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The map label for this gene is thiM

Identifier: 18977707

GI number: 18977707

Start: 1255811

End: 1256608

Strand: Reverse

Name: thiM

Synonym: PF1335

Alternate gene names: 18977707

Gene position: 1256608-1255811 (Counterclockwise)

Preceding gene: 18977708

Following gene: 18977706

Centisome position: 65.85

GC content: 45.86

Gene sequence:

>798_bases
ATGATTAGCGTCGGGGAGGCCTTAGCTAAGGTTAGGGAAAGAAAGCCCCTAGTCCACAACATTACAAACTTCGTAGTTAT
GAACACCACAGCGAACGCTCTCCTTGCCCTTGGAGCATCTCCAGTCATGGCACATGCAGAAGAAGAGCTCGAAGAGATGG
TAAGGATTGCTGATTCAGTAGTCGTGAACATCGGAACTCTCGACAAATTCTGGATAAATTCTATGATCAAAGCAGCAAAA
CTAGCCAAAGAATATGGGAAACCCCTTGTACTAGATCCAGTAGGAGCCGGTGCAACGAGACTTAGGACGAAGACAGCTCT
AGAGGTACTAAAAATAGGAGCTACCATAGTCAAGGGGAACTTTGGAGAGATATCCGCTCTGCTAGGAGAGGAAGGAAAAA
CTAGGGGAGTGGACTCCTCCACATATGACCCAGAGAGAGCAAAAGAATTGGCATTGGCTGTAGCTGAGGAATTTTCCACA
ATTGTTGCCGTTACTGGTAAAGTAGACTATGTAAGCGATGGTAAAAGAGTTTATGCAGTCTATAATGGCCACGAGCTTCT
TGGAAGGGTTACGGGAACAGGATGTATAGTAGCGGCACTTACTGGAGCCTTCGTTGCAGTAAATGATCCGCTAACTTCAG
CTGTCTCATCCTTGGTTGTCTTTGAAGTAGCAGCTGAAAAAGCAGCTGAAGAGGCAAAAGCCCCTGGAACTTTCCATGCT
AAGCTCTACGACTGGCTCTACTTGCTAACCCCAGAGGATGTAGAAAGGCTAAAGAAAATTGAGGTGGTAAAAGTATGA

Upstream 100 bases:

>100_bases
AAGTACCAGTCTTAAGCGATTTAGGCAACTCAATAGGAGCAAGCATTCCAGTCTTTTTGATCTCTGCCATAATATATTAC
CTCCTTGAGAGGTGGGGAGA

Downstream 100 bases:

>100_bases
ACCTAAGGAACAAGCTGAAGCTCTACGTTATAACAGACAGAAGACTCAAGCCCGAAGTTGAGAGTGTAAGGGAAGCCCTG
GAAGGAGGGGCTACTGCAAT

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MISVGEALAKVRERKPLVHNITNFVVMNTTANALLALGASPVMAHAEEELEEMVRIADSVVVNIGTLDKFWINSMIKAAK
LAKEYGKPLVLDPVGAGATRLRTKTALEVLKIGATIVKGNFGEISALLGEEGKTRGVDSSTYDPERAKELALAVAEEFST
IVAVTGKVDYVSDGKRVYAVYNGHELLGRVTGTGCIVAALTGAFVAVNDPLTSAVSSLVVFEVAAEKAAEEAKAPGTFHA
KLYDWLYLLTPEDVERLKKIEVVKV

Sequences:

>Translated_265_residues
MISVGEALAKVRERKPLVHNITNFVVMNTTANALLALGASPVMAHAEEELEEMVRIADSVVVNIGTLDKFWINSMIKAAK
LAKEYGKPLVLDPVGAGATRLRTKTALEVLKIGATIVKGNFGEISALLGEEGKTRGVDSSTYDPERAKELALAVAEEFST
IVAVTGKVDYVSDGKRVYAVYNGHELLGRVTGTGCIVAALTGAFVAVNDPLTSAVSSLVVFEVAAEKAAEEAKAPGTFHA
KLYDWLYLLTPEDVERLKKIEVVKV
>Mature_265_residues
MISVGEALAKVRERKPLVHNITNFVVMNTTANALLALGASPVMAHAEEELEEMVRIADSVVVNIGTLDKFWINSMIKAAK
LAKEYGKPLVLDPVGAGATRLRTKTALEVLKIGATIVKGNFGEISALLGEEGKTRGVDSSTYDPERAKELALAVAEEFST
IVAVTGKVDYVSDGKRVYAVYNGHELLGRVTGTGCIVAALTGAFVAVNDPLTSAVSSLVVFEVAAEKAAEEAKAPGTFHA
KLYDWLYLLTPEDVERLKKIEVVKV

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=248, Percent_Identity=38.3064516129032, Blast_Score=152, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6325042, Length=284, Percent_Identity=30.6338028169014, Blast_Score=112, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_PYRFU (Q8U191)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_579064.1
- ProteinModelPortal:   Q8U191
- SMR:   Q8U191
- EnsemblBacteria:   EBPYRT00000004061
- GeneID:   1469210
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1335
- NMPDR:   fig|186497.1.peg.1381
- GeneTree:   EBGT00050000022541
- HOGENOM:   HBG351126
- OMA:   AIRGNAG
- ProtClustDB:   PRK09355
- BRENDA:   2.7.1.50
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099
- TIGRFAMs:   TIGR00694

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 28327; Mature: 28327

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00059 ADH_ZINC

Important sites: BINDING 43-43 BINDING 118-118 BINDING 165-165 BINDING 192-192

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISVGEALAKVRERKPLVHNITNFVVMNTTANALLALGASPVMAHAEEELEEMVRIADSV
CCCHHHHHHHHHHCCCHHHHHHHEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
VVNIGTLDKFWINSMIKAAKLAKEYGKPLVLDPVGAGATRLRTKTALEVLKIGATIVKGN
EEECCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHCCCEEECC
FGEISALLGEEGKTRGVDSSTYDPERAKELALAVAEEFSTIVAVTGKVDYVSDGKRVYAV
CHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEECCEEEECCCCEEEEE
YNGHELLGRVTGTGCIVAALTGAFVAVNDPLTSAVSSLVVFEVAAEKAAEEAKAPGTFHA
ECCHHHHHHHCCCHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
KLYDWLYLLTPEDVERLKKIEVVKV
HHHHEEEEECHHHHHHHHHHHEECC
>Mature Secondary Structure
MISVGEALAKVRERKPLVHNITNFVVMNTTANALLALGASPVMAHAEEELEEMVRIADSV
CCCHHHHHHHHHHCCCHHHHHHHEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
VVNIGTLDKFWINSMIKAAKLAKEYGKPLVLDPVGAGATRLRTKTALEVLKIGATIVKGN
EEECCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHCCCEEECC
FGEISALLGEEGKTRGVDSSTYDPERAKELALAVAEEFSTIVAVTGKVDYVSDGKRVYAV
CHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEECCEEEECCCCEEEEE
YNGHELLGRVTGTGCIVAALTGAFVAVNDPLTSAVSSLVVFEVAAEKAAEEAKAPGTFHA
ECCHHHHHHHCCCHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
KLYDWLYLLTPEDVERLKKIEVVKV
HHHHEEEEECHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA