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The map label for this gene is appA [H]

Identifier: 18977581

GI number: 18977581

Start: 1149709

End: 1151577

Strand: Direct

Name: appA [H]

Synonym: PF1209

Alternate gene names: 18977581

Gene position: 1149709-1151577 (Clockwise)

Preceding gene: 18977577

Following gene: 18977582

Centisome position: 60.25

GC content: 46.12

Gene sequence:

>1869_bases
ATGAAGAGACTCGTTGGTGTACTAATTGGAGCGTTTGTAATATTTGGAGTTTTCGGCCAAGTAGTGGCCGCCCAGGAACA
AGAACTTCCAAGGGAAGAAACACTATACGTTGGTGGTGGTCTCTGGAGCCAACCAAACAACTTCAATCCCCTTATCCCCT
GGAGTGCCGTTACAGGAACCATTGGCTTGATTTACGAAACCTTGTTCAACTACGACCCATTAAACGATAAGCTAGAGCCC
TGGCTTGCCGAGAGTGGAAGATGGGTTAGCGACAACGTATATGAGGTTAAACTTAGGGAAGGATTAACCTGGCAGGATGG
AAAGCCACTAACAGCTGAGGACGTTAAGTTCACCTTCGAGCTCCACAAGAAGTATCCTGGTCTAGCATATCACCAGATGT
GGGAGTGGCTCCAAGAGGTTAAGGTTATAGATGACAGAACAATCCAGTTTGTATTTAGCACTCCCCACTATGAAGAGTGG
AAGTATCTACTTTATCAAATAGCAATTGTTCCGAAGCACATCTGGCAGAACATTGATAACCCAGTTGAATTCGCCAACGT
TGACAACCCAGTTGGTTCTGGTCCCTACAAGCTCTACAAGACAGACCAGATGAGGTTCATTCTCGTTAGGAACGACAACT
GGTGGGGAATCAAGTACTTCGGCAAGCCCGCTCCAAAGTACATAGTCTACGTTATAGTCTACAGCAACAACTTGGCATTA
TCAATGCTCGTCAAGGGTGAGCTCGACTGGAGCAACTTCTTCATCCCGGGTGTCCCAGATGTCAAGGCTCAGTATGGAAT
AGTTACCTGGTACGATGAACCACCATACCACCTACCAGCAAACACGGCATTCCTATTCCTCAACACCCAAAAGCCACCAC
TTGACAACCCAGAGTTCAGAAGGGCCATAGCCTATGCAATAAACCCAGAGGAGATAGCTGCCAGAGCATCCCAAAACCAG
GTAACTCCAGCCGATCCCACAGGATTACTAATCCAGTATCCAGCATTCAAGAAGATATACAACAAGGAGGCCGTTGATAA
GTACGGATTCAAGTATGATCCACAAAAGGCCATGGAGATACTCGACAAGCTAGGATTTAAGGATGTAAATGGTGATGGCT
ACAGAGAGTTCCCAGATGGAAAACCACTCAAGTTAACAATAATAGTCCCATTCGGATGGACCGACTGGATGGAGATGATC
AGAGTAATAAGCGAGCAGTTGGCAATTGTTGGAATCAACGTCGAGCCCAAGTTCCCAGACTATTCAAAGTACTGGGATGA
CCTAACCAAGGGTAACTTCGACATGGCCATAAACAACTTCGGAAGCAACGTAGTTGCAACACCATGGCAATGGTTCAACT
GGGTCCTCAACCAAGATGTCACTCCAATAGGAGAGAACTTCTACAACGGTAACTTCGGTAGATACAAGAATGAGAGAGTT
GCAGAGCTCCTAGAGCTCATAAACGTTGAGAAGGATGAGAACAAGAAGCTTCAATACTACTACGAGCTACAGGAGATATT
CCTCAAAGACTTGCCATACATACCAATAATCTACAACGGTGCATGGTTTGAGGCAAGCCCAGAGCACTGGACCAACTGGC
CTACAGCAAAGAACCCATACGCATTCCCAATAAGCTGGAACGGATACTGGCAGATGGGTGGAGTGAAGGTGCTACTCAAT
ATCAAGCCAGTAAAGGCAGCTACAGAAACAACAACTACAGAGGAGAAGACTGAAACGATAGTTCAGACTGTTACCGTTAC
TCCAACAGAGACGGCAACTTCATCAACAGAAACAGGAGGAATCTGTGGACCAGCAATATTAGTTGGTTTAGCCGTAGTCC
CACTCCTCCTAAGGAGGAGAAGATCTTAA

Upstream 100 bases:

>100_bases
ATTTGGCCCAATGATTATAAATATACAGCAAGATTTATAAGCATTGAGGATTTCATTATAATTGACAATGTGCTTTGTTG
CATCCTTACGGGGTGATAGT

Downstream 100 bases:

>100_bases
CCTTCTAATTTATTTATTTTTTTGCAGAATTTTAAGAAAAAGTCAAGGAGGGAGAAAATTGGCCAGCGGAATGATGAACT
ACCTCAAGAGGAAGATAGTG

Product: oligopeptide ABC transporter

Products: ADP; phosphate; peptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 622; Mature: 622

Protein sequence:

>622_residues
MKRLVGVLIGAFVIFGVFGQVVAAQEQELPREETLYVGGGLWSQPNNFNPLIPWSAVTGTIGLIYETLFNYDPLNDKLEP
WLAESGRWVSDNVYEVKLREGLTWQDGKPLTAEDVKFTFELHKKYPGLAYHQMWEWLQEVKVIDDRTIQFVFSTPHYEEW
KYLLYQIAIVPKHIWQNIDNPVEFANVDNPVGSGPYKLYKTDQMRFILVRNDNWWGIKYFGKPAPKYIVYVIVYSNNLAL
SMLVKGELDWSNFFIPGVPDVKAQYGIVTWYDEPPYHLPANTAFLFLNTQKPPLDNPEFRRAIAYAINPEEIAARASQNQ
VTPADPTGLLIQYPAFKKIYNKEAVDKYGFKYDPQKAMEILDKLGFKDVNGDGYREFPDGKPLKLTIIVPFGWTDWMEMI
RVISEQLAIVGINVEPKFPDYSKYWDDLTKGNFDMAINNFGSNVVATPWQWFNWVLNQDVTPIGENFYNGNFGRYKNERV
AELLELINVEKDENKKLQYYYELQEIFLKDLPYIPIIYNGAWFEASPEHWTNWPTAKNPYAFPISWNGYWQMGGVKVLLN
IKPVKAATETTTTEEKTETIVQTVTVTPTETATSSTETGGICGPAILVGLAVVPLLLRRRRS

Sequences:

>Translated_622_residues
MKRLVGVLIGAFVIFGVFGQVVAAQEQELPREETLYVGGGLWSQPNNFNPLIPWSAVTGTIGLIYETLFNYDPLNDKLEP
WLAESGRWVSDNVYEVKLREGLTWQDGKPLTAEDVKFTFELHKKYPGLAYHQMWEWLQEVKVIDDRTIQFVFSTPHYEEW
KYLLYQIAIVPKHIWQNIDNPVEFANVDNPVGSGPYKLYKTDQMRFILVRNDNWWGIKYFGKPAPKYIVYVIVYSNNLAL
SMLVKGELDWSNFFIPGVPDVKAQYGIVTWYDEPPYHLPANTAFLFLNTQKPPLDNPEFRRAIAYAINPEEIAARASQNQ
VTPADPTGLLIQYPAFKKIYNKEAVDKYGFKYDPQKAMEILDKLGFKDVNGDGYREFPDGKPLKLTIIVPFGWTDWMEMI
RVISEQLAIVGINVEPKFPDYSKYWDDLTKGNFDMAINNFGSNVVATPWQWFNWVLNQDVTPIGENFYNGNFGRYKNERV
AELLELINVEKDENKKLQYYYELQEIFLKDLPYIPIIYNGAWFEASPEHWTNWPTAKNPYAFPISWNGYWQMGGVKVLLN
IKPVKAATETTTTEEKTETIVQTVTVTPTETATSSTETGGICGPAILVGLAVVPLLLRRRRS
>Mature_622_residues
MKRLVGVLIGAFVIFGVFGQVVAAQEQELPREETLYVGGGLWSQPNNFNPLIPWSAVTGTIGLIYETLFNYDPLNDKLEP
WLAESGRWVSDNVYEVKLREGLTWQDGKPLTAEDVKFTFELHKKYPGLAYHQMWEWLQEVKVIDDRTIQFVFSTPHYEEW
KYLLYQIAIVPKHIWQNIDNPVEFANVDNPVGSGPYKLYKTDQMRFILVRNDNWWGIKYFGKPAPKYIVYVIVYSNNLAL
SMLVKGELDWSNFFIPGVPDVKAQYGIVTWYDEPPYHLPANTAFLFLNTQKPPLDNPEFRRAIAYAINPEEIAARASQNQ
VTPADPTGLLIQYPAFKKIYNKEAVDKYGFKYDPQKAMEILDKLGFKDVNGDGYREFPDGKPLKLTIIVPFGWTDWMEMI
RVISEQLAIVGINVEPKFPDYSKYWDDLTKGNFDMAINNFGSNVVATPWQWFNWVLNQDVTPIGENFYNGNFGRYKNERV
AELLELINVEKDENKKLQYYYELQEIFLKDLPYIPIIYNGAWFEASPEHWTNWPTAKNPYAFPISWNGYWQMGGVKVLLN
IKPVKAATETTTTEEKTETIVQTVTVTPTETATSSTETGGICGPAILVGLAVVPLLLRRRRS

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787551, Length=397, Percent_Identity=28.2115869017632, Blast_Score=96, Evalue=1e-20,
Organism=Escherichia coli, GI1787052, Length=489, Percent_Identity=24.9488752556237, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1787762, Length=463, Percent_Identity=23.110151187905, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 71607; Mature: 71607

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLVGVLIGAFVIFGVFGQVVAAQEQELPREETLYVGGGLWSQPNNFNPLIPWSAVTGT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCCCCCCCCCCCHHHHHH
IGLIYETLFNYDPLNDKLEPWLAESGRWVSDNVYEVKLREGLTWQDGKPLTAEDVKFTFE
HHHHHHHHHCCCCCCCCCCHHHHCCCCEECCCEEEEEEECCCCCCCCCCCCHHHEEEEEE
LHKKYPGLAYHQMWEWLQEVKVIDDRTIQFVFSTPHYEEWKYLLYQIAIVPKHIWQNIDN
EHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCC
PVEFANVDNPVGSGPYKLYKTDQMRFILVRNDNWWGIKYFGKPAPKYIVYVIVYSNNLAL
CEEECCCCCCCCCCCEEEEECCCEEEEEEECCCEEEEEECCCCCCCEEEEEEEEECCEEE
SMLVKGELDWSNFFIPGVPDVKAQYGIVTWYDEPPYHLPANTAFLFLNTQKPPLDNPEFR
EEEEECCCCCCCEECCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCCCCHHHH
RAIAYAINPEEIAARASQNQVTPADPTGLLIQYPAFKKIYNKEAVDKYGFKYDPQKAMEI
HHEEEECCHHHHHHHCCCCCCCCCCCCCEEEECCHHHHHHCHHHHHHCCCCCCHHHHHHH
LDKLGFKDVNGDGYREFPDGKPLKLTIIVPFGWTDWMEMIRVISEQLAIVGINVEPKFPD
HHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHCEEEEEEECCCCCCC
YSKYWDDLTKGNFDMAINNFGSNVVATPWQWFNWVLNQDVTPIGENFYNGNFGRYKNERV
HHHHHHHHCCCCEEEEEECCCCCEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHH
AELLELINVEKDENKKLQYYYELQEIFLKDLPYIPIIYNGAWFEASPEHWTNWPTAKNPY
HHHHHHHCCCCCCCCCEEHHHHHHHHHHHHCCCCEEEECCCEECCCCCCCCCCCCCCCCE
AFPISWNGYWQMGGVKVLLNIKPVKAATETTTTEEKTETIVQTVTVTPTETATSSTETGG
EEEEECCCEEEECCEEEEEEEECCCCCCCCCCCHHHHHEEEEEEEECCCCCCCCCCCCCC
ICGPAILVGLAVVPLLLRRRRS
CHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRLVGVLIGAFVIFGVFGQVVAAQEQELPREETLYVGGGLWSQPNNFNPLIPWSAVTGT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCCCCCCCCCCCHHHHHH
IGLIYETLFNYDPLNDKLEPWLAESGRWVSDNVYEVKLREGLTWQDGKPLTAEDVKFTFE
HHHHHHHHHCCCCCCCCCCHHHHCCCCEECCCEEEEEEECCCCCCCCCCCCHHHEEEEEE
LHKKYPGLAYHQMWEWLQEVKVIDDRTIQFVFSTPHYEEWKYLLYQIAIVPKHIWQNIDN
EHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCC
PVEFANVDNPVGSGPYKLYKTDQMRFILVRNDNWWGIKYFGKPAPKYIVYVIVYSNNLAL
CEEECCCCCCCCCCCEEEEECCCEEEEEEECCCEEEEEECCCCCCCEEEEEEEEECCEEE
SMLVKGELDWSNFFIPGVPDVKAQYGIVTWYDEPPYHLPANTAFLFLNTQKPPLDNPEFR
EEEEECCCCCCCEECCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCCCCHHHH
RAIAYAINPEEIAARASQNQVTPADPTGLLIQYPAFKKIYNKEAVDKYGFKYDPQKAMEI
HHEEEECCHHHHHHHCCCCCCCCCCCCCEEEECCHHHHHHCHHHHHHCCCCCCHHHHHHH
LDKLGFKDVNGDGYREFPDGKPLKLTIIVPFGWTDWMEMIRVISEQLAIVGINVEPKFPD
HHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHCEEEEEEECCCCCCC
YSKYWDDLTKGNFDMAINNFGSNVVATPWQWFNWVLNQDVTPIGENFYNGNFGRYKNERV
HHHHHHHHCCCCEEEEEECCCCCEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHH
AELLELINVEKDENKKLQYYYELQEIFLKDLPYIPIIYNGAWFEASPEHWTNWPTAKNPY
HHHHHHHCCCCCCCCCEEHHHHHHHHHHHHCCCCEEEECCCEECCCCCCCCCCCCCCCCE
AFPISWNGYWQMGGVKVLLNIKPVKAATETTTTEEKTETIVQTVTVTPTETATSSTETGG
EEEEECCCEEEECCEEEEEEEECCCCCCCCCCCHHHHHEEEEEEEECCCCCCCCCCCCCC
ICGPAILVGLAVVPLLLRRRRS
CHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; peptides [Periplasm]; H2O [C]

Specific reaction: ATP + peptides [Periplasm] + H2O = ADP + phosphate + peptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]