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The map label for this gene is mutM

Identifier: 190571281

GI number: 190571281

Start: 949120

End: 949935

Strand: Reverse

Name: mutM

Synonym: WPa_0882

Alternate gene names: 190571281

Gene position: 949935-949120 (Counterclockwise)

Preceding gene: 190571282

Following gene: 190571280

Centisome position: 64.08

GC content: 32.97

Gene sequence:

>816_bases
ATGCCAGAACTCCCAGAAGTGGAAGTCATTTCTAACTTCTTATTTGATAAAATCAAAAATAAGAAAATAAGCAATGTTAC
AGTCAATAATTGGAATTTGCGTGTACCAATAACAAAAAATATTGATGATTTGCTAAAGGGCAAAGTTATAAATGATATCA
AGCGTAGAGGTAAATATATAATCTCGAATATAGATGCTAGTATGGCTGTAATCATACACCTTGGCATGAGCGGAAAGCTT
ATATATGTTGAAGACAATCAAGCACAGAACAAACATGATCATGTGATATTCTTATTTTCTGACAACACTTCACTAATCTT
TAATGACCCAAGAAGGTTTGGATTAGTGATTGTTTTAAACAGAGAGCAAGAACTAAATTTTTTTAATAACCTAGGAATAG
AACCCCTCACAGATGAATTTGACGGACATTATTTACAGAAGTTGCTAAAGAACAGAAAAGCAAATATCAAATCAGTATTA
ATGAATAATAAGCTAATAGTTGGCGTAGGTAACATATATGCTTCTGAGAGCTTATTTAGAGCTCGCATATCACCACTTAG
GCTAGCACAAGATTTAACATATATAGAGTGCGAAAAACTTGCTATCGAAATAAAAAATACTCTGAGTGATGCAATTGCTG
CTGGTGGTTCAACACTGAAAGATTATGCACAACCATCTGGATCTGCTGGATACTTTCAAAATAACTTTTACGTATATGGT
AAAGTGCAAAAACCTTGCAGAATCTGCAACAATATCATAACACTTATACGACAAAATGGTCGTAGCACTTATTTTTGCAA
TGCATGTCAGAATTAA

Upstream 100 bases:

>100_bases
ATGAAAAAAAATAAGGAAAAAGAAGAAGAGAGAAAAAGATTGGCAAAAGCTTTAAAGCAAAATATTCTAAAAAGAAAAAA
ACAGCAACAGAGTAGACAAG

Downstream 100 bases:

>100_bases
ATTTTATTTTTGCATATGACACTCTTACCTGAAAAAGATCCTTTTGATTTGTTCTCAAAGTGGTATCAAGAAGTACTTAA
TTTTCCGTGTAAAGAGTCAA

Product: formamidopyrimidine-DNA glycosylase

Products: NA

Alternate protein names: Fapy-DNA glycosylase; DNA-(apurinic or apyrimidinic site) lyase mutM; AP lyase mutM

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MPELPEVEVISNFLFDKIKNKKISNVTVNNWNLRVPITKNIDDLLKGKVINDIKRRGKYIISNIDASMAVIIHLGMSGKL
IYVEDNQAQNKHDHVIFLFSDNTSLIFNDPRRFGLVIVLNREQELNFFNNLGIEPLTDEFDGHYLQKLLKNRKANIKSVL
MNNKLIVGVGNIYASESLFRARISPLRLAQDLTYIECEKLAIEIKNTLSDAIAAGGSTLKDYAQPSGSAGYFQNNFYVYG
KVQKPCRICNNIITLIRQNGRSTYFCNACQN

Sequences:

>Translated_271_residues
MPELPEVEVISNFLFDKIKNKKISNVTVNNWNLRVPITKNIDDLLKGKVINDIKRRGKYIISNIDASMAVIIHLGMSGKL
IYVEDNQAQNKHDHVIFLFSDNTSLIFNDPRRFGLVIVLNREQELNFFNNLGIEPLTDEFDGHYLQKLLKNRKANIKSVL
MNNKLIVGVGNIYASESLFRARISPLRLAQDLTYIECEKLAIEIKNTLSDAIAAGGSTLKDYAQPSGSAGYFQNNFYVYG
KVQKPCRICNNIITLIRQNGRSTYFCNACQN
>Mature_270_residues
PELPEVEVISNFLFDKIKNKKISNVTVNNWNLRVPITKNIDDLLKGKVINDIKRRGKYIISNIDASMAVIIHLGMSGKLI
YVEDNQAQNKHDHVIFLFSDNTSLIFNDPRRFGLVIVLNREQELNFFNNLGIEPLTDEFDGHYLQKLLKNRKANIKSVLM
NNKLIVGVGNIYASESLFRARISPLRLAQDLTYIECEKLAIEIKNTLSDAIAAGGSTLKDYAQPSGSAGYFQNNFYVYGK
VQKPCRICNNIITLIRQNGRSTYFCNACQN

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyr

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger

Homologues:

Organism=Escherichia coli, GI1790066, Length=271, Percent_Identity=42.4354243542435, Blast_Score=225, Evalue=3e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FPG_WOLPP (B3CM71)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975639.1
- EnsemblBacteria:   EBWOLT00000000932
- GeneID:   6384589
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0882
- GeneTree:   EBGT00050000030842
- HOGENOM:   HBG690070
- OMA:   RMTGQLL
- ProtClustDB:   CLSK749253
- HAMAP:   MF_00103
- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR000191
- InterPro:   IPR012319
- InterPro:   IPR020629
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663
- SMART:   SM00898
- TIGRFAMs:   TIGR00577

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS; SSF81624 Form_DNAglyc_cat; SSF46946 Ribosomal_H2TH

EC number: =3.2.2.23; =4.2.99.18

Molecular weight: Translated: 30780; Mature: 30649

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: PS51068 FPG_CAT; PS01242 ZF_FPG_1; PS51066 ZF_FPG_2

Important sites: ACT_SITE 2-2 ACT_SITE 3-3 ACT_SITE 58-58 ACT_SITE 261-261 BINDING 92-92 BINDING 111-111 BINDING 152-152

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELPEVEVISNFLFDKIKNKKISNVTVNNWNLRVPITKNIDDLLKGKVINDIKRRGKYI
CCCCCHHHHHHHHHHHHHCCCEECEEEEECCEEEEEECCCHHHHHHHHHHHHHHHCCCEE
ISNIDASMAVIIHLGMSGKLIYVEDNQAQNKHDHVIFLFSDNTSLIFNDPRRFGLVIVLN
EECCCCCEEEEEEECCCCEEEEEECCCCCCCCCEEEEEEECCCEEEEECCCCEEEEEEEE
REQELNFFNNLGIEPLTDEFDGHYLQKLLKNRKANIKSVLMNNKLIVGVGNIYASESLFR
CCHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHCCEEEEEECCHHHHHHHHH
ARISPLRLAQDLTYIECEKLAIEIKNTLSDAIAAGGSTLKDYAQPSGSAGYFQNNFYVYG
HHCCHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCEEECCEEEEE
KVQKPCRICNNIITLIRQNGRSTYFCNACQN
ECCCHHHHHHHHHHHHHCCCCCEEEEECCCC
>Mature Secondary Structure 
PELPEVEVISNFLFDKIKNKKISNVTVNNWNLRVPITKNIDDLLKGKVINDIKRRGKYI
CCCCHHHHHHHHHHHHHCCCEECEEEEECCEEEEEECCCHHHHHHHHHHHHHHHCCCEE
ISNIDASMAVIIHLGMSGKLIYVEDNQAQNKHDHVIFLFSDNTSLIFNDPRRFGLVIVLN
EECCCCCEEEEEEECCCCEEEEEECCCCCCCCCEEEEEEECCCEEEEECCCCEEEEEEEE
REQELNFFNNLGIEPLTDEFDGHYLQKLLKNRKANIKSVLMNNKLIVGVGNIYASESLFR
CCHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHCCEEEEEECCHHHHHHHHH
ARISPLRLAQDLTYIECEKLAIEIKNTLSDAIAAGGSTLKDYAQPSGSAGYFQNNFYVYG
HHCCHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCEEECCEEEEE
KVQKPCRICNNIITLIRQNGRSTYFCNACQN
ECCCHHHHHHHHHHHHHCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA