Click here to switch to the map view.

The map label for this gene is lpdA [H]

Identifier: 190570560

GI number: 190570560

Start: 92742

End: 94121

Strand: Reverse

Name: lpdA [H]

Synonym: WPa_0093

Alternate gene names: 190570560

Gene position: 94121-92742 (Counterclockwise)

Preceding gene: 190570561

Following gene: 190570559

Centisome position: 6.35

GC content: 38.33

Gene sequence:

>1380_bases
ATGACTGATTATGATTTGATTATTATAGGTGGTGGTCCAGGTGGTTACAAATGCGCTATTGCTGCTGCAAAACTTGGACT
CAAAGTTGCTTGTATAGACAAAAATAGCATTTTTGGTGGCACGTGTTTGCGAGTTGGGTGTATACCATCAAAAGCATTAC
TCCATTCCTCTTATCAGTATGCTAGCGCGAAAAATAATCTATCAAAACTTGGCATAAAAGTTAAGGATGTAAGTCTCGAT
TTGAGAGAAATGATAGGCTATAAAGACGCTAGAGTTCAGGAGCTTGGAAAAGGCATAGAATATCTGTTTAACCTTTACAA
AATTACTAAGATCAATGGACTTGGAAAAATTACTTCTTTTGATCAAGGCAATCTTGAAGTTTCAGTTGAAGGTAAGGTAC
TGAAGACAAAAAATATAGTAATTGCAACCGGTTCTGACGTTATTTCTTTGCCAGGAATTAATATCGATGAGAAAAGTATT
ATTTCATCAACTGGTGCATTGTCTTTAACTGAAGTACCAAAAAAACTTGTCGTAATCGGAGCCGGGGCAATAGGGCTTGA
AATGTCTTCTGTATGGAGGAGGCTAGGGTCTGAAGTCACTGTAGTAGAATTTTTTGACAGAATCGCTGCAGCAATTGATG
GAGAATTAAGTAAGTCTCTACTTTCTAGTCTACAAAAACAAGGAATAAAATTTTTACTTAGCACTAAAGTTGAGGGAATA
AAACAAAGTAGTAACTCTTTGAGTGTGAAAGTTTGCTCTGTAAAAGATAATCAAACAAATACTATAGAGGCGGATAAGGT
ATTGGTGGCAGCAGGGCGCAAACCATGCTCTGAGGGTCTTGAAAAAATAGAAAAAGACAGTCGTGGCTTTATTAAAGTCA
ACAATAGTTATGAAACCAACGTAAAAGGAATATTTGCTATTGGTGATGTGATTGGTGGAGCAATGCTTGCTCACAAAGCT
GAGGAAGAAGGGGTGGCAGTTGCAGAGATACTAGCAAGGCAATTGCCTCACGTTGATTATGAAATCATACCGTCAGTCAT
TTACACCCACCCTGCAGTTTCTTCAATCGGTAAAACTGAAGAAGAACTAAAAAGTGCTGGTCGAAAGTATAAAGTTGGTA
AATGTCAATTTGCTGCAAACGGTAGAGCAAAAGTCACTGACGATGCTGAGGGATTCGTGAAAGTTCTGACTTGTAGCAAA
GCAGATACAATACTCGGTGTACATATCATAGGAGCATACGCTGATACGCTAATCAATGAAGCAGCGGTTGCAATGGCATA
TGGTGCAGCAGCAGAGGATATATACAGAATTTGTCACTCTCATCCTGATATAAATGAAGCCTTTAGGGATGCGTGCATCG
ATGCTTTCTTTAAAAAGTAA

Upstream 100 bases:

>100_bases
CCCGGTAATGTGTTTCATGCTCCAGTATTTACCCATGGTAAGATGTATATAACGACTGAAGGAAATGGTGTTTATTCTTT
AGAAAATAGGTTTGTTTTTT

Downstream 100 bases:

>100_bases
CTGTGGACCTCGGTTCAATCATTGAAAAATGGTATGATTGGCTAAAGTACAACAGGTCTTACTCACCAAACACTTTGGAG
TCATACATGAGGGACCTCAA

Product: pyruvate dehydrogenase complex, E3 component, Dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase 3; LPD-3 [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MTDYDLIIIGGGPGGYKCAIAAAKLGLKVACIDKNSIFGGTCLRVGCIPSKALLHSSYQYASAKNNLSKLGIKVKDVSLD
LREMIGYKDARVQELGKGIEYLFNLYKITKINGLGKITSFDQGNLEVSVEGKVLKTKNIVIATGSDVISLPGINIDEKSI
ISSTGALSLTEVPKKLVVIGAGAIGLEMSSVWRRLGSEVTVVEFFDRIAAAIDGELSKSLLSSLQKQGIKFLLSTKVEGI
KQSSNSLSVKVCSVKDNQTNTIEADKVLVAAGRKPCSEGLEKIEKDSRGFIKVNNSYETNVKGIFAIGDVIGGAMLAHKA
EEEGVAVAEILARQLPHVDYEIIPSVIYTHPAVSSIGKTEEELKSAGRKYKVGKCQFAANGRAKVTDDAEGFVKVLTCSK
ADTILGVHIIGAYADTLINEAAVAMAYGAAAEDIYRICHSHPDINEAFRDACIDAFFKK

Sequences:

>Translated_459_residues
MTDYDLIIIGGGPGGYKCAIAAAKLGLKVACIDKNSIFGGTCLRVGCIPSKALLHSSYQYASAKNNLSKLGIKVKDVSLD
LREMIGYKDARVQELGKGIEYLFNLYKITKINGLGKITSFDQGNLEVSVEGKVLKTKNIVIATGSDVISLPGINIDEKSI
ISSTGALSLTEVPKKLVVIGAGAIGLEMSSVWRRLGSEVTVVEFFDRIAAAIDGELSKSLLSSLQKQGIKFLLSTKVEGI
KQSSNSLSVKVCSVKDNQTNTIEADKVLVAAGRKPCSEGLEKIEKDSRGFIKVNNSYETNVKGIFAIGDVIGGAMLAHKA
EEEGVAVAEILARQLPHVDYEIIPSVIYTHPAVSSIGKTEEELKSAGRKYKVGKCQFAANGRAKVTDDAEGFVKVLTCSK
ADTILGVHIIGAYADTLINEAAVAMAYGAAAEDIYRICHSHPDINEAFRDACIDAFFKK
>Mature_458_residues
TDYDLIIIGGGPGGYKCAIAAAKLGLKVACIDKNSIFGGTCLRVGCIPSKALLHSSYQYASAKNNLSKLGIKVKDVSLDL
REMIGYKDARVQELGKGIEYLFNLYKITKINGLGKITSFDQGNLEVSVEGKVLKTKNIVIATGSDVISLPGINIDEKSII
SSTGALSLTEVPKKLVVIGAGAIGLEMSSVWRRLGSEVTVVEFFDRIAAAIDGELSKSLLSSLQKQGIKFLLSTKVEGIK
QSSNSLSVKVCSVKDNQTNTIEADKVLVAAGRKPCSEGLEKIEKDSRGFIKVNNSYETNVKGIFAIGDVIGGAMLAHKAE
EEGVAVAEILARQLPHVDYEIIPSVIYTHPAVSSIGKTEEELKSAGRKYKVGKCQFAANGRAKVTDDAEGFVKVLTCSKA
DTILGVHIIGAYADTLINEAAVAMAYGAAAEDIYRICHSHPDINEAFRDACIDAFFKK

Specific function: LPD-3 may substitute for lipoamide dehydrogenase of the 2-oxoglutarate dehydrogenase and pyruvate multienzyme complexes when the latter is inactive or missing [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=465, Percent_Identity=50.1075268817204, Blast_Score=441, Evalue=1e-124,
Organism=Homo sapiens, GI50301238, Length=463, Percent_Identity=28.9416846652268, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI291045266, Length=487, Percent_Identity=28.952772073922, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI33519430, Length=483, Percent_Identity=28.3643892339545, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI33519428, Length=483, Percent_Identity=28.3643892339545, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI33519426, Length=483, Percent_Identity=28.3643892339545, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI148277071, Length=483, Percent_Identity=28.3643892339545, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI148277065, Length=483, Percent_Identity=28.3643892339545, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI291045268, Length=475, Percent_Identity=28.6315789473684, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI22035672, Length=477, Percent_Identity=26.8343815513627, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI1786307, Length=448, Percent_Identity=37.7232142857143, Blast_Score=281, Evalue=5e-77,
Organism=Escherichia coli, GI87082354, Length=470, Percent_Identity=29.7872340425532, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI87081717, Length=467, Percent_Identity=27.6231263383298, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1789915, Length=441, Percent_Identity=26.530612244898, Blast_Score=148, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI32565766, Length=465, Percent_Identity=50.9677419354839, Blast_Score=459, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI71983419, Length=450, Percent_Identity=26.6666666666667, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71983429, Length=450, Percent_Identity=26.6666666666667, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17557007, Length=481, Percent_Identity=25.7796257796258, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71982272, Length=497, Percent_Identity=25.5533199195171, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6321091, Length=471, Percent_Identity=49.0445859872611, Blast_Score=410, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6325240, Length=469, Percent_Identity=32.409381663113, Blast_Score=231, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6325166, Length=465, Percent_Identity=28.8172043010753, Blast_Score=135, Evalue=1e-32,
Organism=Drosophila melanogaster, GI21358499, Length=465, Percent_Identity=50.752688172043, Blast_Score=444, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24640549, Length=482, Percent_Identity=28.2157676348548, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24640553, Length=482, Percent_Identity=28.2157676348548, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24640551, Length=482, Percent_Identity=28.2157676348548, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI17737741, Length=496, Percent_Identity=27.6209677419355, Blast_Score=119, Evalue=5e-27,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49048; Mature: 48917

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDYDLIIIGGGPGGYKCAIAAAKLGLKVACIDKNSIFGGTCLRVGCIPSKALLHSSYQY
CCCEEEEEEECCCCCCCCEEEEHHHCEEEEEEECCCCCCCCEEEEECCCCHHHHHCCHHH
ASAKNNLSKLGIKVKDVSLDLREMIGYKDARVQELGKGIEYLFNLYKITKINGLGKITSF
HHHHCCHHHCCEEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCC
DQGNLEVSVEGKVLKTKNIVIATGSDVISLPGINIDEKSIISSTGALSLTEVPKKLVVIG
CCCCEEEEECCEEEEECCEEEECCCCEEECCCCCCCHHHHHHCCCCEEHHHCCCEEEEEE
AGAIGLEMSSVWRRLGSEVTVVEFFDRIAAAIDGELSKSLLSSLQKQGIKFLLSTKVEGI
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQSSNSLSVKVCSVKDNQTNTIEADKVLVAAGRKPCSEGLEKIEKDSRGFIKVNNSYETN
HCCCCCEEEEEEEECCCCCCCEECCEEEEECCCCHHHHHHHHHHCCCCCEEEECCCCCCC
VKGIFAIGDVIGGAMLAHKAEEEGVAVAEILARQLPHVDYEIIPSVIYTHPAVSSIGKTE
CCEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHCCCCH
EELKSAGRKYKVGKCQFAANGRAKVTDDAEGFVKVLTCSKADTILGVHIIGAYADTLINE
HHHHHCCCEEECCEEEEECCCCCEECCCCCCEEEEEEECCCCCEEEHHHHHHHHHHHHHH
AAVAMAYGAAAEDIYRICHSHPDINEAFRDACIDAFFKK
HHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDYDLIIIGGGPGGYKCAIAAAKLGLKVACIDKNSIFGGTCLRVGCIPSKALLHSSYQY
CCEEEEEEECCCCCCCCEEEEHHHCEEEEEEECCCCCCCCEEEEECCCCHHHHHCCHHH
ASAKNNLSKLGIKVKDVSLDLREMIGYKDARVQELGKGIEYLFNLYKITKINGLGKITSF
HHHHCCHHHCCEEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCC
DQGNLEVSVEGKVLKTKNIVIATGSDVISLPGINIDEKSIISSTGALSLTEVPKKLVVIG
CCCCEEEEECCEEEEECCEEEECCCCEEECCCCCCCHHHHHHCCCCEEHHHCCCEEEEEE
AGAIGLEMSSVWRRLGSEVTVVEFFDRIAAAIDGELSKSLLSSLQKQGIKFLLSTKVEGI
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQSSNSLSVKVCSVKDNQTNTIEADKVLVAAGRKPCSEGLEKIEKDSRGFIKVNNSYETN
HCCCCCEEEEEEEECCCCCCCEECCEEEEECCCCHHHHHHHHHHCCCCCEEEECCCCCCC
VKGIFAIGDVIGGAMLAHKAEEEGVAVAEILARQLPHVDYEIIPSVIYTHPAVSSIGKTE
CCEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHCCCCH
EELKSAGRKYKVGKCQFAANGRAKVTDDAEGFVKVLTCSKADTILGVHIIGAYADTLINE
HHHHHCCCEEECCEEEEECCCCCEECCCCCCEEEEEEECCCCCEEEHHHHHHHHHHHHHH
AAVAMAYGAAAEDIYRICHSHPDINEAFRDACIDAFFKK
HHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]