BacMap

Definition	Pyrococcus furiosus DSM 3638, complete genome.
Accession	NC_003413
Length	1,908,256

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The map label for this gene is metG

Identifier: 18977402

GI number: 18977402

Start: 985542

End: 987716

Strand: Direct

Name: metG

Synonym: PF1030

Alternate gene names: 18977402

Gene position: 985542-987716 (Clockwise)

Preceding gene: 18977401

Following gene: 18977407

Centisome position: 51.65

GC content: 38.67

Gene sequence:

>2175_bases
ATGGTAAGATATATGGTTACAGCCGCTTTACCGTATGCTAATGGACCAATTCATGCGGGGCATTTAGCCGGAGCTTATTT
GCCAGCTGATATTTTTGTAAGATATCTAAGGCTCAAAGGAGAAGATGTTGTGTTCATATGTGGGACTGATGAACATGGAA
CTCCAATTTCTTTTAGAGCATTAAATGAGAAGAGAAGTCCCAGGGAAATTGTTGATGAGTTTCATGAACACATAAAAACA
GCATTTCAGAGGGTCAAGATAAGCTTTGATTACTTTGGAAGAACGGAGCTTCCAGTTCACTATAGGCTAAGTCAAGAATT
CTTCCTGAAAGCTTTAGAAAATGGCTATCTTGTGAAGAAAGTAACAAAACAAGCATATTGTGAGCACGACAAAAGATTTC
TTCCAGATAGATTCGTTATTGGGACATGTCCATACTGTGGAGCAGAAAACCAAAGAGGAGATCAGTGTGAGGTATGTGGG
AGACCTCTTACGCCTGAAATTCTAATAGAACCTAGATGTGCCTTCTGTAAGAATCCAATAACCTTTAGAGAATCCACTCA
TTACTACATAAAAATGCAGGAGTTTGAAGAGAAACTTAAAGAGTGGATAAAGGAGAAAGATTGGAAGCCAAATGTAAAGA
ATATGGTGCTGGGATGGATAGAAGAAGGATTGGAAGAAAGAGCTATAACGAGAGATCTAGATTGGGGAATTCCAGTTCCT
TTGGATGAAGAAGACATGAAGAATAAAGTGCTGTACGTTTGGTTTGAAGCTCCTATTGGATATATTTCACTTACGATAGA
ATACTTCAAGAGAATCGGAAAACCAAATGAGTGGAAGAAATACTGGTTGAATTTAGATGGTCAAACTAGGGTTATCCACT
TTATTGGAAAAGACAACATACCATTCCACGCGATATTCTGGCCGGCATTTCTCATGGCCTATGGAAAGTATAAGGATGAA
GAAGTTGAGGCAGAATGGAATCTTCCCTATGATATCCCAGCAAATGAGTATTTAACACTAGAAGGAAAGAAGTTCTCAAC
AAGCAGAAACTGGGCTATATGGATTCATGAATTCCTTGATGTCTTCCCGGCAGATTATCTTAGGTATTATTTGACCTCAA
TAATGCCTGAAACTAGAGATTCAGACTTTAGCTTTGCAGAGTTCAAGACAAAGATCAATGAGGAGCTTGTCAATGTTCTT
GGCAACTTTGTGTACAGAGCGCTAACCTTCGTTAACAGATACTTCGATGGAATAGTTCCTGAAAGAGGAGAGCTAGATGA
ATTGGATAGGCAAGCCTTAGAGGAAATTGAAAAAACGTTTGAAGAAGTTGGCAAGCTTATAAGGGAGTACAGATTTAAAG
ATGCCTTAAAGAAGGTTATGAATCTAGCAGGTTTTGGCAACAGATACTTTGATTACAAAGAGCCATGGAAGACAATAAAG
GAGGATAAAACAAGAACAGGCACTACAATTAACATTTCCCTACAAATAGTGAAGGCCCTGGGAATATTATTAGAACCCTT
CCTTCCCGATGCAAGCGAAAAGATATGGCATTTCCTAAACCTAGAGGAAGTTAAAACGTGGAGATTTACAGAGCTCCCAG
CTGGACATAAAGTGAGAAAGCCTGAAATATTGTTCAAGAAAGCATCCGATGACCAGATAATTTACTTTATCCTTAACTAC
ATGGGAAGGAACAATCCAGACGCTGCAAGAGAATTGCTCGAAAAGTATTACAAACTTGAAGATGTGGAGAAAGTTGCAAT
AGAAAAATTTGGAGAAGAGGACGGAAGATTCATGCTTAAGAAAATATTTAAAGAGAAATACAAGGGAGAAAAAAAGGGTG
AGCAAAAAATGCAATACGTAAGCTTTGAGGAGTTTTCAAAGATTGATCTTAGGATAGGAAAGATCGTTGAAGTTCAAGAC
CATCCAAATGCAGATAAGCTTTATGTGGTAAAGGTAGATCTTGGAGGGGAGATTAGAACTCTAGTTGCAGGACTCAAAAA
GTACTATAAACCAGAAGAGTTGCTAAACAGATATGTTGTTATAGTGGCGAACTTAGAACCTAAGAAGCTAAGAGGAGTTG
AGAGCCAAGGAATGCTACTAGCCGCGGATGATGGTGAGAAAGTTGCACTGTTAATGCCAGATAAGGAAGTAAAGCTGGGA
GCAAAAGTTAGGTAG

Upstream 100 bases:

>100_bases
GGAGGATATGCAAAAATTAATATAAAGCATGTAGTTTAAAATAACCCATCGTAATATTTAAATTTGTCAATTTCAGAAAG
AGCACAAAGGAGGTGATAAA

Downstream 100 bases:

>100_bases
ATTTCGTTTCCTCCAATAACCATTTTAAGTATTCCTCGTTTACATCCTCTACATTAATTTTTATTATTGCTGGGACAGTG
TATGGATGGAGCTCTTTTAT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 724; Mature: 724

Protein sequence:

>724_residues
MVRYMVTAALPYANGPIHAGHLAGAYLPADIFVRYLRLKGEDVVFICGTDEHGTPISFRALNEKRSPREIVDEFHEHIKT
AFQRVKISFDYFGRTELPVHYRLSQEFFLKALENGYLVKKVTKQAYCEHDKRFLPDRFVIGTCPYCGAENQRGDQCEVCG
RPLTPEILIEPRCAFCKNPITFRESTHYYIKMQEFEEKLKEWIKEKDWKPNVKNMVLGWIEEGLEERAITRDLDWGIPVP
LDEEDMKNKVLYVWFEAPIGYISLTIEYFKRIGKPNEWKKYWLNLDGQTRVIHFIGKDNIPFHAIFWPAFLMAYGKYKDE
EVEAEWNLPYDIPANEYLTLEGKKFSTSRNWAIWIHEFLDVFPADYLRYYLTSIMPETRDSDFSFAEFKTKINEELVNVL
GNFVYRALTFVNRYFDGIVPERGELDELDRQALEEIEKTFEEVGKLIREYRFKDALKKVMNLAGFGNRYFDYKEPWKTIK
EDKTRTGTTINISLQIVKALGILLEPFLPDASEKIWHFLNLEEVKTWRFTELPAGHKVRKPEILFKKASDDQIIYFILNY
MGRNNPDAARELLEKYYKLEDVEKVAIEKFGEEDGRFMLKKIFKEKYKGEKKGEQKMQYVSFEEFSKIDLRIGKIVEVQD
HPNADKLYVVKVDLGGEIRTLVAGLKKYYKPEELLNRYVVIVANLEPKKLRGVESQGMLLAADDGEKVALLMPDKEVKLG
AKVR

Sequences:

>Translated_724_residues
MVRYMVTAALPYANGPIHAGHLAGAYLPADIFVRYLRLKGEDVVFICGTDEHGTPISFRALNEKRSPREIVDEFHEHIKT
AFQRVKISFDYFGRTELPVHYRLSQEFFLKALENGYLVKKVTKQAYCEHDKRFLPDRFVIGTCPYCGAENQRGDQCEVCG
RPLTPEILIEPRCAFCKNPITFRESTHYYIKMQEFEEKLKEWIKEKDWKPNVKNMVLGWIEEGLEERAITRDLDWGIPVP
LDEEDMKNKVLYVWFEAPIGYISLTIEYFKRIGKPNEWKKYWLNLDGQTRVIHFIGKDNIPFHAIFWPAFLMAYGKYKDE
EVEAEWNLPYDIPANEYLTLEGKKFSTSRNWAIWIHEFLDVFPADYLRYYLTSIMPETRDSDFSFAEFKTKINEELVNVL
GNFVYRALTFVNRYFDGIVPERGELDELDRQALEEIEKTFEEVGKLIREYRFKDALKKVMNLAGFGNRYFDYKEPWKTIK
EDKTRTGTTINISLQIVKALGILLEPFLPDASEKIWHFLNLEEVKTWRFTELPAGHKVRKPEILFKKASDDQIIYFILNY
MGRNNPDAARELLEKYYKLEDVEKVAIEKFGEEDGRFMLKKIFKEKYKGEKKGEQKMQYVSFEEFSKIDLRIGKIVEVQD
HPNADKLYVVKVDLGGEIRTLVAGLKKYYKPEELLNRYVVIVANLEPKKLRGVESQGMLLAADDGEKVALLMPDKEVKLG
AKVR
>Mature_724_residues
MVRYMVTAALPYANGPIHAGHLAGAYLPADIFVRYLRLKGEDVVFICGTDEHGTPISFRALNEKRSPREIVDEFHEHIKT
AFQRVKISFDYFGRTELPVHYRLSQEFFLKALENGYLVKKVTKQAYCEHDKRFLPDRFVIGTCPYCGAENQRGDQCEVCG
RPLTPEILIEPRCAFCKNPITFRESTHYYIKMQEFEEKLKEWIKEKDWKPNVKNMVLGWIEEGLEERAITRDLDWGIPVP
LDEEDMKNKVLYVWFEAPIGYISLTIEYFKRIGKPNEWKKYWLNLDGQTRVIHFIGKDNIPFHAIFWPAFLMAYGKYKDE
EVEAEWNLPYDIPANEYLTLEGKKFSTSRNWAIWIHEFLDVFPADYLRYYLTSIMPETRDSDFSFAEFKTKINEELVNVL
GNFVYRALTFVNRYFDGIVPERGELDELDRQALEEIEKTFEEVGKLIREYRFKDALKKVMNLAGFGNRYFDYKEPWKTIK
EDKTRTGTTINISLQIVKALGILLEPFLPDASEKIWHFLNLEEVKTWRFTELPAGHKVRKPEILFKKASDDQIIYFILNY
MGRNNPDAARELLEKYYKLEDVEKVAIEKFGEEDGRFMLKKIFKEKYKGEKKGEQKMQYVSFEEFSKIDLRIGKIVEVQD
HPNADKLYVVKVDLGGEIRTLVAGLKKYYKPEELLNRYVVIVANLEPKKLRGVESQGMLLAADDGEKVALLMPDKEVKLG
AKVR

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=562, Percent_Identity=37.3665480427046, Blast_Score=407, Evalue=1e-113,
Organism=Homo sapiens, GI24308436, Length=541, Percent_Identity=26.9870609981516, Blast_Score=176, Evalue=6e-44,
Organism=Homo sapiens, GI4507947, Length=79, Percent_Identity=55.6962025316456, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI215490011, Length=92, Percent_Identity=46.7391304347826, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI215490009, Length=92, Percent_Identity=46.7391304347826, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI45006986, Length=92, Percent_Identity=46.7391304347826, Blast_Score=87, Evalue=5e-17,
Organism=Escherichia coli, GI1788432, Length=729, Percent_Identity=31.4128943758573, Blast_Score=373, Evalue=1e-104,
Organism=Escherichia coli, GI1789455, Length=109, Percent_Identity=44.0366972477064, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786861, Length=228, Percent_Identity=24.5614035087719, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17540794, Length=622, Percent_Identity=35.048231511254, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI212645096, Length=522, Percent_Identity=27.9693486590038, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI71992781, Length=419, Percent_Identity=26.0143198090692, Blast_Score=119, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6321704, Length=575, Percent_Identity=34.4347826086956, Blast_Score=360, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6321610, Length=545, Percent_Identity=24.0366972477064, Blast_Score=118, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6321333, Length=100, Percent_Identity=42, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24655311, Length=558, Percent_Identity=38.1720430107527, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI21358483, Length=532, Percent_Identity=24.6240601503759, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24651924, Length=91, Percent_Identity=49.4505494505495, Blast_Score=93, Evalue=6e-19,
Organism=Drosophila melanogaster, GI21357223, Length=81, Percent_Identity=45.679012345679, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_PYRFU (Q8U221)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_578759.1
- ProteinModelPortal:   Q8U221
- EnsemblBacteria:   EBPYRT00000005775
- GeneID:   1468896
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1030
- NMPDR:   fig|186497.1.peg.1067
- GeneTree:   EBGT00070000032183
- HOGENOM:   HBG721667
- OMA:   SAYQPEQ
- ProtClustDB:   PRK00133
- BRENDA:   6.1.1.10
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 84992; Mature: 84992

