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The map label for this gene is yafC [H]

Identifier: 190894416

GI number: 190894416

Start: 73444

End: 74343

Strand: Reverse

Name: yafC [H]

Synonym: RHECIAT_PC0000078

Alternate gene names: 190894416

Gene position: 74343-73444 (Counterclockwise)

Preceding gene: 190894423

Following gene: 190894411

Centisome position: 6.81

GC content: 62.44

Gene sequence:

>900_bases
ATGAACGACTATAAAGCTCTCCGCACTTTCCTCCTCGCCGCTGAGAAGCGGAACTTCGCGCAAGTCGCGCGCGAACTCGA
CATGACGCCTGCGGCCGTAACGCGAGCCATCGCTGCCCTTGAGGCGGAGCTCGGCGTCCAGCTCTTCGTGCGCACCACGA
GGCAGGTGTCCCTGACGACGGACGGCGCGATCTTCGCGGCGCAGGTCCAGCCCGCGGTGAAGACGCTCGAGGACGCGCGG
CGCGAGATGATGAACGCGCACAAGGCTGAGGAGGGGCGCCTCAGGATCAGCGCGCCAACCTGGTTTGGAAAGGTCGTGCT
GCCGCCGATCCTGTCCGGCTTCAAGGAGAAGTATCCGAAGATGAGCTTCGAGATCTCCCTCTCTGATGGGCTGGTGAACA
TCGTTGACGACGACTACGATCTGGCGATCCGCATCTCCTCCCAGCCGTCCGATAAGTTCACCATCTGGAGAAAGATCCGC
GTCGTGCCGCGCATTCTCGTCGCCGCGCCCGGCAGCCGCTTCGTCGACATGTCGCATCCTCACGAGCTGACGCCGGATGA
CTGCCTTGCCTATAGCGGGGAAAGCCGGCGGGAGAACTGGATCCTGTCGGATGGCGGATCGAGCATTGCGATCTCTGCCG
GACGGGCGTTCAGTGCGAACAGTGGCGAGGTTCTGGCCGACATGGCCGCAGATGGAGCGGGCGTCGCCTTGTTGCCGGGG
TTCAATATTGCCGAGCACATCCGTAGCGGTCGCCTTGTTCACGTCTTTCGTGGATGGGCGCCGCCGGATCTATGGCTGAC
GCTCTATTATCCACCATACCAAGCGCTACCGCCGCGGATCGCCTCGTTCTCCAAGTTCTTCGAGGAGCAGGTGGCAGCGC
ACATGGTCATGCTTGATTGA

Upstream 100 bases:

>100_bases
GCACTGGTCTTTGTTGTGTGAACTGAATGTAGACCGGTTGCTCACAGCGCTGAATTGCAATACTTCTTTCGACAGAATTG
CAAGAAGTGAAATAATAGCC

Downstream 100 bases:

>100_bases
CAGGCAGTGGACGGTCAACGTCGGCGCTTGATCGACTGTTTACAGTCATTGCTTGCAGCCTTGCGGCGACAAGCTTTTGC
AACTGCCACAGGTTCCGATC

Product: putative LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MNDYKALRTFLLAAEKRNFAQVARELDMTPAAVTRAIAALEAELGVQLFVRTTRQVSLTTDGAIFAAQVQPAVKTLEDAR
REMMNAHKAEEGRLRISAPTWFGKVVLPPILSGFKEKYPKMSFEISLSDGLVNIVDDDYDLAIRISSQPSDKFTIWRKIR
VVPRILVAAPGSRFVDMSHPHELTPDDCLAYSGESRRENWILSDGGSSIAISAGRAFSANSGEVLADMAADGAGVALLPG
FNIAEHIRSGRLVHVFRGWAPPDLWLTLYYPPYQALPPRIASFSKFFEEQVAAHMVMLD

Sequences:

>Translated_299_residues
MNDYKALRTFLLAAEKRNFAQVARELDMTPAAVTRAIAALEAELGVQLFVRTTRQVSLTTDGAIFAAQVQPAVKTLEDAR
REMMNAHKAEEGRLRISAPTWFGKVVLPPILSGFKEKYPKMSFEISLSDGLVNIVDDDYDLAIRISSQPSDKFTIWRKIR
VVPRILVAAPGSRFVDMSHPHELTPDDCLAYSGESRRENWILSDGGSSIAISAGRAFSANSGEVLADMAADGAGVALLPG
FNIAEHIRSGRLVHVFRGWAPPDLWLTLYYPPYQALPPRIASFSKFFEEQVAAHMVMLD
>Mature_299_residues
MNDYKALRTFLLAAEKRNFAQVARELDMTPAAVTRAIAALEAELGVQLFVRTTRQVSLTTDGAIFAAQVQPAVKTLEDAR
REMMNAHKAEEGRLRISAPTWFGKVVLPPILSGFKEKYPKMSFEISLSDGLVNIVDDDYDLAIRISSQPSDKFTIWRKIR
VVPRILVAAPGSRFVDMSHPHELTPDDCLAYSGESRRENWILSDGGSSIAISAGRAFSANSGEVLADMAADGAGVALLPG
FNIAEHIRSGRLVHVFRGWAPPDLWLTLYYPPYQALPPRIASFSKFFEEQVAAHMVMLD

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=293, Percent_Identity=29.3515358361775, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI87081978, Length=299, Percent_Identity=30.7692307692308, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI145693193, Length=303, Percent_Identity=27.3927392739274, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1787128, Length=290, Percent_Identity=27.9310344827586, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1789440, Length=281, Percent_Identity=26.3345195729537, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787589, Length=274, Percent_Identity=27.007299270073, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1788706, Length=302, Percent_Identity=28.1456953642384, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1789639, Length=256, Percent_Identity=25.78125, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790783, Length=256, Percent_Identity=25.78125, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790208, Length=251, Percent_Identity=29.4820717131474, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789173, Length=297, Percent_Identity=28.956228956229, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786448, Length=138, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87082132, Length=298, Percent_Identity=22.4832214765101, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI145693105, Length=150, Percent_Identity=26, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787879, Length=151, Percent_Identity=29.8013245033113, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33064; Mature: 33064

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDYKALRTFLLAAEKRNFAQVARELDMTPAAVTRAIAALEAELGVQLFVRTTRQVSLTT
CCHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEEECCEEEEEC
DGAIFAAQVQPAVKTLEDARREMMNAHKAEEGRLRISAPTWFGKVVLPPILSGFKEKYPK
CCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCC
MSFEISLSDGLVNIVDDDYDLAIRISSQPSDKFTIWRKIRVVPRILVAAPGSRFVDMSHP
EEEEEEECCCEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHEEECCCCCEEECCCC
HELTPDDCLAYSGESRRENWILSDGGSSIAISAGRAFSANSGEVLADMAADGAGVALLPG
CCCCCHHHHCCCCCCCCCCEEEECCCCEEEEECCCEECCCCCCEEEECCCCCCCEEEECC
FNIAEHIRSGRLVHVFRGWAPPDLWLTLYYPPYQALPPRIASFSKFFEEQVAAHMVMLD
CCHHHHHCCCCEEEEEECCCCCCEEEEEEECCHHCCCHHHHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure
MNDYKALRTFLLAAEKRNFAQVARELDMTPAAVTRAIAALEAELGVQLFVRTTRQVSLTT
CCHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEEECCEEEEEC
DGAIFAAQVQPAVKTLEDARREMMNAHKAEEGRLRISAPTWFGKVVLPPILSGFKEKYPK
CCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCC
MSFEISLSDGLVNIVDDDYDLAIRISSQPSDKFTIWRKIRVVPRILVAAPGSRFVDMSHP
EEEEEEECCCEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHEEECCCCCEEECCCC
HELTPDDCLAYSGESRRENWILSDGGSSIAISAGRAFSANSGEVLADMAADGAGVALLPG
CCCCCHHHHCCCCCCCCCCEEEECCCCEEEEECCCEECCCCCCEEEECCCCCCCEEEECC
FNIAEHIRSGRLVHVFRGWAPPDLWLTLYYPPYQALPPRIASFSKFFEEQVAAHMVMLD
CCHHHHHCCCCEEEEEECCCCCCEEEEEEECCHHCCCHHHHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]