MAEIKAEPFAFPVKHDQLALIVIDMQRDFAEPGGFGASLGNDVSRIGRIVPDVKRLIQGFRYAGLPVIHTMECHRPDLSD
LPPSKRDRGNPMLRIGDEGPMGRILIAGEPGTAILPELAPIDGEVVIEKPGKGAFYATGLAEALQRKGIRQLVFAGVTTE
VCVQTTMREANDRGYECLLAEEATESYFPEFKAAAIAMIRAQGAIVGWTAHVDDILESIAHA
>Mature_221_residues
AEIKAEPFAFPVKHDQLALIVIDMQRDFAEPGGFGASLGNDVSRIGRIVPDVKRLIQGFRYAGLPVIHTMECHRPDLSDL
PPSKRDRGNPMLRIGDEGPMGRILIAGEPGTAILPELAPIDGEVVIEKPGKGAFYATGLAEALQRKGIRQLVFAGVTTEV
CVQTTMREANDRGYECLLAEEATESYFPEFKAAAIAMIRAQGAIVGWTAHVDDILESIAHA

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=232, Percent_Identity=29.3103448275862, Blast_Score=100, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 24048; Mature: 23917

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEIKAEPFAFPVKHDQLALIVIDMQRDFAEPGGFGASLGNDVSRIGRIVPDVKRLIQGF
CCCCCCCCCCCCCCCCCEEEEEEECCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RYAGLPVIHTMECHRPDLSDLPPSKRDRGNPMLRIGDEGPMGRILIAGEPGTAILPELAP
HCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCCCCEECCCCCC
IDGEVVIEKPGKGAFYATGLAEALQRKGIRQLVFAGVTTEVCVQTTMREANDRGYECLLA
CCCEEEEECCCCCCEEHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEE
EEATESYFPEFKAAAIAMIRAQGAIVGWTAHVDDILESIAHA
CHHHHHHCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCC
>Mature Secondary Structure 
AEIKAEPFAFPVKHDQLALIVIDMQRDFAEPGGFGASLGNDVSRIGRIVPDVKRLIQGF
CCCCCCCCCCCCCCCCEEEEEEECCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RYAGLPVIHTMECHRPDLSDLPPSKRDRGNPMLRIGDEGPMGRILIAGEPGTAILPELAP
HCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCCCCEECCCCCC
IDGEVVIEKPGKGAFYATGLAEALQRKGIRQLVFAGVTTEVCVQTTMREANDRGYECLLA
CCCEEEEECCCCCCEEHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEE
EEATESYFPEFKAAAIAMIRAQGAIVGWTAHVDDILESIAHA
CHHHHHHCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA