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The map label for this gene is ytsJ [H]

Identifier: 18977398

GI number: 18977398

Start: 981072

End: 982379

Strand: Reverse

Name: ytsJ [H]

Synonym: PF1026

Alternate gene names: 18977398

Gene position: 982379-981072 (Counterclockwise)

Preceding gene: 18977399

Following gene: 18977397

Centisome position: 51.48

GC content: 41.51

Gene sequence:

>1308_bases
ATGGCTAGGATCACTGAGGAACAAAGAAGGAAGCTTGCTCAAGATGCGTTGGAGTATCACAGAAATAATTTCCCAGGGAA
TGGAAAAATTGAAGTGATACCAAAGGTTCCTCTTAGAGATTTCTATGATTTGAGCTTAGCATATACCCCAGGAGTAGCGG
AGCCATGTAAAGTAATTGCAAAGAAGGGAAACTATGAAGATTACACTATAATTCCAAATACAGTTGCCGTCGTTACAGAC
GGATCGGCAATCTTGGGATTAGGAAACATAGGTGTTCTGGCAGGAATGCCAGTTATGGAAGGTAAATGTGTTCTCTTTAA
AGCTCTAGCTGGAATAGATGCGTTTCCTATCTTAATTGACACACAGGATGTCGAGAAAATCGTGGAAACAATAAAGCTTA
TATCAAAGGGATTTGGCGGGATAAACCTAGAAGATATCTCCGCTCCAAGATGCTTCGAAATTGAAATGAGGTTGAAGAGA
GAGCTTGATATTCCCGTATTTCACGATGATCAACATGGAACTGCTGTAGTAACTTTAGCGGGTCTAATTAACGCATTAAA
GTTAGTGGGCAAAAAATTCAACGAGATAAAAGTTGCTATAAGTGGAGCTGGAGCTGCTGGAATCGCAATAGCAAAGTTGC
TCCATCATGTTGGGGTTAAAGAAATATTGCTTGTTGATAGAGCTGGAATAATCTATGAAGGGAGAAAAGAAAACATGAAT
CCTTACAAAGAGGAAGTCGCAAGGTTCAACATTCATGGGGTTCAAGGAGATTTGGCAAAAGCTATGGAGGGTGCTGATGT
TTTTATTGGAGTAAGCGTAGGAGGAATAGTAAAACCCTGGATGATAAAGAAAATGGCCGATGATCCCATAGTGTTTGCAA
TGGCGAATCCAATTCCAGAAATTATGCCAGAAGATGCGAAAAAGGCTGGGGCAAAAATAGTGGCCACGGGGAGGAGTGAC
TATCCAAACCAAATAAACAATGTCTTGGGCTTTCCTGGGATATTTAGGGGGGCACTAGATGTTAGGGCTACCGATATAAC
TTTAAATATGAACATTGCAGCGGCTGAGGCAATAGCATCTGTTGTGAGCGATGAAGAACTTAGTGAGGACTACATAATAC
CCTCTCCACTACATCCCGACGTTTATCCAAAAGAAGCCAGAGCAGTTGCAGAACAAGCAATTAGGGATGGTGTAGCTAGA
AGAAAGCTATCTGGGGAGTGGGTAGAAGAGCATACTAGGAAGCTCCGTGAATTTTATGCCGCACTCATTGAGCCCTTAAA
TGAAAGAAGGAAACAGTACATTACTTAA

Upstream 100 bases:

>100_bases
GATATTGATGAAAAGCATAGGATTGGAAGAGATATATACGTGATTAGATACTAGCAATGCTTTTATCCTACCTGGCTCAT
TGTTCCTGGGTGAAAGTGAT

Downstream 100 bases:

>100_bases
ACTCTTTCTAACCTTGTGAAGTCTATTTTATATTCAGAAAATTTTATATTTTTTGAAATCCTTATTTATCACCTGGGGGG
ATATATAGTGCATGAGCTTC

Product: malate oxidoreductase (malic enzyme)

Products: NA

Alternate protein names: NAD-ME 4 [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MARITEEQRRKLAQDALEYHRNNFPGNGKIEVIPKVPLRDFYDLSLAYTPGVAEPCKVIAKKGNYEDYTIIPNTVAVVTD
GSAILGLGNIGVLAGMPVMEGKCVLFKALAGIDAFPILIDTQDVEKIVETIKLISKGFGGINLEDISAPRCFEIEMRLKR
ELDIPVFHDDQHGTAVVTLAGLINALKLVGKKFNEIKVAISGAGAAGIAIAKLLHHVGVKEILLVDRAGIIYEGRKENMN
PYKEEVARFNIHGVQGDLAKAMEGADVFIGVSVGGIVKPWMIKKMADDPIVFAMANPIPEIMPEDAKKAGAKIVATGRSD
YPNQINNVLGFPGIFRGALDVRATDITLNMNIAAAEAIASVVSDEELSEDYIIPSPLHPDVYPKEARAVAEQAIRDGVAR
RKLSGEWVEEHTRKLREFYAALIEPLNERRKQYIT

Sequences:

>Translated_435_residues
MARITEEQRRKLAQDALEYHRNNFPGNGKIEVIPKVPLRDFYDLSLAYTPGVAEPCKVIAKKGNYEDYTIIPNTVAVVTD
GSAILGLGNIGVLAGMPVMEGKCVLFKALAGIDAFPILIDTQDVEKIVETIKLISKGFGGINLEDISAPRCFEIEMRLKR
ELDIPVFHDDQHGTAVVTLAGLINALKLVGKKFNEIKVAISGAGAAGIAIAKLLHHVGVKEILLVDRAGIIYEGRKENMN
PYKEEVARFNIHGVQGDLAKAMEGADVFIGVSVGGIVKPWMIKKMADDPIVFAMANPIPEIMPEDAKKAGAKIVATGRSD
YPNQINNVLGFPGIFRGALDVRATDITLNMNIAAAEAIASVVSDEELSEDYIIPSPLHPDVYPKEARAVAEQAIRDGVAR
RKLSGEWVEEHTRKLREFYAALIEPLNERRKQYIT
>Mature_434_residues
ARITEEQRRKLAQDALEYHRNNFPGNGKIEVIPKVPLRDFYDLSLAYTPGVAEPCKVIAKKGNYEDYTIIPNTVAVVTDG
SAILGLGNIGVLAGMPVMEGKCVLFKALAGIDAFPILIDTQDVEKIVETIKLISKGFGGINLEDISAPRCFEIEMRLKRE
LDIPVFHDDQHGTAVVTLAGLINALKLVGKKFNEIKVAISGAGAAGIAIAKLLHHVGVKEILLVDRAGIIYEGRKENMNP
YKEEVARFNIHGVQGDLAKAMEGADVFIGVSVGGIVKPWMIKKMADDPIVFAMANPIPEIMPEDAKKAGAKIVATGRSDY
PNQINNVLGFPGIFRGALDVRATDITLNMNIAAAEAIASVVSDEELSEDYIIPSPLHPDVYPKEARAVAEQAIRDGVARR
KLSGEWVEEHTRKLREFYAALIEPLNERRKQYIT

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=386, Percent_Identity=29.5336787564767, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI4505145, Length=363, Percent_Identity=30.0275482093664, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI62420882, Length=357, Percent_Identity=26.890756302521, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI62420880, Length=357, Percent_Identity=26.890756302521, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI239049447, Length=357, Percent_Identity=26.890756302521, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI270265879, Length=322, Percent_Identity=30.7453416149068, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI1788806, Length=426, Percent_Identity=46.9483568075117, Blast_Score=350, Evalue=1e-97,
Organism=Escherichia coli, GI87081919, Length=397, Percent_Identity=27.7078085642317, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17537199, Length=450, Percent_Identity=25.7777777777778, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6322823, Length=388, Percent_Identity=27.319587628866, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281362674, Length=365, Percent_Identity=29.5890410958904, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21356279, Length=365, Percent_Identity=29.5890410958904, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI281362672, Length=365, Percent_Identity=29.5890410958904, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24646388, Length=439, Percent_Identity=29.8405466970387, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24646386, Length=439, Percent_Identity=29.8405466970387, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI78707242, Length=399, Percent_Identity=28.0701754385965, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI78707238, Length=399, Percent_Identity=28.0701754385965, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI78707240, Length=358, Percent_Identity=27.3743016759777, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI281363505, Length=360, Percent_Identity=26.9444444444444, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI78707236, Length=360, Percent_Identity=26.9444444444444, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI281363503, Length=360, Percent_Identity=26.9444444444444, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI78707232, Length=360, Percent_Identity=26.9444444444444, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 47571; Mature: 47440

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARITEEQRRKLAQDALEYHRNNFPGNGKIEVIPKVPLRDFYDLSLAYTPGVAEPCKVIA
CCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHCCEEEECCCCCHHHHHHH
KKGNYEDYTIIPNTVAVVTDGSAILGLGNIGVLAGMPVMEGKCVLFKALAGIDAFPILID
HCCCCCCEEECCCEEEEEECCCEEEEECCHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEE
TQDVEKIVETIKLISKGFGGINLEDISAPRCFEIEMRLKRELDIPVFHDDQHGTAVVTLA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHCCCCCEEECCCCCCHHHHHH
GLINALKLVGKKFNEIKVAISGAGAAGIAIAKLLHHVGVKEILLVDRAGIIYEGRKENMN
HHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCHHHEEEEECCCEEECCCCCCCC
PYKEEVARFNIHGVQGDLAKAMEGADVFIGVSVGGIVKPWMIKKMADDPIVFAMANPIPE
HHHHHHHHEEECCCCHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEECCCCHH
IMPEDAKKAGAKIVATGRSDYPNQINNVLGFPGIFRGALDVRATDITLNMNIAAAEAIAS
CCCCHHHHCCCEEEEECCCCCCHHHHHHCCCCHHHHCCCEEEEEEEEEECCHHHHHHHHH
VVSDEELSEDYIIPSPLHPDVYPKEARAVAEQAIRDGVARRKLSGEWVEEHTRKLREFYA
HHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ALIEPLNERRKQYIT
HHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARITEEQRRKLAQDALEYHRNNFPGNGKIEVIPKVPLRDFYDLSLAYTPGVAEPCKVIA
CCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHCCEEEECCCCCHHHHHHH
KKGNYEDYTIIPNTVAVVTDGSAILGLGNIGVLAGMPVMEGKCVLFKALAGIDAFPILID
HCCCCCCEEECCCEEEEEECCCEEEEECCHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEE
TQDVEKIVETIKLISKGFGGINLEDISAPRCFEIEMRLKRELDIPVFHDDQHGTAVVTLA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHCCCCCEEECCCCCCHHHHHH
GLINALKLVGKKFNEIKVAISGAGAAGIAIAKLLHHVGVKEILLVDRAGIIYEGRKENMN
HHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCHHHEEEEECCCEEECCCCCCCC
PYKEEVARFNIHGVQGDLAKAMEGADVFIGVSVGGIVKPWMIKKMADDPIVFAMANPIPE
HHHHHHHHEEECCCCHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEECCCCHH
IMPEDAKKAGAKIVATGRSDYPNQINNVLGFPGIFRGALDVRATDITLNMNIAAAEAIAS
CCCCHHHHCCCEEEEECCCCCCHHHHHHCCCCHHHHCCCEEEEEEEEEECCHHHHHHHHH
VVSDEELSEDYIIPSPLHPDVYPKEARAVAEQAIRDGVARRKLSGEWVEEHTRKLREFYA
HHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ALIEPLNERRKQYIT
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]