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The map label for this gene is leuA

Identifier: 18977309

GI number: 18977309

Start: 901054

End: 902553

Strand: Direct

Name: leuA

Synonym: PF0937

Alternate gene names: 18977309

Gene position: 901054-902553 (Clockwise)

Preceding gene: 18977308

Following gene: 18977310

Centisome position: 47.22

GC content: 44.13

Gene sequence:

>1500_bases
ATGGCGAGGAAGATAAAGATTTTCGACACTACACTAAGGGATGGAGAGCAGACCCCAGGAGTTAGCTTAACAGTAGAAGA
AAAGATAGAGATAGCAAAGCAACTGGCAAGGCTTAATGTAGATGTCATTGAGGCAGGATTCCCAATATCTTCTCCTGGGG
AATTTGAGGCTGTAAAGAGAATAGCTAGGGAGGTTAGAGGCCCAACAATTGCGGCTTTAGCTAGGGCCGTAAAGAAGGAT
ATAGATGCCGCAGGAGAAGCCCTAAAGGATGCAGAAAGCAAGAGAATACATACATTCATAGCAACCTCACTAATTCACAT
GAAGTATAAGCTGAGGAAAACTCCAGAAGAAGTTAAAAAGATGGCCGTAGAGGCCGTGGAGTATGCCACTAAGTACACGG
ATGACATTGAATTTTCTGCAGAAGATGCCACGAGAAGTGACTGGAACTTTTTGGTTGAGGTCTACGAGGCAGTCATAGAT
GCGGGAGCAACAACAATTAACGTTCCCGATACCGTTGGTTATACAACTCCTGAAGAATTTTATGAGTTGATTAGGTATCT
CAAGCGCAACATAACTAATCTAAATGGCGTAACGATAAGCGTTCACTGTCACAACGATCTAGGCTTAGCGGTTGCAAACT
CCCTCTCAGCAGTGAGGGCTGGAGCTGATCAGGTTGAAGTCACCGTTAATGGAATTGGAGAGAGAGCTGGAAATGCTGCA
TTAGAAGAAGTTGTCGTGGCATTAGACGTGAGGAAAGACTTCTACAATGTTGAGACGGGCATAAATCTAGGGGAGATAGC
CAGGACAAGCAAGCTAGTGGCGAGGCTAACTGGAATTGAAGTTCCACCAAATAAAGCAGTGGTAGGAGCAAATGCCTTTG
CCCATGAATCTGGAATTCATCAGGATGGGGTTCTAAAGGAAAGAACGACTTATGAGATAATAGACCCCAGGAAGCTGGGG
TTCTCGGGAAGTAAAATAGTTTTAGGTAAACACTCTGGAAGGCACGCATTTAGGAAAAAGCTCGAGGAGATGGGATATAG
ACTTAGTGAAGAGGAGATAAATAGGTTGTTTGCAAAATTCAAGGAGATAGCCGACAGGAAGAAGGGCTTAACTGAGCTCG
ATATTGAAGCAATAGTCCAGGAAGAGCTAGGGAAAGGGAAAGGCAAGTATAGCGTTGAGGTTCTCCACGTAATTTCTGGG
AAGATTTCCACAGCCACGGTGAGGGTTCAAGGGGATGGTATTGATAAAATTGAGAGTGCTTGGTCGAGCAATGGCCCCAT
AGATGCACTCTTTGCTGCTATAAATAGAGCCCTTGGAATTGATTGCAAATTAAAGGAGTACAGAGTTTCTTCTGTTACCT
CTGGAAGAGATGCCTTGGGAGAAGTTTTAGTTAGAGTTGAATATAACGGAGAGATTTACGTTGGAAGAGGTTTGAGCACC
GACATTATTGAGGCAAGTGCTCAGGCGTATCTTTCTGCTCTAAATAGAATTAGGAGGTGA

Upstream 100 bases:

>100_bases
ATCGAGGAGCACGAGATCGAAAAAGTTGGAAGGTTCATTAGGAGAATGTCAGGCCTTGAAAAGTGATTTCTCCCCTTTCC
ACTTTTTTGGAGGGAAAGTG

Downstream 100 bases:

>100_bases
AATTATGGGCGGAATGACAATAGCTGAAAAAATTTTGGCAACTCATGCCGGAAAGGAAGAGGTAAAACCTGGAGAGATAG
TTTTGGCAAAAGTGGACTTC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MARKIKIFDTTLRDGEQTPGVSLTVEEKIEIAKQLARLNVDVIEAGFPISSPGEFEAVKRIAREVRGPTIAALARAVKKD
IDAAGEALKDAESKRIHTFIATSLIHMKYKLRKTPEEVKKMAVEAVEYATKYTDDIEFSAEDATRSDWNFLVEVYEAVID
AGATTINVPDTVGYTTPEEFYELIRYLKRNITNLNGVTISVHCHNDLGLAVANSLSAVRAGADQVEVTVNGIGERAGNAA
LEEVVVALDVRKDFYNVETGINLGEIARTSKLVARLTGIEVPPNKAVVGANAFAHESGIHQDGVLKERTTYEIIDPRKLG
FSGSKIVLGKHSGRHAFRKKLEEMGYRLSEEEINRLFAKFKEIADRKKGLTELDIEAIVQEELGKGKGKYSVEVLHVISG
KISTATVRVQGDGIDKIESAWSSNGPIDALFAAINRALGIDCKLKEYRVSSVTSGRDALGEVLVRVEYNGEIYVGRGLST
DIIEASAQAYLSALNRIRR

Sequences:

>Translated_499_residues
MARKIKIFDTTLRDGEQTPGVSLTVEEKIEIAKQLARLNVDVIEAGFPISSPGEFEAVKRIAREVRGPTIAALARAVKKD
IDAAGEALKDAESKRIHTFIATSLIHMKYKLRKTPEEVKKMAVEAVEYATKYTDDIEFSAEDATRSDWNFLVEVYEAVID
AGATTINVPDTVGYTTPEEFYELIRYLKRNITNLNGVTISVHCHNDLGLAVANSLSAVRAGADQVEVTVNGIGERAGNAA
LEEVVVALDVRKDFYNVETGINLGEIARTSKLVARLTGIEVPPNKAVVGANAFAHESGIHQDGVLKERTTYEIIDPRKLG
FSGSKIVLGKHSGRHAFRKKLEEMGYRLSEEEINRLFAKFKEIADRKKGLTELDIEAIVQEELGKGKGKYSVEVLHVISG
KISTATVRVQGDGIDKIESAWSSNGPIDALFAAINRALGIDCKLKEYRVSSVTSGRDALGEVLVRVEYNGEIYVGRGLST
DIIEASAQAYLSALNRIRR
>Mature_498_residues
ARKIKIFDTTLRDGEQTPGVSLTVEEKIEIAKQLARLNVDVIEAGFPISSPGEFEAVKRIAREVRGPTIAALARAVKKDI
DAAGEALKDAESKRIHTFIATSLIHMKYKLRKTPEEVKKMAVEAVEYATKYTDDIEFSAEDATRSDWNFLVEVYEAVIDA
GATTINVPDTVGYTTPEEFYELIRYLKRNITNLNGVTISVHCHNDLGLAVANSLSAVRAGADQVEVTVNGIGERAGNAAL
EEVVVALDVRKDFYNVETGINLGEIARTSKLVARLTGIEVPPNKAVVGANAFAHESGIHQDGVLKERTTYEIIDPRKLGF
SGSKIVLGKHSGRHAFRKKLEEMGYRLSEEEINRLFAKFKEIADRKKGLTELDIEAIVQEELGKGKGKYSVEVLHVISGK
ISTATVRVQGDGIDKIESAWSSNGPIDALFAAINRALGIDCKLKEYRVSSVTSGRDALGEVLVRVEYNGEIYVGRGLSTD
IIEASAQAYLSALNRIRR

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=506, Percent_Identity=50, Blast_Score=476, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6320071, Length=379, Percent_Identity=31.1345646437995, Blast_Score=178, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6320019, Length=376, Percent_Identity=31.3829787234043, Blast_Score=178, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6324682, Length=559, Percent_Identity=27.0125223613596, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6324225, Length=563, Percent_Identity=26.9982238010657, Blast_Score=145, Evalue=2e-35,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_PYRFU (Q8U2A2)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_578666.1
- ProteinModelPortal:   Q8U2A2
- SMR:   Q8U2A2
- EnsemblBacteria:   EBPYRT00000005506
- GeneID:   1468801
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF0937
- NMPDR:   fig|186497.1.peg.972
- GeneTree:   EBGT00050000022307
- HOGENOM:   HBG701974
- OMA:   ENSFAHE
- ProtClustDB:   PRK00915
- BRENDA:   2.3.3.13
- HAMAP:   MF_01025_A
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 54719; Mature: 54588

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKIKIFDTTLRDGEQTPGVSLTVEEKIEIAKQLARLNVDVIEAGFPISSPGEFEAVKR
CCCEEEEEECHHCCCCCCCCEEEEHHHHHHHHHHHHHCCHHEEECCCCCCCCCHHHHHHH
IAREVRGPTIAALARAVKKDIDAAGEALKDAESKRIHTFIATSLIHMKYKLRKTPEEVKK
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
MAVEAVEYATKYTDDIEFSAEDATRSDWNFLVEVYEAVIDAGATTINVPDTVGYTTPEEF
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHH
YELIRYLKRNITNLNGVTISVHCHNDLGLAVANSLSAVRAGADQVEVTVNGIGERAGNAA
HHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHH
LEEVVVALDVRKDFYNVETGINLGEIARTSKLVARLTGIEVPPNKAVVGANAFAHESGIH
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHCCCCC
QDGVLKERTTYEIIDPRKLGFSGSKIVLGKHSGRHAFRKKLEEMGYRLSEEEINRLFAKF
CCCCCCCCCCEEEECCHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHH
KEIADRKKGLTELDIEAIVQEELGKGKGKYSVEVLHVISGKISTATVRVQGDGIDKIESA
HHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEEEEEECCCCHHHHHH
WSSNGPIDALFAAINRALGIDCKLKEYRVSSVTSGRDALGEVLVRVEYNGEIYVGRGLST
HCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHCCCCHHHHHHEEEEEEECCEEEECCCCCH
DIIEASAQAYLSALNRIRR
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARKIKIFDTTLRDGEQTPGVSLTVEEKIEIAKQLARLNVDVIEAGFPISSPGEFEAVKR
CCEEEEEECHHCCCCCCCCEEEEHHHHHHHHHHHHHCCHHEEECCCCCCCCCHHHHHHH
IAREVRGPTIAALARAVKKDIDAAGEALKDAESKRIHTFIATSLIHMKYKLRKTPEEVKK
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
MAVEAVEYATKYTDDIEFSAEDATRSDWNFLVEVYEAVIDAGATTINVPDTVGYTTPEEF
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHH
YELIRYLKRNITNLNGVTISVHCHNDLGLAVANSLSAVRAGADQVEVTVNGIGERAGNAA
HHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHH
LEEVVVALDVRKDFYNVETGINLGEIARTSKLVARLTGIEVPPNKAVVGANAFAHESGIH
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHCCCCC
QDGVLKERTTYEIIDPRKLGFSGSKIVLGKHSGRHAFRKKLEEMGYRLSEEEINRLFAKF
CCCCCCCCCCEEEECCHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHH
KEIADRKKGLTELDIEAIVQEELGKGKGKYSVEVLHVISGKISTATVRVQGDGIDKIESA
HHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEEEEEECCCCHHHHHH
WSSNGPIDALFAAINRALGIDCKLKEYRVSSVTSGRDALGEVLVRVEYNGEIYVGRGLST
HCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHCCCCHHHHHHEEEEEEECCEEEECCCCCH
DIIEASAQAYLSALNRIRR
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA