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The map label for this gene is nadB

Identifier: 18978348

GI number: 18978348

Start: 1826876

End: 1828270

Strand: Direct

Name: nadB

Synonym: PF1976

Alternate gene names: 18978348

Gene position: 1826876-1828270 (Clockwise)

Preceding gene: 18978347

Following gene: 18978349

Centisome position: 95.74

GC content: 42.44

Gene sequence:

>1395_bases
ATGAAGGTGGCAGTAGTTGGGAGTGGCCTCGCAGGTTTAACCGCTGCAATGTCATTGGCAAAAAAGGGAATTGAGGTTAC
AGTATTTGGCCCAAAACCCAAGGAATCAAATTCATATCTAGCCCAGGCGGGGATAGCGTTTCCGATAAGTGATGGAGACT
CAATTATTTCTCATGTCACAGACACCATAAGAGGGGGAAAATATCTAAACGATGTTACCGTTGTATGGAATGTAATCTCC
AAGTCAACAGAAGCCTATCACTTTCTTACTTCCCTGGGAATCGAGTTCACTAGCAGAGAGCTTGAAGGGGGCCATTCTTT
TCCTAGAATATTCACGATTAAAAACGAAACTGGAAAGTACATAATCCCCGTATTAGAAAAGCACGCAAAAGAAATGGGTG
TGAATTTCATAAGAAAATTTGCTGAGGAAGTAGCAATTCATAACAAAAAGATTGTTGGAGTTTTTGTTGAGGGAGAGCTA
CTTTATTTTGATGCGGTAATAATAGCTTCAGGAGGATTTTCTGGCTTATACAAATTCACCGCTGGCAACCCTTGGAACTT
AGGAATTCCAATAGGAGATTTAGCACTAAAGGGAGTTCCGTTGAGGGACATTGAATTCATCCAATTTCACCCAACAGGTT
TCATCGGAAAGAGAACTTACCTAATAAGTGAGGCTGTGAGGGGAGCTGGAGCAAAGCTTGTAACTGGAGAAGGCGAAAGG
TTCGTAAACGAGCTAGAGACGAGAGACGTTGTTGCTAGGGAGATATACAGAAAGATGCTCGAAGGTAAAGGGGTTTTCCT
AGATGCCACTGGAATTGAAGACTTTAAGAGGAGATTCCCATACATATACTCCTTCCTCAGAAGAGAAGGAATAAATCCAA
AAAGAGACTTAATCCCCGTAACTCCCGTGGCCCACTATACGATTGGCGGGATAAGTGTAGATATATTCTACAGAACACCC
ATCAAGGGATTGTACGCCATAGGAGAGGCAGCATGTAATGGATTCCATGGAGCGAATAGACTGGCTAGTAATTCACTTCT
AGAGTGCATAGTTTCGGGAATAGAGGTCTCAAGAACAGTTATTAGAGAGAAACCCAGGGGAGAAAGGAAAGAAGCCAAGT
ACCACGGATATGAGCCGGGAAACGTTGATGAAGTTAGAGACATAATGTGGAACCATGCAGGGATAATAAGAAGAGAGGAA
TCCTTAAGGCTTGGTCTCAAAAAATTAGAAAAGGTTGAGGCCGATCCAAGAGTAAAGATTCTGGCAGAAGCTGTCTTAAA
GTGTGCCTTAGCGAGAGAAGAAAGTAGAGGAGCTCATTACAGAGAAGACTATCCCTATTCAAGAGAGGAATTTAGAAGGC
CGAGTATTTTCCGTGTAGAAAATTGCATGCTCTAA

Upstream 100 bases:

>100_bases
TAAAGGGGAAGGAGATGTGATGAAGCTGGCCCAGTTAAGAGGGTTTCTTGGATAAAGCCCATATACCTCTACACTTTTTT
CTCTGAGAGGGAGAGGAAAA

Downstream 100 bases:

>100_bases
AAAAGTTATTAAGCACAATTCCCTAATCTTCATTGGGGATTGTTATGGAAAAGGTTGAGGAGCTTAAGAAGGAGATTGAA
AGGTTGAAAAAAGAGAGAAA

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MKVAVVGSGLAGLTAAMSLAKKGIEVTVFGPKPKESNSYLAQAGIAFPISDGDSIISHVTDTIRGGKYLNDVTVVWNVIS
KSTEAYHFLTSLGIEFTSRELEGGHSFPRIFTIKNETGKYIIPVLEKHAKEMGVNFIRKFAEEVAIHNKKIVGVFVEGEL
LYFDAVIIASGGFSGLYKFTAGNPWNLGIPIGDLALKGVPLRDIEFIQFHPTGFIGKRTYLISEAVRGAGAKLVTGEGER
FVNELETRDVVAREIYRKMLEGKGVFLDATGIEDFKRRFPYIYSFLRREGINPKRDLIPVTPVAHYTIGGISVDIFYRTP
IKGLYAIGEAACNGFHGANRLASNSLLECIVSGIEVSRTVIREKPRGERKEAKYHGYEPGNVDEVRDIMWNHAGIIRREE
SLRLGLKKLEKVEADPRVKILAEAVLKCALAREESRGAHYREDYPYSREEFRRPSIFRVENCML

Sequences:

>Translated_464_residues
MKVAVVGSGLAGLTAAMSLAKKGIEVTVFGPKPKESNSYLAQAGIAFPISDGDSIISHVTDTIRGGKYLNDVTVVWNVIS
KSTEAYHFLTSLGIEFTSRELEGGHSFPRIFTIKNETGKYIIPVLEKHAKEMGVNFIRKFAEEVAIHNKKIVGVFVEGEL
LYFDAVIIASGGFSGLYKFTAGNPWNLGIPIGDLALKGVPLRDIEFIQFHPTGFIGKRTYLISEAVRGAGAKLVTGEGER
FVNELETRDVVAREIYRKMLEGKGVFLDATGIEDFKRRFPYIYSFLRREGINPKRDLIPVTPVAHYTIGGISVDIFYRTP
IKGLYAIGEAACNGFHGANRLASNSLLECIVSGIEVSRTVIREKPRGERKEAKYHGYEPGNVDEVRDIMWNHAGIIRREE
SLRLGLKKLEKVEADPRVKILAEAVLKCALAREESRGAHYREDYPYSREEFRRPSIFRVENCML
>Mature_464_residues
MKVAVVGSGLAGLTAAMSLAKKGIEVTVFGPKPKESNSYLAQAGIAFPISDGDSIISHVTDTIRGGKYLNDVTVVWNVIS
KSTEAYHFLTSLGIEFTSRELEGGHSFPRIFTIKNETGKYIIPVLEKHAKEMGVNFIRKFAEEVAIHNKKIVGVFVEGEL
LYFDAVIIASGGFSGLYKFTAGNPWNLGIPIGDLALKGVPLRDIEFIQFHPTGFIGKRTYLISEAVRGAGAKLVTGEGER
FVNELETRDVVAREIYRKMLEGKGVFLDATGIEDFKRRFPYIYSFLRREGINPKRDLIPVTPVAHYTIGGISVDIFYRTP
IKGLYAIGEAACNGFHGANRLASNSLLECIVSGIEVSRTVIREKPRGERKEAKYHGYEPGNVDEVRDIMWNHAGIIRREE
SLRLGLKKLEKVEADPRVKILAEAVLKCALAREESRGAHYREDYPYSREEFRRPSIFRVENCML

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily

Homologues:

Organism=Homo sapiens, GI156416003, Length=513, Percent_Identity=27.8752436647173, Blast_Score=153, Evalue=3e-37,
Organism=Escherichia coli, GI1788928, Length=512, Percent_Identity=33.0078125, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1790597, Length=502, Percent_Identity=29.2828685258964, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI1786942, Length=514, Percent_Identity=26.8482490272374, Blast_Score=148, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17550100, Length=528, Percent_Identity=28.030303030303, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17505833, Length=514, Percent_Identity=26.4591439688716, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI71986328, Length=402, Percent_Identity=27.6119402985075, Blast_Score=104, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6322701, Length=512, Percent_Identity=29.1015625, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6322416, Length=507, Percent_Identity=28.7968441814596, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6320788, Length=470, Percent_Identity=27.8723404255319, Blast_Score=90, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6322511, Length=460, Percent_Identity=27.3913043478261, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17137288, Length=514, Percent_Identity=28.0155642023346, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24655642, Length=514, Percent_Identity=28.0155642023346, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24655647, Length=514, Percent_Identity=28.0155642023346, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24663005, Length=531, Percent_Identity=27.8719397363465, Blast_Score=141, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NADB_PYRFU (Q8TZL4)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_579705.1
- ProteinModelPortal:   Q8TZL4
- EnsemblBacteria:   EBPYRT00000005877
- GeneID:   1469858
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1976
- NMPDR:   fig|186497.1.peg.2028
- GeneTree:   EBGT00050000022809
- HOGENOM:   HBG293998
- OMA:   TNERAST
- ProtClustDB:   PRK08401
- BRENDA:   1.4.3.16
- GO:   GO:0005737
- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288
- Gene3D:   G3DSA:1.20.58.100
- PRINTS:   PR00368
- TIGRFAMs:   TIGR00551

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C; SSF46977 Succ_DH_flav_C

EC number: =1.4.3.16

Molecular weight: Translated: 51710; Mature: 51710

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: ACT_SITE 210-210 ACT_SITE 227-227

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAVVGSGLAGLTAAMSLAKKGIEVTVFGPKPKESNSYLAQAGIAFPISDGDSIISHVT
CEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEECCCEEEEECCCHHHHHHHH
DTIRGGKYLNDVTVVWNVISKSTEAYHFLTSLGIEFTSRELEGGHSFPRIFTIKNETGKY
HHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCHHCCHHCCCCCCCCEEEEEECCCCCE
IIPVLEKHAKEMGVNFIRKFAEEVAIHNKKIVGVFVEGELLYFDAVIIASGGFSGLYKFT
EHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECEEEEEEEEEEECCCCCCEEEEC
AGNPWNLGIPIGDLALKGVPLRDIEFIQFHPTGFIGKRTYLISEAVRGAGAKLVTGEGER
CCCCEECCCCHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEECCCHH
FVNELETRDVVAREIYRKMLEGKGVFLDATGIEDFKRRFPYIYSFLRREGINPKRDLIPV
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEC
TPVAHYTIGGISVDIFYRTPIKGLYAIGEAACNGFHGANRLASNSLLECIVSGIEVSRTV
CCHHEEEECCEEEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IREKPRGERKEAKYHGYEPGNVDEVRDIMWNHAGIIRREESLRLGLKKLEKVEADPRVKI
HHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEECHHHHHHHHHHHHHCCCCCHHHH
LAEAVLKCALAREESRGAHYREDYPYSREEFRRPSIFRVENCML
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCEEEHHCCCC
>Mature Secondary Structure
MKVAVVGSGLAGLTAAMSLAKKGIEVTVFGPKPKESNSYLAQAGIAFPISDGDSIISHVT
CEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEECCCEEEEECCCHHHHHHHH
DTIRGGKYLNDVTVVWNVISKSTEAYHFLTSLGIEFTSRELEGGHSFPRIFTIKNETGKY
HHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCHHCCHHCCCCCCCCEEEEEECCCCCE
IIPVLEKHAKEMGVNFIRKFAEEVAIHNKKIVGVFVEGELLYFDAVIIASGGFSGLYKFT
EHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECEEEEEEEEEEECCCCCCEEEEC
AGNPWNLGIPIGDLALKGVPLRDIEFIQFHPTGFIGKRTYLISEAVRGAGAKLVTGEGER
CCCCEECCCCHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEECCCHH
FVNELETRDVVAREIYRKMLEGKGVFLDATGIEDFKRRFPYIYSFLRREGINPKRDLIPV
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEC
TPVAHYTIGGISVDIFYRTPIKGLYAIGEAACNGFHGANRLASNSLLECIVSGIEVSRTV
CCHHEEEECCEEEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IREKPRGERKEAKYHGYEPGNVDEVRDIMWNHAGIIRREESLRLGLKKLEKVEADPRVKI
HHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEECHHHHHHHHHHHHHCCCCCHHHH
LAEAVLKCALAREESRGAHYREDYPYSREEFRRPSIFRVENCML
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCEEEHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA