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The map label for this gene is glyA

Identifier: 190571236

GI number: 190571236

Start: 914173

End: 915450

Strand: Reverse

Name: glyA

Synonym: WPa_0836

Alternate gene names: 190571236

Gene position: 915450-914173 (Counterclockwise)

Preceding gene: 190571237

Following gene: 190571235

Centisome position: 61.75

GC content: 39.2

Gene sequence:

>1278_bases
ATGACAATTGCTTCAGAAAGGATTTGTAACTCTGAAAATAATTTAAAGTCTTGTGATAATGAAGTTTATCTCTCTATAGA
GAAGGAACTGCAGCGTCAAAGATCGCAGTTGCAATTGATTGCATCGGAGAATTTTGCAAGTAAAGCAGTGATGGAAGCAC
AGGGTTCTTTTCTGACTAATAAATATGCAGAAGGTTATCCTGGTAAAAGATATTACTGTGGCTGTGAGTATGTAGATGAA
GTTGAAAATCTGGCTATAGAAAGGCTTTGTAAGCTGTTTAATGTTAAATTTGCAAATGTTCAACCTCACTCTGGTTCTCA
GGCAAATCAAGCAGTGTTTGCTTCACTACTTACTCCGGGTGATACAATACTTGGATTGTCACTGAATTGTGGTGGACATT
TAACTCATGGTGCAGCACCAAACCTTTCTGGTAAGTGGTTTAAGTCGATCCAGTATACAGTTAATAGAGACACTTACCTG
CTTGATATGGATGAGGTGGAAAGGCTAGCGCTAGAGCATAAACCGAAGCTAATCATTGCTGGTGCTTCTGCTTATCCAAG
AAAAATAGATTTCGAGCGCTTTCGTGAAATTGCGAATAAAGTTGGTGCATATCTGCTTGCAGATATTGCTCACTATTCAG
GGCTTATTGCTGCAGGTTGTTACCCATCACCTGCTGAATATGCGCATATTATAACTTCTACAACTCACAAGACTCTACGT
GGTCCTCGTGGTGGAGTGGTAATGACAAATGATGAAGCGTTACATAAAAAAATTCAGTCTGCAGTTTTTCCAGGATTGCA
AGGTGGGCCACTTATGCATGTGATAGCTGCAAAAGCTGTTGCATTTAAGGAAGCATTAGCGCCAGAGTTTAAAACTTATA
GTAAAAAAGTTGTGGAAAATGCGAAAGTGTTAGCTCAAGCGCTGCAAGGGCATGGGCTGAACATTATAACCGGTGGCACT
GACTCTCATATAGTGCTAGTTGATTTAAGATCACAGAAGTTGAAGGGAAAAGACGTTGTAAATAGCCTTGAGAGAGCTGG
CATTACCTGCAATAAAAACTCCGTGCCGTTTGACACAGAAAAACCAACCATTACTTCAGGGCTCCGTTTTGGTACCGCTG
CTGAGACAACACGCGGACTTGAGAAAAAAGATTTTAAAGAGATAGCTGATCTGATAAATGAAATAATTCAGGGATTAATT
GATGGAAATAGCCCTGATGTTGAAAAAGCAGTGAAAAATAAAGTTGAAAGTATTTGTAGTAATTTTCCTATTTATTAA

Upstream 100 bases:

>100_bases
CCTGTGTATGGTACTGAGTTACTAGTAAAAGCAATATCAATTATTCCTGTATGTTTGCTTTTGGCTGTTTTTGGATTTAT
GTGGTTAAGGAAAAGGTCTT

Downstream 100 bases:

>100_bases
TTTATTTAGCATTGGAATTTCCTTCCTTTACTTTGGATTAAACTATTGTATAGTGATTTTATACCTCTTTAATTTTATGA
TAAATGTACCTAGCATAGAG

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MTIASERICNSENNLKSCDNEVYLSIEKELQRQRSQLQLIASENFASKAVMEAQGSFLTNKYAEGYPGKRYYCGCEYVDE
VENLAIERLCKLFNVKFANVQPHSGSQANQAVFASLLTPGDTILGLSLNCGGHLTHGAAPNLSGKWFKSIQYTVNRDTYL
LDMDEVERLALEHKPKLIIAGASAYPRKIDFERFREIANKVGAYLLADIAHYSGLIAAGCYPSPAEYAHIITSTTHKTLR
GPRGGVVMTNDEALHKKIQSAVFPGLQGGPLMHVIAAKAVAFKEALAPEFKTYSKKVVENAKVLAQALQGHGLNIITGGT
DSHIVLVDLRSQKLKGKDVVNSLERAGITCNKNSVPFDTEKPTITSGLRFGTAAETTRGLEKKDFKEIADLINEIIQGLI
DGNSPDVEKAVKNKVESICSNFPIY

Sequences:

>Translated_425_residues
MTIASERICNSENNLKSCDNEVYLSIEKELQRQRSQLQLIASENFASKAVMEAQGSFLTNKYAEGYPGKRYYCGCEYVDE
VENLAIERLCKLFNVKFANVQPHSGSQANQAVFASLLTPGDTILGLSLNCGGHLTHGAAPNLSGKWFKSIQYTVNRDTYL
LDMDEVERLALEHKPKLIIAGASAYPRKIDFERFREIANKVGAYLLADIAHYSGLIAAGCYPSPAEYAHIITSTTHKTLR
GPRGGVVMTNDEALHKKIQSAVFPGLQGGPLMHVIAAKAVAFKEALAPEFKTYSKKVVENAKVLAQALQGHGLNIITGGT
DSHIVLVDLRSQKLKGKDVVNSLERAGITCNKNSVPFDTEKPTITSGLRFGTAAETTRGLEKKDFKEIADLINEIIQGLI
DGNSPDVEKAVKNKVESICSNFPIY
>Mature_424_residues
TIASERICNSENNLKSCDNEVYLSIEKELQRQRSQLQLIASENFASKAVMEAQGSFLTNKYAEGYPGKRYYCGCEYVDEV
ENLAIERLCKLFNVKFANVQPHSGSQANQAVFASLLTPGDTILGLSLNCGGHLTHGAAPNLSGKWFKSIQYTVNRDTYLL
DMDEVERLALEHKPKLIIAGASAYPRKIDFERFREIANKVGAYLLADIAHYSGLIAAGCYPSPAEYAHIITSTTHKTLRG
PRGGVVMTNDEALHKKIQSAVFPGLQGGPLMHVIAAKAVAFKEALAPEFKTYSKKVVENAKVLAQALQGHGLNIITGGTD
SHIVLVDLRSQKLKGKDVVNSLERAGITCNKNSVPFDTEKPTITSGLRFGTAAETTRGLEKKDFKEIADLINEIIQGLID
GNSPDVEKAVKNKVESICSNFPIY

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI19923315, Length=413, Percent_Identity=45.7627118644068, Blast_Score=344, Evalue=1e-94,
Organism=Homo sapiens, GI261862352, Length=413, Percent_Identity=45.7627118644068, Blast_Score=343, Evalue=1e-94,
Organism=Homo sapiens, GI261862350, Length=413, Percent_Identity=45.7627118644068, Blast_Score=343, Evalue=1e-94,
Organism=Homo sapiens, GI261862348, Length=413, Percent_Identity=45.7627118644068, Blast_Score=343, Evalue=1e-94,
Organism=Homo sapiens, GI22547186, Length=408, Percent_Identity=48.7745098039216, Blast_Score=338, Evalue=5e-93,
Organism=Homo sapiens, GI261862346, Length=413, Percent_Identity=45.2784503631961, Blast_Score=331, Evalue=7e-91,
Organism=Homo sapiens, GI22547189, Length=394, Percent_Identity=46.1928934010152, Blast_Score=304, Evalue=8e-83,
Organism=Escherichia coli, GI1788902, Length=420, Percent_Identity=51.9047619047619, Blast_Score=429, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI25144729, Length=423, Percent_Identity=45.8628841607565, Blast_Score=361, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI25144732, Length=423, Percent_Identity=45.8628841607565, Blast_Score=360, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6319739, Length=402, Percent_Identity=43.5323383084577, Blast_Score=337, Evalue=3e-93,
Organism=Saccharomyces cerevisiae, GI6323087, Length=408, Percent_Identity=44.8529411764706, Blast_Score=336, Evalue=4e-93,
Organism=Drosophila melanogaster, GI24640005, Length=408, Percent_Identity=47.0588235294118, Blast_Score=357, Evalue=1e-98,
Organism=Drosophila melanogaster, GI221329721, Length=408, Percent_Identity=47.0588235294118, Blast_Score=356, Evalue=2e-98,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_WOLPP (B3CM26)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975594.1
- ProteinModelPortal:   B3CM26
- EnsemblBacteria:   EBWOLT00000000845
- GeneID:   6384595
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0836
- GeneTree:   EBGT00050000031396
- HOGENOM:   HBG301263
- OMA:   RAVFPGY
- ProtClustDB:   PRK00011
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 46514; Mature: 46382

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIASERICNSENNLKSCDNEVYLSIEKELQRQRSQLQLIASENFASKAVMEAQGSFLTN
CCCCHHHHCCCCCCHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHH
KYAEGYPGKRYYCGCEYVDEVENLAIERLCKLFNVKFANVQPHSGSQANQAVFASLLTPG
HHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHHHCCCC
DTILGLSLNCGGHLTHGAAPNLSGKWFKSIQYTVNRDTYLLDMDEVERLALEHKPKLIIA
CEEEEEEECCCCCCCCCCCCCCCCHHHHHEEEEECCCEEEEEHHHHHHHHHHCCCCEEEE
GASAYPRKIDFERFREIANKVGAYLLADIAHYSGLIAAGCYPSPAEYAHIITSTTHKTLR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHC
GPRGGVVMTNDEALHKKIQSAVFPGLQGGPLMHVIAAKAVAFKEALAPEFKTYSKKVVEN
CCCCCEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
AKVLAQALQGHGLNIITGGTDSHIVLVDLRSQKLKGKDVVNSLERAGITCNKNSVPFDTE
HHHHHHHHHCCCCEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHCCCEECCCCCCCCCC
KPTITSGLRFGTAAETTRGLEKKDFKEIADLINEIIQGLIDGNSPDVEKAVKNKVESICS
CCCHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
NFPIY
CCCCC
>Mature Secondary Structure 
TIASERICNSENNLKSCDNEVYLSIEKELQRQRSQLQLIASENFASKAVMEAQGSFLTN
CCCHHHHCCCCCCHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHH
KYAEGYPGKRYYCGCEYVDEVENLAIERLCKLFNVKFANVQPHSGSQANQAVFASLLTPG
HHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHHHCCCC
DTILGLSLNCGGHLTHGAAPNLSGKWFKSIQYTVNRDTYLLDMDEVERLALEHKPKLIIA
CEEEEEEECCCCCCCCCCCCCCCCHHHHHEEEEECCCEEEEEHHHHHHHHHHCCCCEEEE
GASAYPRKIDFERFREIANKVGAYLLADIAHYSGLIAAGCYPSPAEYAHIITSTTHKTLR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHC
GPRGGVVMTNDEALHKKIQSAVFPGLQGGPLMHVIAAKAVAFKEALAPEFKTYSKKVVEN
CCCCCEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
AKVLAQALQGHGLNIITGGTDSHIVLVDLRSQKLKGKDVVNSLERAGITCNKNSVPFDTE
HHHHHHHHHCCCCEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHCCCEECCCCCCCCCC
KPTITSGLRFGTAAETTRGLEKKDFKEIADLINEIIQGLIDGNSPDVEKAVKNKVESICS
CCCHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
NFPIY
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA