Click here to switch to the map view.

The map label for this gene is pfpI

Identifier: 18978091

GI number: 18978091

Start: 1598702

End: 1599202

Strand: Direct

Name: pfpI

Synonym: PF1719

Alternate gene names: 18978091

Gene position: 1598702-1599202 (Clockwise)

Preceding gene: 18978086

Following gene: 18978092

Centisome position: 83.78

GC content: 44.51

Gene sequence:

>501_bases
ATGAAGATACTGTTCTTGAGTGCAAACGAATTTGAAGATGTAGAGCTAATCTACCCCTACCACAGGCTCAAGGAAGAAGG
TCACGAAGTTTACATAGCGAGCTTCGAGAAGGGTGTAATTACAGGAAAGCACGGATATTCAGTCAAGGTGGACTTAACCT
TTGACGAGGTTAACCCCGACGAATTCGACGCTTTAGTTCTCCCAGGAGGAAGGGCCCCAGAGAGAGTAAGGCTTAACGAG
AAAGCAGTTGAGATAGCAAGAAAAATGTTCACCGAAGGAAAGCCAGTGGCAACTATCTGTCATGGTCCTCAGATATTGAT
TTCCGCTGGAGTGCTAAAGGGAAGAAAGGGAACAAGCTACATAGGAATAAGAGACGACATGATTAATGCCGGAGTAGAGT
GGATAGACAGAGAGGTTGTTGTTGACGGAAACTGGGTTAGTTCTAGACACCCAGGAGACCTTTACGCTTGGATGAGAGAA
TTTGTTAAATTACTTAAGTGA

Upstream 100 bases:

>100_bases
ATTTAACCAACAACATTTTTTGAAAACTAATTCATTAAACAGTATTGTTAACCCAAAATGCCTTAAAGAAAAGCCACGAA
TAAAGTCTTTGGTGATAGGA

Downstream 100 bases:

>100_bases
TCATTGTAAGTATTCATAATTCATAAATTTTTTATATTTTTGTTAGTCCTTTTTCATTGGTGAATACTAGTGAGAAGGGG
GCTATCATTACTTGGTTTTA

Product: intracellular protease

Products: NA

Alternate protein names: Intracellular protease I

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MKILFLSANEFEDVELIYPYHRLKEEGHEVYIASFEKGVITGKHGYSVKVDLTFDEVNPDEFDALVLPGGRAPERVRLNE
KAVEIARKMFTEGKPVATICHGPQILISAGVLKGRKGTSYIGIRDDMINAGVEWIDREVVVDGNWVSSRHPGDLYAWMRE
FVKLLK

Sequences:

>Translated_166_residues
MKILFLSANEFEDVELIYPYHRLKEEGHEVYIASFEKGVITGKHGYSVKVDLTFDEVNPDEFDALVLPGGRAPERVRLNE
KAVEIARKMFTEGKPVATICHGPQILISAGVLKGRKGTSYIGIRDDMINAGVEWIDREVVVDGNWVSSRHPGDLYAWMRE
FVKLLK
>Mature_166_residues
MKILFLSANEFEDVELIYPYHRLKEEGHEVYIASFEKGVITGKHGYSVKVDLTFDEVNPDEFDALVLPGGRAPERVRLNE
KAVEIARKMFTEGKPVATICHGPQILISAGVLKGRKGTSYIGIRDDMINAGVEWIDREVVVDGNWVSSRHPGDLYAWMRE
FVKLLK

Specific function: Highly stable protease. Although the physiological function is unclear it is the dominant protease in P.furiosus

COG id: COG0693

COG function: function code R; Putative intracellular protease/amidase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PfpI endopeptidase domain

Homologues:

Organism=Escherichia coli, GI87082219, Length=167, Percent_Identity=47.3053892215569, Blast_Score=142, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17558714, Length=166, Percent_Identity=24.6987951807229, Blast_Score=66, Evalue=7e-12,
Organism=Drosophila melanogaster, GI28571932, Length=156, Percent_Identity=29.4871794871795, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PFPI_PYRFU (Q51732)

Other databases:

- EMBL:   U57642
- EMBL:   AE009950
- PIR:   JC6003
- RefSeq:   NP_579448.1
- ProteinModelPortal:   Q51732
- SMR:   Q51732
- MEROPS:   C56.001
- EnsemblBacteria:   EBPYRT00000004875
- GeneID:   1469596
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1719
- NMPDR:   fig|186497.1.peg.1767
- GeneTree:   EBGT00050000022401
- HOGENOM:   HBG646910
- OMA:   VATICHG
- ProtClustDB:   CLSK253649
- GO:   GO:0005737
- InterPro:   IPR006286
- InterPro:   IPR002818
- TIGRFAMs:   TIGR01382

Pfam domain/function: PF01965 DJ-1_PfpI

EC number: 3.2.-.-

Molecular weight: Translated: 18791; Mature: 18791

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS51276 PEPTIDASE_C56_PFPI

Important sites: ACT_SITE 100-100 ACT_SITE 101-101

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILFLSANEFEDVELIYPYHRLKEEGHEVYIASFEKGVITGKHGYSVKVDLTFDEVNPD
CEEEEEECCCCCCEEEECCHHHHHCCCCEEEEEEECCCEEECCCCCEEEEEEEECCCCCC
EFDALVLPGGRAPERVRLNEKAVEIARKMFTEGKPVATICHGPQILISAGVLKGRKGTSY
CCCEEEECCCCCCCEEECCHHHHHHHHHHHHCCCCEEEEECCCEEEEEECEECCCCCCEE
IGIRDDMINAGVEWIDREVVVDGNWVSSRHPGDLYAWMREFVKLLK
EEECHHHHHCCHHHCCCEEEEECCEECCCCCCHHHHHHHHHHHHHC
>Mature Secondary Structure
MKILFLSANEFEDVELIYPYHRLKEEGHEVYIASFEKGVITGKHGYSVKVDLTFDEVNPD
CEEEEEECCCCCCEEEECCHHHHHCCCCEEEEEEECCCEEECCCCCEEEEEEEECCCCCC
EFDALVLPGGRAPERVRLNEKAVEIARKMFTEGKPVATICHGPQILISAGVLKGRKGTSY
CCCEEEECCCCCCCEEECCHHHHHHHHHHHHCCCCEEEEECCCEEEEEECEECCCCCCEE
IGIRDDMINAGVEWIDREVVVDGNWVSSRHPGDLYAWMREFVKLLK
EEECHHHHHCCHHHCCCEEEEECCEECCCCCCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8626329