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The map label for this gene is ispH

Identifier: 190571212

GI number: 190571212

Start: 890196

End: 891122

Strand: Direct

Name: ispH

Synonym: WPa_0811

Alternate gene names: 190571212

Gene position: 890196-891122 (Clockwise)

Preceding gene: 190571211

Following gene: 190571213

Centisome position: 60.05

GC content: 33.87

Gene sequence:

>927_bases
ATGGAGATTATCTTAGCTGAACCACGCGGTTTCTGTGCAGGTGTTAAAAGAGCTGTAGACATATTAGCAATCACTTTAGA
GAAATACAAAAATGAACGCCAAGTTTATGTACTACACGAAATCGTTCATAATAAATATATAGTAGAGGGCTTTAAGAAAC
AAGGAGTGGTTTTTGTAAGCAGCATAGAAGACATTAAAGACAATAGAGGAATATTGATCTTCAGTGCACATGGAGTGTCG
AAAAGTATAGAGGATGAGGCAAAAAGAAAGGGTGTTCAAGTGATTGATGCAACATGTCCATTAGTTAGCAAAGTACATAA
AGAGGCAAAAAGGTATGAGGATAGTGGTAAAGAATTAATTCTAATTGGACATGAAAATCATCCAGAAGTTAAAGGAATTA
TAGGAAGAGTAAGTAATCCTATATCTCTAGTGCAAACTATGCAAGACGTATACAATTTACAAATCAAAGATCCAGATAAT
TTATCTTATGTAACACAAACCACGTTAAGTATTGACGATACCAAAGAAATTATTGCTACACTGAAGCTCAGATTTCCAAG
CATTACAGGCCCCGACTTAAAAGATATATGCTATGCAACACAAAATAGGCAGAACGCTGTTAAAAAATTGGCTAAAATTA
CAGACGTAGTGTTAATTGTAGGAAGCAAAAACAGTTCAAATTCAAACCGTTTATTAGACCTGTGCATTTCCACAGGAAAA
AGAGCCTATTTGATTGATAATTATAAATGTATGAACAAAAATTGGTTGCAGGGCGCAGAAAAAATAGGAATTACTGCAGG
TGCTTCTGCTCCTGATATATTAGTTGATGAGCTAGTAAATTACTTAAAAATAAATATGAATACAAAAGTTTCAGTCATGC
CAGATGGATTCACTGAAAATGTTCAGTTCAAGTATACTAAATGGTAA

Upstream 100 bases:

>100_bases
CTTGGTAATGATAAATTTGAAATTATAGTAAAGCAGTCATGATATGAAACATAGTGAGCGGAGCTTACTTTTTAGCAATG
TTTACATACTTACTTAAAAA

Downstream 100 bases:

>100_bases
TGTGCAACAACGTGCGTCAAATCAAGTCTTTAGCTTAATTCAATTTTCAGTCTGTTGGAGAATAAAATATCAAGCTGAGA
TATAATTAATCCTCGTTTGT

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MEIILAEPRGFCAGVKRAVDILAITLEKYKNERQVYVLHEIVHNKYIVEGFKKQGVVFVSSIEDIKDNRGILIFSAHGVS
KSIEDEAKRKGVQVIDATCPLVSKVHKEAKRYEDSGKELILIGHENHPEVKGIIGRVSNPISLVQTMQDVYNLQIKDPDN
LSYVTQTTLSIDDTKEIIATLKLRFPSITGPDLKDICYATQNRQNAVKKLAKITDVVLIVGSKNSSNSNRLLDLCISTGK
RAYLIDNYKCMNKNWLQGAEKIGITAGASAPDILVDELVNYLKINMNTKVSVMPDGFTENVQFKYTKW

Sequences:

>Translated_308_residues
MEIILAEPRGFCAGVKRAVDILAITLEKYKNERQVYVLHEIVHNKYIVEGFKKQGVVFVSSIEDIKDNRGILIFSAHGVS
KSIEDEAKRKGVQVIDATCPLVSKVHKEAKRYEDSGKELILIGHENHPEVKGIIGRVSNPISLVQTMQDVYNLQIKDPDN
LSYVTQTTLSIDDTKEIIATLKLRFPSITGPDLKDICYATQNRQNAVKKLAKITDVVLIVGSKNSSNSNRLLDLCISTGK
RAYLIDNYKCMNKNWLQGAEKIGITAGASAPDILVDELVNYLKINMNTKVSVMPDGFTENVQFKYTKW
>Mature_308_residues
MEIILAEPRGFCAGVKRAVDILAITLEKYKNERQVYVLHEIVHNKYIVEGFKKQGVVFVSSIEDIKDNRGILIFSAHGVS
KSIEDEAKRKGVQVIDATCPLVSKVHKEAKRYEDSGKELILIGHENHPEVKGIIGRVSNPISLVQTMQDVYNLQIKDPDN
LSYVTQTTLSIDDTKEIIATLKLRFPSITGPDLKDICYATQNRQNAVKKLAKITDVVLIVGSKNSSNSNRLLDLCISTGK
RAYLIDNYKCMNKNWLQGAEKIGITAGASAPDILVDELVNYLKINMNTKVSVMPDGFTENVQFKYTKW

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=310, Percent_Identity=49.6774193548387, Blast_Score=312, Evalue=2e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_WOLPP (B3CM01)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975570.1
- EnsemblBacteria:   EBWOLT00000000841
- GeneID:   6385579
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0811
- GeneTree:   EBGT00050000031312
- HOGENOM:   HBG335228
- OMA:   HMQVARA
- ProtClustDB:   PRK01045
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 34484; Mature: 34484

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIILAEPRGFCAGVKRAVDILAITLEKYKNERQVYVLHEIVHNKYIVEGFKKQGVVFVS
CEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHCHHHHHHHCCCCEEEEE
SIEDIKDNRGILIFSAHGVSKSIEDEAKRKGVQVIDATCPLVSKVHKEAKRYEDSGKELI
EHHHHCCCCCEEEEEECCCCCHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCCCEEE
LIGHENHPEVKGIIGRVSNPISLVQTMQDVYNLQIKDPDNLSYVTQTTLSIDDTKEIIAT
EEECCCCCHHHHHHHHCCCHHHHHHHHHHHHEEEECCCCCCEEEEEEEEECCHHHHHHHH
LKLRFPSITGPDLKDICYATQNRQNAVKKLAKITDVVLIVGSKNSSNSNRLLDLCISTGK
HHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHEEEEEEECCCCCCCHHHHHEEHHCCC
RAYLIDNYKCMNKNWLQGAEKIGITAGASAPDILVDELVNYLKINMNTKVSVMPDGFTEN
EEEEEECEEECCCHHHCHHHHCCEECCCCCCHHHHHHHHHHEEECCCCEEEECCCCCCCC
VQFKYTKW
EEEEEECC
>Mature Secondary Structure
MEIILAEPRGFCAGVKRAVDILAITLEKYKNERQVYVLHEIVHNKYIVEGFKKQGVVFVS
CEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHCHHHHHHHCCCCEEEEE
SIEDIKDNRGILIFSAHGVSKSIEDEAKRKGVQVIDATCPLVSKVHKEAKRYEDSGKELI
EHHHHCCCCCEEEEEECCCCCHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCCCEEE
LIGHENHPEVKGIIGRVSNPISLVQTMQDVYNLQIKDPDNLSYVTQTTLSIDDTKEIIAT
EEECCCCCHHHHHHHHCCCHHHHHHHHHHHHEEEECCCCCCEEEEEEEEECCHHHHHHHH
LKLRFPSITGPDLKDICYATQNRQNAVKKLAKITDVVLIVGSKNSSNSNRLLDLCISTGK
HHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHEEEEEEECCCCCCCHHHHHEEHHCCC
RAYLIDNYKCMNKNWLQGAEKIGITAGASAPDILVDELVNYLKINMNTKVSVMPDGFTEN
EEEEEECEEECCCHHHCHHHHCCEECCCCCCHHHHHHHHHHEEECCCCEEEECCCCCCCC
VQFKYTKW
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA