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The map label for this gene is thrS [H]

Identifier: 18977723

GI number: 18977723

Start: 1269025

End: 1270902

Strand: Reverse

Name: thrS [H]

Synonym: PF1351

Alternate gene names: 18977723

Gene position: 1270902-1269025 (Counterclockwise)

Preceding gene: 18977724

Following gene: 18977720

Centisome position: 66.6

GC content: 47.23

Gene sequence:

>1878_bases
ATGAGAATGCTTCTAATTCACAGCGACTATATCGAGTACGAAGTCAAGGACAAGGCCATTAAAAACCCAGAACCGATAAG
TGAGGAGGAAAAGAAAGGAAGAATGGACGAAGTTCTCGTCGCATTCATAAGCGTCGAGAAAGTTGATGAAAAGAATCCAG
ATGAGGTTGTTGAGAAGGCAATCAACGAGATAATAGAGGTTGCAAAGCAGGTAAAAGCTGAGAACCTCTTCGTTTATCCC
TTCGCCCACTTAAGCAGTGAGCTTGCAAAGCCCTCAGTTGCTCAGGAAGTCCTTAGAAGAATATACGAAGGACTTAAAGA
GAGGGGTTACAACGTTGGAAAGGCACCGTTCGGCTACTATAAGGCCTTCAGGATAAGTTGTAAGGGACACCCATTGGCAG
AGCTGAGCAGAACAATAGTCCCAGAAGAGGCAAAGGTGGAGGAAGTTCCAGAGGCCCTCAAGAAAGAAGAAACCGAGTTG
GTAAGCTATTGGTACATCTTAACCCCCGAGGGAGAGTTAGTGGAGGTTGATAAATTCGACTTCACGGGCCATGAAAACCT
AAGGAAGTTCGCCAACTATGAGATAGCTAAGAGTAGAATTGCCGATAAGGAACCTCCGCACGTTAGGTTAATGCTCGAGC
ATGAGCTCGTTGATTATGAACCTGGGAGTGACCCAGGAAACCTTCGCTATTATCCAAAGGGTAGGCTAATCAAATCGCTC
CTCGAGCAGTACGTAAGTGAGAAGGTCATCGAGTACGGGGCAATGGAGGTTGAGACCCCGATAATGTACGACTTCGAGCA
CCCAGCATTGGAGAAGTACTTAAACAGATTCCCTGCAAGGCAGTACATAGTGCTGAGCGGGGATAAGAGGTACTTCTTGA
GGTTTGCAGCTTGCTTTGGCCAGTTCATGATAAGTAAGGATGCAACTATAAGCTATAGGAACTTGCCGCTGAGGATGTAC
GAGCTCACCCGCTATTCATTCAGGAGAGAGAAGAGGGGGGAGCTCTCAGGTTTGAGGAGGCTCAGAGCATTTACAATGCC
GGATATGCATACATTAGCCAAAGACATAGAGCAGGCGAAGGATGAGTTCAAGAAGCAGTTCAAGCTGAGCATGGAGGTAC
TTGGGGGTGTTGGGTTAACCCCAGATGACTATGAAGTGGCCATAAGATTTACGGAGGACTTCTGGAACGAGCACAAGGAC
TTCATAATTGAATTGGTAAAGCTAATAGGAAAGCCCGTGCTCATTGAGATGTGGAAGCAGAGGTTCTTCTACTTTATACT
CAAGTTCGAGTTCAACTTCGTCGACAACCTTGACAAAGCGGCAGCATTAAGCACAGTTCAAATTGACGTTGAGAACGCCG
AGAGGTTTGGAATTACGTACTACGATGAGAACGGAGAAGAGAAGTACCCACTGATCCTCCATTGTTCGCCTAGCGGTGCA
ATTGAAAGGGTTATGTACGCTATTCTCGAAAAGCAGGCTAAGCTGATGCAGGAAGGAAAGAAGCCAATGTTCCCGCTCTG
GCTCAGCCCAATTCAAGTAAGGGTAATCCCGGTGAGCAAAGAATACTTGGACTATGCACTGTACGTTGCTGGAAAGATAG
AAGGGGCAAGAATAAGGGTTGATGTGGACGATGAAGACGAAAGGCTGAGCAAGAAGATCAGGAGGGCGGAGAAGGAGTGG
ATACCCTACATAGTGGTTGTGGGAGAGAAGGAGAAAGAGACTGGAACGATAACAGTAAGGAGGAGAGAGGACGGCAAACA
ATACGAGACAAGGATTGAGGAGCTCATAAAGGAGATAAAGCAGAAGACGGAGGGCTTCCCCTACAAGCCAAGGCCCTTAC
CTCTCTTACTCAGCCAGAGGCCAAAGTTCAGGGGCTGA

Upstream 100 bases:

>100_bases
TTGGAATTAAAGTGGAATACGTGGAGTAATAGTAAGATCTCCAAACCCAAATGCAAAGATATTTAAAAATCCACGCTTTT
AGGTTAAAGGTGGTGATAAT

Downstream 100 bases:

>100_bases
CCTCTTCCCATCATTATTGGAACCATTGAAAGTCCAGTTAGGGTTAAGCCGAGCACGAAGTCCGTGGTGGCACTGAAGGA
GTTAACTATGTATCCGCTAG

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 625; Mature: 625

Protein sequence:

>625_residues
MRMLLIHSDYIEYEVKDKAIKNPEPISEEEKKGRMDEVLVAFISVEKVDEKNPDEVVEKAINEIIEVAKQVKAENLFVYP
FAHLSSELAKPSVAQEVLRRIYEGLKERGYNVGKAPFGYYKAFRISCKGHPLAELSRTIVPEEAKVEEVPEALKKEETEL
VSYWYILTPEGELVEVDKFDFTGHENLRKFANYEIAKSRIADKEPPHVRLMLEHELVDYEPGSDPGNLRYYPKGRLIKSL
LEQYVSEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIVLSGDKRYFLRFAACFGQFMISKDATISYRNLPLRMY
ELTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAKDEFKKQFKLSMEVLGGVGLTPDDYEVAIRFTEDFWNEHKD
FIIELVKLIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGITYYDENGEEKYPLILHCSPSGA
IERVMYAILEKQAKLMQEGKKPMFPLWLSPIQVRVIPVSKEYLDYALYVAGKIEGARIRVDVDDEDERLSKKIRRAEKEW
IPYIVVVGEKEKETGTITVRRREDGKQYETRIEELIKEIKQKTEGFPYKPRPLPLLLSQRPKFRG

Sequences:

>Translated_625_residues
MRMLLIHSDYIEYEVKDKAIKNPEPISEEEKKGRMDEVLVAFISVEKVDEKNPDEVVEKAINEIIEVAKQVKAENLFVYP
FAHLSSELAKPSVAQEVLRRIYEGLKERGYNVGKAPFGYYKAFRISCKGHPLAELSRTIVPEEAKVEEVPEALKKEETEL
VSYWYILTPEGELVEVDKFDFTGHENLRKFANYEIAKSRIADKEPPHVRLMLEHELVDYEPGSDPGNLRYYPKGRLIKSL
LEQYVSEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIVLSGDKRYFLRFAACFGQFMISKDATISYRNLPLRMY
ELTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAKDEFKKQFKLSMEVLGGVGLTPDDYEVAIRFTEDFWNEHKD
FIIELVKLIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGITYYDENGEEKYPLILHCSPSGA
IERVMYAILEKQAKLMQEGKKPMFPLWLSPIQVRVIPVSKEYLDYALYVAGKIEGARIRVDVDDEDERLSKKIRRAEKEW
IPYIVVVGEKEKETGTITVRRREDGKQYETRIEELIKEIKQKTEGFPYKPRPLPLLLSQRPKFRG
>Mature_625_residues
MRMLLIHSDYIEYEVKDKAIKNPEPISEEEKKGRMDEVLVAFISVEKVDEKNPDEVVEKAINEIIEVAKQVKAENLFVYP
FAHLSSELAKPSVAQEVLRRIYEGLKERGYNVGKAPFGYYKAFRISCKGHPLAELSRTIVPEEAKVEEVPEALKKEETEL
VSYWYILTPEGELVEVDKFDFTGHENLRKFANYEIAKSRIADKEPPHVRLMLEHELVDYEPGSDPGNLRYYPKGRLIKSL
LEQYVSEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIVLSGDKRYFLRFAACFGQFMISKDATISYRNLPLRMY
ELTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAKDEFKKQFKLSMEVLGGVGLTPDDYEVAIRFTEDFWNEHKD
FIIELVKLIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGITYYDENGEEKYPLILHCSPSGA
IERVMYAILEKQAKLMQEGKKPMFPLWLSPIQVRVIPVSKEYLDYALYVAGKIEGARIRVDVDDEDERLSKKIRRAEKEW
IPYIVVVGEKEKETGTITVRRREDGKQYETRIEELIKEIKQKTEGFPYKPRPLPLLLSQRPKFRG

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38570111, Length=412, Percent_Identity=27.4271844660194, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI38202255, Length=420, Percent_Identity=27.6190476190476, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI20070344, Length=421, Percent_Identity=25.41567695962, Blast_Score=117, Evalue=3e-26,
Organism=Escherichia coli, GI1788013, Length=383, Percent_Identity=27.6762402088773, Blast_Score=153, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI71984184, Length=406, Percent_Identity=25.3694581280788, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71984192, Length=406, Percent_Identity=25.3694581280788, Blast_Score=134, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6322654, Length=440, Percent_Identity=25, Blast_Score=113, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6322112, Length=410, Percent_Identity=26.0975609756098, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24583839, Length=407, Percent_Identity=28.009828009828, Blast_Score=152, Evalue=9e-37,
Organism=Drosophila melanogaster, GI24583842, Length=407, Percent_Identity=28.009828009828, Blast_Score=152, Evalue=9e-37,
Organism=Drosophila melanogaster, GI28574141, Length=407, Percent_Identity=28.009828009828, Blast_Score=152, Evalue=9e-37,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002320
- InterPro:   IPR015011
- ProDom:   PD016189 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF08915 tRNA-Thr_ED [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 73209; Mature: 73209

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMLLIHSDYIEYEVKDKAIKNPEPISEEEKKGRMDEVLVAFISVEKVDEKNPDEVVEKA
CEEEEEECCCEEEEECHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
INEIIEVAKQVKAENLFVYPFAHLSSELAKPSVAQEVLRRIYEGLKERGYNVGKAPFGYY
HHHHHHHHHHHHHCCEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
KAFRISCKGHPLAELSRTIVPEEAKVEEVPEALKKEETELVSYWYILTPEGELVEVDKFD
EEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEEEEEEECCCCCEEEEECCC
FTGHENLRKFANYEIAKSRIADKEPPHVRLMLEHELVDYEPGSDPGNLRYYPKGRLIKSL
CCCHHHHHHHHCHHHHHHHCCCCCCCEEEEEEEECEECCCCCCCCCCEEECCCHHHHHHH
LEQYVSEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIVLSGDKRYFLRFAACFG
HHHHHHHHHHHCCCEEECCCEEECCCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHH
QFMISKDATISYRNLPLRMYELTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAK
HHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
DEFKKQFKLSMEVLGGVGLTPDDYEVAIRFTEDFWNEHKDFIIELVKLIGKPVLIEMWKQ
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEHHHHCCHHHHHHHHHHHHCCHHHHHHHHH
RFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGITYYDENGEEKYPLILHCSPSGA
HHEEEEEEEEECHHCCCHHHHHEEEEEEEECCCCEECEEEECCCCCCCCCEEEEECCCHH
IERVMYAILEKQAKLMQEGKKPMFPLWLSPIQVRVIPVSKEYLDYALYVAGKIEGARIRV
HHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEECCHHHHHEEEEEEEEECCCEEEE
DVDDEDERLSKKIRRAEKEWIPYIVVVGEKEKETGTITVRRREDGKQYETRIEELIKEIK
ECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
QKTEGFPYKPRPLPLLLSQRPKFRG
HHHCCCCCCCCCCCHHCCCCCCCCC
>Mature Secondary Structure
MRMLLIHSDYIEYEVKDKAIKNPEPISEEEKKGRMDEVLVAFISVEKVDEKNPDEVVEKA
CEEEEEECCCEEEEECHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
INEIIEVAKQVKAENLFVYPFAHLSSELAKPSVAQEVLRRIYEGLKERGYNVGKAPFGYY
HHHHHHHHHHHHHCCEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
KAFRISCKGHPLAELSRTIVPEEAKVEEVPEALKKEETELVSYWYILTPEGELVEVDKFD
EEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEEEEEEECCCCCEEEEECCC
FTGHENLRKFANYEIAKSRIADKEPPHVRLMLEHELVDYEPGSDPGNLRYYPKGRLIKSL
CCCHHHHHHHHCHHHHHHHCCCCCCCEEEEEEEECEECCCCCCCCCCEEECCCHHHHHHH
LEQYVSEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIVLSGDKRYFLRFAACFG
HHHHHHHHHHHCCCEEECCCEEECCCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHH
QFMISKDATISYRNLPLRMYELTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAK
HHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
DEFKKQFKLSMEVLGGVGLTPDDYEVAIRFTEDFWNEHKDFIIELVKLIGKPVLIEMWKQ
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEHHHHCCHHHHHHHHHHHHCCHHHHHHHHH
RFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGITYYDENGEEKYPLILHCSPSGA
HHEEEEEEEEECHHCCCHHHHHEEEEEEEECCCCEECEEEECCCCCCCCCEEEEECCCHH
IERVMYAILEKQAKLMQEGKKPMFPLWLSPIQVRVIPVSKEYLDYALYVAGKIEGARIRV
HHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEECCHHHHHEEEEEEEEECCCEEEE
DVDDEDERLSKKIRRAEKEWIPYIVVVGEKEKETGTITVRRREDGKQYETRIEELIKEIK
ECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
QKTEGFPYKPRPLPLLLSQRPKFRG
HHHCCCCCCCCCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]