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The map label for this gene is priA [H]

Identifier: 190571168

GI number: 190571168

Start: 836345

End: 838309

Strand: Direct

Name: priA [H]

Synonym: WPa_0766

Alternate gene names: 190571168

Gene position: 836345-838309 (Clockwise)

Preceding gene: 190571163

Following gene: 190571169

Centisome position: 56.42

GC content: 36.54

Gene sequence:

>1965_bases
ATGAAGAAAGCAGTTGACGTATTACTACCACTTCCAATTGATCAGCTATTTTCATATGCAGTTGAGGAAGATACTGAAGT
TTCAATTGGAGATTACGTAGTGGTACCATTTGGCAGAAAGCGTTTGATTGGAATAGTTTGGAAATATAGCGGCAAGAGTG
ATCGAGAATTAAAATGCATTGAACAAAAGATCGAGTTACCGAGTATCAGACCAAAACTGATCGAATTTGCAGAGTGGGTT
GCGCAGTATAATGTAATACCTATCGGTATGCTTGCCAAAGTAATAATGGGAGGAGTATTAAAGGTAAATCAGATAGATAA
GTTAGTTTTCACTAAGCAAAAGCAGGAAATAAGTGAAATAGATTACCAATTAAGCCCTGAGCAGCAAGCAGCTCGCGATA
AGATAATAAGTAATTTGAATGAGTATTCAGTGACTTTGCTTGACGGTGAAACCGGGTCTGGAAAAACGGAAGTTTATCTC
TCTGTGATTGCAAGGTTAATTGAAATCGTAAACGATGCACAAGTCCTGATCCTTCTGCCAGAAATTGTCCTAACTTCACA
ATTAGTAAATCGTGTTCACAGTCAGATATCTGGAAATATAGCTGAGTGGCATTCAGGACTTACTCCCAAAGCTCGCCGAA
ATAATTGGCTTAATATAGCAAATGGAAGTGCGCAGATAATTATTGGGGCAAGGTCATCACTTTTTTTGCCTTATAAGAAC
CTAAAATTGATCATCGTTGATGAAGAGCATGACTCATCCTTTAAACAGGAACAAGGAATTATATATAATGCTCGAGATAT
GGCAATTATCTTAGCCAAACTCGAAAATATTCCGATTATTTTATCATCCGCTACTCCGCTGCTTGAAACAATTTATCATG
TTAAAAAAGGGAATTACAACCATGTAAAGCTAACCGAACGATTCGGTGGTGCAGAGTTGCCGCACATAAAAGTAGTGAAT
AATAAACAATGGATTTCAAATGAGCTTTTCGAAGGGATAAAACAAACCATAGAGAAAAAACAGCAAGTTATGCTCTTTCT
AAACCGCAGGGGGTATGCGCAACTTGCAGTTTGTAAAAAATGTGGATATAAAATTTCCTGCTCAAATTGCACTGTATGGC
TTACGTACCACAAGAAAAAGAACGTCCTTTTGTGTCACCATTGTTCTTACCAATCAAAACTTCCAGAAAAATGCTCGAAT
TGTCAAAGTGAGCAGTCATTATCCCTTTGTGGTATAGGAATTGAGAGGTTACTTGAAGAGATAGTAAAACTATTACCAAA
TGCAAAAACTGCAATTATCAGCAGCGATCAGAAATCGCTTGGTAGCGTCATTGACTCAGTGCTCAAGGAAGAAGTGAATA
TTATAATTGGTACACAAGTAATTGCTAAAGGGCATAATTTCCCTAAATTAACTTTGGTTGGAGTGATAAATGCAGATTTA
GGACTTGAAAATTCTGACCTCAGGGCAGCAGAAAAGACGTATCAATTATTGCATCAAGTTGCGGGTAGATCTGGGAGGTT
CAACGAAAAAGGAACGGTAATAGTGCAAACTAATAATCCTGAAAGTTTGATAATAAAAGCATTGCAGCAGCAAAAAAGGG
ATTTGTTTTATGAAATTGAACTTGAGTCAAGACGCAAAGCTAAAATGCCCCCATTTAGTAGGCTAATAGCGCTTATAGTT
TGTGGTAAGGATCAATTTGCAACGCAGAAAGCAGCAAATGAGATAACAAACTTTTTGCATAATCAATTTTCAACTACCTC
TCAGCACTTGACATTGAAAGAATTTGAAATTTTTGGCCCATCACCAGCAGCAATAAATTTCTTAAATAATAAATATCGGT
ATAGAGTGTTACTCAAAATACACAATAAATACAGTCTGTCTATAAAGAAAAAGCTGAAACATTGCTGTAACTTGAGTTCA
AATATTGAAGTAACAATAGATGTTGACCCTGTGAGTTTCTTTTGA

Upstream 100 bases:

>100_bases
ATACCTTCACCCTCAAAAAGCTAACTTAATACCAGTTTAACCAATCAGCCCACAAAAATCAAAACGAATATTTTCAAAAC
GGTGCTAAACTTAAGGTACT

Downstream 100 bases:

>100_bases
TGCGGTCGGAATTTATTTGACATTTGTAGGAATTGCTAATAAGTTCTATTAATTTTTTAATTAAAAATTTGACTTTTATT
AGAGTTTATTATATTATTGA

Product: primosomal protein N

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 654; Mature: 654

Protein sequence:

>654_residues
MKKAVDVLLPLPIDQLFSYAVEEDTEVSIGDYVVVPFGRKRLIGIVWKYSGKSDRELKCIEQKIELPSIRPKLIEFAEWV
AQYNVIPIGMLAKVIMGGVLKVNQIDKLVFTKQKQEISEIDYQLSPEQQAARDKIISNLNEYSVTLLDGETGSGKTEVYL
SVIARLIEIVNDAQVLILLPEIVLTSQLVNRVHSQISGNIAEWHSGLTPKARRNNWLNIANGSAQIIIGARSSLFLPYKN
LKLIIVDEEHDSSFKQEQGIIYNARDMAIILAKLENIPIILSSATPLLETIYHVKKGNYNHVKLTERFGGAELPHIKVVN
NKQWISNELFEGIKQTIEKKQQVMLFLNRRGYAQLAVCKKCGYKISCSNCTVWLTYHKKKNVLLCHHCSYQSKLPEKCSN
CQSEQSLSLCGIGIERLLEEIVKLLPNAKTAIISSDQKSLGSVIDSVLKEEVNIIIGTQVIAKGHNFPKLTLVGVINADL
GLENSDLRAAEKTYQLLHQVAGRSGRFNEKGTVIVQTNNPESLIIKALQQQKRDLFYEIELESRRKAKMPPFSRLIALIV
CGKDQFATQKAANEITNFLHNQFSTTSQHLTLKEFEIFGPSPAAINFLNNKYRYRVLLKIHNKYSLSIKKKLKHCCNLSS
NIEVTIDVDPVSFF

Sequences:

>Translated_654_residues
MKKAVDVLLPLPIDQLFSYAVEEDTEVSIGDYVVVPFGRKRLIGIVWKYSGKSDRELKCIEQKIELPSIRPKLIEFAEWV
AQYNVIPIGMLAKVIMGGVLKVNQIDKLVFTKQKQEISEIDYQLSPEQQAARDKIISNLNEYSVTLLDGETGSGKTEVYL
SVIARLIEIVNDAQVLILLPEIVLTSQLVNRVHSQISGNIAEWHSGLTPKARRNNWLNIANGSAQIIIGARSSLFLPYKN
LKLIIVDEEHDSSFKQEQGIIYNARDMAIILAKLENIPIILSSATPLLETIYHVKKGNYNHVKLTERFGGAELPHIKVVN
NKQWISNELFEGIKQTIEKKQQVMLFLNRRGYAQLAVCKKCGYKISCSNCTVWLTYHKKKNVLLCHHCSYQSKLPEKCSN
CQSEQSLSLCGIGIERLLEEIVKLLPNAKTAIISSDQKSLGSVIDSVLKEEVNIIIGTQVIAKGHNFPKLTLVGVINADL
GLENSDLRAAEKTYQLLHQVAGRSGRFNEKGTVIVQTNNPESLIIKALQQQKRDLFYEIELESRRKAKMPPFSRLIALIV
CGKDQFATQKAANEITNFLHNQFSTTSQHLTLKEFEIFGPSPAAINFLNNKYRYRVLLKIHNKYSLSIKKKLKHCCNLSS
NIEVTIDVDPVSFF
>Mature_654_residues
MKKAVDVLLPLPIDQLFSYAVEEDTEVSIGDYVVVPFGRKRLIGIVWKYSGKSDRELKCIEQKIELPSIRPKLIEFAEWV
AQYNVIPIGMLAKVIMGGVLKVNQIDKLVFTKQKQEISEIDYQLSPEQQAARDKIISNLNEYSVTLLDGETGSGKTEVYL
SVIARLIEIVNDAQVLILLPEIVLTSQLVNRVHSQISGNIAEWHSGLTPKARRNNWLNIANGSAQIIIGARSSLFLPYKN
LKLIIVDEEHDSSFKQEQGIIYNARDMAIILAKLENIPIILSSATPLLETIYHVKKGNYNHVKLTERFGGAELPHIKVVN
NKQWISNELFEGIKQTIEKKQQVMLFLNRRGYAQLAVCKKCGYKISCSNCTVWLTYHKKKNVLLCHHCSYQSKLPEKCSN
CQSEQSLSLCGIGIERLLEEIVKLLPNAKTAIISSDQKSLGSVIDSVLKEEVNIIIGTQVIAKGHNFPKLTLVGVINADL
GLENSDLRAAEKTYQLLHQVAGRSGRFNEKGTVIVQTNNPESLIIKALQQQKRDLFYEIELESRRKAKMPPFSRLIALIV
CGKDQFATQKAANEITNFLHNQFSTTSQHLTLKEFEIFGPSPAAINFLNNKYRYRVLLKIHNKYSLSIKKKLKHCCNLSS
NIEVTIDVDPVSFF

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=549, Percent_Identity=36.247723132969, Blast_Score=346, Evalue=2e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 73796; Mature: 73796

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKAVDVLLPLPIDQLFSYAVEEDTEVSIGDYVVVPFGRKRLIGIVWKYSGKSDRELKCI
CCCCEEEEECCCHHHHHHHHHCCCCCEECCCEEEECCCCCEEEEEEEEECCCCCCHHHHH
EQKIELPSIRPKLIEFAEWVAQYNVIPIGMLAKVIMGGVLKVNQIDKLVFTKQKQEISEI
HHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHC
DYQLSPEQQAARDKIISNLNEYSVTLLDGETGSGKTEVYLSVIARLIEIVNDAQVLILLP
CCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEHH
EIVLTSQLVNRVHSQISGNIAEWHSGLTPKARRNNWLNIANGSAQIIIGARSSLFLPYKN
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHCCCEEEEECCCEEEEEECCCCEEEEECC
LKLIIVDEEHDSSFKQEQGIIYNARDMAIILAKLENIPIILSSATPLLETIYHVKKGNYN
EEEEEEECCCCCCHHHHCCCEEECCHHEEEEEHHCCCCEEEECCCHHHHHHHHHHCCCCC
HVKLTERFGGAELPHIKVVNNKQWISNELFEGIKQTIEKKQQVMLFLNRRGYAQLAVCKK
EEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHH
CGYKISCSNCTVWLTYHKKKNVLLCHHCSYQSKLPEKCSNCQSEQSLSLCGIGIERLLEE
CCCEEEECCEEEEEEEECCCCEEEEECCCCHHCCCHHHHCCCCCCCCHHHHHHHHHHHHH
IVKLLPNAKTAIISSDQKSLGSVIDSVLKEEVNIIIGTQVIAKGHNFPKLTLVGVINADL
HHHHCCCCCEEEEECCHHHHHHHHHHHHHHHCCEEEEEEHEECCCCCCEEEEEEEEECCC
GLENSDLRAAEKTYQLLHQVAGRSGRFNEKGTVIVQTNNPESLIIKALQQQKRDLFYEIE
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEE
LESRRKAKMPPFSRLIALIVCGKDQFATQKAANEITNFLHNQFSTTSQHLTLKEFEIFGP
ECCCCCCCCCCHHHHHEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECC
SPAAINFLNNKYRYRVLLKIHNKYSLSIKKKLKHCCNLSSNIEVTIDVDPVSFF
CCHHHHCCCCCEEEEEEEEECCCEEEEHHHHHHHHHCCCCCEEEEEEECCCCCC
>Mature Secondary Structure
MKKAVDVLLPLPIDQLFSYAVEEDTEVSIGDYVVVPFGRKRLIGIVWKYSGKSDRELKCI
CCCCEEEEECCCHHHHHHHHHCCCCCEECCCEEEECCCCCEEEEEEEEECCCCCCHHHHH
EQKIELPSIRPKLIEFAEWVAQYNVIPIGMLAKVIMGGVLKVNQIDKLVFTKQKQEISEI
HHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHC
DYQLSPEQQAARDKIISNLNEYSVTLLDGETGSGKTEVYLSVIARLIEIVNDAQVLILLP
CCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEHH
EIVLTSQLVNRVHSQISGNIAEWHSGLTPKARRNNWLNIANGSAQIIIGARSSLFLPYKN
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHCCCEEEEECCCEEEEEECCCCEEEEECC
LKLIIVDEEHDSSFKQEQGIIYNARDMAIILAKLENIPIILSSATPLLETIYHVKKGNYN
EEEEEEECCCCCCHHHHCCCEEECCHHEEEEEHHCCCCEEEECCCHHHHHHHHHHCCCCC
HVKLTERFGGAELPHIKVVNNKQWISNELFEGIKQTIEKKQQVMLFLNRRGYAQLAVCKK
EEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHH
CGYKISCSNCTVWLTYHKKKNVLLCHHCSYQSKLPEKCSNCQSEQSLSLCGIGIERLLEE
CCCEEEECCEEEEEEEECCCCEEEEECCCCHHCCCHHHHCCCCCCCCHHHHHHHHHHHHH
IVKLLPNAKTAIISSDQKSLGSVIDSVLKEEVNIIIGTQVIAKGHNFPKLTLVGVINADL
HHHHCCCCCEEEEECCHHHHHHHHHHHHHHHCCEEEEEEHEECCCCCCEEEEEEEEECCC
GLENSDLRAAEKTYQLLHQVAGRSGRFNEKGTVIVQTNNPESLIIKALQQQKRDLFYEIE
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEE
LESRRKAKMPPFSRLIALIVCGKDQFATQKAANEITNFLHNQFSTTSQHLTLKEFEIFGP
ECCCCCCCCCCHHHHHEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECC
SPAAINFLNNKYRYRVLLKIHNKYSLSIKKKLKHCCNLSSNIEVTIDVDPVSFF
CCHHHHCCCCCEEEEEEEEECCCEEEEHHHHHHHHHCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]