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The map label for this gene is nfi

Identifier: 18977359

GI number: 18977359

Start: 941348

End: 941923

Strand: Direct

Name: nfi

Synonym: PF0987

Alternate gene names: 18977359

Gene position: 941348-941923 (Clockwise)

Preceding gene: 18977357

Following gene: 18977360

Centisome position: 49.33

GC content: 38.54

Gene sequence:

>576_bases
ATGATAGATCTTAGAAAACTGACGGAAGTGCAGCGAAGGCTTAGTAGGAAAATAGTGGAAAAACCAATAGATATAGCGAA
AGTGAAGAGAGTTGGAGCCGTGGACGTCTCTTATAAGAATAACTTTGCTAAAGCTGCATTTGTTTGCGTAGAATTTCCTT
CTGGTGAGATAATAAAAACAAAAACAATAGTAACAACCGTTGAGTTTCCCTATATCCCCACGTTTTTCTTTTTAAGGGAG
ACAAAACCAATATTGTTGGCTGTTAAAGACGAGAACTTTGACGTTCTGTTAGTTGAAGGGCATGGAAAAGCTCATCCCAG
GAGATATGGACTAGCATCTCACATAGGAGTAATTCTTTCAAAGCCAACAATAGGAGTTGCCAAAAGGTTATTGAGAGGCG
TGTCAAAAGATACCTACGTAAAGGTGGGAAAAGCTTTTGTTAGTGTAGGCAACCTAATAACTCTCAATGATGCTGTCAGA
ATTGTAGAAAAATTGCTAGATGAAAACGGTTATCCAAAGCCCTTAAATATAGCCGACAAACTCTCCAAAAGAGGTGAGTC
TCGTGAAAAAGAGTAA

Upstream 100 bases:

>100_bases
AAGCAAGATCAGGCGAGGTACACCTTGGAAAGAACTCTTGAAGATCTAACAAGAGCAAAAGTTAACAGAAGGGTTGAAGA
AAAACTGGAGGCTTTTTTGA

Downstream 100 bases:

>100_bases
CCTTGATTTGTTAATCCTCCTTGCAAAGGCAGGGGGGATAGAGAAAGAGATTCTAACGACTTCTAGAGAACTTAGTAAAA
TGTTAAACGTTTCTCCTCAA

Product: hypothetical protein

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MIDLRKLTEVQRRLSRKIVEKPIDIAKVKRVGAVDVSYKNNFAKAAFVCVEFPSGEIIKTKTIVTTVEFPYIPTFFFLRE
TKPILLAVKDENFDVLLVEGHGKAHPRRYGLASHIGVILSKPTIGVAKRLLRGVSKDTYVKVGKAFVSVGNLITLNDAVR
IVEKLLDENGYPKPLNIADKLSKRGESREKE

Sequences:

>Translated_191_residues
MIDLRKLTEVQRRLSRKIVEKPIDIAKVKRVGAVDVSYKNNFAKAAFVCVEFPSGEIIKTKTIVTTVEFPYIPTFFFLRE
TKPILLAVKDENFDVLLVEGHGKAHPRRYGLASHIGVILSKPTIGVAKRLLRGVSKDTYVKVGKAFVSVGNLITLNDAVR
IVEKLLDENGYPKPLNIADKLSKRGESREKE
>Mature_191_residues
MIDLRKLTEVQRRLSRKIVEKPIDIAKVKRVGAVDVSYKNNFAKAAFVCVEFPSGEIIKTKTIVTTVEFPYIPTFFFLRE
TKPILLAVKDENFDVLLVEGHGKAHPRRYGLASHIGVILSKPTIGVAKRLLRGVSKDTYVKVGKAFVSVGNLITLNDAVR
IVEKLLDENGYPKPLNIADKLSKRGESREKE

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family

Homologues:

Organism=Homo sapiens, GI31542775, Length=226, Percent_Identity=28.3185840707965, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI87082357, Length=211, Percent_Identity=29.3838862559242, Blast_Score=91, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NFI_PYRFU (Q8U263)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_578716.1
- ProteinModelPortal:   Q8U263
- EnsemblBacteria:   EBPYRT00000004058
- GeneID:   1468852
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF0987
- NMPDR:   fig|186497.1.peg.1023
- GeneTree:   EBGT00050000022947
- HOGENOM:   HBG716360
- OMA:   VEGHGKA
- ProtClustDB:   CLSK253494
- BRENDA:   3.1.21.7
- GO:   GO:0005737
- HAMAP:   MF_00801
- InterPro:   IPR007581

Pfam domain/function: PF04493 Endonuclease_5

EC number: =3.1.21.7

Molecular weight: Translated: 21417; Mature: 21417

Theoretical pI: Translated: 10.50; Mature: 10.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLRKLTEVQRRLSRKIVEKPIDIAKVKRVGAVDVSYKNNFAKAAFVCVEFPSGEIIKT
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCEEEEECCCCCCEEEEEEEECCCCCEEEE
KTIVTTVEFPYIPTFFFLRETKPILLAVKDENFDVLLVEGHGKAHPRRYGLASHIGVILS
EEEEEEEECCCCCCEEEEECCCCEEEEEECCCEEEEEEECCCCCCCHHHCHHHHHHHHCC
KPTIGVAKRLLRGVSKDTYVKVGKAFVSVGNLITLNDAVRIVEKLLDENGYPKPLNIADK
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEHHHHHHHHHHHCCCCCCCCCCHHHH
LSKRGESREKE
HHHCCCCCCCC
>Mature Secondary Structure
MIDLRKLTEVQRRLSRKIVEKPIDIAKVKRVGAVDVSYKNNFAKAAFVCVEFPSGEIIKT
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCEEEEECCCCCCEEEEEEEECCCCCEEEE
KTIVTTVEFPYIPTFFFLRETKPILLAVKDENFDVLLVEGHGKAHPRRYGLASHIGVILS
EEEEEEEECCCCCCEEEEECCCCEEEEEECCCEEEEEEECCCCCCCHHHCHHHHHHHHCC
KPTIGVAKRLLRGVSKDTYVKVGKAFVSVGNLITLNDAVRIVEKLLDENGYPKPLNIADK
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEHHHHHHHHHHHCCCCCCCCCCHHHH
LSKRGESREKE
HHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA