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The map label for this gene is fabI [H]

Identifier: 190570537

GI number: 190570537

Start: 60223

End: 60999

Strand: Direct

Name: fabI [H]

Synonym: WPa_0066

Alternate gene names: 190570537

Gene position: 60223-60999 (Clockwise)

Preceding gene: 190570536

Following gene: 190570539

Centisome position: 4.06

GC content: 34.62

Gene sequence:

>777_bases
ATGATATTGCAGGGTAAAAAAGGGTTAATAACCGGAATAATAAATAAGAGGTCAATAGCATATGGTATAGCAAAGACTCT
CTCAGAGCACGGAGCTGAGCTTGCAATCACTTATCAAAATGAAGTAATAAAAGAGAAATTATTACCAATAGCGAATGAAT
TAAATGTGGAGCTAACACTACATTGTGATGTTTCAAATGAGGAAATCATACATAGTGCTTTTAAAGAAATAGAGGAAAAG
TGGGGTGCTATTGATTTTTTGGTACATGCAATAGCATATTCTGATAAAAATGAGCTCAATGGTAAATACGTCGATACTTC
ACTAAACAATTTTCTCAATGCAATGCATATATCGTGCTATTCTTTTACTGCTTTAGCAAAAAGCGCCGAAAAAATGATGT
TAAATGGTGGTAGTTTACTTACTTTATCTTACTATGGTGCTGAAAAAGTTATGCCAAATTATAATGTGATGGGTTTATGT
AAAGCAGCACTTGAAGCAAGTGTAAAATATATAGCATGTGATCTTGGGCCAAAGAACATCAGAGTAAATGCAATTTCTGC
TGGTCCAATCAGAACTTTGGCATCTTCTGGAATAAGTGACTTTCACTCCATATCGGAATGGAATAGAATTAATTCTCCAC
TTAGACGCAATGTTACAATAGAAGATGTTGGCAAGGCAGCACTATACTTATTAAGCGACCTGAGTAGTGGTACAACTGGA
GAAATTTTACATGTTGATTCAGGGTATAATGTTGTGGGAATGAAGATAGTAGACTAA

Upstream 100 bases:

>100_bases
GGAAATATGCGGAAGTAATTTTTATACGTTAGCTACAGTTAAAACATAATTGACTTTGCCTAGTTAATATTTCAAATTAT
AAAAAAGGCAAATAATTTCT

Downstream 100 bases:

>100_bases
TACAGTACTGCAGATGCATGTGAAGCCTGTGTTTTTTTGTTATCCGAGTAATTGACATTGGAGCCCAGTGTCAGCTACTC
GAATGACAACATTTTTTAAT

Product: enoyl-(acyl-carrier-protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 1 [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MILQGKKGLITGIINKRSIAYGIAKTLSEHGAELAITYQNEVIKEKLLPIANELNVELTLHCDVSNEEIIHSAFKEIEEK
WGAIDFLVHAIAYSDKNELNGKYVDTSLNNFLNAMHISCYSFTALAKSAEKMMLNGGSLLTLSYYGAEKVMPNYNVMGLC
KAALEASVKYIACDLGPKNIRVNAISAGPIRTLASSGISDFHSISEWNRINSPLRRNVTIEDVGKAALYLLSDLSSGTTG
EILHVDSGYNVVGMKIVD

Sequences:

>Translated_258_residues
MILQGKKGLITGIINKRSIAYGIAKTLSEHGAELAITYQNEVIKEKLLPIANELNVELTLHCDVSNEEIIHSAFKEIEEK
WGAIDFLVHAIAYSDKNELNGKYVDTSLNNFLNAMHISCYSFTALAKSAEKMMLNGGSLLTLSYYGAEKVMPNYNVMGLC
KAALEASVKYIACDLGPKNIRVNAISAGPIRTLASSGISDFHSISEWNRINSPLRRNVTIEDVGKAALYLLSDLSSGTTG
EILHVDSGYNVVGMKIVD
>Mature_258_residues
MILQGKKGLITGIINKRSIAYGIAKTLSEHGAELAITYQNEVIKEKLLPIANELNVELTLHCDVSNEEIIHSAFKEIEEK
WGAIDFLVHAIAYSDKNELNGKYVDTSLNNFLNAMHISCYSFTALAKSAEKMMLNGGSLLTLSYYGAEKVMPNYNVMGLC
KAALEASVKYIACDLGPKNIRVNAISAGPIRTLASSGISDFHSISEWNRINSPLRRNVTIEDVGKAALYLLSDLSSGTTG
EILHVDSGYNVVGMKIVD

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI40254992, Length=239, Percent_Identity=25.5230125523013, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1787545, Length=254, Percent_Identity=48.8188976377953, Blast_Score=256, Evalue=8e-70,
Organism=Escherichia coli, GI1787905, Length=249, Percent_Identity=29.718875502008, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI87082100, Length=259, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324126, Length=212, Percent_Identity=29.2452830188679, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24644337, Length=190, Percent_Identity=30, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 28160; Mature: 28160

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILQGKKGLITGIINKRSIAYGIAKTLSEHGAELAITYQNEVIKEKLLPIANELNVELTL
CCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHCCCCCCCCEEEEE
HCDVSNEEIIHSAFKEIEEKWGAIDFLVHAIAYSDKNELNGKYVDTSLNNFLNAMHISCY
EECCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHHH
SFTALAKSAEKMMLNGGSLLTLSYYGAEKVMPNYNVMGLCKAALEASVKYIACDLGPKNI
HHHHHHHHHHHHHCCCCCEEEEEECCCHHCCCCCCHHHHHHHHHHCCEEEEEEECCCCCE
RVNAISAGPIRTLASSGISDFHSISEWNRINSPLRRNVTIEDVGKAALYLLSDLSSGTTG
EEEEECCCCHHHHHHCCCHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCC
EILHVDSGYNVVGMKIVD
CEEEECCCCCEEEEEEEC
>Mature Secondary Structure
MILQGKKGLITGIINKRSIAYGIAKTLSEHGAELAITYQNEVIKEKLLPIANELNVELTL
CCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHCCCCCCCCEEEEE
HCDVSNEEIIHSAFKEIEEKWGAIDFLVHAIAYSDKNELNGKYVDTSLNNFLNAMHISCY
EECCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHHH
SFTALAKSAEKMMLNGGSLLTLSYYGAEKVMPNYNVMGLCKAALEASVKYIACDLGPKNI
HHHHHHHHHHHHHCCCCCEEEEEECCCHHCCCCCCHHHHHHHHHHCCEEEEEEECCCCCE
RVNAISAGPIRTLASSGISDFHSISEWNRINSPLRRNVTIEDVGKAALYLLSDLSSGTTG
EEEEECCCCHHHHHHCCCHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCC
EILHVDSGYNVVGMKIVD
CEEEECCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]