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The map label for this gene is pgk

Identifier: 190571235

GI number: 190571235

Start: 912906

End: 914096

Strand: Reverse

Name: pgk

Synonym: WPa_0835

Alternate gene names: 190571235

Gene position: 914096-912906 (Counterclockwise)

Preceding gene: 190571236

Following gene: 190571234

Centisome position: 61.66

GC content: 35.94

Gene sequence:

>1191_bases
ATGATAAATGTACCTAGCATAGAGAGTTGTGATTTTCACAATAAAAATGTTCTGCTCAGGGTTGACTTCAATGTTCCTAT
AAAAAATGGAAGAATTTGTGATGCCACTCGTATTTTGAGAGCGCTACCTACCATTCAGTATTTGGCAAATGCGGGTGCAA
AAGTTATTGTTATCTCACATTTTGGGCGTCCAAAAGCCAAAGACAGTAACTTATCGCTAAAAAACGTAGTTGAAACTTTA
TCGCGGCTGCTAAGCAAAGAAGTGAAATTTATTGATGATTGTATTGGTGAGAGAGTACAAAGAGCAATAAATGCGATGGA
TGGAGGAGATATAATATTACTAGAGAATCTGAGGTTTTATAAAGAAGAAGAGCAGAATGATTCAAATTTTGCTAAACAAT
TAGCATCTCTAGCAGATATATATATAAATGATGCGTTTTCTTGCTCTCACAGAGCTCACGCTTCTATTTCACGCATTACG
GAATTTTTACCTTCTTATGCAGGATTTTGCTTGCAAGATGAGCTAAAGTATCTTGAGCAAGCTATATCGTTTGACGCTAA
ACCTATTACTGCGATAGTTGGTGGAGCTAAAATATCAACTAAAATAAAAATGCTTATAAAGCTAGCAGAAAAGGTTGATT
ACCTTATTCTGGGCGGCGCAATTGCTAATAATTTTTTGTTATTTAATAAAGTTAATATAGGCAAGTCTTTTTTTCAAAAT
GGCGTTGATGATCTTCTGCACGATATTGTTGAGACGGCAAATAAAAACAATTGCAAAATAGTTGTGCCAGAAGACGTTTT
GGTTGCAGTAAATTCTGATTATAGCACTGGCGTTTTAAGAAAAATTGAATCCATTTTGGACGGTGATATAATTTTAGATA
TCGGACCTCAAACTTTAAGCACAATAAGCGGTATAATAGCAAGCAGTAAAACTCTGCTGTGGAACGGACCTATTGGTGTT
TTTGAACATTCAGCTTTTGCAAATGGTACAGTAGAGGTGATGAGAGTAGTAAGTGATTTGACACACGAAGGAAAATTAAC
CAGTGTAATAGGAGGAGGAGATAGTCTATCTGCAATAAATACTGCAGGTCTTGCCGATAAAGATTTTACATATATTTCAA
CTGGTGGGGGAGCGTTTTTAAGTTGGCTAAGTGGTGATGAGATGCCAGGACTGCGATCAACATTAGATTAA

Upstream 100 bases:

>100_bases
TGTAGTAATTTTCCTATTTATTAATTTATTTAGCATTGGAATTTCCTTCCTTTACTTTGGATTAAACTATTGTATAGTGA
TTTTATACCTCTTTAATTTT

Downstream 100 bases:

>100_bases
AACTTTTTTAAGTTGTTGCTTATACCCTAGCTTCTTTATGAATATGATTATGTCGAAAATAGCTAAGTTAATCTTGTGCT
TAATAATACCAGTAATAGGA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MINVPSIESCDFHNKNVLLRVDFNVPIKNGRICDATRILRALPTIQYLANAGAKVIVISHFGRPKAKDSNLSLKNVVETL
SRLLSKEVKFIDDCIGERVQRAINAMDGGDIILLENLRFYKEEEQNDSNFAKQLASLADIYINDAFSCSHRAHASISRIT
EFLPSYAGFCLQDELKYLEQAISFDAKPITAIVGGAKISTKIKMLIKLAEKVDYLILGGAIANNFLLFNKVNIGKSFFQN
GVDDLLHDIVETANKNNCKIVVPEDVLVAVNSDYSTGVLRKIESILDGDIILDIGPQTLSTISGIIASSKTLLWNGPIGV
FEHSAFANGTVEVMRVVSDLTHEGKLTSVIGGGDSLSAINTAGLADKDFTYISTGGGAFLSWLSGDEMPGLRSTLD

Sequences:

>Translated_396_residues
MINVPSIESCDFHNKNVLLRVDFNVPIKNGRICDATRILRALPTIQYLANAGAKVIVISHFGRPKAKDSNLSLKNVVETL
SRLLSKEVKFIDDCIGERVQRAINAMDGGDIILLENLRFYKEEEQNDSNFAKQLASLADIYINDAFSCSHRAHASISRIT
EFLPSYAGFCLQDELKYLEQAISFDAKPITAIVGGAKISTKIKMLIKLAEKVDYLILGGAIANNFLLFNKVNIGKSFFQN
GVDDLLHDIVETANKNNCKIVVPEDVLVAVNSDYSTGVLRKIESILDGDIILDIGPQTLSTISGIIASSKTLLWNGPIGV
FEHSAFANGTVEVMRVVSDLTHEGKLTSVIGGGDSLSAINTAGLADKDFTYISTGGGAFLSWLSGDEMPGLRSTLD
>Mature_396_residues
MINVPSIESCDFHNKNVLLRVDFNVPIKNGRICDATRILRALPTIQYLANAGAKVIVISHFGRPKAKDSNLSLKNVVETL
SRLLSKEVKFIDDCIGERVQRAINAMDGGDIILLENLRFYKEEEQNDSNFAKQLASLADIYINDAFSCSHRAHASISRIT
EFLPSYAGFCLQDELKYLEQAISFDAKPITAIVGGAKISTKIKMLIKLAEKVDYLILGGAIANNFLLFNKVNIGKSFFQN
GVDDLLHDIVETANKNNCKIVVPEDVLVAVNSDYSTGVLRKIESILDGDIILDIGPQTLSTISGIIASSKTLLWNGPIGV
FEHSAFANGTVEVMRVVSDLTHEGKLTSVIGGGDSLSAINTAGLADKDFTYISTGGGAFLSWLSGDEMPGLRSTLD

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=408, Percent_Identity=37.2549019607843, Blast_Score=260, Evalue=2e-69,
Organism=Homo sapiens, GI31543397, Length=410, Percent_Identity=37.0731707317073, Blast_Score=255, Evalue=5e-68,
Organism=Escherichia coli, GI1789294, Length=397, Percent_Identity=38.2871536523929, Blast_Score=257, Evalue=8e-70,
Organism=Caenorhabditis elegans, GI17508823, Length=410, Percent_Identity=38.780487804878, Blast_Score=276, Evalue=1e-74,
Organism=Saccharomyces cerevisiae, GI6319857, Length=406, Percent_Identity=37.9310344827586, Blast_Score=267, Evalue=2e-72,
Organism=Drosophila melanogaster, GI17136394, Length=411, Percent_Identity=39.9026763990268, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24581203, Length=412, Percent_Identity=37.621359223301, Blast_Score=258, Evalue=4e-69,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_WOLPP (B3CM25)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975593.1
- EnsemblBacteria:   EBWOLT00000000029
- GeneID:   6385565
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0835
- GeneTree:   EBGT00050000030985
- HOGENOM:   HBG453500
- OMA:   RITEFLP
- ProtClustDB:   PRK00073
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 43060; Mature: 43060

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 37-37 BINDING 118-118 BINDING 151-151 BINDING 201-201 BINDING 322-322

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINVPSIESCDFHNKNVLLRVDFNVPIKNGRICDATRILRALPTIQYLANAGAKVIVISH
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEE
FGRPKAKDSNLSLKNVVETLSRLLSKEVKFIDDCIGERVQRAINAMDGGDIILLENLRFY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHH
KEEEQNDSNFAKQLASLADIYINDAFSCSHRAHASISRITEFLPSYAGFCLQDELKYLEQ
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
AISFDAKPITAIVGGAKISTKIKMLIKLAEKVDYLILGGAIANNFLLFNKVNIGKSFFQN
HHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCEEEECHHHHCCEEEEEEECCCHHHHHC
GVDDLLHDIVETANKNNCKIVVPEDVLVAVNSDYSTGVLRKIESILDGDIILDIGPQTLS
CHHHHHHHHHHHCCCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCHHHH
TISGIIASSKTLLWNGPIGVFEHSAFANGTVEVMRVVSDLTHEGKLTSVIGGGDSLSAIN
HHHHHHHCCCEEEEECCCCHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHH
TAGLADKDFTYISTGGGAFLSWLSGDEMPGLRSTLD
HCCCCCCCCEEEECCCHHHHHHCCCCCCCCCHHCCC
>Mature Secondary Structure
MINVPSIESCDFHNKNVLLRVDFNVPIKNGRICDATRILRALPTIQYLANAGAKVIVISH
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEE
FGRPKAKDSNLSLKNVVETLSRLLSKEVKFIDDCIGERVQRAINAMDGGDIILLENLRFY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHH
KEEEQNDSNFAKQLASLADIYINDAFSCSHRAHASISRITEFLPSYAGFCLQDELKYLEQ
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
AISFDAKPITAIVGGAKISTKIKMLIKLAEKVDYLILGGAIANNFLLFNKVNIGKSFFQN
HHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCEEEECHHHHCCEEEEEEECCCHHHHHC
GVDDLLHDIVETANKNNCKIVVPEDVLVAVNSDYSTGVLRKIESILDGDIILDIGPQTLS
CHHHHHHHHHHHCCCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCHHHH
TISGIIASSKTLLWNGPIGVFEHSAFANGTVEVMRVVSDLTHEGKLTSVIGGGDSLSAIN
HHHHHHHCCCEEEEECCCCHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHH
TAGLADKDFTYISTGGGAFLSWLSGDEMPGLRSTLD
HCCCCCCCCEEEECCCHHHHHHCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA