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The map label for this gene is frvX [C]

Identifier: 18978233

GI number: 18978233

Start: 1717470

End: 1718510

Strand: Reverse

Name: frvX [C]

Synonym: PF1861

Alternate gene names: 18978233

Gene position: 1718510-1717470 (Counterclockwise)

Preceding gene: 18978236

Following gene: 18978232

Centisome position: 90.06

GC content: 42.84

Gene sequence:

>1041_bases
ATGGTAGATTGGGAACTAATGAAAAAAATAATAGAATCTCCAGGAGTTTCTGGGTATGAACACCTGGGAATTAGAGACCT
TGTGGTAGATATTCTTAAAGATGTTGCGGATGAAGTAAAAATTGATAAGCTTGGGAATGTGATTGCCCACTTTAAGGGCT
CTGCTCCCAAGGTAATGGTTGCTGCTCACATGGATAAGATAGGACTCATGGTAAATCATATTGACAAAGATGGCTATCTA
CGTGTTGTCCCAATTGGTGGGGTTTTGCCTGAAACCTTAATAGCTCAGAAGATAAGATTCTTCACAGAAAAAGGGGAGAG
ATATGGTGTTGTAGGAGTTTTGCCTCCTCACTTGAGAAGGGAAGCCAAGGATCAAGGTGGTAAGATAGATTGGGATAGCA
TTATAGTGGATGTTGGAGCTTCTAGCAGGGAAGAAGCTGAAGAGATGGGATTCAGAATTGGGACAATTGGAGAGTTTGCA
CCAAACTTCACAAGGCTTAGCGAGCACAGGTTTGCCACCCCCTATTTGGATGATAGGATATGCCTATATGCGATGATTGA
AGCTGCTAGACAATTAGGAGAGCATGAAGCAGATATATACATTGTAGCGTCTGTGCAGGAGGAGATTGGGCTCAGAGGAG
CGAGGGTCGCGAGCTTTGCTATAGACCCAGAAGTTGGAATTGCTATGGATGTCACCTTTGCAAAGCAACCAAATGACAAA
GGAAAGATAGTTCCAGAGTTGGGTAAGGGTCCCGTTATGGATGTTGGGCCAAATATTAATCCAAAACTAAGGCAGTTTGC
TGACGAGGTTGCAAAGAAATATGAGATCCCATTACAAGTTGAACCAAGTCCAAGGCCTACTGGAACTGATGCAAATGTAA
TGCAGATAAACAGAGAAGGTGTTGCGACGGCAGTTCTCAGTATACCAATCAGATATATGCATTCCCAGGTTGAATTAGCT
GATGCTAGAGATGTTGACAATACAATAAAACTTGCTAAGGCGTTACTTGAGGAGCTAAAGCCAATGGACTTTACACCGTG
A

Upstream 100 bases:

>100_bases
GCTCTCTTTCTCTTTTTGATAAAGTCAAGTATTTTAGGCAAGCAAAGTATTTAAATATCTTTATTTGCACTGAGAAATAG
ATCTTAATGGAGGTGAAAGG

Downstream 100 bases:

>100_bases
GGGATATGATAATAGTAGTCCCAATTGGTGAAGTTCCTAGCGATGTTCTTTCTTTTCTTTCTGAAAATATTGAAAGCTTT
TACATGAAGTTTGGAATAGG

Product: endo-1,4-beta-glucanase-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MVDWELMKKIIESPGVSGYEHLGIRDLVVDILKDVADEVKIDKLGNVIAHFKGSAPKVMVAAHMDKIGLMVNHIDKDGYL
RVVPIGGVLPETLIAQKIRFFTEKGERYGVVGVLPPHLRREAKDQGGKIDWDSIIVDVGASSREEAEEMGFRIGTIGEFA
PNFTRLSEHRFATPYLDDRICLYAMIEAARQLGEHEADIYIVASVQEEIGLRGARVASFAIDPEVGIAMDVTFAKQPNDK
GKIVPELGKGPVMDVGPNINPKLRQFADEVAKKYEIPLQVEPSPRPTGTDANVMQINREGVATAVLSIPIRYMHSQVELA
DARDVDNTIKLAKALLEELKPMDFTP

Sequences:

>Translated_346_residues
MVDWELMKKIIESPGVSGYEHLGIRDLVVDILKDVADEVKIDKLGNVIAHFKGSAPKVMVAAHMDKIGLMVNHIDKDGYL
RVVPIGGVLPETLIAQKIRFFTEKGERYGVVGVLPPHLRREAKDQGGKIDWDSIIVDVGASSREEAEEMGFRIGTIGEFA
PNFTRLSEHRFATPYLDDRICLYAMIEAARQLGEHEADIYIVASVQEEIGLRGARVASFAIDPEVGIAMDVTFAKQPNDK
GKIVPELGKGPVMDVGPNINPKLRQFADEVAKKYEIPLQVEPSPRPTGTDANVMQINREGVATAVLSIPIRYMHSQVELA
DARDVDNTIKLAKALLEELKPMDFTP
>Mature_346_residues
MVDWELMKKIIESPGVSGYEHLGIRDLVVDILKDVADEVKIDKLGNVIAHFKGSAPKVMVAAHMDKIGLMVNHIDKDGYL
RVVPIGGVLPETLIAQKIRFFTEKGERYGVVGVLPPHLRREAKDQGGKIDWDSIIVDVGASSREEAEEMGFRIGTIGEFA
PNFTRLSEHRFATPYLDDRICLYAMIEAARQLGEHEADIYIVASVQEEIGLRGARVASFAIDPEVGIAMDVTFAKQPNDK
GKIVPELGKGPVMDVGPNINPKLRQFADEVAKKYEIPLQVEPSPRPTGTDANVMQINREGVATAVLSIPIRYMHSQVELA
DARDVDNTIKLAKALLEELKPMDFTP

Specific function: Hydrolyzes di-, tri- and tetrapeptides with a lysine as the N-terminal amino acid and with Gly, Lys, Ala, Phe or Glu in the second position

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family

Homologues:

Organism=Escherichia coli, GI1790332, Length=342, Percent_Identity=33.0409356725146, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1788727, Length=345, Percent_Identity=30.4347826086957, Blast_Score=151, Evalue=8e-38,
Organism=Escherichia coli, GI87082417, Length=350, Percent_Identity=29.1428571428571, Blast_Score=146, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AMPK_PYRFU (Q8TZW4)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_579590.1
- ProteinModelPortal:   Q8TZW4
- SMR:   Q8TZW4
- MEROPS:   M42.009
- EnsemblBacteria:   EBPYRT00000004116
- GeneID:   1469740
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1861
- NMPDR:   fig|186497.1.peg.1910
- GeneTree:   EBGT00050000022566
- HOGENOM:   HBG426188
- OMA:   ANVMQIN
- ProtClustDB:   CLSK253326
- InterPro:   IPR008007
- PIRSF:   PIRSF001123

Pfam domain/function: PF05343 Peptidase_M42

EC number: =3.4.11.15

Molecular weight: Translated: 38215; Mature: 38215

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: ACT_SITE 207-207

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDWELMKKIIESPGVSGYEHLGIRDLVVDILKDVADEVKIDKLGNVIAHFKGSAPKVMV
CCCHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
AAHMDKIGLMVNHIDKDGYLRVVPIGGVLPETLIAQKIRFFTEKGERYGVVGVLPPHLRR
EEHHHHHHHHEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHH
EAKDQGGKIDWDSIIVDVGASSREEAEEMGFRIGTIGEFAPNFTRLSEHRFATPYLDDRI
HHHHCCCCCCHHHEEEECCCCCHHHHHHHCCEECCHHHHCCCHHHHHHCCCCCCCCCCHH
CLYAMIEAARQLGEHEADIYIVASVQEEIGLRGARVASFAIDPEVGIAMDVTFAKQPNDK
HHHHHHHHHHHHCCCCCCEEEEEEEHHHHCCCCCEEEEEEECCCCCEEEEEEECCCCCCC
GKIVPELGKGPVMDVGPNINPKLRQFADEVAKKYEIPLQVEPSPRPTGTDANVMQINREG
CCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCEEEECCCH
VATAVLSIPIRYMHSQVELADARDVDNTIKLAKALLEELKPMDFTP
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MVDWELMKKIIESPGVSGYEHLGIRDLVVDILKDVADEVKIDKLGNVIAHFKGSAPKVMV
CCCHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
AAHMDKIGLMVNHIDKDGYLRVVPIGGVLPETLIAQKIRFFTEKGERYGVVGVLPPHLRR
EEHHHHHHHHEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHH
EAKDQGGKIDWDSIIVDVGASSREEAEEMGFRIGTIGEFAPNFTRLSEHRFATPYLDDRI
HHHHCCCCCCHHHEEEECCCCCHHHHHHHCCEECCHHHHCCCHHHHHHCCCCCCCCCCHH
CLYAMIEAARQLGEHEADIYIVASVQEEIGLRGARVASFAIDPEVGIAMDVTFAKQPNDK
HHHHHHHHHHHHCCCCCCEEEEEEEHHHHCCCCCEEEEEEECCCCCEEEEEEECCCCCCC
GKIVPELGKGPVMDVGPNINPKLRQFADEVAKKYEIPLQVEPSPRPTGTDANVMQINREG
CCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCEEEECCCH
VATAVLSIPIRYMHSQVELADARDVDNTIKLAKALLEELKPMDFTP
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA