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The map label for this gene is pyrF

Identifier: 190570567

GI number: 190570567

Start: 105036

End: 105716

Strand: Direct

Name: pyrF

Synonym: WPa_0101

Alternate gene names: 190570567

Gene position: 105036-105716 (Clockwise)

Preceding gene: 190570566

Following gene: 190570568

Centisome position: 7.09

GC content: 38.03

Gene sequence:

>681_bases
ATGAACCCAATAATATGCGCACTGGATACACAAGATTTGAATGAAGCCATATCTTGGGCTAATGGTCTGCGTGATAAGGT
TGGCATGGTAAAACTTGGGTTAGAATTTTTTGCTGCACATGGTCCTTCTGGAGTGAGAGAAGTTGCAAAATGCAATGTAC
CAATTTTTTTAGATCTAAAATTGTATGATATTCCAAACACTGTAGCTAGAACAGTTGAAGCAATAAAAGCTCTAGATGTT
GAAATGCTAACATTGCATATCAGTGGTGGAACAAAAATGCTTAAAGAAGCACTAAGTATAGTGCAAGGAAAGAAAATAAA
GCTAATTGGAGTAACAGTACTAACTAGTATGGGCAATGAAGATCTGAGTGAACTCGGAGTAGCAAGGGAGGCAAAATCAC
AGGTAATTTTGCTAGCAAAGCTTGCAAAAAAGATTGGACTACATGGAGTAGTTTGCTCTGCACTAGAAGCTCAAGAAGTG
CGCCAAGAATGTGGTAAAGACTTTAAAATTATTACTCCAGGAATCCGTATGAATCGAGGTCATGATGATCAAAAAAGGAC
AGCAACACCAAAAGAAGCAATAAATTCAGGAGCTGATTATATCGTAATTGGCAGACCCATTACAGAGAGTAGCAATCCCG
CAAGTAGTGCAGAGTTAATATTGAAATCCCTTACTGATTGA

Upstream 100 bases:

>100_bases
TGTTCAGTGTCTGTACAAAGCATTGCAAGTGGACCAATCCCTGAATATGGATTGTAAAATGCAACAATTACTCTTATTAT
AAAAAAAATTATACAAGATA

Downstream 100 bases:

>100_bases
AGCAGGGCAGATATAGAGTAAAAGCAACAAACGGTATGTATCCGTTCAGCAGGGTGGCAAAACAAGGTAGACGAGAAAAG
ATAACTAAATGGGTCAGTGC

Product: orotidine 5`-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MNPIICALDTQDLNEAISWANGLRDKVGMVKLGLEFFAAHGPSGVREVAKCNVPIFLDLKLYDIPNTVARTVEAIKALDV
EMLTLHISGGTKMLKEALSIVQGKKIKLIGVTVLTSMGNEDLSELGVAREAKSQVILLAKLAKKIGLHGVVCSALEAQEV
RQECGKDFKIITPGIRMNRGHDDQKRTATPKEAINSGADYIVIGRPITESSNPASSAELILKSLTD

Sequences:

>Translated_226_residues
MNPIICALDTQDLNEAISWANGLRDKVGMVKLGLEFFAAHGPSGVREVAKCNVPIFLDLKLYDIPNTVARTVEAIKALDV
EMLTLHISGGTKMLKEALSIVQGKKIKLIGVTVLTSMGNEDLSELGVAREAKSQVILLAKLAKKIGLHGVVCSALEAQEV
RQECGKDFKIITPGIRMNRGHDDQKRTATPKEAINSGADYIVIGRPITESSNPASSAELILKSLTD
>Mature_226_residues
MNPIICALDTQDLNEAISWANGLRDKVGMVKLGLEFFAAHGPSGVREVAKCNVPIFLDLKLYDIPNTVARTVEAIKALDV
EMLTLHISGGTKMLKEALSIVQGKKIKLIGVTVLTSMGNEDLSELGVAREAKSQVILLAKLAKKIGLHGVVCSALEAQEV
RQECGKDFKIITPGIRMNRGHDDQKRTATPKEAINSGADYIVIGRPITESSNPASSAELILKSLTD

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=228, Percent_Identity=42.5438596491228, Blast_Score=169, Evalue=2e-43,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_WOLPP (B3CN77)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001974925.1
- EnsemblBacteria:   EBWOLT00000000851
- GeneID:   6384331
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0101
- GeneTree:   EBGT00050000030846
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- ProtClustDB:   CLSK829488
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 24329; Mature: 24329

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 60-60 BINDING 9-9 BINDING 31-31 BINDING 115-115 BINDING 176-176 BINDING 184-184 BINDING 204-204 BINDING 205-205

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPIICALDTQDLNEAISWANGLRDKVGMVKLGLEFFAAHGPSGVREVAKCNVPIFLDLK
CCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEE
LYDIPNTVARTVEAIKALDVEMLTLHISGGTKMLKEALSIVQGKKIKLIGVTVLTSMGNE
EECCCHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHCCCEEEEEEHEEHHHCCCC
DLSELGVAREAKSQVILLAKLAKKIGLHGVVCSALEAQEVRQECGKDFKIITPGIRMNRG
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCEECCCC
HDDQKRTATPKEAINSGADYIVIGRPITESSNPASSAELILKSLTD
CCCHHHCCCHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNPIICALDTQDLNEAISWANGLRDKVGMVKLGLEFFAAHGPSGVREVAKCNVPIFLDLK
CCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEE
LYDIPNTVARTVEAIKALDVEMLTLHISGGTKMLKEALSIVQGKKIKLIGVTVLTSMGNE
EECCCHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHCCCEEEEEEHEEHHHCCCC
DLSELGVAREAKSQVILLAKLAKKIGLHGVVCSALEAQEVRQECGKDFKIITPGIRMNRG
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCEECCCC
HDDQKRTATPKEAINSGADYIVIGRPITESSNPASSAELILKSLTD
CCCHHHCCCHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA