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The map label for this gene is thiI [C]

Identifier: 18978297

GI number: 18978297

Start: 1775245

End: 1776321

Strand: Direct

Name: thiI [C]

Synonym: PF1925

Alternate gene names: 18978297

Gene position: 1775245-1776321 (Clockwise)

Preceding gene: 18978296

Following gene: 18978300

Centisome position: 93.03

GC content: 41.78

Gene sequence:

>1077_bases
ATGAAGTTCATAGTTAAAACCCAAATGGACATGGAAGCTGTGGCTGGAAACTACATCAAAGAAATTCTCCCAGACGCTAA
AGTTACAATAGCCCCAGAGGGATATCCTGGGATAGTTATAGTAGAGAGTGACAATGAGAAAGCTCTAGAAAAGATATTGG
AGGTTCCAGAGGTCGAGAAAGTTTATCCAGTTTTAATAGAAGTTCCAGCAACACTCGAGGATATTAAGGGAGCTGCAGAG
GAGATAGTAAAGTACATAAAAGAAGGCGAGAGCTTTGCTGTTAGAACAAAGAAGAGAGGAAAGAGGGACTTTTCAAGCGT
GGACGTGAACGTTGTACTTGGTGCCAGGATCAAAGATTTGACAAATGCTGAAGTGAACTTAAGCTATCCAGACAAAGTTG
TTCAAGTTGAAATAATTGGAGACAAAGCGTATATCTCAGTAATCCCAGGAGAAGAATATCTTAAGTGGAAAAAATATCCC
AAGGACAAGCCAGACTGTAGGAAACTGTTCAAGAAGCTAACAATCGTTCAGATGCCCTATTGGGGAGATTATAAAACAGC
TAGAGCTTTTGGAGAAAAGATAGGCAGGGCAGCCCAGGCCTTTGAAGTTAAAGAGCTAATAATAGCACCCAAGGAAAAGA
TGAACGCCTACGAGCTTATGGCGTTTATAAGGGGAGTAAAGGAGGGACAAGAGAGCAGATATCAAATTCAAAAGGAAGCC
TACCCCTGGGAAGTTGAAAAAGTTCCCGTAAGTGTTTGGGACCTCTACCAGGTAATTAGAGACAAGAGAAGGAGCAGGAG
GTTAATCATAATTACAGATCCAAAAGGACCTACTCTTAACGAAGTCAAAGATAAGCTTGCAAAGGACATGTATTACGCCA
AGGAAGTTGTGGTTTTCATAGGGTCAAGGGAGGGCATTCCAGTAGGCTTGTTTAGGTTTGCAGATTATGTAGTTGATTTA
GCCCCCTACATGACTTTTGCAACCGAGCATGGGATCCCAGCAACACTAGTGGCTTTGTGGACTATATATGAAGAGGAACT
TAGGAGGAGAGGTGAGATTAGTGATAAGGAAGATTAA

Upstream 100 bases:

>100_bases
TCCAAGCTAAAACTATGGATGGAAGGGACAATCATATAAGGTATGCTCTTCCCAAGGAAGAGTTAGTGAGGATAATGAAA
AAATACGGGAGGATTGAGGA

Downstream 100 bases:

>100_bases
AGAGATAATAAGGCTTTGGAGATCTAAATGTCCATTTATAAGAATTATTGAGGAGTGGAGAATGAAGAAAAAGACAAAAG
AGTTCAAGCTTAGCTAATCC

Product: hypothetical protein

Products: ThiS-COSH; AMP [C]

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MKFIVKTQMDMEAVAGNYIKEILPDAKVTIAPEGYPGIVIVESDNEKALEKILEVPEVEKVYPVLIEVPATLEDIKGAAE
EIVKYIKEGESFAVRTKKRGKRDFSSVDVNVVLGARIKDLTNAEVNLSYPDKVVQVEIIGDKAYISVIPGEEYLKWKKYP
KDKPDCRKLFKKLTIVQMPYWGDYKTARAFGEKIGRAAQAFEVKELIIAPKEKMNAYELMAFIRGVKEGQESRYQIQKEA
YPWEVEKVPVSVWDLYQVIRDKRRSRRLIIITDPKGPTLNEVKDKLAKDMYYAKEVVVFIGSREGIPVGLFRFADYVVDL
APYMTFATEHGIPATLVALWTIYEEELRRRGEISDKED

Sequences:

>Translated_358_residues
MKFIVKTQMDMEAVAGNYIKEILPDAKVTIAPEGYPGIVIVESDNEKALEKILEVPEVEKVYPVLIEVPATLEDIKGAAE
EIVKYIKEGESFAVRTKKRGKRDFSSVDVNVVLGARIKDLTNAEVNLSYPDKVVQVEIIGDKAYISVIPGEEYLKWKKYP
KDKPDCRKLFKKLTIVQMPYWGDYKTARAFGEKIGRAAQAFEVKELIIAPKEKMNAYELMAFIRGVKEGQESRYQIQKEA
YPWEVEKVPVSVWDLYQVIRDKRRSRRLIIITDPKGPTLNEVKDKLAKDMYYAKEVVVFIGSREGIPVGLFRFADYVVDL
APYMTFATEHGIPATLVALWTIYEEELRRRGEISDKED
>Mature_358_residues
MKFIVKTQMDMEAVAGNYIKEILPDAKVTIAPEGYPGIVIVESDNEKALEKILEVPEVEKVYPVLIEVPATLEDIKGAAE
EIVKYIKEGESFAVRTKKRGKRDFSSVDVNVVLGARIKDLTNAEVNLSYPDKVVQVEIIGDKAYISVIPGEEYLKWKKYP
KDKPDCRKLFKKLTIVQMPYWGDYKTARAFGEKIGRAAQAFEVKELIIAPKEKMNAYELMAFIRGVKEGQESRYQIQKEA
YPWEVEKVPVSVWDLYQVIRDKRRSRRLIIITDPKGPTLNEVKDKLAKDMYYAKEVVVFIGSREGIPVGLFRFADYVVDL
APYMTFATEHGIPATLVALWTIYEEELRRRGEISDKED

Specific function: Required For The Synthesis Of The Thiazole Moiety. [C]

COG id: COG1818

COG function: function code R; Predicted RNA-binding protein, contains THUMP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004114 [H]

Pfam domain/function: PF02926 THUMP [H]

EC number: NA

Molecular weight: Translated: 40986; Mature: 40986

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS51165 THUMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIVKTQMDMEAVAGNYIKEILPDAKVTIAPEGYPGIVIVESDNEKALEKILEVPEVEK
CCEEEECCCCHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCHHHHHHHHCCCCHHH
VYPVLIEVPATLEDIKGAAEEIVKYIKEGESFAVRTKKRGKRDFSSVDVNVVLGARIKDL
HHHEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEECHHCCCCCCCCCCEEEEECCHHHCC
TNAEVNLSYPDKVVQVEIIGDKAYISVIPGEEYLKWKKYPKDKPDCRKLFKKLTIVQMPY
CCCEEEECCCCCEEEEEEECCCEEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHEEECCC
WGDYKTARAFGEKIGRAAQAFEVKELIIAPKEKMNAYELMAFIRGVKEGQESRYQIQKEA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHCCCHHHHHHHHCC
YPWEVEKVPVSVWDLYQVIRDKRRSRRLIIITDPKGPTLNEVKDKLAKDMYYAKEVVVFI
CCEEEEECCHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHEEEEEE
GSREGIPVGLFRFADYVVDLAPYMTFATEHGIPATLVALWTIYEEELRRRGEISDKED
ECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKFIVKTQMDMEAVAGNYIKEILPDAKVTIAPEGYPGIVIVESDNEKALEKILEVPEVEK
CCEEEECCCCHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCHHHHHHHHCCCCHHH
VYPVLIEVPATLEDIKGAAEEIVKYIKEGESFAVRTKKRGKRDFSSVDVNVVLGARIKDL
HHHEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEECHHCCCCCCCCCCEEEEECCHHHCC
TNAEVNLSYPDKVVQVEIIGDKAYISVIPGEEYLKWKKYPKDKPDCRKLFKKLTIVQMPY
CCCEEEECCCCCEEEEEEECCCEEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHEEECCC
WGDYKTARAFGEKIGRAAQAFEVKELIIAPKEKMNAYELMAFIRGVKEGQESRYQIQKEA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHCCCHHHHHHHHCC
YPWEVEKVPVSVWDLYQVIRDKRRSRRLIIITDPKGPTLNEVKDKLAKDMYYAKEVVVFI
CCEEEEECCHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHEEEEEE
GSREGIPVGLFRFADYVVDLAPYMTFATEHGIPATLVALWTIYEEELRRRGEISDKED
ECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]