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The map label for this gene is ampD [H]

Identifier: 190571233

GI number: 190571233

Start: 910479

End: 911954

Strand: Reverse

Name: ampD [H]

Synonym: WPa_0833

Alternate gene names: 190571233

Gene position: 911954-910479 (Counterclockwise)

Preceding gene: 190571234

Following gene: 190571232

Centisome position: 61.52

GC content: 31.64

Gene sequence:

>1476_bases
ATGAAACTTATAGTAAAAAAATGTTTGTCTGTTTTATTTCTGTTGCTGCTTGTAGCGCCTCTTTTATATGGACAACAATC
TTGTTTAAGTGATATTGAGAACGATTTTCAAGATTTGAAAACATCATTAAAAAATCCAATATTTTTAGATCCTGCACCGA
GTTTAAATTATGATGATAGAGAAGGCAAAAAAGTGTTGATGGTGGTGGTGCACCATACCGAATCACCAACATTAAAAAGT
ACAAAATACGCATTAAATTCTTCAGGGATATCAGTACAGCTTATTGTTGATAGAGATGGTAGCATTACTCTGATGGTTCC
ACTTGAAAAACGAGCATGGCATGCTGGTATAAGTTATGCGAAAGTTCAAGTAGATAATGTGGTTGAAGAGCTACGAAAAC
TGAATGATTATTCTATAGGTATAGAGATTGTAAACACAGGGCTTGAGCCTTTTCCAGAAGAGCAAATGAAATCTGTCAAA
GATCTGATTTTGTACCTCATGAAGCGCTTTAAGATTAGAAAAGATATGATATTTAGTCACGCTGAGATAGGCACTATAGT
GTACAACTCAGCAATTGAAGGTTACGCAATGCGTAAGCCTGATCCACATAAGTTATTTGATTGGGAGTTATTAGAGAAAA
GTGGTATTGGATTGCATATAGGTGATAGAATCAGTCCTCAAGACGCAGAACAAAAAGTAAATGAGGTTTTGTATAAGGTA
GGTGATAAGAGTGAAAATATTTTGAAATTAAAAAAAAGGTTAAACGGTTTTTTTTATAAAATACTGCCTTGGAATGATAG
GGAAGGTAATATGATTTTTCCTGACAATAATGCTAATTACTCAAATGAGTTTGATGAAAATTTTGCGTGGGTTATCAATC
AGTTTTCAATGCACCACTTGCCGAAAGAGATTAGAAAAGACTTGCCTCTAAAATTAGAACAAGAAGATATTTTGCCTGAA
TTTTTAGTTAAGTATAGAAATCTTATTTTTAGTGAATTCTTATCTTTAAGTGATAAAACTCAATTAAGCTTAAAGCCACC
ATTTTTGAATGAAGAGGATTATAAGCATTTATTATCTTCTCTTGCAGAATATGAAAATAATATTTCTTCTGATGCATTTG
CTATGCTCATGTACAAGATTAAGCTATATTATGACTCTTATCTAAGATACAATATAAGATCTTCACTTTATATTCCATTT
AGACTTAATTCATTTGAAAAGCTAGATATTTTAAAGAATGAAATATTATCTTTTAAGTCTATAAGCCCTGAAAAGGCTAT
AGAAGTCTCGAATTTGATTAATGAGTTTAGGTCGAAAATTTTATCTGATTTCCAAAATTTTGAAAAGCAATGGTTTCAAG
AATTTAAGGACACTTGGAAACAAAAATTTATACCGAATATGGAAAGGCAAATGTCTTGGACTGCGCTACATGAAACTGTG
TTGGAATATTTAGAAAAGGCGAAGAAAGAAGTCTAA

Upstream 100 bases:

>100_bases
TTAATTGTAGCTGTAGCAAGTCTTGATTGGCTGAAAAAAAGTAAACATTTTATGTGTGTTATGCTAATTTAGTTATATTA
AGGGCAATTTGTAGAGCTAC

Downstream 100 bases:

>100_bases
TGAGTATTACAATGACCAGATGCATGGGCAGAGAAAAATAAAGGCACATGCATCTGGAAGTATACAAAAATTTCTAAATG
AGATTAATATAGTATTGGGA

Product: N-acetylmuramoyl-L-alanine amidase, family 2

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MKLIVKKCLSVLFLLLLVAPLLYGQQSCLSDIENDFQDLKTSLKNPIFLDPAPSLNYDDREGKKVLMVVVHHTESPTLKS
TKYALNSSGISVQLIVDRDGSITLMVPLEKRAWHAGISYAKVQVDNVVEELRKLNDYSIGIEIVNTGLEPFPEEQMKSVK
DLILYLMKRFKIRKDMIFSHAEIGTIVYNSAIEGYAMRKPDPHKLFDWELLEKSGIGLHIGDRISPQDAEQKVNEVLYKV
GDKSENILKLKKRLNGFFYKILPWNDREGNMIFPDNNANYSNEFDENFAWVINQFSMHHLPKEIRKDLPLKLEQEDILPE
FLVKYRNLIFSEFLSLSDKTQLSLKPPFLNEEDYKHLLSSLAEYENNISSDAFAMLMYKIKLYYDSYLRYNIRSSLYIPF
RLNSFEKLDILKNEILSFKSISPEKAIEVSNLINEFRSKILSDFQNFEKQWFQEFKDTWKQKFIPNMERQMSWTALHETV
LEYLEKAKKEV

Sequences:

>Translated_491_residues
MKLIVKKCLSVLFLLLLVAPLLYGQQSCLSDIENDFQDLKTSLKNPIFLDPAPSLNYDDREGKKVLMVVVHHTESPTLKS
TKYALNSSGISVQLIVDRDGSITLMVPLEKRAWHAGISYAKVQVDNVVEELRKLNDYSIGIEIVNTGLEPFPEEQMKSVK
DLILYLMKRFKIRKDMIFSHAEIGTIVYNSAIEGYAMRKPDPHKLFDWELLEKSGIGLHIGDRISPQDAEQKVNEVLYKV
GDKSENILKLKKRLNGFFYKILPWNDREGNMIFPDNNANYSNEFDENFAWVINQFSMHHLPKEIRKDLPLKLEQEDILPE
FLVKYRNLIFSEFLSLSDKTQLSLKPPFLNEEDYKHLLSSLAEYENNISSDAFAMLMYKIKLYYDSYLRYNIRSSLYIPF
RLNSFEKLDILKNEILSFKSISPEKAIEVSNLINEFRSKILSDFQNFEKQWFQEFKDTWKQKFIPNMERQMSWTALHETV
LEYLEKAKKEV
>Mature_491_residues
MKLIVKKCLSVLFLLLLVAPLLYGQQSCLSDIENDFQDLKTSLKNPIFLDPAPSLNYDDREGKKVLMVVVHHTESPTLKS
TKYALNSSGISVQLIVDRDGSITLMVPLEKRAWHAGISYAKVQVDNVVEELRKLNDYSIGIEIVNTGLEPFPEEQMKSVK
DLILYLMKRFKIRKDMIFSHAEIGTIVYNSAIEGYAMRKPDPHKLFDWELLEKSGIGLHIGDRISPQDAEQKVNEVLYKV
GDKSENILKLKKRLNGFFYKILPWNDREGNMIFPDNNANYSNEFDENFAWVINQFSMHHLPKEIRKDLPLKLEQEDILPE
FLVKYRNLIFSEFLSLSDKTQLSLKPPFLNEEDYKHLLSSLAEYENNISSDAFAMLMYKIKLYYDSYLRYNIRSSLYIPF
RLNSFEKLDILKNEILSFKSISPEKAIEVSNLINEFRSKILSDFQNFEKQWFQEFKDTWKQKFIPNMERQMSWTALHETV
LEYLEKAKKEV

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 57482; Mature: 57482

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIVKKCLSVLFLLLLVAPLLYGQQSCLSDIENDFQDLKTSLKNPIFLDPAPSLNYDDR
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCC
EGKKVLMVVVHHTESPTLKSTKYALNSSGISVQLIVDRDGSITLMVPLEKRAWHAGISYA
CCCEEEEEEEEECCCCCCCCHHHEECCCCCEEEEEEECCCCEEEEEECHHHHHHCCCCHH
KVQVDNVVEELRKLNDYSIGIEIVNTGLEPFPEEQMKSVKDLILYLMKRFKIRKDMIFSH
EEHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEIGTIVYNSAIEGYAMRKPDPHKLFDWELLEKSGIGLHIGDRISPQDAEQKVNEVLYKV
HHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHH
GDKSENILKLKKRLNGFFYKILPWNDREGNMIFPDNNANYSNEFDENFAWVINQFSMHHL
CCCCHHHHHHHHHHCCEEEEEECCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHC
PKEIRKDLPLKLEQEDILPEFLVKYRNLIFSEFLSLSDKTQLSLKPPFLNEEDYKHLLSS
CHHHHHCCCCEECHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHH
LAEYENNISSDAFAMLMYKIKLYYDSYLRYNIRSSLYIPFRLNSFEKLDILKNEILSFKS
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCHHHHHHHHHHHHHHCC
ISPEKAIEVSNLINEFRSKILSDFQNFEKQWFQEFKDTWKQKFIPNMERQMSWTALHETV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LEYLEKAKKEV
HHHHHHHHHCC
>Mature Secondary Structure
MKLIVKKCLSVLFLLLLVAPLLYGQQSCLSDIENDFQDLKTSLKNPIFLDPAPSLNYDDR
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCC
EGKKVLMVVVHHTESPTLKSTKYALNSSGISVQLIVDRDGSITLMVPLEKRAWHAGISYA
CCCEEEEEEEEECCCCCCCCHHHEECCCCCEEEEEEECCCCEEEEEECHHHHHHCCCCHH
KVQVDNVVEELRKLNDYSIGIEIVNTGLEPFPEEQMKSVKDLILYLMKRFKIRKDMIFSH
EEHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEIGTIVYNSAIEGYAMRKPDPHKLFDWELLEKSGIGLHIGDRISPQDAEQKVNEVLYKV
HHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHH
GDKSENILKLKKRLNGFFYKILPWNDREGNMIFPDNNANYSNEFDENFAWVINQFSMHHL
CCCCHHHHHHHHHHCCEEEEEECCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHC
PKEIRKDLPLKLEQEDILPEFLVKYRNLIFSEFLSLSDKTQLSLKPPFLNEEDYKHLLSS
CHHHHHCCCCEECHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHH
LAEYENNISSDAFAMLMYKIKLYYDSYLRYNIRSSLYIPFRLNSFEKLDILKNEILSFKS
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCHHHHHHHHHHHHHHCC
ISPEKAIEVSNLINEFRSKILSDFQNFEKQWFQEFKDTWKQKFIPNMERQMSWTALHETV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LEYLEKAKKEV
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]