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The map label for this gene is asrB [H]

Identifier: 18977702

GI number: 18977702

Start: 1251022

End: 1251888

Strand: Direct

Name: asrB [H]

Synonym: PF1330

Alternate gene names: 18977702

Gene position: 1251022-1251888 (Clockwise)

Preceding gene: 18977701

Following gene: 18977703

Centisome position: 65.56

GC content: 43.48

Gene sequence:

>867_bases
ATGAACCCCTACAGGAGTTATGATGCAAGAATTATTGAGGTGAAGGAACTCACCTCTCGGGAAAAGTTGTTCTCGTTAAA
ATTCCTTGACAACGAAATTGAGGAAAACTTTACATTCAAACCTGGGCAGTTCGTAATAGTTGACATAAGGGGGTTTGGAG
AATTTCCCATAAGCCTCTGTTCCTCTCCAACAAGAAGGCCAATACAGCTTTGCATCAGAAGAGTGGGGAGAATGACAAAG
TTCATACACAAAATGAACGAGGGAGATATTATCGGAATAAGGGGGCCGTATGGAAATGGATTTCCCATGGATCTCATGGA
GGGATCAAACTTAATCCTAATAGCGGGTGGACTTGGAATGGCTCCGTTAAGGAGCGTTCTTTGGTATGCAATAGACAGCG
GAAAGTATGAGAAGATATACCTCTTCTACGGTACAAAAAGCTACGAGGATATCCTCTTTAGGGATGAAATAATTCACCTC
CTAAAGCATGGGGAAAAGTTGAACTGCCACGTAAAGCTTGCTTACGAAGTAGAGACTCCTTCTTGTATCTACTTAGAAAG
GGGCTTTTCGGAGAAGGTGTGCAAAGGAGTCGTAACTGACCTCTTCAGGGGAGAGGAGTTCGACGTTGAAAACTCTTACG
CTTTAATCTGTGGCCCTCCTGTTATGTACAAGTATGTGATAAGGGAACTGCTAGATAGGGGATTGTCTCCAGGGAGAATA
TACATGACACTGGAGAGAAGAATGAGGTGTGGAGTTGGAAAGTGTGGTCACTGCATAGTTGGAACGAGTGTATCCATTAA
GTACATCTGTAAGGATGGTCCCGTTTTCACTTATTGGGATGCTCTTTCCACAAGGGGGTTGATATGA

Upstream 100 bases:

>100_bases
AGGCAGGCTTCAATTGCGTTGGCTGTGGAAGGTGTGATGAGTTCTGTCCGGCTAGAATAGAGCACGTTAAGGTTCTCGAC
GAGGTTAGGGAGGGGTTGAT

Downstream 100 bases:

>100_bases
TGAAGTTGGGTGTTTTTGAACTTACGGACTGTGGTGGATGTGCGCTCAACCTACTATTCCTCTATGATAAGCTTCTCGAC
CTCCTCGAGTTCTATGAAAT

Product: cytochrome-c3 hydrogenase subunit gamma

Products: @TETR01txt*tetrahydropteridine!; NO3-; NAD(P); Proton [C]

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MNPYRSYDARIIEVKELTSREKLFSLKFLDNEIEENFTFKPGQFVIVDIRGFGEFPISLCSSPTRRPIQLCIRRVGRMTK
FIHKMNEGDIIGIRGPYGNGFPMDLMEGSNLILIAGGLGMAPLRSVLWYAIDSGKYEKIYLFYGTKSYEDILFRDEIIHL
LKHGEKLNCHVKLAYEVETPSCIYLERGFSEKVCKGVVTDLFRGEEFDVENSYALICGPPVMYKYVIRELLDRGLSPGRI
YMTLERRMRCGVGKCGHCIVGTSVSIKYICKDGPVFTYWDALSTRGLI

Sequences:

>Translated_288_residues
MNPYRSYDARIIEVKELTSREKLFSLKFLDNEIEENFTFKPGQFVIVDIRGFGEFPISLCSSPTRRPIQLCIRRVGRMTK
FIHKMNEGDIIGIRGPYGNGFPMDLMEGSNLILIAGGLGMAPLRSVLWYAIDSGKYEKIYLFYGTKSYEDILFRDEIIHL
LKHGEKLNCHVKLAYEVETPSCIYLERGFSEKVCKGVVTDLFRGEEFDVENSYALICGPPVMYKYVIRELLDRGLSPGRI
YMTLERRMRCGVGKCGHCIVGTSVSIKYICKDGPVFTYWDALSTRGLI
>Mature_288_residues
MNPYRSYDARIIEVKELTSREKLFSLKFLDNEIEENFTFKPGQFVIVDIRGFGEFPISLCSSPTRRPIQLCIRRVGRMTK
FIHKMNEGDIIGIRGPYGNGFPMDLMEGSNLILIAGGLGMAPLRSVLWYAIDSGKYEKIYLFYGTKSYEDILFRDEIIHL
LKHGEKLNCHVKLAYEVETPSCIYLERGFSEKVCKGVVTDLFRGEEFDVENSYALICGPPVMYKYVIRELLDRGLSPGRI
YMTLERRMRCGVGKCGHCIVGTSVSIKYICKDGPVFTYWDALSTRGLI

Specific function: This enzyme catalyzes the hydrogen sulfide production from sulfite. It is strictly anaerobic. It is regulated by electron acceptors rather than by cysteine [H]

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012165
- InterPro:   IPR019480
- InterPro:   IPR017927
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938
- InterPro:   IPR014260 [H]

Pfam domain/function: PF10418 DHODB_Fe-S_bind; PF00970 FAD_binding_6; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.34 [C]

Molecular weight: Translated: 32946; Mature: 32946

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPYRSYDARIIEVKELTSREKLFSLKFLDNEIEENFTFKPGQFVIVDIRGFGEFPISLC
CCCCCCCCCEEEEEHHHCCHHHEEEEEHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHC
SSPTRRPIQLCIRRVGRMTKFIHKMNEGDIIGIRGPYGNGFPMDLMEGSNLILIAGGLGM
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHCCCCCEEEEECCCCH
APLRSVLWYAIDSGKYEKIYLFYGTKSYEDILFRDEIIHLLKHGEKLNCHVKLAYEVETP
HHHHHHHHEEECCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECCC
SCIYLERGFSEKVCKGVVTDLFRGEEFDVENSYALICGPPVMYKYVIRELLDRGLSPGRI
CEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCEE
YMTLERRMRCGVGKCGHCIVGTSVSIKYICKDGPVFTYWDALSTRGLI
EEEEEHHHHCCCCCCCCEEEECCEEEEEEECCCCEEEEEHHHHCCCCC
>Mature Secondary Structure
MNPYRSYDARIIEVKELTSREKLFSLKFLDNEIEENFTFKPGQFVIVDIRGFGEFPISLC
CCCCCCCCCEEEEEHHHCCHHHEEEEEHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHC
SSPTRRPIQLCIRRVGRMTKFIHKMNEGDIIGIRGPYGNGFPMDLMEGSNLILIAGGLGM
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHCCCCCEEEEECCCCH
APLRSVLWYAIDSGKYEKIYLFYGTKSYEDILFRDEIIHLLKHGEKLNCHVKLAYEVETP
HHHHHHHHEEECCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECCC
SCIYLERGFSEKVCKGVVTDLFRGEEFDVENSYALICGPPVMYKYVIRELLDRGLSPGRI
CEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCEE
YMTLERRMRCGVGKCGHCIVGTSVSIKYICKDGPVFTYWDALSTRGLI
EEEEEHHHHCCCCCCCCEEEECCEEEEEEECCCCEEEEEHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dihydropteridine; 2 nitric oxide; O2; NAD(P)H [C]

Specific reaction: NAD(P)H + dihydropteridine = NAD(P)+ @TETR01.txt*tetrahydropteridine! 2 nitric oxide + 2 O2 + NAD(P)H = 2 NO3- + NAD(P) + Proton [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1704886; 11677609 [H]