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The map label for this gene is hycD [H]

Identifier: 18977807

GI number: 18977807

Start: 1344053

End: 1345018

Strand: Direct

Name: hycD [H]

Synonym: PF1435

Alternate gene names: 18977807

Gene position: 1344053-1345018 (Clockwise)

Preceding gene: 18977806

Following gene: 18977808

Centisome position: 70.43

GC content: 42.24

Gene sequence:

>966_bases
ATGAAAATTGTATATGGAGTTATTGGCTTAATCCTCATTTACATTTACGTTTCCGTAGTTTCACTTTTATTTAGTGGTAT
AGATAGAAAGCTTGTAGCTAGAATGCAGAGGAGAATAGGGCCTCCAATTTTACAGCCCTTCTATGACTTTCTTAAGCTCA
TGAGCAAGGAAACAATAATACCTAAGACAGCAAACTTCATGTTTAAGGCAGCTCCAATTTTAATGCTTGCGACAGTAATA
GCACTTTTAGCCTACACCCCTCTAGGATTTCCACCAATATTTGGGACAAAGGGAGATATAATAGTCTTCATATATCTTTT
GACCTTAGCTGACTTCTTCTTAGTTGTGGGTGTCATGAGCTCAGGGAGTCCCTACGGAAGAATAGGTGCCGCAAGAGGGA
TCGCACTATTAGTGTCAAGAGAGCCAGCGATGATGCTTGGAGTATTTGCGGTAATGTGGGCGATATCAAAACTTGGTGTT
GAAAAACCCTTCAGCTTATCGAGCCTATATGAACACACCATATGGGATTTTGGTCCAGTCGCTTGGGTCGCTGGAGTTGT
ATTAATATACGTCTTCATGGCTTGGCTAGCCAGCGAGATAGAGGTTGGGTTCTTTAACATACCAGAGGCAGAACAAGAAA
TTGCAGAGGGGACTTTAGTAGAGTACAGTGGAAGATACCTGGGCATAATAAAACTTGCCGAGTCTATTAAAGAGTTTATA
GCGGCTTCACTAGTAGTTGCAGTTTTATTCCCATGGCAACTTAACATCCCAGGTGTTCAGGGATACCTAATAAACCTCTT
GCTCCACACGCTAAAGGTGTTCATCGTTCTTCTAGTCTCCAAGACGATATTCAGAACTATAACAGGCAGACTGAAGATCA
GCCAAGCAGTAAATCTCCTCTGGACTAGGGTGTTTACCGCGAGTGTTATTGGTGCTCTACTTCTTGCATTGGGGGTGATG
CTATGA

Upstream 100 bases:

>100_bases
ATCTATTAAAGCTTTCTATTGAAAAGACAAAGGAGATCAACCCAAACGTAAAGGGAGATCCTACACCTACTGGAATTGGA
TGTTCTAGGGGGGTGTGAAT

Downstream 100 bases:

>100_bases
TTAGACTTCCGCTCCTCCCAACCGTAATTAAAAACCTCTTCAAGAAACCTGCAACTAATCCTTTCCCAAAGACTGAACCA
GTTCCAGTTCCTGAGGATTT

Product: mbh13 NADH dehydrogenase subunit

Products: NA

Alternate protein names: FHL subunit 4; Hydrogenase 3 component D [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MKIVYGVIGLILIYIYVSVVSLLFSGIDRKLVARMQRRIGPPILQPFYDFLKLMSKETIIPKTANFMFKAAPILMLATVI
ALLAYTPLGFPPIFGTKGDIIVFIYLLTLADFFLVVGVMSSGSPYGRIGAARGIALLVSREPAMMLGVFAVMWAISKLGV
EKPFSLSSLYEHTIWDFGPVAWVAGVVLIYVFMAWLASEIEVGFFNIPEAEQEIAEGTLVEYSGRYLGIIKLAESIKEFI
AASLVVAVLFPWQLNIPGVQGYLINLLLHTLKVFIVLLVSKTIFRTITGRLKISQAVNLLWTRVFTASVIGALLLALGVM
L

Sequences:

>Translated_321_residues
MKIVYGVIGLILIYIYVSVVSLLFSGIDRKLVARMQRRIGPPILQPFYDFLKLMSKETIIPKTANFMFKAAPILMLATVI
ALLAYTPLGFPPIFGTKGDIIVFIYLLTLADFFLVVGVMSSGSPYGRIGAARGIALLVSREPAMMLGVFAVMWAISKLGV
EKPFSLSSLYEHTIWDFGPVAWVAGVVLIYVFMAWLASEIEVGFFNIPEAEQEIAEGTLVEYSGRYLGIIKLAESIKEFI
AASLVVAVLFPWQLNIPGVQGYLINLLLHTLKVFIVLLVSKTIFRTITGRLKISQAVNLLWTRVFTASVIGALLLALGVM
L
>Mature_321_residues
MKIVYGVIGLILIYIYVSVVSLLFSGIDRKLVARMQRRIGPPILQPFYDFLKLMSKETIIPKTANFMFKAAPILMLATVI
ALLAYTPLGFPPIFGTKGDIIVFIYLLTLADFFLVVGVMSSGSPYGRIGAARGIALLVSREPAMMLGVFAVMWAISKLGV
EKPFSLSSLYEHTIWDFGPVAWVAGVVLIYVFMAWLASEIEVGFFNIPEAEQEIAEGTLVEYSGRYLGIIKLAESIKEFI
AASLVVAVLFPWQLNIPGVQGYLINLLLHTLKVFIVLLVSKTIFRTITGRLKISQAVNLLWTRVFTASVIGALLLALGVM
L

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG0650

COG function: function code C; Formate hydrogenlyase subunit 4

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789077, Length=307, Percent_Identity=29.6416938110749, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI87082115, Length=234, Percent_Identity=32.0512820512821, Blast_Score=111, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001694
- InterPro:   IPR018086 [H]

Pfam domain/function: PF00146 NADHdh [H]

EC number: NA

Molecular weight: Translated: 35388; Mature: 35388

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVYGVIGLILIYIYVSVVSLLFSGIDRKLVARMQRRIGPPILQPFYDFLKLMSKETII
CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
PKTANFMFKAAPILMLATVIALLAYTPLGFPPIFGTKGDIIVFIYLLTLADFFLVVGVMS
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
SGSPYGRIGAARGIALLVSREPAMMLGVFAVMWAISKLGVEKPFSLSSLYEHTIWDFGPV
CCCCCCHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHH
AWVAGVVLIYVFMAWLASEIEVGFFNIPEAEQEIAEGTLVEYSGRYLGIIKLAESIKEFI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHH
AASLVVAVLFPWQLNIPGVQGYLINLLLHTLKVFIVLLVSKTIFRTITGRLKISQAVNLL
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WTRVFTASVIGALLLALGVML
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIVYGVIGLILIYIYVSVVSLLFSGIDRKLVARMQRRIGPPILQPFYDFLKLMSKETII
CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
PKTANFMFKAAPILMLATVIALLAYTPLGFPPIFGTKGDIIVFIYLLTLADFFLVVGVMS
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
SGSPYGRIGAARGIALLVSREPAMMLGVFAVMWAISKLGVEKPFSLSSLYEHTIWDFGPV
CCCCCCHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHH
AWVAGVVLIYVFMAWLASEIEVGFFNIPEAEQEIAEGTLVEYSGRYLGIIKLAESIKEFI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHH
AASLVVAVLFPWQLNIPGVQGYLINLLLHTLKVFIVLLVSKTIFRTITGRLKISQAVNLL
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WTRVFTASVIGALLLALGVML
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]