Click here to switch to the map view.

The map label for this gene is leuS

Identifier: 190571143

GI number: 190571143

Start: 807485

End: 809944

Strand: Direct

Name: leuS

Synonym: WPa_0741

Alternate gene names: 190571143

Gene position: 807485-809944 (Clockwise)

Preceding gene: 190571142

Following gene: 190571144

Centisome position: 54.47

GC content: 36.34

Gene sequence:

>2460_bases
ATGAAATATGATTTTAAAAACGTTGAAAAATTCTACCAAAATAAGTGGGATTTTTCTGTAAGCAAAGATAGTAAAAAGAA
GAAATGTTACGTGTTGGAAATGTTTCCATATCCATCTGGTAAGATTCATATGGGGCACCTGCGTAACTATGCAATAGGAG
ATGTTATAGCACGTTACAAGAGAGCTCGTGGATTTGAGGTTTTGCACCCAATTGGCTGGGATGCGTTTGGATTACCAGCT
GAAAATGCAGCAAGGGACAATAACATTAACCCTGCAGCATGGACACAAGATAATATAGATAATATGCGCACGCAGCTAAA
ATCTATAGGTCTTTCATACAATTGGGATCGTGAGCTCTCCACATGTGAGCCTGATTATTACAAACACGAACAGAAATTTT
TTCTGGATTTTTTAAAGCATGGTCTTGCCTATCGAAAAGAGTCGTGGGTTAATTGGGATCCAGTGGACCAAACAGTGCTT
GCAAATGAGCAAGTGGTTGATGGAAAAGGATGGCGATCAGGTGCAGTTGTTGAAAAACGTAAGTTATTCCAATGGTTTTT
AAAGATTACTGATTTTGCTGAAGATTTGCTTCATTGTTTGCAAAGTTTAAAAAATTGGCCAGAAAAAGTCAAAACTATGC
AAGAGCGCTGGATAGGAAAATCTGAAGGGGCAACTATAGATTTTGAAATATTTGGCTTGAATAAGAAGTTAAAGATTTTT
ACAACTTCTCCTCATACTTTATTTGGAGCTTCTTTTCTTGCAGTAGGAGCAGAGCACCCTATTGTACAAGACCTTAAGGA
CAAAGAAATAAGAGACTTTATTGGTAATATGAAGGCAAAGGGGGAGAATGATGAAAAAATAGGAATTTACACTGGATTAA
ACGTAAAACACCCATTTCTTGATAAAGAATTACCACTTTATATAGCGAATTTTGTGTTGATGGAGTATGGAGAAGGTGCG
ATTTTTGGTTGCCCTGCACATGATCAGCGTGATTTTGAATTTGCACAGAAGTATGGTTTACCGATTATTCCTGTAGTTTG
TGAAGAGAGCACAGAGATCTTAAAAGAACCGTACTTCGGTGATGGAGTGATGTTCAATTCTGAATTCTTAAACGGACTAA
TGATTAATGAGGCAAAAAAAGTAATCATAAAAAAACTTGAAGAAAAAGGAATAGGTAAGAAAACAATAAACTATCGTCTA
CACGATTGGGGAATTTCAAGGCAACGTTATTGGGGATGTCCTATACCTATTATACATTGTAAAGATTGTGGAATTGTTCC
AGTTCCAGAAAAAGACCTCCCTGTTGTTCTACCAACGGATGTAGAATTTACAAGTGGTGGTAATCCCCTGGATAAGCACC
CAACCTGGAAATTTGTTGATTGTCCAAAGTGCGGAAAGCAAGCAGAACGTGAAACTGATACATTTGACACTTTTTTTGAA
TCTTCTTGGTATTTTGCTGCATTTTGCAGTGAAAATAAATCGATTAATAAAGATACTTGTAATCGTTTTATGCCTGTTGA
TTATTACATAGGTGGAATAGAACATGCAATTCTACATTTGCTTTACTCAAGGTTTTTCTGCCGTGCTTTAACTAAATGTG
GCTATTTTGACGTTAAAGAGCCTTTCTCTACTTTAATCACTCAAGGAATGGTCTGCCATATAACTTATAAAGATGAGAAT
GGCAAATGGCTCTTTCCCGAAGAAGCAAAAGAGTTGATTGCGAAGGGTGCTAAGATCCAAGTTGGAAAAGTCGAAAAGAT
GAGTAAGTCAAAGAAGAATACAGTTGACCCAAACTTTATCATAGAAAAATACGGTGCTGATACTGCTCGTTTGTTTGTAT
TATCTGACACTCCTCCGGAAAAAGATATGGAATGGTCTGATGATGGAGTGGAAGGTTGTTTTCGCTACATAAATAAATTG
TGGCGTATGGTAGTGCAACTTAGGACCGTAAACATACACTATGATAATGAAAACATTGTAGGTAAACTCCTAGAGTACAG
AAAAAAAATCCACAAACTCTTACACGGACTTACAGATGACTTGGAAAACTGCAGATTAAACTGTGTGGTAGCAAAGTTTC
GTGAAATGACAAATTTAATAGCTGAAATAGACGTAAAAACTGGAAAATCTCTTATTGATGAAGGTATATGCATACTAATC
AGAGTAATTGAGCCATTAATGCCACACCTGGCTGAAAATTTATGGCAAGAAATAGGAGGCGAAGGCATGCTATATATGCA
ACCTTGGCCAAAAGCTGATGAATCACTACTAATTGATAATATGGTAACTGTGGCAGTACAGATTAATGGAAAGTTGCGTG
CGACTATCAAAGTAGAAACTGATTTACCTCAAGAAGAATTGAAGAAAATAGCAACAGATTCTGTATCTAACAAAATCGAT
CAAAGCAAAATTCGCACTATATATGCTGTACCAAATAAGGTAGTAAACATAGTTATATAA

