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The map label for this gene is tgtA

Identifier: 18977418

GI number: 18977418

Start: 998603

End: 1000360

Strand: Direct

Name: tgtA

Synonym: PF1046

Alternate gene names: 18977418

Gene position: 998603-1000360 (Clockwise)

Preceding gene: 18977417

Following gene: 18977419

Centisome position: 52.33

GC content: 41.92

Gene sequence:

>1758_bases
ATGAGCCGAGGTGATAGTATGCTAAGATTTGAAATAAAGGACAGGGATGCTGCTGGAAGGATTGGAAAATTGGAGGTCAA
TGGAAAGAAGATAGAGACTCCAGCTATAATGCCAGTTGTTAACCCTAAGCAGCTTATAGTTGAACCAAAGGAACTAAAGA
GAATGGGATTTGATATAATAATAACAAACTCCTATATAATCTATAAAGACAAAAAACTGAGGGAAAAGGCTTTAGAAAAA
GGCATACATAGGCTTTTAGACTACGATGGCATAATAGAGGTTGACTCAGGCTCATTCCAACTTATGAGGTATGGAAAAGT
CGAAGTTACGAACAGGGAAATAGTCGAGTTTCAGCACAAAATTGGGGTTGACATAGGGACTTTTCTCGACATCCCTACAC
CCCCAGATGCTCCCAGGGAAAAGGCCGAGCAGGATTTGAAGATCACCCTAGAAAGGGCAAAAGAAGCTGAAAGTATAAAG
CAAATTCCAATGAATGCCACAGTTCAAGGTTCAACATACCTGGACTTAAGGAAACTTGCCGCAAGAAAGCTAAGCGAAAT
GAACTTTGAAATTCATCCAATTGGAGCTGTTGTTCCATTGCTAGAGAGTTATAGATTTAAGGACGTTGTGGACATCGTTA
TCGCATCTAAAATGGGCCTTAGGCCAGATAGGCCTGTTCACCTTTTCGGAGCTGGCCATCCAATGGTTTTTGCTTTAGCA
GTTGCTATGGGAGTTGACCTGTTTGACTCCGCAAGTTATGCACTTTATGCAAAAGATGATAGATACTTAACTCCCCAGGG
GACTAAGAGATTAGAAGAGCTCGAATATTTTTCCTGCTCCTGTCCCGTTTGTTCTAAATACACTCCGCAAGAACTAAGAG
AAATGCCAAAAGAAGAAAGGGAAAAATTACTTGCTCTCCACAATCTGTGGGTTATTAGAGAGGAAATTAATAGAGTAAAA
CAGGCAATAAAGGAGGGTGAGCTTTGGAGGCTCGTTGATGAGAGGGCAAGAGCTCATCCAAAGCTATATGCCGCTTACAA
AAGGCTTCTGGAGTATTATCATTATTTGGAGGAATACGAACCAATAACTAAGAAGTCCGCATTCTTTAAAATAAGTGAGG
AAAGCCTAAAATGGCCAATTGCAAGAAGGGCAAAAGAAAGAGCAGAAAAGGTAAAGGCAAAGTTCCCCGAAAGCATTCCC
CATCCAATATTTGGGGAGATTCCCAAATATCTTAGCCTAACATATCCCTTTGCTCAAAGCGAGAGTGAGGAAGACTTTCA
AATAGAAAAACCAACAAGAGAGAACGCAATCCTCTACATAATGGCAATAGCCGAGTACCAGTTTGGTGAAGGAGCAGGAG
AAGCATTTAGAGACGCTGAAGTTGAGATAGCAAAGACGGGAATGCCTAGGCAGGTAAAGAAAAATGGAAAAAGGCTCGCC
ACTGTAAGAGCTGAAGACGGATTGCTAACCCTGGGAATTGAAGGTGCTAAAAGGCTCCACGAACTTCTCCCATATCCAGT
GATGAGAGTTGTGGTTAATAAGGAAGCTGAACCATTTGCCAGGAAAGGAAAAGACGTCTTCGCCAAGTTCGTAGAATTTG
CAGATCCTAAAATCAGGCCATATGACGAGGTTCTGATTGTTAATGAAAACGATGAGTTACTTGCAACGGGGCAAGCTCTA
CTTTCGGGCAGAGAAATGGTTTTGTTCAGCTCAGGAAGAGCCGTTAAAACAAGAAGAGGTGTAGAGGAGAAAAAGTGA

Upstream 100 bases:

>100_bases
GAAGTATGGGAGAGAATAAGGCCTCTATTAGATTACATCCGCAATAGGGAGGCGATGATTTGACTCCGGAAAATCTGATC
GATGATGAAGTCCAGCCCGA

Downstream 100 bases:

>100_bases
TATACCCTAATAACTAATCCTATCTAGGGGATAAAGTATGGAGTTCAACATGTTTGGCATAACAGGGGATGTAGGCGTTG
GTGCTGTAGTGGGATTCATA

Product: 7-cyano-7-deazaguanine tRNA-ribosyltransferase

Products: tRNAqueuine; guanine [C]

Alternate protein names: Archaeal tRNA-guanine transglycosylase

Number of amino acids: Translated: 585; Mature: 584

Protein sequence:

>585_residues
MSRGDSMLRFEIKDRDAAGRIGKLEVNGKKIETPAIMPVVNPKQLIVEPKELKRMGFDIIITNSYIIYKDKKLREKALEK
GIHRLLDYDGIIEVDSGSFQLMRYGKVEVTNREIVEFQHKIGVDIGTFLDIPTPPDAPREKAEQDLKITLERAKEAESIK
QIPMNATVQGSTYLDLRKLAARKLSEMNFEIHPIGAVVPLLESYRFKDVVDIVIASKMGLRPDRPVHLFGAGHPMVFALA
VAMGVDLFDSASYALYAKDDRYLTPQGTKRLEELEYFSCSCPVCSKYTPQELREMPKEEREKLLALHNLWVIREEINRVK
QAIKEGELWRLVDERARAHPKLYAAYKRLLEYYHYLEEYEPITKKSAFFKISEESLKWPIARRAKERAEKVKAKFPESIP
HPIFGEIPKYLSLTYPFAQSESEEDFQIEKPTRENAILYIMAIAEYQFGEGAGEAFRDAEVEIAKTGMPRQVKKNGKRLA
TVRAEDGLLTLGIEGAKRLHELLPYPVMRVVVNKEAEPFARKGKDVFAKFVEFADPKIRPYDEVLIVNENDELLATGQAL
LSGREMVLFSSGRAVKTRRGVEEKK

Sequences:

>Translated_585_residues
MSRGDSMLRFEIKDRDAAGRIGKLEVNGKKIETPAIMPVVNPKQLIVEPKELKRMGFDIIITNSYIIYKDKKLREKALEK
GIHRLLDYDGIIEVDSGSFQLMRYGKVEVTNREIVEFQHKIGVDIGTFLDIPTPPDAPREKAEQDLKITLERAKEAESIK
QIPMNATVQGSTYLDLRKLAARKLSEMNFEIHPIGAVVPLLESYRFKDVVDIVIASKMGLRPDRPVHLFGAGHPMVFALA
VAMGVDLFDSASYALYAKDDRYLTPQGTKRLEELEYFSCSCPVCSKYTPQELREMPKEEREKLLALHNLWVIREEINRVK
QAIKEGELWRLVDERARAHPKLYAAYKRLLEYYHYLEEYEPITKKSAFFKISEESLKWPIARRAKERAEKVKAKFPESIP
HPIFGEIPKYLSLTYPFAQSESEEDFQIEKPTRENAILYIMAIAEYQFGEGAGEAFRDAEVEIAKTGMPRQVKKNGKRLA
TVRAEDGLLTLGIEGAKRLHELLPYPVMRVVVNKEAEPFARKGKDVFAKFVEFADPKIRPYDEVLIVNENDELLATGQAL
LSGREMVLFSSGRAVKTRRGVEEKK
>Mature_584_residues
SRGDSMLRFEIKDRDAAGRIGKLEVNGKKIETPAIMPVVNPKQLIVEPKELKRMGFDIIITNSYIIYKDKKLREKALEKG
IHRLLDYDGIIEVDSGSFQLMRYGKVEVTNREIVEFQHKIGVDIGTFLDIPTPPDAPREKAEQDLKITLERAKEAESIKQ
IPMNATVQGSTYLDLRKLAARKLSEMNFEIHPIGAVVPLLESYRFKDVVDIVIASKMGLRPDRPVHLFGAGHPMVFALAV
AMGVDLFDSASYALYAKDDRYLTPQGTKRLEELEYFSCSCPVCSKYTPQELREMPKEEREKLLALHNLWVIREEINRVKQ
AIKEGELWRLVDERARAHPKLYAAYKRLLEYYHYLEEYEPITKKSAFFKISEESLKWPIARRAKERAEKVKAKFPESIPH
PIFGEIPKYLSLTYPFAQSESEEDFQIEKPTRENAILYIMAIAEYQFGEGAGEAFRDAEVEIAKTGMPRQVKKNGKRLAT
VRAEDGLLTLGIEGAKRLHELLPYPVMRVVVNKEAEPFARKGKDVFAKFVEFADPKIRPYDEVLIVNENDELLATGQALL
SGREMVLFSSGRAVKTRRGVEEKK

Specific function: Exchanges the guanine residue with 7-cyano-7- deazaguanine (preQ0) at position 15 in the dihydrouridine loop (D- loop) of archaeal tRNAs

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Escherichia coli, GI1786607, Length=366, Percent_Identity=23.7704918032787, Blast_Score=108, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17544680, Length=358, Percent_Identity=24.3016759776536, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI19920510, Length=357, Percent_Identity=23.249299719888, Blast_Score=83, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ATGT_PYRFU (Q8TH08)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_578775.1
- ProteinModelPortal:   Q8TH08
- SMR:   Q8TH08
- EnsemblBacteria:   EBPYRT00000005787
- GeneID:   1468912
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1046
- NMPDR:   fig|186497.1.peg.1083
- GeneTree:   EBGT00050000022193
- HOGENOM:   HBG512800
- OMA:   VETPTVM
- ProtClustDB:   PRK13534
- HAMAP:   MF_01634
- InterPro:   IPR004804
- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR002616
- InterPro:   IPR004521
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- SMART:   SM00359
- TIGRFAMs:   TIGR00432
- TIGRFAMs:   TIGR00449
- TIGRFAMs:   TIGR00451

Pfam domain/function: PF01472 PUA; PF01702 TGT; SSF88697 PUA-like; SSF51713 tRNA_ribo_trans

EC number: 2.4.2.29 [C]

Molecular weight: Translated: 66941; Mature: 66810

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS50890 PUA

Important sites: ACT_SITE 249-249 BINDING 250-250

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRGDSMLRFEIKDRDAAGRIGKLEVNGKKIETPAIMPVVNPKQLIVEPKELKRMGFDII
CCCCCCEEEEEEECCCCCCCEEEEEECCEEECCCEEEECCCCHHHEECHHHHHHCCCEEE
ITNSYIIYKDKKLREKALEKGIHRLLDYDGIIEVDSGSFQLMRYGKVEVTNREIVEFQHK
EECCEEEEECHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEECEEEECCHHHHHHHHH
IGVDIGTFLDIPTPPDAPREKAEQDLKITLERAKEAESIKQIPMNATVQGSTYLDLRKLA
HCCCHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHH
ARKLSEMNFEIHPIGAVVPLLESYRFKDVVDIVIASKMGLRPDRPVHLFGAGHPMVFALA
HHHHHHCCCEEEEHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHH
VAMGVDLFDSASYALYAKDDRYLTPQGTKRLEELEYFSCSCPVCSKYTPQELREMPKEER
HHHCCHHHCCCCEEEEECCCCEECCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCHHHH
EKLLALHNLWVIREEINRVKQAIKEGELWRLVDERARAHPKLYAAYKRLLEYYHYLEEYE
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
PITKKSAFFKISEESLKWPIARRAKERAEKVKAKFPESIPHPIFGEIPKYLSLTYPFAQS
CCCCCCCEEEECHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHEEECCCCCC
ESEEDFQIEKPTRENAILYIMAIAEYQFGEGAGEAFRDAEVEIAKTGMPRQVKKNGKRLA
CCCCCCEECCCCCCCEEEEEEEEEEECCCCCCCHHHHCCCEEEECCCCCHHHHHCCCEEE
TVRAEDGLLTLGIEGAKRLHELLPYPVMRVVVNKEAEPFARKGKDVFAKFVEFADPKIRP
EEEECCCEEEEECHHHHHHHHHCCHHHHHHHHCCCCCHHHHCCHHHHHHHHHHCCCCCCC
YDEVLIVNENDELLATGQALLSGREMVLFSSGRAVKTRRGVEEKK
CCCEEEECCCCCEEECCHHHHCCCEEEEEECCCEEHHHCCCCCCC
>Mature Secondary Structure 
SRGDSMLRFEIKDRDAAGRIGKLEVNGKKIETPAIMPVVNPKQLIVEPKELKRMGFDII
CCCCCEEEEEEECCCCCCCEEEEEECCEEECCCEEEECCCCHHHEECHHHHHHCCCEEE
ITNSYIIYKDKKLREKALEKGIHRLLDYDGIIEVDSGSFQLMRYGKVEVTNREIVEFQHK
EECCEEEEECHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEECEEEECCHHHHHHHHH
IGVDIGTFLDIPTPPDAPREKAEQDLKITLERAKEAESIKQIPMNATVQGSTYLDLRKLA
HCCCHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHH
ARKLSEMNFEIHPIGAVVPLLESYRFKDVVDIVIASKMGLRPDRPVHLFGAGHPMVFALA
HHHHHHCCCEEEEHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHH
VAMGVDLFDSASYALYAKDDRYLTPQGTKRLEELEYFSCSCPVCSKYTPQELREMPKEER
HHHCCHHHCCCCEEEEECCCCEECCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCHHHH
EKLLALHNLWVIREEINRVKQAIKEGELWRLVDERARAHPKLYAAYKRLLEYYHYLEEYE
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
PITKKSAFFKISEESLKWPIARRAKERAEKVKAKFPESIPHPIFGEIPKYLSLTYPFAQS
CCCCCCCEEEECHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHEEECCCCCC
ESEEDFQIEKPTRENAILYIMAIAEYQFGEGAGEAFRDAEVEIAKTGMPRQVKKNGKRLA
CCCCCCEECCCCCCCEEEEEEEEEEECCCCCCCHHHHCCCEEEECCCCCHHHHHCCCEEE
TVRAEDGLLTLGIEGAKRLHELLPYPVMRVVVNKEAEPFARKGKDVFAKFVEFADPKIRP
EEEECCCEEEEECHHHHHHHHHCCHHHHHHHHCCCCCHHHHCCHHHHHHHHHHCCCCCCC
YDEVLIVNENDELLATGQALLSGREMVLFSSGRAVKTRRGVEEKK
CCCEEEECCCCCEEECCHHHHCCCEEEEEECCCEEHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Zn; Mg [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.00006 {guanine}} 0.000053 {guanine}} 0.000014 {7-(aminomethyl)-7-deazaguanine}} [C]

Substrates: tRNAguanine; queuine [C]

Specific reaction: tRNAguanine + queuine = tRNAqueuine + guanine [C]

General reaction: Pentosyl group transfer [C]

Inhibitor: Bisimidoester; Dimethyl suberimidate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA