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The map label for this gene is purK [H]

Identifier: 18976798

GI number: 18976798

Start: 433790

End: 434908

Strand: Direct

Name: purK [H]

Synonym: PF0426

Alternate gene names: 18976798

Gene position: 433790-434908 (Clockwise)

Preceding gene: 18976797

Following gene: 18976799

Centisome position: 22.73

GC content: 40.48

Gene sequence:

>1119_bases
ATGATAGTGGGAATTCTTGGAGGGGGTCAATTAGCTAAGATGATGGCTCAGGAAGCCAAGAAAATGGGCTTTAAAGTAGC
TATCCTTGATCCTCAAGAAGAACCTCCAGCTAAAGGAGTTTCCGATTATCATATTCTAGGTAGCTTTAAAGACGAGAAAG
CTATAAGAAAATTAGCAGAGATATCTGATGTTCTAACGTACGATATAGAGCACATAAATGTTCAAGCTCTAAAAGAGCTT
GAGAAGGAAGGTGTGCAAATATATCCATCACCTAGGGTTCTGGAAATAATTCAGGATAAACTAGTACAAATGCAGGTTAT
GAAGGAGGCTGGAATTCCTGTCCCACGCTTTATAAAAGCAGATAAAGACGAGTTGGTTGACAAGGCCTTAGAATTTGGAT
TTCCTTTAGTTCAAAAGACTAGAAGAGAAGGATATGATGGAAAAGGAGTAGCAGTTATAAGAGACAAAAGTGAGCTAGCT
AAATTGATTCCTGCAGACTCAATGCTTCAGGAGTTCGTAAACATAGAAAAAGAGATTGCCGTAATGGTGGCTAGAGATCA
GGAGGGGAATACTGTAGCATATCCTGTTGTTGAGATGACGTTTAACGAGGCAAATATACTCGATATGCTCATTGCACCCG
CAAGGATTGAGGAGGAAATAGCTGAAGAAGCAAAGAGAATTGCAATAAAGGCTGTAAAAGCTTTAGATGGGGTGGGAATA
TTTGGAGTAGAGATGTTTCTGACAAAAGATGGGGAGGTGTATCTTAACGAAATAGCTCCAAGGCCCCATAATTCAGGTCA
TTACACTATAGAGGCTTGCCTAACAAGCCAGTTTGAACAGCATATAAGAGCTATAACAGGTCTCCCGCTGGGAAGTTCTG
AGCTTTTAATTCCCGCGGTAATGTTTAACCTCCTTGGAGAAGGAAACGGGAAGCCAAAGGTTATTGGAATGAAGGAAGCT
CTTAAATTCCCAGGAGTGTATGTCCATATTTATGGAAAGTCCGTTGTTAAGCCTCTAAGAAAGATGGGTCATGTAACCGT
CGTAAATAGAAATTTGGAAAAGGCCTTAGAGATTGCCCACAAAGTTAAGAAGGTAATAAAGGTGGTTGGAGATGCCTAA

Upstream 100 bases:

>100_bases
TTCAAAGGAGATAAAATAGTTGGACGGAGAATTTGTTGACAAACTTCTGTAAATTTTTATACCCAAAACTATTTAAAATC
TCCATATATGTGTAAATTTG

Downstream 100 bases:

>100_bases
AGTTGGAATCATAATGGGTAGTGATTCAGATTTGCCCGTAATGAAGGAAGCAGCTAAAGTTCTGGAAGAGTTTGGAGTAG
ATTATGAAATAACAATAGTC

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MIVGILGGGQLAKMMAQEAKKMGFKVAILDPQEEPPAKGVSDYHILGSFKDEKAIRKLAEISDVLTYDIEHINVQALKEL
EKEGVQIYPSPRVLEIIQDKLVQMQVMKEAGIPVPRFIKADKDELVDKALEFGFPLVQKTRREGYDGKGVAVIRDKSELA
KLIPADSMLQEFVNIEKEIAVMVARDQEGNTVAYPVVEMTFNEANILDMLIAPARIEEEIAEEAKRIAIKAVKALDGVGI
FGVEMFLTKDGEVYLNEIAPRPHNSGHYTIEACLTSQFEQHIRAITGLPLGSSELLIPAVMFNLLGEGNGKPKVIGMKEA
LKFPGVYVHIYGKSVVKPLRKMGHVTVVNRNLEKALEIAHKVKKVIKVVGDA

Sequences:

>Translated_372_residues
MIVGILGGGQLAKMMAQEAKKMGFKVAILDPQEEPPAKGVSDYHILGSFKDEKAIRKLAEISDVLTYDIEHINVQALKEL
EKEGVQIYPSPRVLEIIQDKLVQMQVMKEAGIPVPRFIKADKDELVDKALEFGFPLVQKTRREGYDGKGVAVIRDKSELA
KLIPADSMLQEFVNIEKEIAVMVARDQEGNTVAYPVVEMTFNEANILDMLIAPARIEEEIAEEAKRIAIKAVKALDGVGI
FGVEMFLTKDGEVYLNEIAPRPHNSGHYTIEACLTSQFEQHIRAITGLPLGSSELLIPAVMFNLLGEGNGKPKVIGMKEA
LKFPGVYVHIYGKSVVKPLRKMGHVTVVNRNLEKALEIAHKVKKVIKVVGDA
>Mature_372_residues
MIVGILGGGQLAKMMAQEAKKMGFKVAILDPQEEPPAKGVSDYHILGSFKDEKAIRKLAEISDVLTYDIEHINVQALKEL
EKEGVQIYPSPRVLEIIQDKLVQMQVMKEAGIPVPRFIKADKDELVDKALEFGFPLVQKTRREGYDGKGVAVIRDKSELA
KLIPADSMLQEFVNIEKEIAVMVARDQEGNTVAYPVVEMTFNEANILDMLIAPARIEEEIAEEAKRIAIKAVKALDGVGI
FGVEMFLTKDGEVYLNEIAPRPHNSGHYTIEACLTSQFEQHIRAITGLPLGSSELLIPAVMFNLLGEGNGKPKVIGMKEA
LKFPGVYVHIYGKSVVKPLRKMGHVTVVNRNLEKALEIAHKVKKVIKVVGDA

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=356, Percent_Identity=29.4943820224719, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1788155, Length=382, Percent_Identity=25.9162303664921, Blast_Score=114, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6324702, Length=370, Percent_Identity=41.3513513513514, Blast_Score=272, Evalue=5e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006115
- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp; PF03446 NAD_binding_2 [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 41100; Mature: 41100

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVGILGGGQLAKMMAQEAKKMGFKVAILDPQEEPPAKGVSDYHILGSFKDEKAIRKLAE
CEEEEECCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHH
ISDVLTYDIEHINVQALKELEKEGVQIYPSPRVLEIIQDKLVQMQVMKEAGIPVPRFIKA
HHHHHHHHHHHCCHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHCC
DKDELVDKALEFGFPLVQKTRREGYDGKGVAVIRDKSELAKLIPADSMLQEFVNIEKEIA
CHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEECHHHHHHHCCHHHHHHHHHCCHHHEE
VMVARDQEGNTVAYPVVEMTFNEANILDMLIAPARIEEEIAEEAKRIAIKAVKALDGVGI
EEEEECCCCCEEEEEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
FGVEMFLTKDGEVYLNEIAPRPHNSGHYTIEACLTSQFEQHIRAITGLPLGSSELLIPAV
EEEEEEEECCCCEEEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
MFNLLGEGNGKPKVIGMKEALKFPGVYVHIYGKSVVKPLRKMGHVTVVNRNLEKALEIAH
HHHHHCCCCCCCEEEEHHHHHHCCCEEEEEECHHHHHHHHHCCCEEEECCCHHHHHHHHH
KVKKVIKVVGDA
HHHHHHHHHCCC
>Mature Secondary Structure
MIVGILGGGQLAKMMAQEAKKMGFKVAILDPQEEPPAKGVSDYHILGSFKDEKAIRKLAE
CEEEEECCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHH
ISDVLTYDIEHINVQALKELEKEGVQIYPSPRVLEIIQDKLVQMQVMKEAGIPVPRFIKA
HHHHHHHHHHHCCHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHCC
DKDELVDKALEFGFPLVQKTRREGYDGKGVAVIRDKSELAKLIPADSMLQEFVNIEKEIA
CHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEECHHHHHHHCCHHHHHHHHHCCHHHEE
VMVARDQEGNTVAYPVVEMTFNEANILDMLIAPARIEEEIAEEAKRIAIKAVKALDGVGI
EEEEECCCCCEEEEEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
FGVEMFLTKDGEVYLNEIAPRPHNSGHYTIEACLTSQFEQHIRAITGLPLGSSELLIPAV
EEEEEEEECCCCEEEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
MFNLLGEGNGKPKVIGMKEALKFPGVYVHIYGKSVVKPLRKMGHVTVVNRNLEKALEIAH
HHHHHCCCCCCCEEEEHHHHHHCCCEEEEEECHHHHHHHHHCCCEEEECCCHHHHHHHHH
KVKKVIKVVGDA
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]