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The map label for this gene is pyrI [H]

Identifier: 18976970

GI number: 18976970

Start: 619025

End: 619483

Strand: Reverse

Name: pyrI [H]

Synonym: PF0598

Alternate gene names: 18976970

Gene position: 619483-619025 (Counterclockwise)

Preceding gene: 18976971

Following gene: 18976968

Centisome position: 32.46

GC content: 42.48

Gene sequence:

>459_bases
ATGGCAGAGCTTAAGGTTTCCGCAATCAAGGAGGGAACGGTAATAGACCACATACCAGCGGGCAAAGGCCTTAAGGTTAT
CCAAATCCTCGGGCTTGGAGAGTTGAAGAATGGAGGAGCGGTTTTATTAGCTATGAACGTACCAAGCAAAAAGCTGGGAA
GAAAGGATATAGTTAAGGTTGAAGGCAAGTTCCTGAGTGAGGAAGAGGTCAACAAAATAGCGCTAGTTGCTCCAACTGCA
ACCGTTAACATAATCAGAGAGTACAAGGTTGTTGAGAAGTTTAAGGTTGAGATTCCAGATGTTATTGAGGGAATATTAAA
GTGTGGCAATCCCAACTGTATAACCCATTACGAATATGTAACTCCAAAGTTCTACGTGATCAGCAAGGAACCATTGAAAG
TTAGGTGTCACTACTGTGAAAGAACAATGGAAGAAGAGGAGATACTGGCTAACCTCTAA

Upstream 100 bases:

>100_bases
AGAAGTTGATAAAACCAAGCACGCGATCTACTTTAAGCAAGTGTTTAACGGAATTCCCGTAAGAATGGCCCTCTTGGGCC
TAGTCTTGGGGGTGATTTGA

Downstream 100 bases:

>100_bases
AGTTGAAGGCCAAGGCAACTTCCAGTGCAGTTCCCAGGGGAAGAATGTCCTCATCGACGTCAAACCTTGGGTTGTGGTGT
GGGTAGATTATCCCCTTCTT

Product: aspartate carbamoyltransferase regulatory subunit

Products: phosphate; N-carbamoyl-L-aspartate

Alternate protein names: NA

Number of amino acids: Translated: 152; Mature: 151

Protein sequence:

>152_residues
MAELKVSAIKEGTVIDHIPAGKGLKVIQILGLGELKNGGAVLLAMNVPSKKLGRKDIVKVEGKFLSEEEVNKIALVAPTA
TVNIIREYKVVEKFKVEIPDVIEGILKCGNPNCITHYEYVTPKFYVISKEPLKVRCHYCERTMEEEEILANL

Sequences:

>Translated_152_residues
MAELKVSAIKEGTVIDHIPAGKGLKVIQILGLGELKNGGAVLLAMNVPSKKLGRKDIVKVEGKFLSEEEVNKIALVAPTA
TVNIIREYKVVEKFKVEIPDVIEGILKCGNPNCITHYEYVTPKFYVISKEPLKVRCHYCERTMEEEEILANL
>Mature_151_residues
AELKVSAIKEGTVIDHIPAGKGLKVIQILGLGELKNGGAVLLAMNVPSKKLGRKDIVKVEGKFLSEEEVNKIALVAPTAT
VNIIREYKVVEKFKVEIPDVIEGILKCGNPNCITHYEYVTPKFYVISKEPLKVRCHYCERTMEEEEILANL

Specific function: Involved in allosteric regulation of aspartate carbamoyltransferase [H]

COG id: COG1781

COG function: function code F; Aspartate carbamoyltransferase, regulatory subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrI family [H]

Homologues:

Organism=Escherichia coli, GI1790692, Length=150, Percent_Identity=43.3333333333333, Blast_Score=131, Evalue=2e-32,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020545
- InterPro:   IPR002801
- InterPro:   IPR020542
- ProDom:   PD006194 [H]

Pfam domain/function: PF01948 PyrI; PF02748 PyrI_C [H]

EC number: 2.1.3.2

Molecular weight: Translated: 16890; Mature: 16759

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELKVSAIKEGTVIDHIPAGKGLKVIQILGLGELKNGGAVLLAMNVPSKKLGRKDIVKV
CCCCEEEEECCCCEEEECCCCCCCEEEEEEECCEECCCCEEEEEEECCHHHCCCCCEEEE
EGKFLSEEEVNKIALVAPTATVNIIREYKVVEKFKVEIPDVIEGILKCGNPNCITHYEYV
CCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEEC
TPKFYVISKEPLKVRCHYCERTMEEEEILANL
CCEEEEEECCCCEEEEHHHHCCCCHHHHHHCC
>Mature Secondary Structure 
AELKVSAIKEGTVIDHIPAGKGLKVIQILGLGELKNGGAVLLAMNVPSKKLGRKDIVKV
CCCEEEEECCCCEEEECCCCCCCEEEEEEECCEECCCCEEEEEEECCHHHCCCCCEEEE
EGKFLSEEEVNKIALVAPTATVNIIREYKVVEKFKVEIPDVIEGILKCGNPNCITHYEYV
CCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEEC
TPKFYVISKEPLKVRCHYCERTMEEEEILANL
CCEEEEEECCCCEEEEHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: carbamoyl phosphate; L-aspartate

Specific reaction: carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9209027; 12622808 [H]