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 347-347

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRYMVTAALPYANGPIHAGHLAGAYLPADIFVRYLRLKGEDVVFICGTDEHGTPISFRA
CCEEEEEEECCCCCCCCCCCHHCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEE
LNEKRSPREIVDEFHEHIKTAFQRVKISFDYFGRTELPVHYRLSQEFFLKALENGYLVKK
CCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCEEEECCHHHHHHHHHCCHHHHH
VTKQAYCEHDKRFLPDRFVIGTCPYCGAENQRGDQCEVCGRPLTPEILIEPRCAFCKNPI
HHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHCCCCCCCCEEECCCHHHHCCCC
TFRESTHYYIKMQEFEEKLKEWIKEKDWKPNVKNMVLGWIEEGLEERAITRDLDWGIPVP
EEECCCEEEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LDEEDMKNKVLYVWFEAPIGYISLTIEYFKRIGKPNEWKKYWLNLDGQTRVIHFIGKDNI
CCHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCHHEEECCCCCEEEEEEECCCCC
PFHAIFWPAFLMAYGKYKDEEVEAEWNLPYDIPANEYLTLEGKKFSTSRNWAIWIHEFLD
CEEHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCEEEEECCEECCCCCCEEHHHHHHH
VFPADYLRYYLTSIMPETRDSDFSFAEFKTKINEELVNVLGNFVYRALTFVNRYFDGIVP
HCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ERGELDELDRQALEEIEKTFEEVGKLIREYRFKDALKKVMNLAGFGNRYFDYKEPWKTIK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
EDKTRTGTTINISLQIVKALGILLEPFLPDASEKIWHFLNLEEVKTWRFTELPAGHKVRK
HCCCCCCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHCCEEEEECCCCCCCCC
PEILFKKASDDQIIYFILNYMGRNNPDAARELLEKYYKLEDVEKVAIEKFGEEDGRFMLK
CHHEEEECCCCCEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
KIFKEKYKGEKKGEQKMQYVSFEEFSKIDLRIGKIVEVQDHPNADKLYVVKVDLGGEIRT
HHHHHHHCCCCCHHHHHHHCCHHHHHHHEEEECEEEEECCCCCCCEEEEEEEECCCHHHH
LVAGLKKYYKPEELLNRYVVIVANLEPKKLRGVESQGMLLAADDGEKVALLMPDKEVKLG
HHHHHHHHCCHHHHCCCEEEEEECCCHHHHCCCCCCCEEEEECCCCEEEEEECCCCCCCC
AKVR
CCCC
>Mature Secondary Structure
MVRYMVTAALPYANGPIHAGHLAGAYLPADIFVRYLRLKGEDVVFICGTDEHGTPISFRA
CCEEEEEEECCCCCCCCCCCHHCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEE
LNEKRSPREIVDEFHEHIKTAFQRVKISFDYFGRTELPVHYRLSQEFFLKALENGYLVKK
CCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCEEEECCHHHHHHHHHCCHHHHH
VTKQAYCEHDKRFLPDRFVIGTCPYCGAENQRGDQCEVCGRPLTPEILIEPRCAFCKNPI
HHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHCCCCCCCCEEECCCHHHHCCCC
TFRESTHYYIKMQEFEEKLKEWIKEKDWKPNVKNMVLGWIEEGLEERAITRDLDWGIPVP
EEECCCEEEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LDEEDMKNKVLYVWFEAPIGYISLTIEYFKRIGKPNEWKKYWLNLDGQTRVIHFIGKDNI
CCHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCHHEEECCCCCEEEEEEECCCCC
PFHAIFWPAFLMAYGKYKDEEVEAEWNLPYDIPANEYLTLEGKKFSTSRNWAIWIHEFLD
CEEHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCEEEEECCEECCCCCCEEHHHHHHH
VFPADYLRYYLTSIMPETRDSDFSFAEFKTKINEELVNVLGNFVYRALTFVNRYFDGIVP
HCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ERGELDELDRQALEEIEKTFEEVGKLIREYRFKDALKKVMNLAGFGNRYFDYKEPWKTIK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
EDKTRTGTTINISLQIVKALGILLEPFLPDASEKIWHFLNLEEVKTWRFTELPAGHKVRK
HCCCCCCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHCCEEEEECCCCCCCCC
PEILFKKASDDQIIYFILNYMGRNNPDAARELLEKYYKLEDVEKVAIEKFGEEDGRFMLK
CHHEEEECCCCCEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
KIFKEKYKGEKKGEQKMQYVSFEEFSKIDLRIGKIVEVQDHPNADKLYVVKVDLGGEIRT
HHHHHHHCCCCCHHHHHHHCCHHHHHHHEEEECEEEEECCCCCCCEEEEEEEECCCHHHH
LVAGLKKYYKPEELLNRYVVIVANLEPKKLRGVESQGMLLAADDGEKVALLMPDKEVKLG
HHHHHHHHCCHHHHCCCEEEEEECCCHHHHCCCCCCCEEEEECCCCEEEEEECCCCCCCC
AKVR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA