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The map label for this gene is gltD [H]

Identifier: 18978224

GI number: 18978224

Start: 1709262

End: 1710335

Strand: Reverse

Name: gltD [H]

Synonym: PF1852

Alternate gene names: 18978224

Gene position: 1710335-1709262 (Counterclockwise)

Preceding gene: 18978226

Following gene: 18978223

Centisome position: 89.63

GC content: 38.64

Gene sequence:

>1074_bases
ATGGAAAAGAGGGTGAGCGAGATGAAGTTTTGTTTTTGTGGGGAGAAAAAAGATAGCAGAGTTAAACCACTCAGAGTAGC
TATAATAGGAGCAGGTCCAGCAGGATTATCGGCAGCATGCTCACTAGCTTGTGATGGGTTTGATGTTCATGTATATGACA
AAATGCCTGAACCTGGAGGAATGGTGGCCTTTGGTATTCCTGAGTGGAGAATTCCAATAAGTAAAGTAAGACAGGCAGTT
AAAGAAATTGAGGAGCTTGGAGTAGTGTTTCATATGAGAACAAAGGTTGTGTATGATTCTCCAAAGGAGCTTGGAGATGA
ATTTGCTGAGAGGTTCGTGTCTTTAGAGAAACTTATGAAGGAATATGACGCAATCTTAATTGCCACTGGAGCATGGCGGC
CCAGAATTTTAGATATTCCAGGGAAAGAGCTTAAAGGTGTTGAAAATGCTCTTCAACTTCTTTTAAGGATAAACTTAGCT
AGAATTGGATATCTACCTCAAACAAAAATGCCTTCCTTCGAGGGAAAGAGGGTTGTAGTAATTGGTGCAGGTTATACTGC
AGTTGACGTTGCTAAAGAAGCAAAGTTGCTGGGGGCAAAATCAGTAACGATTGCATATAGAAGGAGTCTACAGGATTCAT
ACGCAAAAAATGAGTTTAAAAAACTTATAAAAGAGGGAGTTAAGTTCCTTGAATATGTAACTCCTGTAAGGATTTTTGGA
GATGAAAATGTTAGAGAAGTTGAATTTGCTAAAACAAAGGTAGTTAGAGGATCTGTGATTACAACAGAAGAGAAAAGAAC
TATCCCTGCAGATGTCATTATATTTGCAATAGGTCAAATCCCCACAAATCCTATAAAAGAAGTTCTATGTTCGAATGAGG
ATATTCTCAAAAATGCTGGAATATTTTTCGCTGGAGACGTTATATCTCCCAGGAATATAGGAACAGCAATTAGAGAGGGT
AGAAAAGTAGCTGAAGAGATAAAAGCATGGTTAACTGAGCATGCATTCCTAAATATCAGACTTTCTTATCCTGTTTCCCT
TAGAGACATTTCGAAGAGCGCAACTCAGTGTTAG

Upstream 100 bases:

>100_bases
TAAATTGTCTTAGAGAGAATGAAATTAGATGGATGATCACTATATATATTTGTCAAATTGACTATTGTAATTTGGATAAC
CTAAATTAGCTAGGTTTCTT

Downstream 100 bases:

>100_bases
ATAGATTTTATAAACTTTTCTTCCTAATCTTTAAATGATGGACAAAGAGCAACTTACGACAGTAGTTGAAAAGATGTTGA
ATAGTATAGGGTTTAAAACT

Product: putative glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MEKRVSEMKFCFCGEKKDSRVKPLRVAIIGAGPAGLSAACSLACDGFDVHVYDKMPEPGGMVAFGIPEWRIPISKVRQAV
KEIEELGVVFHMRTKVVYDSPKELGDEFAERFVSLEKLMKEYDAILIATGAWRPRILDIPGKELKGVENALQLLLRINLA
RIGYLPQTKMPSFEGKRVVVIGAGYTAVDVAKEAKLLGAKSVTIAYRRSLQDSYAKNEFKKLIKEGVKFLEYVTPVRIFG
DENVREVEFAKTKVVRGSVITTEEKRTIPADVIIFAIGQIPTNPIKEVLCSNEDILKNAGIFFAGDVISPRNIGTAIREG
RKVAEEIKAWLTEHAFLNIRLSYPVSLRDISKSATQC

Sequences:

>Translated_357_residues
MEKRVSEMKFCFCGEKKDSRVKPLRVAIIGAGPAGLSAACSLACDGFDVHVYDKMPEPGGMVAFGIPEWRIPISKVRQAV
KEIEELGVVFHMRTKVVYDSPKELGDEFAERFVSLEKLMKEYDAILIATGAWRPRILDIPGKELKGVENALQLLLRINLA
RIGYLPQTKMPSFEGKRVVVIGAGYTAVDVAKEAKLLGAKSVTIAYRRSLQDSYAKNEFKKLIKEGVKFLEYVTPVRIFG
DENVREVEFAKTKVVRGSVITTEEKRTIPADVIIFAIGQIPTNPIKEVLCSNEDILKNAGIFFAGDVISPRNIGTAIREG
RKVAEEIKAWLTEHAFLNIRLSYPVSLRDISKSATQC
>Mature_357_residues
MEKRVSEMKFCFCGEKKDSRVKPLRVAIIGAGPAGLSAACSLACDGFDVHVYDKMPEPGGMVAFGIPEWRIPISKVRQAV
KEIEELGVVFHMRTKVVYDSPKELGDEFAERFVSLEKLMKEYDAILIATGAWRPRILDIPGKELKGVENALQLLLRINLA
RIGYLPQTKMPSFEGKRVVVIGAGYTAVDVAKEAKLLGAKSVTIAYRRSLQDSYAKNEFKKLIKEGVKFLEYVTPVRIFG
DENVREVEFAKTKVVRGSVITTEEKRTIPADVIIFAIGQIPTNPIKEVLCSNEDILKNAGIFFAGDVISPRNIGTAIREG
RKVAEEIKAWLTEHAFLNIRLSYPVSLRDISKSATQC

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI1789606, Length=336, Percent_Identity=31.547619047619, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1788811, Length=336, Percent_Identity=31.547619047619, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI87082180, Length=267, Percent_Identity=32.9588014981273, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1788468, Length=317, Percent_Identity=30.9148264984227, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1789243, Length=322, Percent_Identity=29.1925465838509, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17570289, Length=355, Percent_Identity=25.9154929577465, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17543860, Length=178, Percent_Identity=28.6516853932584, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320030, Length=177, Percent_Identity=31.0734463276836, Blast_Score=83, Evalue=7e-17,
Organism=Drosophila melanogaster, GI28574881, Length=348, Percent_Identity=28.448275862069, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24665539, Length=348, Percent_Identity=28.448275862069, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24665547, Length=348, Percent_Identity=28.448275862069, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24665543, Length=348, Percent_Identity=28.448275862069, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24640763, Length=268, Percent_Identity=27.2388059701493, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI18858217, Length=268, Percent_Identity=27.2388059701493, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 39723; Mature: 39723

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKRVSEMKFCFCGEKKDSRVKPLRVAIIGAGPAGLSAACSLACDGFDVHVYDKMPEPGG
CCCHHHHHHEEECCCCCCCCCCEEEEEEEECCCCCHHHHHHHCCCCEEEEEEECCCCCCC
MVAFGIPEWRIPISKVRQAVKEIEELGVVFHMRTKVVYDSPKELGDEFAERFVSLEKLMK
EEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHH
EYDAILIATGAWRPRILDIPGKELKGVENALQLLLRINLARIGYLPQTKMPSFEGKRVVV
HCCEEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEE
IGAGYTAVDVAKEAKLLGAKSVTIAYRRSLQDSYAKNEFKKLIKEGVKFLEYVTPVRIFG
EECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEC
DENVREVEFAKTKVVRGSVITTEEKRTIPADVIIFAIGQIPTNPIKEVLCSNEDILKNAG
CCCCHHHHHHHHHEEECCEEECCCCCCCCHHEEEEEECCCCCCHHHHHHCCCCHHHHCCC
IFFAGDVISPRNIGTAIREGRKVAEEIKAWLTEHAFLNIRLSYPVSLRDISKSATQC
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCEEHHHHHHHHCCC
>Mature Secondary Structure
MEKRVSEMKFCFCGEKKDSRVKPLRVAIIGAGPAGLSAACSLACDGFDVHVYDKMPEPGG
CCCHHHHHHEEECCCCCCCCCCEEEEEEEECCCCCHHHHHHHCCCCEEEEEEECCCCCCC
MVAFGIPEWRIPISKVRQAVKEIEELGVVFHMRTKVVYDSPKELGDEFAERFVSLEKLMK
EEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHH
EYDAILIATGAWRPRILDIPGKELKGVENALQLLLRINLARIGYLPQTKMPSFEGKRVVV
HCCEEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEE
IGAGYTAVDVAKEAKLLGAKSVTIAYRRSLQDSYAKNEFKKLIKEGVKFLEYVTPVRIFG
EECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEC
DENVREVEFAKTKVVRGSVITTEEKRTIPADVIIFAIGQIPTNPIKEVLCSNEDILKNAG
CCCCHHHHHHHHHEEECCEEECCCCCCCCHHEEEEEECCCCCCHHHHHHCCCCHHHHCCC
IFFAGDVISPRNIGTAIREGRKVAEEIKAWLTEHAFLNIRLSYPVSLRDISKSATQC
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCEEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]