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The map label for this gene is pmbA [C]

Identifier: 18977449

GI number: 18977449

Start: 1027500

End: 1028822

Strand: Direct

Name: pmbA [C]

Synonym: PF1077

Alternate gene names: 18977449

Gene position: 1027500-1028822 (Clockwise)

Preceding gene: 18977448

Following gene: 18977452

Centisome position: 53.84

GC content: 43.16

Gene sequence:

>1323_bases
ATGTTTGATGTTAACGAGCTCATACTTAAAAAGGCAAAGGAAATGGGATTTGGAGATGTTGTAGTCCTCAGTTACGAAAC
TAACAGGAGGCAAATAAGATTTGCAAACAATGAAGTTACTGTAGCTAAGCACTGGCATGAGCAGAAGGTCGAGGTATTCG
TTGAGATGGGAAAAAGGATAGCTTCAACGACGATTACAGAGCTAAGCCCCGAAAACATTGAAGCCACGTTAAAAGTTCTA
AAGGCAAACCTAGAGAAGATGAAGCCAAAAGAAGACTACTATGGAATTGCCGAGGGTCCCTTTAAGTACAAAGACATTCC
AGAGACATTTGATAAAGCTATAGTAGAGTTAGATGATCCAAGTGAGTATGTTGAAATTGCCATAAACGCAGCTCTAGAGG
AAGGAGCAAAGAGAGTGGCTGGAGTGCTTTATACAGATCACGACAAAATTTATCTAACCACCAGCAACGGTGTTGAGGCT
TTTGACGAGGGAACTGGAATAGAGATAAGCGTGAGAGCCTTTGTGGGCGAGTTAGAGAGCGGTCATGGAACTAATTCCGT
TAGGGTTCTTAAGAAGTTTGATCCAGAGAGTGCAGGAAGAAAAGCTGGAGAAATTGCAAAGCTCGCAAAGAATCCAGAAC
AAGGACCAGAAGGAAGGTTTGACGTTATATTTGATCCATTAGCCTTTGCCAATCTGTTAAGCTACATGGGATGGGCATTC
TCTGCATTCGCTGTTGAAGCTGGATTCAGCTACTTCGCCAACAAGCTCGACCAACAACTTGCCCCGGAGATCGTTACAAT
AAAAGATGTGGGTAACTTGCCCAATGGATATGGAACAAGAAAGTTTGACGACGAGGGAGTTCCAACTAAAGAAACGACTC
TCATAGATAAGGGAGTTCTGAAGACCTACCTACTAAACACGAGCTTTGCAAGGAAATACGGAAGAGAGACCACGGCAAAT
GCTGGGCTAATATCTCCAAGAGCTTGGAATATAGTCCTTGAGCCGGGTGATTACACAAAAGATGAGCTCTTCCAAGAGGT
AAAGAAGGGAATATACATCACAAACGTGTGGTACACAAGATTCCAGAACTACATGACTGGAGACTTCTCAACTATACCCA
GAGATGGAATATTCCTAATTGAGAACGGAGAGCTTAAACCAATAAGGAACATACGTGTGAGCGATAACATGCTCAGAATA
CTTTCAAATATACAAGGGTTGAGCAGGGAGTCATACCACATCCACTGGTGGGAAGTTAGCACACCAGTGTCAACACCCTA
CGTCCTCGTGAAAGATGTTGGAATAACAAGGGCAACCAAGTGA

Upstream 100 bases:

>100_bases
GTGGAAAAGGAGAACCAGGACAGGGAGTCCCAGTGTGGATGGGAGGAGCTCATGCGAGATTGAGGGATATTCCACTAAGA
AGGCCTTGAGGTGAGGGAAA

Downstream 100 bases:

>100_bases
TTCCTTTTTCTCTATTTTTTATAGGAGTAGGATGTATAGGAGACCAAGGACAAACAGTAACACTCCTGGGACTAGCAAGA
CCTTGTAAACCCTTCCTAAC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MFDVNELILKKAKEMGFGDVVVLSYETNRRQIRFANNEVTVAKHWHEQKVEVFVEMGKRIASTTITELSPENIEATLKVL
KANLEKMKPKEDYYGIAEGPFKYKDIPETFDKAIVELDDPSEYVEIAINAALEEGAKRVAGVLYTDHDKIYLTTSNGVEA
FDEGTGIEISVRAFVGELESGHGTNSVRVLKKFDPESAGRKAGEIAKLAKNPEQGPEGRFDVIFDPLAFANLLSYMGWAF
SAFAVEAGFSYFANKLDQQLAPEIVTIKDVGNLPNGYGTRKFDDEGVPTKETTLIDKGVLKTYLLNTSFARKYGRETTAN
AGLISPRAWNIVLEPGDYTKDELFQEVKKGIYITNVWYTRFQNYMTGDFSTIPRDGIFLIENGELKPIRNIRVSDNMLRI
LSNIQGLSRESYHIHWWEVSTPVSTPYVLVKDVGITRATK

Sequences:

>Translated_440_residues
MFDVNELILKKAKEMGFGDVVVLSYETNRRQIRFANNEVTVAKHWHEQKVEVFVEMGKRIASTTITELSPENIEATLKVL
KANLEKMKPKEDYYGIAEGPFKYKDIPETFDKAIVELDDPSEYVEIAINAALEEGAKRVAGVLYTDHDKIYLTTSNGVEA
FDEGTGIEISVRAFVGELESGHGTNSVRVLKKFDPESAGRKAGEIAKLAKNPEQGPEGRFDVIFDPLAFANLLSYMGWAF
SAFAVEAGFSYFANKLDQQLAPEIVTIKDVGNLPNGYGTRKFDDEGVPTKETTLIDKGVLKTYLLNTSFARKYGRETTAN
AGLISPRAWNIVLEPGDYTKDELFQEVKKGIYITNVWYTRFQNYMTGDFSTIPRDGIFLIENGELKPIRNIRVSDNMLRI
LSNIQGLSRESYHIHWWEVSTPVSTPYVLVKDVGITRATK
>Mature_440_residues
MFDVNELILKKAKEMGFGDVVVLSYETNRRQIRFANNEVTVAKHWHEQKVEVFVEMGKRIASTTITELSPENIEATLKVL
KANLEKMKPKEDYYGIAEGPFKYKDIPETFDKAIVELDDPSEYVEIAINAALEEGAKRVAGVLYTDHDKIYLTTSNGVEA
FDEGTGIEISVRAFVGELESGHGTNSVRVLKKFDPESAGRKAGEIAKLAKNPEQGPEGRFDVIFDPLAFANLLSYMGWAF
SAFAVEAGFSYFANKLDQQLAPEIVTIKDVGNLPNGYGTRKFDDEGVPTKETTLIDKGVLKTYLLNTSFARKYGRETTAN
AGLISPRAWNIVLEPGDYTKDELFQEVKKGIYITNVWYTRFQNYMTGDFSTIPRDGIFLIENGELKPIRNIRVSDNMLRI
LSNIQGLSRESYHIHWWEVSTPVSTPYVLVKDVGITRATK

Specific function: May Facilitate The Secretion Of The Peptide Antibiotic Microcin B17 (Mccb17) By Completing Its Maturation. Suppresses The Inhibitory Activity Of The Carbon Storage Regulator (Csra). [C]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=362, Percent_Identity=24.3093922651934, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1789640, Length=150, Percent_Identity=32, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 49490; Mature: 49490

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDVNELILKKAKEMGFGDVVVLSYETNRRQIRFANNEVTVAKHWHEQKVEVFVEMGKRI
CCCHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEECCEEEEEHHHHHHHHHHHHHHHHHH
ASTTITELSPENIEATLKVLKANLEKMKPKEDYYGIAEGPFKYKDIPETFDKAIVELDDP
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCHHHHHHHEEECCCC
SEYVEIAINAALEEGAKRVAGVLYTDHDKIYLTTSNGVEAFDEGTGIEISVRAFVGELES
HHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEEECCCCCHHCCCCCCEEEHHHHHHHHCC
GHGTNSVRVLKKFDPESAGRKAGEIAKLAKNPEQGPEGRFDVIFDPLAFANLLSYMGWAF
CCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHHHHHHH
SAFAVEAGFSYFANKLDQQLAPEIVTIKDVGNLPNGYGTRKFDDEGVPTKETTLIDKGVL
HHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
KTYLLNTSFARKYGRETTANAGLISPRAWNIVLEPGDYTKDELFQEVKKGIYITNVWYTR
HHHHHCCHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCEEEEEHHHHH
FQNYMTGDFSTIPRDGIFLIENGELKPIRNIRVSDNMLRILSNIQGLSRESYHIHWWEVS
HHHHCCCCCCCCCCCCEEEEECCCCCCCCCEECCHHHHHHHHHHHCCCCCCEEEEEEEEC
TPVSTPYVLVKDVGITRATK
CCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MFDVNELILKKAKEMGFGDVVVLSYETNRRQIRFANNEVTVAKHWHEQKVEVFVEMGKRI
CCCHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEECCEEEEEHHHHHHHHHHHHHHHHHH
ASTTITELSPENIEATLKVLKANLEKMKPKEDYYGIAEGPFKYKDIPETFDKAIVELDDP
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCHHHHHHHEEECCCC
SEYVEIAINAALEEGAKRVAGVLYTDHDKIYLTTSNGVEAFDEGTGIEISVRAFVGELES
HHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEEECCCCCHHCCCCCCEEEHHHHHHHHCC
GHGTNSVRVLKKFDPESAGRKAGEIAKLAKNPEQGPEGRFDVIFDPLAFANLLSYMGWAF
CCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHHHHHHH
SAFAVEAGFSYFANKLDQQLAPEIVTIKDVGNLPNGYGTRKFDDEGVPTKETTLIDKGVL
HHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
KTYLLNTSFARKYGRETTANAGLISPRAWNIVLEPGDYTKDELFQEVKKGIYITNVWYTR
HHHHHCCHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCEEEEEHHHHH
FQNYMTGDFSTIPRDGIFLIENGELKPIRNIRVSDNMLRILSNIQGLSRESYHIHWWEVS
HHHHCCCCCCCCCCCCEEEEECCCCCCCCCEECCHHHHHHHHHHHCCCCCCEEEEEEEEC
TPVSTPYVLVKDVGITRATK
CCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]