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The map label for this gene is appB [H]

Identifier: 18977582

GI number: 18977582

Start: 1151648

End: 1152634

Strand: Direct

Name: appB [H]

Synonym: PF1210

Alternate gene names: 18977582

Gene position: 1151648-1152634 (Clockwise)

Preceding gene: 18977581

Following gene: 18977583

Centisome position: 60.35

GC content: 42.76

Gene sequence:

>987_bases
ATGATGAACTACCTCAAGAGGAAGATAGTGATATACATTTTAACATTCATATTTGCCGTCACAATAGACTTTATAATTCC
CAGGCTGATGCCTGGGGATCCAATAGCTGTGTTATTATCTAGATTCGCTACAATGCCCCAAGCAACTCAATACCTTCATG
GGTATTTTATTGAGGCATTTGGATTAAATAAGCCTTTATGGGAACAATACATAACATTTTGGAAGGCCGTCCTTCACGGT
GATTTAGGAATTAGTATATATTTCTACCCAAAACCCGTTGCTCAAATTATAAAGGAAGCTTTACCTTATGATTTAGCCTT
ACTTGTCCCCTCAATAGTTGCGAGCTGGATTGTTGGTAATTGGTTGGGAGCTTACGCTGGTAAAAATAGGAAGCTCGACT
CTTATTTAATGCCCGTATTCTTCTTCCTCCAAGCATCACCTTACTTCTGGTTTGCAATCCTCTTAGCCTATATATTCTCA
GTTAAGTTAGAATTGTTCCCGCTCTCAGGTGCCTACAGTTATGGAATAATCCCCTCACTCAGCTGGGCATTCATAAAGGA
CTACCTACACCACTGGGTGCTGCCATTCCTCAGCCTATTCCTAGTCCAACTTGGTGGATGGGCCATAGGTATGAGAAACA
TGATAATCTATGAGCTCGAGGCAGACTATGTGAGGTACCTAGAAGCTCTAGGATCGAGCGAAAGAACAGTCATGAGGCAT
GCATTCAAAAACGCAATGCTACCCCAGATAACAGGATTAGCACTCCAGCTCGGACTGATAGTTGCCGGTAACGTCACCGT
TCAAGTTGTTTTCTCATACCCAGGAATAGGGTATATCCTCATGCAAGGAATACTCAACCAGGACTACTTCCTAATCCAGG
GCTGCTTCCTTGTTATAATCCTAACGGTCCTAGCTGCCAACTTCATAATAGACATAATGTACGCCCTAATCGATCCAAGA
ATAAAGGCCGCCTACGTGGAGGGATGA

Upstream 100 bases:

>100_bases
CCACTCCTCCTAAGGAGGAGAAGATCTTAACCTTCTAATTTATTTATTTTTTTGCAGAATTTTAAGAAAAAGTCAAGGAG
GGAGAAAATTGGCCAGCGGA

Downstream 100 bases:

>100_bases
AGAAATGCCAGTTGGAAAATCAAAGTTTGAAGTCCTCAAAATCGCGCTAAAAAACATAAAGTTTAAAGTTGGGCTTGGAA
TCGTCATATTCTTTGCCCTA

Product: dipeptide ABC transporter, permease protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MMNYLKRKIVIYILTFIFAVTIDFIIPRLMPGDPIAVLLSRFATMPQATQYLHGYFIEAFGLNKPLWEQYITFWKAVLHG
DLGISIYFYPKPVAQIIKEALPYDLALLVPSIVASWIVGNWLGAYAGKNRKLDSYLMPVFFFLQASPYFWFAILLAYIFS
VKLELFPLSGAYSYGIIPSLSWAFIKDYLHHWVLPFLSLFLVQLGGWAIGMRNMIIYELEADYVRYLEALGSSERTVMRH
AFKNAMLPQITGLALQLGLIVAGNVTVQVVFSYPGIGYILMQGILNQDYFLIQGCFLVIILTVLAANFIIDIMYALIDPR
IKAAYVEG

Sequences:

>Translated_328_residues
MMNYLKRKIVIYILTFIFAVTIDFIIPRLMPGDPIAVLLSRFATMPQATQYLHGYFIEAFGLNKPLWEQYITFWKAVLHG
DLGISIYFYPKPVAQIIKEALPYDLALLVPSIVASWIVGNWLGAYAGKNRKLDSYLMPVFFFLQASPYFWFAILLAYIFS
VKLELFPLSGAYSYGIIPSLSWAFIKDYLHHWVLPFLSLFLVQLGGWAIGMRNMIIYELEADYVRYLEALGSSERTVMRH
AFKNAMLPQITGLALQLGLIVAGNVTVQVVFSYPGIGYILMQGILNQDYFLIQGCFLVIILTVLAANFIIDIMYALIDPR
IKAAYVEG
>Mature_328_residues
MMNYLKRKIVIYILTFIFAVTIDFIIPRLMPGDPIAVLLSRFATMPQATQYLHGYFIEAFGLNKPLWEQYITFWKAVLHG
DLGISIYFYPKPVAQIIKEALPYDLALLVPSIVASWIVGNWLGAYAGKNRKLDSYLMPVFFFLQASPYFWFAILLAYIFS
VKLELFPLSGAYSYGIIPSLSWAFIKDYLHHWVLPFLSLFLVQLGGWAIGMRNMIIYELEADYVRYLEALGSSERTVMRH
AFKNAMLPQITGLALQLGLIVAGNVTVQVVFSYPGIGYILMQGILNQDYFLIQGCFLVIILTVLAANFIIDIMYALIDPR
IKAAYVEG

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789965, Length=345, Percent_Identity=28.695652173913, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI1787053, Length=325, Percent_Identity=29.5384615384615, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1789888, Length=326, Percent_Identity=30.9815950920245, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1787497, Length=326, Percent_Identity=27.9141104294479, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1788504, Length=362, Percent_Identity=27.9005524861878, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1787761, Length=342, Percent_Identity=27.7777777777778, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1787550, Length=273, Percent_Identity=29.3040293040293, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 37343; Mature: 37343

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNYLKRKIVIYILTFIFAVTIDFIIPRLMPGDPIAVLLSRFATMPQATQYLHGYFIEAF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHH
GLNKPLWEQYITFWKAVLHGDLGISIYFYPKPVAQIIKEALPYDLALLVPSIVASWIVGN
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
WLGAYAGKNRKLDSYLMPVFFFLQASPYFWFAILLAYIFSVKLELFPLSGAYSYGIIPSL
HHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCH
SWAFIKDYLHHWVLPFLSLFLVQLGGWAIGMRNMIIYELEADYVRYLEALGSSERTVMRH
HHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCEEEEEECHHHHHHHHHHCCCHHHHHHH
AFKNAMLPQITGLALQLGLIVAGNVTVQVVFSYPGIGYILMQGILNQDYFLIQGCFLVII
HHHHCCCHHHHHHHHHHHHHEECCEEEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHH
LTVLAANFIIDIMYALIDPRIKAAYVEG
HHHHHHHHHHHHHHHHHCCCHHEEECCC
>Mature Secondary Structure
MMNYLKRKIVIYILTFIFAVTIDFIIPRLMPGDPIAVLLSRFATMPQATQYLHGYFIEAF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHH
GLNKPLWEQYITFWKAVLHGDLGISIYFYPKPVAQIIKEALPYDLALLVPSIVASWIVGN
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
WLGAYAGKNRKLDSYLMPVFFFLQASPYFWFAILLAYIFSVKLELFPLSGAYSYGIIPSL
HHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCH
SWAFIKDYLHHWVLPFLSLFLVQLGGWAIGMRNMIIYELEADYVRYLEALGSSERTVMRH
HHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCEEEEEECHHHHHHHHHHCCCHHHHHHH
AFKNAMLPQITGLALQLGLIVAGNVTVQVVFSYPGIGYILMQGILNQDYFLIQGCFLVII
HHHHCCCHHHHHHHHHHHHHEECCEEEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHH
LTVLAANFIIDIMYALIDPRIKAAYVEG
HHHHHHHHHHHHHHHHHCCCHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]