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The map label for this gene is lpdA [H]

Identifier: 190571293

GI number: 190571293

Start: 963371

End: 964756

Strand: Direct

Name: lpdA [H]

Synonym: WPa_0896

Alternate gene names: 190571293

Gene position: 963371-964756 (Clockwise)

Preceding gene: 190571292

Following gene: 190571295

Centisome position: 64.98

GC content: 35.93

Gene sequence:

>1386_bases
ATGAATGAGTATGATATTACAGTTATAGGCAGCGGTCCTGGTGGTTATATAGCAGCAATTAGGGCGGCGCAGCTTGGGTT
TAAAACTGCAATTGTTGAAAAGGAGGAAAATTTGGGTGGTATATGCTTAAATTGGGGATGTATACCAACAAAATCGCTAC
TTAGAGCGTCTGAAGTTTATAGATTAATAAGAAGATCAGAAGAATTTGGCATAAAAGTAAAGGATGCAAGTTTTAATATA
CAATCAATGGTGAAATATTCAAGGAACGTTGTTGGCAAGCTATCAAGTGGTGTTGAGTATTTGATGAAAAAAAATAACAT
CAAAGTCCATCAAGGCTTCGGTAAACTTGCAGGTAATCGTACTATAAAAATTCTTAATGACAAAAAGGAGGAGGAAATTT
CTTCCAAGCATATCATTTTAGCAACAGGTGTAAGGGCAAGAAATCTTCCTGGAATCGAAGTAGATGGAGATTTAATATGG
AATGCGCAGCACGCTATGACTCCAAAGAAGTTACCAAAATCTCTACTAATCATAGGTTCTGGTGCGATAGGAATAGAGTT
TGCAAGTTTTTATAGTACTTTGGGAGTTGATGTAACAATTATAGAAGTAAAAGACACTATTTTGCCGCTGGAAGATAAAG
ATATTTCAAATTTAGCACAAGAAATATTCACAAAACAAAGGATAAAAATATATACAAACAGTAGCGTAAAAGCTTTGACT
AAAAATAAAGACTTTGCTCAAGTGTTACTAAGTAGTGGTGAGAGCAAAGAATTTGATAGGGTGATTGTTGCAGTTGGCGT
TCAAGCAAACACTGAAAATATAGGTTTAGAAAATACAAAAATTAAGTTGAGTTCTTCTGGTTTCATTGAAACGAATGAGT
GGTATGAAACAAGTGAATCAAGTGTGTATGCAATAGGTGATGTAGCTGGTCCCCCATGTTTAGCGCATAAAGCAAGTCAT
GAAGCTGTAATCTGTGTTGAGAAAATTGCCGGTAAAAATGCTCATAAGTTAAAAAAAGAGTGCATACCAAATTGCACTTA
CTCTCATCCGCAAGTAGCAAGCGTTGGCCTTACTGAGGAACAGGCAATAAAAAGTGGATACGATATAAAAGTAGGAAAAT
TTCACTCCAACTTTAATGGTAAATCTATTGCACTAAGTGAAACTGAAGGTTTAGTGAAAACAATTATAGACAAAAAAACA
GGCGAACTTCTGGGTAGCCACATGATAGGTGCGGAAGTAACAGAGTTAATTAGCAATTTTGCTCTTGCAAAGCAACTAGA
AGGAACAGACTTCGACATAAAATCTACGATTTTTCCTCATCCAACCATTTCAGAGATGATACATGAGTCAGTACTTGCTG
CAGATGGTGAATCGCTGAATAGTTGA

Upstream 100 bases:

>100_bases
AGGAACGTGGGAAGGTTTGTCAAGTCGCTTCTATACACCTGAGCATTGATCATACGGGTTCCATCTGTTATATATAGTCT
TTTGCATACGAAGAAAACTG

Downstream 100 bases:

>100_bases
ACTAACGTTCTATCGTTTTGGAGCTACAAGTATCCTGATTAGGCGATTCAATTCTAATAGATTGATTACCAATATCAGAT
TTTTCTAACTTTTCTTTTAA

Product: lipoamide dehydrogenase, pyruvate dehydrogenase complex, E3 component

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MNEYDITVIGSGPGGYIAAIRAAQLGFKTAIVEKEENLGGICLNWGCIPTKSLLRASEVYRLIRRSEEFGIKVKDASFNI
QSMVKYSRNVVGKLSSGVEYLMKKNNIKVHQGFGKLAGNRTIKILNDKKEEEISSKHIILATGVRARNLPGIEVDGDLIW
NAQHAMTPKKLPKSLLIIGSGAIGIEFASFYSTLGVDVTIIEVKDTILPLEDKDISNLAQEIFTKQRIKIYTNSSVKALT
KNKDFAQVLLSSGESKEFDRVIVAVGVQANTENIGLENTKIKLSSSGFIETNEWYETSESSVYAIGDVAGPPCLAHKASH
EAVICVEKIAGKNAHKLKKECIPNCTYSHPQVASVGLTEEQAIKSGYDIKVGKFHSNFNGKSIALSETEGLVKTIIDKKT
GELLGSHMIGAEVTELISNFALAKQLEGTDFDIKSTIFPHPTISEMIHESVLAADGESLNS

Sequences:

>Translated_461_residues
MNEYDITVIGSGPGGYIAAIRAAQLGFKTAIVEKEENLGGICLNWGCIPTKSLLRASEVYRLIRRSEEFGIKVKDASFNI
QSMVKYSRNVVGKLSSGVEYLMKKNNIKVHQGFGKLAGNRTIKILNDKKEEEISSKHIILATGVRARNLPGIEVDGDLIW
NAQHAMTPKKLPKSLLIIGSGAIGIEFASFYSTLGVDVTIIEVKDTILPLEDKDISNLAQEIFTKQRIKIYTNSSVKALT
KNKDFAQVLLSSGESKEFDRVIVAVGVQANTENIGLENTKIKLSSSGFIETNEWYETSESSVYAIGDVAGPPCLAHKASH
EAVICVEKIAGKNAHKLKKECIPNCTYSHPQVASVGLTEEQAIKSGYDIKVGKFHSNFNGKSIALSETEGLVKTIIDKKT
GELLGSHMIGAEVTELISNFALAKQLEGTDFDIKSTIFPHPTISEMIHESVLAADGESLNS
>Mature_461_residues
MNEYDITVIGSGPGGYIAAIRAAQLGFKTAIVEKEENLGGICLNWGCIPTKSLLRASEVYRLIRRSEEFGIKVKDASFNI
QSMVKYSRNVVGKLSSGVEYLMKKNNIKVHQGFGKLAGNRTIKILNDKKEEEISSKHIILATGVRARNLPGIEVDGDLIW
NAQHAMTPKKLPKSLLIIGSGAIGIEFASFYSTLGVDVTIIEVKDTILPLEDKDISNLAQEIFTKQRIKIYTNSSVKALT
KNKDFAQVLLSSGESKEFDRVIVAVGVQANTENIGLENTKIKLSSSGFIETNEWYETSESSVYAIGDVAGPPCLAHKASH
EAVICVEKIAGKNAHKLKKECIPNCTYSHPQVASVGLTEEQAIKSGYDIKVGKFHSNFNGKSIALSETEGLVKTIIDKKT
GELLGSHMIGAEVTELISNFALAKQLEGTDFDIKSTIFPHPTISEMIHESVLAADGESLNS

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=470, Percent_Identity=38.936170212766, Blast_Score=316, Evalue=2e-86,
Organism=Homo sapiens, GI50301238, Length=468, Percent_Identity=27.1367521367521, Blast_Score=142, Evalue=9e-34,
Organism=Homo sapiens, GI33519430, Length=453, Percent_Identity=25.60706401766, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI33519428, Length=453, Percent_Identity=25.60706401766, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI33519426, Length=453, Percent_Identity=25.60706401766, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI148277071, Length=453, Percent_Identity=25.60706401766, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI148277065, Length=453, Percent_Identity=25.60706401766, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI22035672, Length=478, Percent_Identity=25.9414225941423, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI291045266, Length=427, Percent_Identity=25.5269320843091, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI291045268, Length=423, Percent_Identity=24.113475177305, Blast_Score=101, Evalue=2e-21,
Organism=Escherichia coli, GI1786307, Length=458, Percent_Identity=33.6244541484716, Blast_Score=268, Evalue=7e-73,
Organism=Escherichia coli, GI87082354, Length=451, Percent_Identity=27.0509977827051, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1789915, Length=434, Percent_Identity=28.5714285714286, Blast_Score=177, Evalue=9e-46,
Organism=Escherichia coli, GI87081717, Length=458, Percent_Identity=27.7292576419214, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1788892, Length=218, Percent_Identity=25.6880733944954, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI32565766, Length=469, Percent_Identity=37.1002132196162, Blast_Score=305, Evalue=5e-83,
Organism=Caenorhabditis elegans, GI17557007, Length=476, Percent_Identity=25.6302521008403, Blast_Score=138, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI71983429, Length=441, Percent_Identity=24.7165532879819, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71983419, Length=441, Percent_Identity=24.7165532879819, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71982272, Length=484, Percent_Identity=24.7933884297521, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17559934, Length=229, Percent_Identity=27.5109170305677, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6321091, Length=467, Percent_Identity=35.117773019272, Blast_Score=261, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6325240, Length=473, Percent_Identity=29.3868921775898, Blast_Score=192, Evalue=7e-50,
Organism=Saccharomyces cerevisiae, GI6325166, Length=460, Percent_Identity=27.8260869565217, Blast_Score=153, Evalue=5e-38,
Organism=Drosophila melanogaster, GI21358499, Length=469, Percent_Identity=39.0191897654584, Blast_Score=312, Evalue=3e-85,
Organism=Drosophila melanogaster, GI17737741, Length=469, Percent_Identity=22.3880597014925, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24640553, Length=467, Percent_Identity=22.6980728051392, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24640549, Length=467, Percent_Identity=22.6980728051392, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24640551, Length=467, Percent_Identity=22.6980728051392, Blast_Score=108, Evalue=8e-24,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50175; Mature: 50175

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEYDITVIGSGPGGYIAAIRAAQLGFKTAIVEKEENLGGICLNWGCIPTKSLLRASEVY
CCCEEEEEEECCCCCHHHHHHHHHHCHHEEEEECCCCCCCEEEECCCCCHHHHHHHHHHH
RLIRRSEEFGIKVKDASFNIQSMVKYSRNVVGKLSSGVEYLMKKNNIKVHQGFGKLAGNR
HHHHCCHHHCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHCCCC
TIKILNDKKEEEISSKHIILATGVRARNLPGIEVDGDLIWNAQHAMTPKKLPKSLLIIGS
EEEEECCCCHHHHCCCEEEEEECCCCCCCCCEEECCCEEECCHHHCCHHHCCCEEEEEEC
GAIGIEFASFYSTLGVDVTIIEVKDTILPLEDKDISNLAQEIFTKQRIKIYTNSSVKALT
CCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCEEEEEECCCEEEEC
KNKDFAQVLLSSGESKEFDRVIVAVGVQANTENIGLENTKIKLSSSGFIETNEWYETSES
CCCHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCEEEEECCCCEECCCCCCCCCC
SVYAIGDVAGPPCLAHKASHEAVICVEKIAGKNAHKLKKECIPNCTYSHPQVASVGLTEE
CEEEEECCCCCCHHHCCCCCCEEEEEHHHCCCCHHHHHHHHCCCCCCCCCCEEECCCCHH
QAIKSGYDIKVGKFHSNFNGKSIALSETEGLVKTIIDKKTGELLGSHMIGAEVTELISNF
HHHHCCCCEEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAKQLEGTDFDIKSTIFPHPTISEMIHESVLAADGESLNS
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNEYDITVIGSGPGGYIAAIRAAQLGFKTAIVEKEENLGGICLNWGCIPTKSLLRASEVY
CCCEEEEEEECCCCCHHHHHHHHHHCHHEEEEECCCCCCCEEEECCCCCHHHHHHHHHHH
RLIRRSEEFGIKVKDASFNIQSMVKYSRNVVGKLSSGVEYLMKKNNIKVHQGFGKLAGNR
HHHHCCHHHCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHCCCC
TIKILNDKKEEEISSKHIILATGVRARNLPGIEVDGDLIWNAQHAMTPKKLPKSLLIIGS
EEEEECCCCHHHHCCCEEEEEECCCCCCCCCEEECCCEEECCHHHCCHHHCCCEEEEEEC
GAIGIEFASFYSTLGVDVTIIEVKDTILPLEDKDISNLAQEIFTKQRIKIYTNSSVKALT
CCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCEEEEEECCCEEEEC
KNKDFAQVLLSSGESKEFDRVIVAVGVQANTENIGLENTKIKLSSSGFIETNEWYETSES
CCCHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCEEEEECCCCEECCCCCCCCCC
SVYAIGDVAGPPCLAHKASHEAVICVEKIAGKNAHKLKKECIPNCTYSHPQVASVGLTEE
CEEEEECCCCCCHHHCCCCCCEEEEEHHHCCCCHHHHHHHHCCCCCCCCCCEEECCCCHH
QAIKSGYDIKVGKFHSNFNGKSIALSETEGLVKTIIDKKTGELLGSHMIGAEVTELISNF
HHHHCCCCEEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAKQLEGTDFDIKSTIFPHPTISEMIHESVLAADGESLNS
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]