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The map label for this gene is mdh

Identifier: 190571254

GI number: 190571254

Start: 928884

End: 929828

Strand: Direct

Name: mdh

Synonym: WPa_0855

Alternate gene names: 190571254

Gene position: 928884-929828 (Clockwise)

Preceding gene: 190571253

Following gene: 190571258

Centisome position: 62.66

GC content: 36.93

Gene sequence:

>945_bases
ATGACAGCACAAAGAAAAAAAATATCTTTGATTGGTGCAGGAAATATCGGTGGGACTCTTGCCCATATGATTGCACTAAG
AGAGCTAGGAGATGTTATTTTGCTTGATGTAAGCGATGGAATACCACAAGGTAAAGCACTTGACATTACAGAATCATCTC
CTATTGATCGATCTAATGTCAATATTACTGGTACAAATAGGTATGAAGATATAAAAAACTCTGACGCAATTATAATAACT
GCTGGCATTGCCAGAAAACCTGGAATGAGCAGAGATGACCTTCTGCAAACTAATGCTGCAGTAATGAAAGAGGTTGGGGA
AAATATTAAAAAACATTCTCCAAACGCATTTGTAATAGTGGTTACCAATCCTTTAGATGCTATGGTTTCAGTAGTATACA
AATCTTCAAGCCTGCCAACTAATATGATTGTTGGTATGGCAGGAGTGCTTGACTCTGCTCGTTTTCGTTATTTTCTTGCA
AGTGAGTTAAATATTTCTGTCGAAGATGTATCTGCTTTTGTGCTTGGAGGACATGGTGACACTATGGTACCACTAATCAA
TTATGCTTCAATTGCTGGCATTCCACTTACCCAAATCATTGAGATGGGTGTGATTACGCGAGGAAAAGTTGATGAAATAG
TTGAGCGCACTCGTAATGGTGGAAAAGAAATAGTTGATCTACTTAAATCTGGATCTGCATACTATGCTCCTGCATCTTCA
GCCATAAGCATGCTAGAATCATACTTAAAAGATAAAAGGCGTATATTACCATGCGCTGCTTATCTTAATGGTGAATATGG
TGTGAAAGACTTATTCATTGGTGTTCCTACTATTATTGGCAAAAATGGAGTTGAGAAAGTTCTAGAAGTTAAAATGAACG
ATAGTGAACAAGAGATGTTTTATAAATCTGTAAGTGCAGTAAAAGAGCTCTGTCAGCTAAATTAA

Upstream 100 bases:

>100_bases
ATATAATCCTATAGACTTACCACAAGATTTTCGTATGTTTGACAGCTTATCCCCTTGGGAAGTTAGTAAAGCCACAAAAG
TAAATATAAAGGAGTAGAAA

Downstream 100 bases:

>100_bases
TTGCCCTATAGAATTTCTTTGAGCTCAAGCTTCGAAAACAGTCTAAAAAAATTATTTGTAGTTATTTTTGCTACTTCTTC
TGGCGATTCATTCCATAATT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MTAQRKKISLIGAGNIGGTLAHMIALRELGDVILLDVSDGIPQGKALDITESSPIDRSNVNITGTNRYEDIKNSDAIIIT
AGIARKPGMSRDDLLQTNAAVMKEVGENIKKHSPNAFVIVVTNPLDAMVSVVYKSSSLPTNMIVGMAGVLDSARFRYFLA
SELNISVEDVSAFVLGGHGDTMVPLINYASIAGIPLTQIIEMGVITRGKVDEIVERTRNGGKEIVDLLKSGSAYYAPASS
AISMLESYLKDKRRILPCAAYLNGEYGVKDLFIGVPTIIGKNGVEKVLEVKMNDSEQEMFYKSVSAVKELCQLN

Sequences:

>Translated_314_residues
MTAQRKKISLIGAGNIGGTLAHMIALRELGDVILLDVSDGIPQGKALDITESSPIDRSNVNITGTNRYEDIKNSDAIIIT
AGIARKPGMSRDDLLQTNAAVMKEVGENIKKHSPNAFVIVVTNPLDAMVSVVYKSSSLPTNMIVGMAGVLDSARFRYFLA
SELNISVEDVSAFVLGGHGDTMVPLINYASIAGIPLTQIIEMGVITRGKVDEIVERTRNGGKEIVDLLKSGSAYYAPASS
AISMLESYLKDKRRILPCAAYLNGEYGVKDLFIGVPTIIGKNGVEKVLEVKMNDSEQEMFYKSVSAVKELCQLN
>Mature_313_residues
TAQRKKISLIGAGNIGGTLAHMIALRELGDVILLDVSDGIPQGKALDITESSPIDRSNVNITGTNRYEDIKNSDAIIITA
GIARKPGMSRDDLLQTNAAVMKEVGENIKKHSPNAFVIVVTNPLDAMVSVVYKSSSLPTNMIVGMAGVLDSARFRYFLAS
ELNISVEDVSAFVLGGHGDTMVPLINYASIAGIPLTQIIEMGVITRGKVDEIVERTRNGGKEIVDLLKSGSAYYAPASSA
ISMLESYLKDKRRILPCAAYLNGEYGVKDLFIGVPTIIGKNGVEKVLEVKMNDSEQEMFYKSVSAVKELCQLN

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family

Homologues:

Organism=Homo sapiens, GI47059044, Length=315, Percent_Identity=34.2857142857143, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI221136809, Length=315, Percent_Identity=34.2857142857143, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI15082234, Length=315, Percent_Identity=32.3809523809524, Blast_Score=174, Evalue=7e-44,
Organism=Homo sapiens, GI9257228, Length=305, Percent_Identity=32.4590163934426, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI4504973, Length=305, Percent_Identity=32.4590163934426, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI5031857, Length=303, Percent_Identity=31.6831683168317, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI260099723, Length=303, Percent_Identity=31.6831683168317, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI291575128, Length=313, Percent_Identity=30.6709265175719, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI4557032, Length=313, Percent_Identity=30.6709265175719, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI260099725, Length=196, Percent_Identity=34.1836734693878, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI260099727, Length=196, Percent_Identity=34.1836734693878, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI207028494, Length=187, Percent_Identity=30.4812834224599, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI21735621, Length=250, Percent_Identity=27.6, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI103472011, Length=315, Percent_Identity=22.8571428571429, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1789632, Length=293, Percent_Identity=29.0102389078498, Blast_Score=100, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17535107, Length=284, Percent_Identity=25.7042253521127, Blast_Score=120, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17554310, Length=318, Percent_Identity=29.874213836478, Blast_Score=101, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6322765, Length=304, Percent_Identity=26.9736842105263, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320125, Length=213, Percent_Identity=26.2910798122066, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324446, Length=254, Percent_Identity=28.3464566929134, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17136226, Length=303, Percent_Identity=28.7128712871287, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI45550422, Length=317, Percent_Identity=28.0757097791798, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24647881, Length=320, Percent_Identity=28.125, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24663595, Length=281, Percent_Identity=26.3345195729537, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24663599, Length=247, Percent_Identity=27.9352226720648, Blast_Score=84, Evalue=8e-17,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_WOLPP (B3CM44)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975612.1
- ProteinModelPortal:   B3CM44
- EnsemblBacteria:   EBWOLT00000000425
- GeneID:   6385042
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0855
- GeneTree:   EBGT00050000031315
- HOGENOM:   HBG566126
- OMA:   YAVYKES
- ProtClustDB:   PRK06223
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00487
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01763

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 33828; Mature: 33696

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: ACT_SITE 178-178 BINDING 36-36 BINDING 85-85 BINDING 91-91 BINDING 98-98 BINDING 123-123 BINDING 154-154

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQRKKISLIGAGNIGGTLAHMIALRELGDVILLDVSDGIPQGKALDITESSPIDRSNV
CCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCEEECCCCCCCCCCCE
NITGTNRYEDIKNSDAIIITAGIARKPGMSRDDLLQTNAAVMKEVGENIKKHSPNAFVIV
EEEECCCHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
VTNPLDAMVSVVYKSSSLPTNMIVGMAGVLDSARFRYFLASELNISVEDVSAFVLGGHGD
EECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCEEHHHEEEEEECCCCC
TMVPLINYASIAGIPLTQIIEMGVITRGKVDEIVERTRNGGKEIVDLLKSGSAYYAPASS
CHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCEECCHHH
AISMLESYLKDKRRILPCAAYLNGEYGVKDLFIGVPTIIGKNGVEKVLEVKMNDSEQEMF
HHHHHHHHHHHHHHEEHHHEEECCCCCHHHHHCCCCHHHCCHHHHHHHHHHCCCCHHHHH
YKSVSAVKELCQLN
HHHHHHHHHHHCCC
>Mature Secondary Structure 
TAQRKKISLIGAGNIGGTLAHMIALRELGDVILLDVSDGIPQGKALDITESSPIDRSNV
CCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCEEECCCCCCCCCCCE
NITGTNRYEDIKNSDAIIITAGIARKPGMSRDDLLQTNAAVMKEVGENIKKHSPNAFVIV
EEEECCCHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
VTNPLDAMVSVVYKSSSLPTNMIVGMAGVLDSARFRYFLASELNISVEDVSAFVLGGHGD
EECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCEEHHHEEEEEECCCCC
TMVPLINYASIAGIPLTQIIEMGVITRGKVDEIVERTRNGGKEIVDLLKSGSAYYAPASS
CHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCEECCHHH
AISMLESYLKDKRRILPCAAYLNGEYGVKDLFIGVPTIIGKNGVEKVLEVKMNDSEQEMF
HHHHHHHHHHHHHHEEHHHEEECCCCCHHHHHCCCCHHHCCHHHHHHHHHHCCCCHHHHH
YKSVSAVKELCQLN
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA