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The map label for this gene is bcr [H]

Identifier: 190570522

GI number: 190570522

Start: 41903

End: 43063

Strand: Direct

Name: bcr [H]

Synonym: WPa_0047

Alternate gene names: 190570522

Gene position: 41903-43063 (Clockwise)

Preceding gene: 190570519

Following gene: 190570524

Centisome position: 2.83

GC content: 35.06

Gene sequence:

>1161_bases
GTGTTACTACCGTTTTTATTAATTCTATCTCTAATTGCTAAGTTCATTGAAATTGATATTTCTGTACCTAGTTTTCCTGA
TATAGTACTCTATTTTAACGTACCGGAAGGCACAATTCAATTAACTATTGCTTATAATTTTTTAGGCGTTTGCATAGGAG
GGTTATTTTTTGGTCCATTGTCTGAATGCTATGGCAGAAGAAGGATGATGATCATAGGCAACACTTTGCTACTCATCGGT
GCTGTTGGCTGTGTTTTTGCGCTGTCAGTTTCTTGGCTTTTAATCTCTCGCTTTATTCAAGGCATTGGTGTTAGCACATC
TGTAGTTGTGTTTGCAATTATTGCAGACAGCTATAAAGGCAACGAAGCAGTAAAATTTATTGGAATCATGAATTCTATTA
TCACAGTTCTCATAGCAATTGCACCAGTATTAGGCAGTTTCATCAACAAAATTGTTGGGTGGCGTGGTAACTATGCAAGT
GTTGCAATACTTTGTTTCATCTCTTGGGTTTTGCTGCTTTTTCTGTTACCAGAAACAAAAAAGGACCGTGATATTTTTAG
TCTAAAAAAAATGATGAAAGACTATAAAAAATTATTATCAAGTTCAAAATTTATGGTGTTGTCTTTGGCACAGAGCCTTT
TTTCTGCGGCTTATATGTCATTCATTACTTGTGGCCCTTTTTTGTATATGGAAACTTTTGGTTTATCCAGCACTATTTAT
GCGCTACATCAAGGTGCAATGGTTGGGTCTTTCTCACTAATTAGCCTGTTTTCCAGTAAGATCTTAAAGAAACTTGGAGC
AATTTGGTGTGTAATTTCCGGTACAGGCGTAATTGCTATTGGATCCCTATCACTTTTAATATTCAGTATAATCATGCCCA
GTGCTTACTACTTAGTAACGCTGTCTATGGTTATTTTTTGTATTGGCTGTGGAATTTGTCAAGCAGTCATTTTCAATGCA
TCATTAAATATTTTCCCTGAAATGAAAGGCACAACTTCTTCTGCAATTTCATTTATTAGAGCTTCTATCATGGCTATTTT
TATCGGCTTAACAAGCTACGTTTACGATGGTCAAGCAACCAGTGTAGCTATTCTTGTTTTTTTTGCAGTAGTTCTAATTT
ACTGTCTATTTATTGTTTTCAAGGTCTGGAAAAATCTATAA

Upstream 100 bases:

>100_bases
CTTTATATGGGGCATTTCTACCCATTTTAATAATAGTGAAAAATGTTCTTCTTTCAACGCTTTAAATGTGATATTTTTAT
TCATTAATTAAAGATTTTAA

Downstream 100 bases:

>100_bases
TCACCATATAGATCCTTCTCATTTCTAGTTATTGCGGTAATATGTATTGTAACTTTGCTGGCTGCTCTAGATGTATCTCT
TCCAAATCACCTAATCTACC

Product: drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MLLPFLLILSLIAKFIEIDISVPSFPDIVLYFNVPEGTIQLTIAYNFLGVCIGGLFFGPLSECYGRRRMMIIGNTLLLIG
AVGCVFALSVSWLLISRFIQGIGVSTSVVVFAIIADSYKGNEAVKFIGIMNSIITVLIAIAPVLGSFINKIVGWRGNYAS
VAILCFISWVLLLFLLPETKKDRDIFSLKKMMKDYKKLLSSSKFMVLSLAQSLFSAAYMSFITCGPFLYMETFGLSSTIY
ALHQGAMVGSFSLISLFSSKILKKLGAIWCVISGTGVIAIGSLSLLIFSIIMPSAYYLVTLSMVIFCIGCGICQAVIFNA
SLNIFPEMKGTTSSAISFIRASIMAIFIGLTSYVYDGQATSVAILVFFAVVLIYCLFIVFKVWKNL

Sequences:

>Translated_386_residues
MLLPFLLILSLIAKFIEIDISVPSFPDIVLYFNVPEGTIQLTIAYNFLGVCIGGLFFGPLSECYGRRRMMIIGNTLLLIG
AVGCVFALSVSWLLISRFIQGIGVSTSVVVFAIIADSYKGNEAVKFIGIMNSIITVLIAIAPVLGSFINKIVGWRGNYAS
VAILCFISWVLLLFLLPETKKDRDIFSLKKMMKDYKKLLSSSKFMVLSLAQSLFSAAYMSFITCGPFLYMETFGLSSTIY
ALHQGAMVGSFSLISLFSSKILKKLGAIWCVISGTGVIAIGSLSLLIFSIIMPSAYYLVTLSMVIFCIGCGICQAVIFNA
SLNIFPEMKGTTSSAISFIRASIMAIFIGLTSYVYDGQATSVAILVFFAVVLIYCLFIVFKVWKNL
>Mature_386_residues
MLLPFLLILSLIAKFIEIDISVPSFPDIVLYFNVPEGTIQLTIAYNFLGVCIGGLFFGPLSECYGRRRMMIIGNTLLLIG
AVGCVFALSVSWLLISRFIQGIGVSTSVVVFAIIADSYKGNEAVKFIGIMNSIITVLIAIAPVLGSFINKIVGWRGNYAS
VAILCFISWVLLLFLLPETKKDRDIFSLKKMMKDYKKLLSSSKFMVLSLAQSLFSAAYMSFITCGPFLYMETFGLSSTIY
ALHQGAMVGSFSLISLFSSKILKKLGAIWCVISGTGVIAIGSLSLLIFSIIMPSAYYLVTLSMVIFCIGCGICQAVIFNA
SLNIFPEMKGTTSSAISFIRASIMAIFIGLTSYVYDGQATSVAILVFFAVVLIYCLFIVFKVWKNL

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=345, Percent_Identity=24.6376811594203, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1790794, Length=363, Percent_Identity=23.4159779614325, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI1787065, Length=362, Percent_Identity=24.8618784530387, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1790146, Length=244, Percent_Identity=28.6885245901639, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI48994889, Length=218, Percent_Identity=24.3119266055046, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42190; Mature: 42190

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLPFLLILSLIAKFIEIDISVPSFPDIVLYFNVPEGTIQLTIAYNFLGVCIGGLFFGPL
CHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHH
SECYGRRRMMIIGNTLLLIGAVGCVFALSVSWLLISRFIQGIGVSTSVVVFAIIADSYKG
HHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
NEAVKFIGIMNSIITVLIAIAPVLGSFINKIVGWRGNYASVAILCFISWVLLLFLLPETK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCC
KDRDIFSLKKMMKDYKKLLSSSKFMVLSLAQSLFSAAYMSFITCGPFLYMETFGLSSTIY
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHH
ALHQGAMVGSFSLISLFSSKILKKLGAIWCVISGTGVIAIGSLSLLIFSIIMPSAYYLVT
HHHCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHH
LSMVIFCIGCGICQAVIFNASLNIFPEMKGTTSSAISFIRASIMAIFIGLTSYVYDGQAT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
SVAILVFFAVVLIYCLFIVFKVWKNL
HHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLLPFLLILSLIAKFIEIDISVPSFPDIVLYFNVPEGTIQLTIAYNFLGVCIGGLFFGPL
CHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHH
SECYGRRRMMIIGNTLLLIGAVGCVFALSVSWLLISRFIQGIGVSTSVVVFAIIADSYKG
HHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
NEAVKFIGIMNSIITVLIAIAPVLGSFINKIVGWRGNYASVAILCFISWVLLLFLLPETK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCC
KDRDIFSLKKMMKDYKKLLSSSKFMVLSLAQSLFSAAYMSFITCGPFLYMETFGLSSTIY
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHH
ALHQGAMVGSFSLISLFSSKILKKLGAIWCVISGTGVIAIGSLSLLIFSIIMPSAYYLVT
HHHCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHH
LSMVIFCIGCGICQAVIFNASLNIFPEMKGTTSSAISFIRASIMAIFIGLTSYVYDGQAT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
SVAILVFFAVVLIYCLFIVFKVWKNL
HHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]