Upstream 100 bases:

>100_bases
AAATCAAGCACACAAGATAAAGATCCGCAGATGTGTTTCACAATAAGACATAATGGTCAAGCAGTCGATCCTTTAGTACA
TATAAACTGTGATTGAATTT

Downstream 100 bases:

>100_bases
AAATTTTGAGTAGCATGTCCCTAATTTCGTAGGTATACTTTTTTTGATATGCGGTTATATCTTTTTTAATATAAAGGATA
TATACTTGTGCATTATATAT

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS

Number of amino acids: Translated: 819; Mature: 819

Protein sequence:

>819_residues
MKYDFKNVEKFYQNKWDFSVSKDSKKKKCYVLEMFPYPSGKIHMGHLRNYAIGDVIARYKRARGFEVLHPIGWDAFGLPA
ENAARDNNINPAAWTQDNIDNMRTQLKSIGLSYNWDRELSTCEPDYYKHEQKFFLDFLKHGLAYRKESWVNWDPVDQTVL
ANEQVVDGKGWRSGAVVEKRKLFQWFLKITDFAEDLLHCLQSLKNWPEKVKTMQERWIGKSEGATIDFEIFGLNKKLKIF
TTSPHTLFGASFLAVGAEHPIVQDLKDKEIRDFIGNMKAKGENDEKIGIYTGLNVKHPFLDKELPLYIANFVLMEYGEGA
IFGCPAHDQRDFEFAQKYGLPIIPVVCEESTEILKEPYFGDGVMFNSEFLNGLMINEAKKVIIKKLEEKGIGKKTINYRL
HDWGISRQRYWGCPIPIIHCKDCGIVPVPEKDLPVVLPTDVEFTSGGNPLDKHPTWKFVDCPKCGKQAERETDTFDTFFE
SSWYFAAFCSENKSINKDTCNRFMPVDYYIGGIEHAILHLLYSRFFCRALTKCGYFDVKEPFSTLITQGMVCHITYKDEN
GKWLFPEEAKELIAKGAKIQVGKVEKMSKSKKNTVDPNFIIEKYGADTARLFVLSDTPPEKDMEWSDDGVEGCFRYINKL
WRMVVQLRTVNIHYDNENIVGKLLEYRKKIHKLLHGLTDDLENCRLNCVVAKFREMTNLIAEIDVKTGKSLIDEGICILI
RVIEPLMPHLAENLWQEIGGEGMLYMQPWPKADESLLIDNMVTVAVQINGKLRATIKVETDLPQEELKKIATDSVSNKID
QSKIRTIYAVPNKVVNIVI

Sequences:

>Translated_819_residues
MKYDFKNVEKFYQNKWDFSVSKDSKKKKCYVLEMFPYPSGKIHMGHLRNYAIGDVIARYKRARGFEVLHPIGWDAFGLPA
ENAARDNNINPAAWTQDNIDNMRTQLKSIGLSYNWDRELSTCEPDYYKHEQKFFLDFLKHGLAYRKESWVNWDPVDQTVL
ANEQVVDGKGWRSGAVVEKRKLFQWFLKITDFAEDLLHCLQSLKNWPEKVKTMQERWIGKSEGATIDFEIFGLNKKLKIF
TTSPHTLFGASFLAVGAEHPIVQDLKDKEIRDFIGNMKAKGENDEKIGIYTGLNVKHPFLDKELPLYIANFVLMEYGEGA
IFGCPAHDQRDFEFAQKYGLPIIPVVCEESTEILKEPYFGDGVMFNSEFLNGLMINEAKKVIIKKLEEKGIGKKTINYRL
HDWGISRQRYWGCPIPIIHCKDCGIVPVPEKDLPVVLPTDVEFTSGGNPLDKHPTWKFVDCPKCGKQAERETDTFDTFFE
SSWYFAAFCSENKSINKDTCNRFMPVDYYIGGIEHAILHLLYSRFFCRALTKCGYFDVKEPFSTLITQGMVCHITYKDEN
GKWLFPEEAKELIAKGAKIQVGKVEKMSKSKKNTVDPNFIIEKYGADTARLFVLSDTPPEKDMEWSDDGVEGCFRYINKL
WRMVVQLRTVNIHYDNENIVGKLLEYRKKIHKLLHGLTDDLENCRLNCVVAKFREMTNLIAEIDVKTGKSLIDEGICILI
RVIEPLMPHLAENLWQEIGGEGMLYMQPWPKADESLLIDNMVTVAVQINGKLRATIKVETDLPQEELKKIATDSVSNKID
QSKIRTIYAVPNKVVNIVI
>Mature_819_residues
MKYDFKNVEKFYQNKWDFSVSKDSKKKKCYVLEMFPYPSGKIHMGHLRNYAIGDVIARYKRARGFEVLHPIGWDAFGLPA
ENAARDNNINPAAWTQDNIDNMRTQLKSIGLSYNWDRELSTCEPDYYKHEQKFFLDFLKHGLAYRKESWVNWDPVDQTVL
ANEQVVDGKGWRSGAVVEKRKLFQWFLKITDFAEDLLHCLQSLKNWPEKVKTMQERWIGKSEGATIDFEIFGLNKKLKIF
TTSPHTLFGASFLAVGAEHPIVQDLKDKEIRDFIGNMKAKGENDEKIGIYTGLNVKHPFLDKELPLYIANFVLMEYGEGA
IFGCPAHDQRDFEFAQKYGLPIIPVVCEESTEILKEPYFGDGVMFNSEFLNGLMINEAKKVIIKKLEEKGIGKKTINYRL
HDWGISRQRYWGCPIPIIHCKDCGIVPVPEKDLPVVLPTDVEFTSGGNPLDKHPTWKFVDCPKCGKQAERETDTFDTFFE
SSWYFAAFCSENKSINKDTCNRFMPVDYYIGGIEHAILHLLYSRFFCRALTKCGYFDVKEPFSTLITQGMVCHITYKDEN
GKWLFPEEAKELIAKGAKIQVGKVEKMSKSKKNTVDPNFIIEKYGADTARLFVLSDTPPEKDMEWSDDGVEGCFRYINKL
WRMVVQLRTVNIHYDNENIVGKLLEYRKKIHKLLHGLTDDLENCRLNCVVAKFREMTNLIAEIDVKTGKSLIDEGICILI
RVIEPLMPHLAENLWQEIGGEGMLYMQPWPKADESLLIDNMVTVAVQINGKLRATIKVETDLPQEELKKIATDSVSNKID
QSKIRTIYAVPNKVVNIVI

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI7661872, Length=827, Percent_Identity=35.912938331318, Blast_Score=510, Evalue=1e-144,
Organism=Homo sapiens, GI5454158, Length=374, Percent_Identity=25.4010695187166, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI1786861, Length=862, Percent_Identity=43.0394431554524, Blast_Score=713, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=367, Percent_Identity=22.6158038147139, Blast_Score=84, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71997517, Length=194, Percent_Identity=50, Blast_Score=218, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI71997510, Length=194, Percent_Identity=50, Blast_Score=217, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI212645227, Length=356, Percent_Identity=28.3707865168539, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17541896, Length=410, Percent_Identity=25.3658536585366, Blast_Score=86, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6323414, Length=779, Percent_Identity=40.0513478818999, Blast_Score=555, Evalue=1e-158,
Organism=Saccharomyces cerevisiae, GI6321531, Length=377, Percent_Identity=24.6684350132626, Blast_Score=103, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6325217, Length=430, Percent_Identity=22.3255813953488, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI21355409, Length=850, Percent_Identity=34, Blast_Score=458, Evalue=1e-129,
Organism=Drosophila melanogaster, GI281366294, Length=738, Percent_Identity=22.6287262872629, Blast_Score=112, Evalue=7e-25,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYL_WOLPP (B3CLT1)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975501.1
- EnsemblBacteria:   EBWOLT00000000019
- GeneID:   6384619
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0741
- GeneTree:   EBGT00050000031015
- HOGENOM:   HBG693845
- OMA:   YARYCSP
- ProtClustDB:   PRK00390
- GO:   GO:0005737
- HAMAP:   MF_00049_B
- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11946:SF7
- PRINTS:   PR00985
- TIGRFAMs:   TIGR00396

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1; SSF47323 tRNAsyn_1a_bind; SSF50677 ValRS_IleRS_edit

EC number: =6.1.1.4

Molecular weight: Translated: 94307; Mature: 94307

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 589-589

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYDFKNVEKFYQNKWDFSVSKDSKKKKCYVLEMFPYPSGKIHMGHLRNYAIGDVIARYK
CCCCHHHHHHHHCCCCCEEECCCCCCCEEEEEEEECCCCCCEEHHHHCCHHHHHHHHHHH
RARGFEVLHPIGWDAFGLPAENAARDNNINPAAWTQDNIDNMRTQLKSIGLSYNWDRELS
HHCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCC
TCEPDYYKHEQKFFLDFLKHGLAYRKESWVNWDPVDQTVLANEQVVDGKGWRSGAVVEKR
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCEEECCCCCCCCCHHHHH
KLFQWFLKITDFAEDLLHCLQSLKNWPEKVKTMQERWIGKSEGATIDFEIFGLNKKLKIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCEEEEE
TTSPHTLFGASFLAVGAEHPIVQDLKDKEIRDFIGNMKAKGENDEKIGIYTGLNVKHPFL
ECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCC
DKELPLYIANFVLMEYGEGAIFGCPAHDQRDFEFAQKYGLPIIPVVCEESTEILKEPYFG
CCCCHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHCCCCEEEEEECCCHHHHHCCCCC
DGVMFNSEFLNGLMINEAKKVIIKKLEEKGIGKKTINYRLHDWGISRQRYWGCPIPIIHC
CCEEECHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEE
KDCGIVPVPEKDLPVVLPTDVEFTSGGNPLDKHPTWKFVDCPKCGKQAERETDTFDTFFE
CCCCCCCCCCCCCCEEECCCEEECCCCCCCCCCCCEEEEECCHHCHHHHCCCHHHHHHHC
SSWYFAAFCSENKSINKDTCNRFMPVDYYIGGIEHAILHLLYSRFFCRALTKCGYFDVKE
CCCEEEEEECCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
PFSTLITQGMVCHITYKDENGKWLFPEEAKELIAKGAKIQVGKVEKMSKSKKNTVDPNFI
HHHHHHHCCEEEEEEEECCCCCEECHHHHHHHHHCCCEEEECCHHHHHCCCCCCCCCCCE
IEKYGADTARLFVLSDTPPEKDMEWSDDGVEGCFRYINKLWRMVVQLRTVNIHYDNENIV
EEECCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEECCHHHH
GKLLEYRKKIHKLLHGLTDDLENCRLNCVVAKFREMTNLIAEIDVKTGKSLIDEGICILI
HHHHHHHHHHHHHHHHHHHHHHHCEEHHHHHHHHHHHHHHHHHCHHCCHHHHHHHHHHHH
RVIEPLMPHLAENLWQEIGGEGMLYMQPWPKADESLLIDNMVTVAVQINGKLRATIKVET
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEECCEEEEEEEECCEEEEEEEEEC
DLPQEELKKIATDSVSNKIDQSKIRTIYAVPNKVVNIVI
CCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEC
>Mature Secondary Structure
MKYDFKNVEKFYQNKWDFSVSKDSKKKKCYVLEMFPYPSGKIHMGHLRNYAIGDVIARYK
CCCCHHHHHHHHCCCCCEEECCCCCCCEEEEEEEECCCCCCEEHHHHCCHHHHHHHHHHH
RARGFEVLHPIGWDAFGLPAENAARDNNINPAAWTQDNIDNMRTQLKSIGLSYNWDRELS
HHCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCC
TCEPDYYKHEQKFFLDFLKHGLAYRKESWVNWDPVDQTVLANEQVVDGKGWRSGAVVEKR
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCEEECCCCCCCCCHHHHH
KLFQWFLKITDFAEDLLHCLQSLKNWPEKVKTMQERWIGKSEGATIDFEIFGLNKKLKIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCEEEEE
TTSPHTLFGASFLAVGAEHPIVQDLKDKEIRDFIGNMKAKGENDEKIGIYTGLNVKHPFL
ECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCC
DKELPLYIANFVLMEYGEGAIFGCPAHDQRDFEFAQKYGLPIIPVVCEESTEILKEPYFG
CCCCHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHCCCCEEEEEECCCHHHHHCCCCC
DGVMFNSEFLNGLMINEAKKVIIKKLEEKGIGKKTINYRLHDWGISRQRYWGCPIPIIHC
CCEEECHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEE
KDCGIVPVPEKDLPVVLPTDVEFTSGGNPLDKHPTWKFVDCPKCGKQAERETDTFDTFFE
CCCCCCCCCCCCCCEEECCCEEECCCCCCCCCCCCEEEEECCHHCHHHHCCCHHHHHHHC
SSWYFAAFCSENKSINKDTCNRFMPVDYYIGGIEHAILHLLYSRFFCRALTKCGYFDVKE
CCCEEEEEECCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
PFSTLITQGMVCHITYKDENGKWLFPEEAKELIAKGAKIQVGKVEKMSKSKKNTVDPNFI
HHHHHHHCCEEEEEEEECCCCCEECHHHHHHHHHCCCEEEECCHHHHHCCCCCCCCCCCE
IEKYGADTARLFVLSDTPPEKDMEWSDDGVEGCFRYINKLWRMVVQLRTVNIHYDNENIV
EEECCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEECCHHHH
GKLLEYRKKIHKLLHGLTDDLENCRLNCVVAKFREMTNLIAEIDVKTGKSLIDEGICILI
HHHHHHHHHHHHHHHHHHHHHHHCEEHHHHHHHHHHHHHHHHHCHHCCHHHHHHHHHHHH
RVIEPLMPHLAENLWQEIGGEGMLYMQPWPKADESLLIDNMVTVAVQINGKLRATIKVET
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEECCEEEEEEEECCEEEEEEEEEC
DLPQEELKKIATDSVSNKIDQSKIRTIYAVPNKVVNIVI
CCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA