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The map label for this gene is pdxT

Identifier: 18977900

GI number: 18977900

Start: 1425303

End: 1425896

Strand: Reverse

Name: pdxT

Synonym: PF1528

Alternate gene names: 18977900

Gene position: 1425896-1425303 (Counterclockwise)

Preceding gene: 18977901

Following gene: 18977899

Centisome position: 74.72

GC content: 43.27

Gene sequence:

>594_bases
ATGGTCAAGATAGGTGTTATTGGCCTTCAGGGAGATGTAAGCGAGCACATTGAAGCTACTAAAAGGGCCTTGGAAAGATT
AGGGATTGAAGGGAGTGTTATATGGGTCAAGAGACCCGAACAACTCAACCAAATTGATGGAGTAATAATCCCAGGAGGGG
AAAGCACAACAATCTCAAGACTAATGCAGAGAACAGGATTATTTGATCCATTAAAAAAGATGATTGAGGATGGCCTCCCC
GCAATGGGTACTTGTGCAGGGCTGATAATGCTTGCAAAGGAAGTTATTGGAGCTACACCAGAGCAAAAGTTCCTTGAGGT
TCTTGATGTGAAGGTGAACAGGAATGCCTATGGTAGGCAAGTTGACAGCTTTGAAGCTCCTGTAAAGTTGGCATTTGACG
ATAAACCATTCATTGGTGTTTTCATTAGGGCTCCGAGGATAGTTGAGCTTTTGTCAGACAAGGTTAAGCCCCTTGCTTGG
CTGGAAGATAGAGTTGTAGGGGTTGAACAAGGAAACGTTATCGGTCTAGAATTCCATCCCGAGCTTACTGACGATACTAG
AATTCACGAGTATTTCCTAAAGAAGATTGTCTAA

Upstream 100 bases:

>100_bases
ATTGGTGAACCAATGCGTGGGCAAGATATAGCGGAGTTAGAAGTTAGAATGGAAGAGAGGGGAGTTTGAATTTCTCTTCT
CTATTTTTTGAGGTGATTTA

Downstream 100 bases:

>100_bases
ATTATTTTGGAATTTTATTTTTGTCCTTAACTTAATTAAGTTCTCAGTTATACCTACCTATGTTCTGTTATATATCTGTA
CAATGAAAATTTTATAAGGA

Product: glutamine amidotransferase subunit PdxT

Products: AMP; L-Glutamate; GMP; H+; Diphosphate [C]

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MVKIGVIGLQGDVSEHIEATKRALERLGIEGSVIWVKRPEQLNQIDGVIIPGGESTTISRLMQRTGLFDPLKKMIEDGLP
AMGTCAGLIMLAKEVIGATPEQKFLEVLDVKVNRNAYGRQVDSFEAPVKLAFDDKPFIGVFIRAPRIVELLSDKVKPLAW
LEDRVVGVEQGNVIGLEFHPELTDDTRIHEYFLKKIV

Sequences:

>Translated_197_residues
MVKIGVIGLQGDVSEHIEATKRALERLGIEGSVIWVKRPEQLNQIDGVIIPGGESTTISRLMQRTGLFDPLKKMIEDGLP
AMGTCAGLIMLAKEVIGATPEQKFLEVLDVKVNRNAYGRQVDSFEAPVKLAFDDKPFIGVFIRAPRIVELLSDKVKPLAW
LEDRVVGVEQGNVIGLEFHPELTDDTRIHEYFLKKIV
>Mature_197_residues
MVKIGVIGLQGDVSEHIEATKRALERLGIEGSVIWVKRPEQLNQIDGVIIPGGESTTISRLMQRTGLFDPLKKMIEDGLP
AMGTCAGLIMLAKEVIGATPEQKFLEVLDVKVNRNAYGRQVDSFEAPVKLAFDDKPFIGVFIRAPRIVELLSDKVKPLAW
LEDRVVGVEQGNVIGLEFHPELTDDTRIHEYFLKKIV

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family

Homologues:

Organism=Saccharomyces cerevisiae, GI6323742, Length=212, Percent_Identity=41.9811320754717, Blast_Score=129, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6321048, Length=221, Percent_Identity=36.1990950226244, Blast_Score=117, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6323995, Length=221, Percent_Identity=35.7466063348416, Blast_Score=115, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXT_PYRFU (Q8TH23)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_579257.1
- ProteinModelPortal:   Q8TH23
- SMR:   Q8TH23
- EnsemblBacteria:   EBPYRT00000005908
- GeneID:   1469404
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1528
- NMPDR:   fig|186497.1.peg.1575
- GeneTree:   EBGT00050000023184
- HOGENOM:   HBG292341
- OMA:   QGDVREH
- ProtClustDB:   PRK13527
- HAMAP:   MF_01615
- InterPro:   IPR002161
- InterPro:   IPR021196
- PIRSF:   PIRSF005639
- TIGRFAMs:   TIGR03800

Pfam domain/function: PF01174 SNO

EC number: 6.3.5.2 [C]

Molecular weight: Translated: 21901; Mature: 21901

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS01236 PDXT_SNO_1; PS51130 PDXT_SNO_2

Important sites: ACT_SITE 85-85 ACT_SITE 179-179 ACT_SITE 181-181 BINDING 114-114

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKIGVIGLQGDVSEHIEATKRALERLGIEGSVIWVKRPEQLNQIDGVIIPGGESTTISR
CEEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHCCCEEECCCCCHHHHH
LMQRTGLFDPLKKMIEDGLPAMGTCAGLIMLAKEVIGATPEQKFLEVLDVKVNRNAYGRQ
HHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCC
VDSFEAPVKLAFDDKPFIGVFIRAPRIVELLSDKVKPLAWLEDRVVGVEQGNVIGLEFHP
CCCCCCCEEEEECCCCCEEEEECCHHHHHHHHHCCCHHHHHHHHEEEECCCCEEEEEECC
ELTDDTRIHEYFLKKIV
CCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MVKIGVIGLQGDVSEHIEATKRALERLGIEGSVIWVKRPEQLNQIDGVIIPGGESTTISR
CEEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHCCCEEECCCCCHHHHH
LMQRTGLFDPLKKMIEDGLPAMGTCAGLIMLAKEVIGATPEQKFLEVLDVKVNRNAYGRQ
HHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCC
VDSFEAPVKLAFDDKPFIGVFIRAPRIVELLSDKVKPLAWLEDRVVGVEQGNVIGLEFHP
CCCCCCCEEEEECCCCCEEEEECCHHHHHHHHHCCCHHHHHHHHEEEECCCCEEEEEECC
ELTDDTRIHEYFLKKIV
CCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1 {NH4+}} 0.42 {6-thioXMP}} 0.53 {ATP}} 2 {1-ribosyl-4,6-dihydroxypyrazolo[3,4-d]-pyrimidine} 0.058 {XMP}} 0.029 {XMP}} 0.35 {8-azaXMP}} [C]

Substrates: ATP; L-Glutamine; H2O; Xanthosine 5'-phosphate [C]

Specific reaction: ATP + L-Glutamine + H2O + Xanthosine 5'-phosphate --> AMP + L-Glutamate + GMP + (2) H+ + Diphosphate [C]

General reaction: Amination [C]

Inhibitor: 1, N6-Ethenoadenosine; 1-Methyl adenosine 5'-phosphate 5'-phosphate; 2', 3'-Dihydroadenosine; 2'-Deoxyadenosine; 2, 6-Diaminopurine ribonucleoside; 2-Amino-6-methyl thiopurine ribonucleoside; 2-Fluoroadenosine; 3'-Deoxyadenosine; 5'-Azido-5'-deoxyadenosin

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA